Click here to switch to the map view.

The map label for this gene is ilvD [H]

Identifier: 27364462

GI number: 27364462

Start: 1031260

End: 1033101

Strand: Reverse

Name: ilvD [H]

Synonym: VV1_1029

Alternate gene names: 27364462

Gene position: 1033101-1031260 (Counterclockwise)

Preceding gene: 27364463

Following gene: 27364461

Centisome position: 31.48

GC content: 54.02

Gene sequence:

>1842_bases
ATGCCTAAATACAGATCAGCCACCACCACTCATGGTCGCAACATGGCCGGTGCACGTGCACTATGGCGTGCTACGGGCGT
AAAAGATGAAGACTTCGGCAAACCGATTATTGCCGTGGTCAACTCCTTTACCCAGTTCGTACCGGGCCACGTGCACCTGA
AAGACCTTGGTCAATTGGTGGCAAGAGAAATTGAAGCAGCAGGCGGCATTGCCAAAGAATTCAACACCATCGCGGTGGAT
GATGGTATCGCCATGGGCCACGGAGGCATGCTTTATTCACTGCCTTCTCGTGAATTGATTGCCGACTCGGTGGAGTACAT
GGTCAACGCGCACTGTGCCGATGCCATGGTGTGTATCTCCAACTGTGACAAAATCACCCCGGGAATGCTGATGGCGTCAA
TGCGCCTTAATATCCCGGTGATCTTTGTCTCGGGTGGACCAATGGAAGCGGGCAAAACTAAGCTTTCGGATCAGATCATC
AAGCTGGATCTGGTCGATGCCATGATCCAAGGCGCGGATCCTAAAGTCTCGGATGAGCAGAGCGAACAGATCGAGCGCAG
TGCTTGCCCTACCTGTGGATCCTGCTCAGGTATGTTCACCGCCAACTCGATGAACTGCCTCACCGAAGCGCTTGGCCTTT
CTCAGCCAGGTAACGGCTCTTTGCTCGCAACGCATGCCGATCGTAAACAACTGTTTATCAGTGCAGGCCAACGCATTGTC
GAACTGACCAAGCGTTACTACGAGCAAGATGATGAAAGCGCACTGCCGCGCAACATTGCGACCAAAGCCGCGTTTGAAAA
CGCCATGGCGCTAGACATCGCCATGGGCGGCTCAACCAACACCGTTTTACACCTCTTAGCCGCAGCGCAAGAAGGTGAAG
TGGATTTTGACATGACAGACATCGATCGCATGTCACGCATGGTGCCTCACCTATGTAAAGTGGCGCCATCAACCCAGAAA
TACCACATGGAAGATGTGCACCGCGCAGGTGGTGTGGTCGGTATTTTGGGCGAACTTAACCGAGCGGGCCTGCTGCACAA
TCAATCGAAAACGGTGCTTGGCCTCACTTGGGAAGAACAGCTTGCCCAATACGACATCATGCTGACCGATTCGGAAGAGG
TGAAACGCTTCTACCGTGCAGGCCCTGCGGGTATTCGTACCACCCAAGCATTCTCGCAAGATTGCCGCTGGGATACCTTG
GATGACGATCGCGCCCAAGGCTGTATTCGCACCAAAGAAAACGCCTTTAGCCAAGATGGTGGCCTTGCTGTCCTCAAAGG
CAATATTGCGCTGGATGGCTGTATCGTTAAGACGGCGGGTGTGGATGAAAGCATTCTAAAATTCACTGGCCCTGCTGTGG
TTTTTGAAAGCCAAGAAGACGCGGTGGAAGGTATCTTGGGTGGCAAAGTCAAAGCGGGCGATGTGGTGGTGATTCGCTAC
GAAGGCCCGAAAGGCGGCCCAGGCATGCAAGAGATGCTCTACCCTACCACGTATTTGAAATCGATGGGTCTAGGCAAAGC
GTGCGCGCTACTGACCGACGGACGTTTCTCTGGCGGCACATCGGGTCTTTCCATCGGCCACGCCTCACCAGAAGCGGCCA
ACGGCGGCGCGATCGGTTTGGTCAAACAGGGCGACTTGATTGCCATCGACATTCCAAATCGCACGATTTCTCTGCAAGTT
TCCGATCAAGAAATGGCAGAGCGCCGAGCAGAACAAGACGCGCTTGGCTGGAAACCCGTCTCTCGCCAACGCGAAGTGTC
GTTTGCACTGAAAGCCTACGCCAGCATGGCAACCAGTGCCGACAAAGGTGCGGTAAGAGACAAGTCAAAGCTTGAGGGCT
AA

Upstream 100 bases:

>100_bases
GGTTCAAGAAGCCTACTTTGGCTTATTCAATGGCACCACCGAAGATAAGTGGGGCTGGTTAGATTACGTGTACCCAGAGA
AGAAGTAGGGAGACAACAGA

Downstream 100 bases:

>100_bases
GCAATGTCATTAACCCAACAAACTGGCGCAGACTACCTGCGCCAGATACTACGCGCTCCTGTGTATGAAGTGGCGACCGT
CACCCCCTTGCAAGAGATGC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MPKYRSATTTHGRNMAGARALWRATGVKDEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGIAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASMRLNIPVIFVSGGPMEAGKTKLSDQII
KLDLVDAMIQGADPKVSDEQSEQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFISAGQRIV
ELTKRYYEQDDESALPRNIATKAAFENAMALDIAMGGSTNTVLHLLAAAQEGEVDFDMTDIDRMSRMVPHLCKVAPSTQK
YHMEDVHRAGGVVGILGELNRAGLLHNQSKTVLGLTWEEQLAQYDIMLTDSEEVKRFYRAGPAGIRTTQAFSQDCRWDTL
DDDRAQGCIRTKENAFSQDGGLAVLKGNIALDGCIVKTAGVDESILKFTGPAVVFESQEDAVEGILGGKVKAGDVVVIRY
EGPKGGPGMQEMLYPTTYLKSMGLGKACALLTDGRFSGGTSGLSIGHASPEAANGGAIGLVKQGDLIAIDIPNRTISLQV
SDQEMAERRAEQDALGWKPVSRQREVSFALKAYASMATSADKGAVRDKSKLEG

Sequences:

>Translated_613_residues
MPKYRSATTTHGRNMAGARALWRATGVKDEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGIAKEFNTIAVD
DGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASMRLNIPVIFVSGGPMEAGKTKLSDQII
KLDLVDAMIQGADPKVSDEQSEQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFISAGQRIV
ELTKRYYEQDDESALPRNIATKAAFENAMALDIAMGGSTNTVLHLLAAAQEGEVDFDMTDIDRMSRMVPHLCKVAPSTQK
YHMEDVHRAGGVVGILGELNRAGLLHNQSKTVLGLTWEEQLAQYDIMLTDSEEVKRFYRAGPAGIRTTQAFSQDCRWDTL
DDDRAQGCIRTKENAFSQDGGLAVLKGNIALDGCIVKTAGVDESILKFTGPAVVFESQEDAVEGILGGKVKAGDVVVIRY
EGPKGGPGMQEMLYPTTYLKSMGLGKACALLTDGRFSGGTSGLSIGHASPEAANGGAIGLVKQGDLIAIDIPNRTISLQV
SDQEMAERRAEQDALGWKPVSRQREVSFALKAYASMATSADKGAVRDKSKLEG
>Mature_612_residues
PKYRSATTTHGRNMAGARALWRATGVKDEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGIAKEFNTIAVDD
GIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCISNCDKITPGMLMASMRLNIPVIFVSGGPMEAGKTKLSDQIIK
LDLVDAMIQGADPKVSDEQSEQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFISAGQRIVE
LTKRYYEQDDESALPRNIATKAAFENAMALDIAMGGSTNTVLHLLAAAQEGEVDFDMTDIDRMSRMVPHLCKVAPSTQKY
HMEDVHRAGGVVGILGELNRAGLLHNQSKTVLGLTWEEQLAQYDIMLTDSEEVKRFYRAGPAGIRTTQAFSQDCRWDTLD
DDRAQGCIRTKENAFSQDGGLAVLKGNIALDGCIVKTAGVDESILKFTGPAVVFESQEDAVEGILGGKVKAGDVVVIRYE
GPKGGPGMQEMLYPTTYLKSMGLGKACALLTDGRFSGGTSGLSIGHASPEAANGGAIGLVKQGDLIAIDIPNRTISLQVS
DQEMAERRAEQDALGWKPVSRQREVSFALKAYASMATSADKGAVRDKSKLEG

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=616, Percent_Identity=85.3896103896104, Blast_Score=1100, Evalue=0.0,
Organism=Escherichia coli, GI1788157, Length=574, Percent_Identity=28.397212543554, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI2367371, Length=570, Percent_Identity=28.4210526315789, Blast_Score=175, Evalue=7e-45,
Organism=Escherichia coli, GI1786464, Length=529, Percent_Identity=30.2457466918715, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6322476, Length=612, Percent_Identity=38.0718954248366, Blast_Score=348, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 65593; Mature: 65461

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYRSATTTHGRNMAGARALWRATGVKDEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEAAGGIAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASMRLNIPVIFVSGGPMEAGKTKLSDQIIKLDLVDAMIQGADPKVSDEQ
CCCCCCHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
SEQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFISAGQRIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHH
ELTKRYYEQDDESALPRNIATKAAFENAMALDIAMGGSTNTVLHLLAAAQEGEVDFDMTD
HHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCHHH
IDRMSRMVPHLCKVAPSTQKYHMEDVHRAGGVVGILGELNRAGLLHNQSKTVLGLTWEEQ
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEECCCCEEEEEEHHHH
LAQYDIMLTDSEEVKRFYRAGPAGIRTTQAFSQDCRWDTLDDDRAQGCIRTKENAFSQDG
HHHEEEEEECHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHCCCHHCHHHCCCCCC
GLAVLKGNIALDGCIVKTAGVDESILKFTGPAVVFESQEDAVEGILGGKVKAGDVVVIRY
CEEEEECCEEEEEEEEEECCCCHHHHHHCCCEEEEECCHHHHHHHHCCCEECCCEEEEEE
EGPKGGPGMQEMLYPTTYLKSMGLGKACALLTDGRFSGGTSGLSIGHASPEAANGGAIGL
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
VKQGDLIAIDIPNRTISLQVSDQEMAERRAEQDALGWKPVSRQREVSFALKAYASMATSA
EECCCEEEEECCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
DKGAVRDKSKLEG
CCCCCCCHHHCCC
>Mature Secondary Structure 
PKYRSATTTHGRNMAGARALWRATGVKDEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEAAGGIAKEFNTIAVDDGIAMGHGGMLYSLPSRELIADSVEYMVNAHCADAMVCIS
HHHHHHHCCHHHHCCEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASMRLNIPVIFVSGGPMEAGKTKLSDQIIKLDLVDAMIQGADPKVSDEQ
CCCCCCHHHHHHHEECCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
SEQIERSACPTCGSCSGMFTANSMNCLTEALGLSQPGNGSLLATHADRKQLFISAGQRIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHH
ELTKRYYEQDDESALPRNIATKAAFENAMALDIAMGGSTNTVLHLLAAAQEGEVDFDMTD
HHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCHHH
IDRMSRMVPHLCKVAPSTQKYHMEDVHRAGGVVGILGELNRAGLLHNQSKTVLGLTWEEQ
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCEECCCCEEEEEEHHHH
LAQYDIMLTDSEEVKRFYRAGPAGIRTTQAFSQDCRWDTLDDDRAQGCIRTKENAFSQDG
HHHEEEEEECHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHCCCHHCHHHCCCCCC
GLAVLKGNIALDGCIVKTAGVDESILKFTGPAVVFESQEDAVEGILGGKVKAGDVVVIRY
CEEEEECCEEEEEEEEEECCCCHHHHHHCCCEEEEECCHHHHHHHHCCCEECCCEEEEEE
EGPKGGPGMQEMLYPTTYLKSMGLGKACALLTDGRFSGGTSGLSIGHASPEAANGGAIGL
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
VKQGDLIAIDIPNRTISLQVSDQEMAERRAEQDALGWKPVSRQREVSFALKAYASMATSA
EECCCEEEEECCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
DKGAVRDKSKLEG
CCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]