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The map label for this gene is sun

Identifier: 27364478

GI number: 27364478

Start: 1048859

End: 1050139

Strand: Reverse

Name: sun

Synonym: VV1_1046

Alternate gene names: 27364478

Gene position: 1050139-1048859 (Counterclockwise)

Preceding gene: 27364479

Following gene: 27364477

Centisome position: 32.0

GC content: 50.43

Gene sequence:

>1281_bases
ATGAATGTTCGCGCTGCGGCTGCCAACGTCTTGTATCAAGTTGTCGACCGAGGCAACTCTCTATCGACCGCCCTTCCTGA
AGCTCAACAATCCATTCGCCCTCGCGACCACGCCTTACTGCAAGAGATCTGCTACGGCGCATTACGCTACCTGCCTCGTT
TAGAGAGCATTGCCGGTAAGTTGATGGATAAACCTCTCAAAGGTAAACAACGTGTTTTTCATCACCTCATTTTGGTTGGC
TTATACCAGTTAGGTCACATGCGTATTCCGGCACACGCGGCGGTTGGTGAAACGGTGGAAGGCGCACAAGCGCTGAAAGG
TCCTCGCTTACGCGGCTTGATCAATGCGGTTTTGCGCAACTATCAGCGTGAACAACAAGCATTGGATGAGTTTTCTGTCA
GCCACAATGCCGGCAAATACGTTCACCCAAGTTGGCTACTGAAAATCCTGCAAGATGCTTACCCTGAGCAATGGCAGACG
ATTGTTGAAGCCAACAATAACAAAGCGCCGATGTGGCTACGCGTGAATCACGCTCATCATGATCGCGACGAATATCTGCA
ACTGCTCAAAAATGCAAACATTGATAGCACTCTGCACAGTGAAGCGATGGACGCCATAAAATTGGCCGCCCCTTGTGATG
TACACAGCTTGCCAGGGTTTGAGCAAGGTTGGGTTTCTGTGCAAGATGCCGCCGCACAGCTGTCGTTTAACTATCTACAA
CCTAAAGATGGCGAACTGATCCTCGATTGCTGCGCGGCACCTGGTGGTAAAACCGCGCACATTTTAGAACGAGTCTCTGC
TCGTGAAGTCGTGGCACTGGACTGCGATGAACAACGCTTGAAAAGAGTGACGGAAAACCTGAAACGCCTCAATCTTCAAG
CAAAAGTGATTTGCGGTGATGCACGCAATCCTGAACAATGGTGGCAAGGCGAGCAATTTGACCGTATTTTGCTCGATGCA
CCTTGCTCTGCAACCGGGGTGATTCGTCGTCACCCAGACATCAAATGGCTGCGCAGAGCAGAAGACATCACTGCGTTAGC
ACAGTTACAAAGCGAAATTTTTGATGCCATGTGGGCACAACTCAAGCCAGGTGGCACCTTGGTTTACGCCACTTGCTCAA
TCACGCCAATGGAAAATGTGGAGCAAGTGAAGGCGTTTCTCGCCAGAACCTCAGCAGCACAACTGGTTGGCTCTGAGCGC
GAGAATCCTGGTCGCCAAATTATTCCCGGAGAGGAAGATATGGATGGCTTCTACTATGCCGTGCTAGTTAAGCAAGCATA
A

Upstream 100 bases:

>100_bases
GCAGTGTACTGAGTTAACCTGCCAAGCAATCGGGCATTTTTGCCCAGTTTAAGAACTTTACGAGGGCAGAGACGCCCTCT
TTAACTTTAAGGTACTCATC

Downstream 100 bases:

>100_bases
GTAAATGGGCGATTGAAGCCTCTTCAGTCGCCTTTTTCGAATCACGAGAACAGAGTATGAAAATCATTATTCTTGGTGCA
GGTCAGGTGGGTGGCACACT

Product: 16S rRNA methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MNVRAAAANVLYQVVDRGNSLSTALPEAQQSIRPRDHALLQEICYGALRYLPRLESIAGKLMDKPLKGKQRVFHHLILVG
LYQLGHMRIPAHAAVGETVEGAQALKGPRLRGLINAVLRNYQREQQALDEFSVSHNAGKYVHPSWLLKILQDAYPEQWQT
IVEANNNKAPMWLRVNHAHHDRDEYLQLLKNANIDSTLHSEAMDAIKLAAPCDVHSLPGFEQGWVSVQDAAAQLSFNYLQ
PKDGELILDCCAAPGGKTAHILERVSAREVVALDCDEQRLKRVTENLKRLNLQAKVICGDARNPEQWWQGEQFDRILLDA
PCSATGVIRRHPDIKWLRRAEDITALAQLQSEIFDAMWAQLKPGGTLVYATCSITPMENVEQVKAFLARTSAAQLVGSER
ENPGRQIIPGEEDMDGFYYAVLVKQA

Sequences:

>Translated_426_residues
MNVRAAAANVLYQVVDRGNSLSTALPEAQQSIRPRDHALLQEICYGALRYLPRLESIAGKLMDKPLKGKQRVFHHLILVG
LYQLGHMRIPAHAAVGETVEGAQALKGPRLRGLINAVLRNYQREQQALDEFSVSHNAGKYVHPSWLLKILQDAYPEQWQT
IVEANNNKAPMWLRVNHAHHDRDEYLQLLKNANIDSTLHSEAMDAIKLAAPCDVHSLPGFEQGWVSVQDAAAQLSFNYLQ
PKDGELILDCCAAPGGKTAHILERVSAREVVALDCDEQRLKRVTENLKRLNLQAKVICGDARNPEQWWQGEQFDRILLDA
PCSATGVIRRHPDIKWLRRAEDITALAQLQSEIFDAMWAQLKPGGTLVYATCSITPMENVEQVKAFLARTSAAQLVGSER
ENPGRQIIPGEEDMDGFYYAVLVKQA
>Mature_426_residues
MNVRAAAANVLYQVVDRGNSLSTALPEAQQSIRPRDHALLQEICYGALRYLPRLESIAGKLMDKPLKGKQRVFHHLILVG
LYQLGHMRIPAHAAVGETVEGAQALKGPRLRGLINAVLRNYQREQQALDEFSVSHNAGKYVHPSWLLKILQDAYPEQWQT
IVEANNNKAPMWLRVNHAHHDRDEYLQLLKNANIDSTLHSEAMDAIKLAAPCDVHSLPGFEQGWVSVQDAAAQLSFNYLQ
PKDGELILDCCAAPGGKTAHILERVSAREVVALDCDEQRLKRVTENLKRLNLQAKVICGDARNPEQWWQGEQFDRILLDA
PCSATGVIRRHPDIKWLRRAEDITALAQLQSEIFDAMWAQLKPGGTLVYATCSITPMENVEQVKAFLARTSAAQLVGSER
ENPGRQIIPGEEDMDGFYYAVLVKQA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family

Homologues:

Organism=Homo sapiens, GI76150625, Length=312, Percent_Identity=33.6538461538462, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI76150623, Length=312, Percent_Identity=33.6538461538462, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI32698918, Length=207, Percent_Identity=34.7826086956522, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI39995082, Length=209, Percent_Identity=31.5789473684211, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI301336155, Length=209, Percent_Identity=31.5789473684211, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI40316918, Length=184, Percent_Identity=30.9782608695652, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI11545785, Length=173, Percent_Identity=32.3699421965318, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI2367212, Length=426, Percent_Identity=59.6244131455399, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87081985, Length=226, Percent_Identity=31.4159292035398, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=313, Percent_Identity=28.7539936102236, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25143471, Length=296, Percent_Identity=29.3918918918919, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324268, Length=310, Percent_Identity=28.0645161290323, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319447, Length=300, Percent_Identity=28, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI22024126, Length=312, Percent_Identity=29.1666666666667, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19920494, Length=164, Percent_Identity=32.9268292682927, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355201, Length=211, Percent_Identity=30.3317535545024, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21356513, Length=164, Percent_Identity=33.5365853658537, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24668781, Length=164, Percent_Identity=34.7560975609756, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMB_VIBVU (Q8DDE5)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760006.1
- ProteinModelPortal:   Q8DDE5
- SMR:   Q8DDE5
- GeneID:   1177979
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1046
- HOGENOM:   HBG726909
- OMA:   ANNAHPP
- ProtClustDB:   PRK10901
- BioCyc:   VVUL216895:VV1_1046-MONOMER
- GO:   GO:0005737
- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR00563

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: =2.1.1.176

Molecular weight: Translated: 47678; Mature: 47678

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: ACT_SITE 372-372 BINDING 274-274 BINDING 300-300 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRAAAANVLYQVVDRGNSLSTALPEAQQSIRPRDHALLQEICYGALRYLPRLESIAGK
CCCHHHHHHHHHHHHHCCCCCHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMDKPLKGKQRVFHHLILVGLYQLGHMRIPAHAAVGETVEGAQALKGPRLRGLINAVLRN
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHCCCHHHHHHHHHHHH
YQREQQALDEFSVSHNAGKYVHPSWLLKILQDAYPEQWQTIVEANNNKAPMWLRVNHAHH
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEECCCCC
DRDEYLQLLKNANIDSTLHSEAMDAIKLAAPCDVHSLPGFEQGWVSVQDAAAQLSFNYLQ
CHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
PKDGELILDCCAAPGGKTAHILERVSAREVVALDCDEQRLKRVTENLKRLNLQAKVICGD
CCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHCCEEEEEECC
ARNPEQWWQGEQFDRILLDAPCSATGVIRRHPDIKWLRRAEDITALAQLQSEIFDAMWAQ
CCCHHHHCCCCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKPGGTLVYATCSITPMENVEQVKAFLARTSAAQLVGSERENPGRQIIPGEEDMDGFYYA
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
VLVKQA
EEEECC
>Mature Secondary Structure
MNVRAAAANVLYQVVDRGNSLSTALPEAQQSIRPRDHALLQEICYGALRYLPRLESIAGK
CCCHHHHHHHHHHHHHCCCCCHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMDKPLKGKQRVFHHLILVGLYQLGHMRIPAHAAVGETVEGAQALKGPRLRGLINAVLRN
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHCCCHHHHHHHHHHHH
YQREQQALDEFSVSHNAGKYVHPSWLLKILQDAYPEQWQTIVEANNNKAPMWLRVNHAHH
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCEEEEECCCCC
DRDEYLQLLKNANIDSTLHSEAMDAIKLAAPCDVHSLPGFEQGWVSVQDAAAQLSFNYLQ
CHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
PKDGELILDCCAAPGGKTAHILERVSAREVVALDCDEQRLKRVTENLKRLNLQAKVICGD
CCCCCEEEEECCCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHCCEEEEEECC
ARNPEQWWQGEQFDRILLDAPCSATGVIRRHPDIKWLRRAEDITALAQLQSEIFDAMWAQ
CCCHHHHCCCCCCCEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKPGGTLVYATCSITPMENVEQVKAFLARTSAAQLVGSERENPGRQIIPGEEDMDGFYYA
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
VLVKQA
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA