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The map label for this gene is ilvE [H]

Identifier: 27364463

GI number: 27364463

Start: 1033114

End: 1034052

Strand: Reverse

Name: ilvE [H]

Synonym: VV1_1030

Alternate gene names: 27364463

Gene position: 1034052-1033114 (Counterclockwise)

Preceding gene: 27364464

Following gene: 27364462

Centisome position: 31.51

GC content: 51.76

Gene sequence:

>939_bases
ATGGCAACGAAAACAGCAGATTATATCTGGTTTAATGGCAAGATGGTTCCCTGGGCAGAAGCGAATGTACACGTTCTGAC
TCACGCCATGCACTACGGCACCTCGGTATTTGAAGGGGTTCGCTGTTACAACACGCCAAAAGGGCCGATTGTCTTCCGTC
ACCAAGAACACGCGCAGCGTCTGAAAGATTCTGCCAAAATTTACCGCTTCCCAATCCCCTTTTCGGTGGAAGAGATCATG
GAAGCGACTCGCGAAACTCTGCGTCAAAACAAGCTGGATAACGCCTACATTCGCCCACTGGCGTTTGTTGGCAACGTAGG
CTTGGGCGTTTGTCCTCCCACAGATACCGAAATGGATCTGATCATTGCCGCCTTCCCTTGGGGCTCTTACTTAGGGGAAG
AAGCGTTGGAAAATGGCGTTGATGCCATGGTATCCAGCTGGAACCGCGCTGCGCCAAACACGATTCCAACCGCAGCGAAA
GCAGGCGGTAACTACCTCTCTTCTCTACTGGTTGGTGGTGAAGCACGTCGACATGGCTATGCCGAAGGGATTGCATTAAG
TGTTGATGGTTACCTTTCAGAAGGTGCCGGTGAAAACATTTTCGTGATCAAAAATGGCGTCATCACCACACCACCAGCGA
CGGCTGCGATTCTCCCAGGCATCACTCGTGACTCAATCATGACACTCGCGCGTGAGATGGGTTATGAAGTCCGTGAAGCC
AACATCGCACGTGAAGCTCTCTACTTGGCAGACGAAGTGTTTATGACGGGCACTGCCGCAGAAGTGGTTCCGGTGCGTAG
TGTCGATCAAATTCAAGTGGGCGCAGGCAAACGCGGCCCAATCACCAAAGCGGTTCAAGAAGCCTACTTTGGCTTATTCA
ATGGCACCACCGAAGATAAGTGGGGCTGGTTAGATTACGTGTACCCAGAGAAGAAGTAG

Upstream 100 bases:

>100_bases
AATTGTGGGATATTCGTAGTGTTGAGGTGATGAAGATTCGTAACGACGAACTCCCTAACAACAATCTACAACAACACGTA
AGTGCATAAGGAAGGCAATA

Downstream 100 bases:

>100_bases
GGAGACAACAGAATGCCTAAATACAGATCAGCCACCACCACTCATGGTCGCAACATGGCCGGTGCACGTGCACTATGGCG
TGCTACGGGCGTAAAAGATG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MATKTADYIWFNGKMVPWAEANVHVLTHAMHYGTSVFEGVRCYNTPKGPIVFRHQEHAQRLKDSAKIYRFPIPFSVEEIM
EATRETLRQNKLDNAYIRPLAFVGNVGLGVCPPTDTEMDLIIAAFPWGSYLGEEALENGVDAMVSSWNRAAPNTIPTAAK
AGGNYLSSLLVGGEARRHGYAEGIALSVDGYLSEGAGENIFVIKNGVITTPPATAAILPGITRDSIMTLAREMGYEVREA
NIAREALYLADEVFMTGTAAEVVPVRSVDQIQVGAGKRGPITKAVQEAYFGLFNGTTEDKWGWLDYVYPEKK

Sequences:

>Translated_312_residues
MATKTADYIWFNGKMVPWAEANVHVLTHAMHYGTSVFEGVRCYNTPKGPIVFRHQEHAQRLKDSAKIYRFPIPFSVEEIM
EATRETLRQNKLDNAYIRPLAFVGNVGLGVCPPTDTEMDLIIAAFPWGSYLGEEALENGVDAMVSSWNRAAPNTIPTAAK
AGGNYLSSLLVGGEARRHGYAEGIALSVDGYLSEGAGENIFVIKNGVITTPPATAAILPGITRDSIMTLAREMGYEVREA
NIAREALYLADEVFMTGTAAEVVPVRSVDQIQVGAGKRGPITKAVQEAYFGLFNGTTEDKWGWLDYVYPEKK
>Mature_311_residues
ATKTADYIWFNGKMVPWAEANVHVLTHAMHYGTSVFEGVRCYNTPKGPIVFRHQEHAQRLKDSAKIYRFPIPFSVEEIME
ATRETLRQNKLDNAYIRPLAFVGNVGLGVCPPTDTEMDLIIAAFPWGSYLGEEALENGVDAMVSSWNRAAPNTIPTAAKA
GGNYLSSLLVGGEARRHGYAEGIALSVDGYLSEGAGENIFVIKNGVITTPPATAAILPGITRDSIMTLAREMGYEVREAN
IAREALYLADEVFMTGTAAEVVPVRSVDQIQVGAGKRGPITKAVQEAYFGLFNGTTEDKWGWLDYVYPEKK

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI50658084, Length=288, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI296010904, Length=308, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI38176287, Length=308, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI296010906, Length=308, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI258614015, Length=260, Percent_Identity=30, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI48994963, Length=307, Percent_Identity=71.6612377850163, Blast_Score=483, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17565728, Length=283, Percent_Identity=30.3886925795053, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17568601, Length=284, Percent_Identity=30.9859154929577, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322002, Length=289, Percent_Identity=28.3737024221453, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6322608, Length=330, Percent_Identity=26.969696969697, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24641779, Length=324, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34184; Mature: 34053

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKTADYIWFNGKMVPWAEANVHVLTHAMHYGTSVFEGVRCYNTPKGPIVFRHQEHAQR
CCCCCCCEEEECCEECCCCCCCHHHHHHHHHHCHHHHHCHHCCCCCCCCEEEECHHHHHH
LKDSAKIYRFPIPFSVEEIMEATRETLRQNKLDNAYIRPLAFVGNVGLGVCPPTDTEMDL
HHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEE
IIAAFPWGSYLGEEALENGVDAMVSSWNRAAPNTIPTAAKAGGNYLSSLLVGGEARRHGY
EEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHCCHHHHCCC
AEGIALSVDGYLSEGAGENIFVIKNGVITTPPATAAILPGITRDSIMTLAREMGYEVREA
CCCEEEEECCHHCCCCCCEEEEEECCEEECCCCHHHHCCCCCHHHHHHHHHHHCCHHHHH
NIAREALYLADEVFMTGTAAEVVPVRSVDQIQVGAGKRGPITKAVQEAYFGLFNGTTEDK
HHHHHHHHHHHHHHCCCCCHHEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCC
WGWLDYVYPEKK
CCCEEECCCCCC
>Mature Secondary Structure 
ATKTADYIWFNGKMVPWAEANVHVLTHAMHYGTSVFEGVRCYNTPKGPIVFRHQEHAQR
CCCCCCEEEECCEECCCCCCCHHHHHHHHHHCHHHHHCHHCCCCCCCCEEEECHHHHHH
LKDSAKIYRFPIPFSVEEIMEATRETLRQNKLDNAYIRPLAFVGNVGLGVCPPTDTEMDL
HHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEE
IIAAFPWGSYLGEEALENGVDAMVSSWNRAAPNTIPTAAKAGGNYLSSLLVGGEARRHGY
EEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHCCHHHHCCC
AEGIALSVDGYLSEGAGENIFVIKNGVITTPPATAAILPGITRDSIMTLAREMGYEVREA
CCCEEEEECCHHCCCCCCEEEEEECCEEECCCCHHHHCCCCCHHHHHHHHHHHCCHHHHH
NIAREALYLADEVFMTGTAAEVVPVRSVDQIQVGAGKRGPITKAVQEAYFGLFNGTTEDK
HHHHHHHHHHHHHHCCCCCHHEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCC
WGWLDYVYPEKK
CCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]