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The map label for this gene is tgt [H]

Identifier: 27363913

GI number: 27363913

Start: 440695

End: 441933

Strand: Reverse

Name: tgt [H]

Synonym: VV1_0445

Alternate gene names: 27363913

Gene position: 441933-440695 (Counterclockwise)

Preceding gene: 27363914

Following gene: 27363912

Centisome position: 13.47

GC content: 46.65

Gene sequence:

>1239_bases
ATGGATTACTCCATTTTTTCGCAAGGAAACCGCGACCGCTCTGGTGGGACGGAAACGTCCGTGACAATACCGTCAGACTG
TTTCTCTGACGTTGGAGGCTTCGTGAAATTAAAATTTGATCTTAAAAAGAAAAATGGCAATGCCCGTCGTGGTCAATTGA
CATTTGAACGTGGAACTGTGCAAACCCCGGCGTTTATGCCTGTAGGTACTTACGGTACCGTCAAAGGTATGACACCAGAA
GAAGTAAAAGAGACGGGTGCAGAAATTCTACTAGGCAACACATTCCACTTATGGCTACGTCCTGGCCAAGAAGTGATGAA
AATGCACGGCGATCTGCACGATTTCATGAACTGGCAAGGTCCTATCTTGACTGACTCAGGCGGTTTCCAAGTATTCAGCC
TTGGTGATATTCGTAAAATTACCGAAGAAGGTGTGCATTTCCGCAACCCAGTAAACGGTGACAAGATTTTCATGGATGCT
GAGAAATCGATGGAAATTCAAAAAGACCTAGGTTCTGACATCGTAATGATTTTCGATGAGTGTACGCCTTACCCTGCGAC
TCACGCTGAAGCGAAAAAATCGATGGAAATGTCTCTGCGTTGGGCACAACGCTCGCGTGACCATTTCGATAAGCTCGAAA
ACCCAAACAACCTGTTTGGCATTGTTCAAGGCGGTGTCTACGAAGATCTTCGTGATGTGTCAGTTAAAGGCCTAACAGAG
ATTGGTTTTGATGGCTATGCGGTCGGTGGTCTTGCGGTTGGCGAACCTAAAGAAGACATGCATCGCGTACTTGAGCACAC
CTGTCCTCAGTTACCAGAAGATAAACCGCGTTACCTGATGGGCGTGGGCAAACCAGAAGACTTGGTTGAAGGGGTTCGTC
GCGGTATCGACATGTTTGACTGTGTAATGCCAACGCGAAATGCGCGTAATGGACACCTATTTGTGACTGGTGGTGTGATC
AAGATCCGTAATGCGACACATAAAACGGATACAACCCCTCTCGATCCGCACTGTGACTGTTACACTTGCAAAAATTACTC
TAAGTCGTATCTGCACCACTTGGACCGTTGTAATGAAATCCTTGGTGCTCGCTTAAACACTATCCATAACCTGCGTTATT
ACCAACGTCTCATGGAAAGCATTCGTAAAGCGATTGATGAAGATCGCTTCGAGCAGTTTGTGGAAGAGTTCTACGCTCGA
CGCAATCGCGAAGTGCCACCACTTGGTAAACAGGCATAA

Upstream 100 bases:

>100_bases
CAACAAATGTTATCTAAAGTGAAGTAAGTTTCATTTTAGTGGTGCAGATCGGGATAATCTTGGCTGCATGCAAAACGAGT
GCTAGCAGCAGGCTAGAGGG

Downstream 100 bases:

>100_bases
GTTCGCAATAGTAAAAAAGTGTTACGCCATCTTGTTTTTGTGAGTGAACTCGCTTGAAAATGAGGTGGCGTTACCCAATT
TATTGGATTATTAATAAAAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MDYSIFSQGNRDRSGGTETSVTIPSDCFSDVGGFVKLKFDLKKKNGNARRGQLTFERGTVQTPAFMPVGTYGTVKGMTPE
EVKETGAEILLGNTFHLWLRPGQEVMKMHGDLHDFMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDA
EKSMEIQKDLGSDIVMIFDECTPYPATHAEAKKSMEMSLRWAQRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTE
IGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPEDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVI
KIRNATHKTDTTPLDPHCDCYTCKNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFEQFVEEFYAR
RNREVPPLGKQA

Sequences:

>Translated_412_residues
MDYSIFSQGNRDRSGGTETSVTIPSDCFSDVGGFVKLKFDLKKKNGNARRGQLTFERGTVQTPAFMPVGTYGTVKGMTPE
EVKETGAEILLGNTFHLWLRPGQEVMKMHGDLHDFMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDA
EKSMEIQKDLGSDIVMIFDECTPYPATHAEAKKSMEMSLRWAQRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTE
IGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPEDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVI
KIRNATHKTDTTPLDPHCDCYTCKNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFEQFVEEFYAR
RNREVPPLGKQA
>Mature_412_residues
MDYSIFSQGNRDRSGGTETSVTIPSDCFSDVGGFVKLKFDLKKKNGNARRGQLTFERGTVQTPAFMPVGTYGTVKGMTPE
EVKETGAEILLGNTFHLWLRPGQEVMKMHGDLHDFMNWQGPILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDA
EKSMEIQKDLGSDIVMIFDECTPYPATHAEAKKSMEMSLRWAQRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTE
IGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPEDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTGGVI
KIRNATHKTDTTPLDPHCDCYTCKNYSKSYLHHLDRCNEILGARLNTIHNLRYYQRLMESIRKAIDEDRFEQFVEEFYAR
RNREVPPLGKQA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=350, Percent_Identity=44, Blast_Score=311, Evalue=7e-85,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=80.6451612903226, Blast_Score=651, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=41.7127071823204, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI25146502, Length=163, Percent_Identity=33.7423312883436, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19920510, Length=361, Percent_Identity=45.983379501385, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI21355311, Length=257, Percent_Identity=28.7937743190661, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 46685; Mature: 46685

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYSIFSQGNRDRSGGTETSVTIPSDCFSDVGGFVKLKFDLKKKNGNARRGQLTFERGTV
CCCCCCCCCCCCCCCCCCCEEECCHHHHHCCCCEEEEEEEEEECCCCCCCCEEEEECCCC
QTPAFMPVGTYGTVKGMTPEEVKETGAEILLGNTFHLWLRPGQEVMKMHGDLHDFMNWQG
CCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCEEEEEECCCHHHHHHHCCHHHHHCCCC
PILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDE
CEEECCCCEEEEECCHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEC
CTPYPATHAEAKKSMEMSLRWAQRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCHHH
IGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPEDKPRYLMGVGKPEDLVEGVRRGIDMFD
CCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHH
CVMPTRNARNGHLFVTGGVIKIRNATHKTDTTPLDPHCDCYTCKNYSKSYLHHLDRCNEI
HHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHH
LGARLNTIHNLRYYQRLMESIRKAIDEDRFEQFVEEFYARRNREVPPLGKQA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDYSIFSQGNRDRSGGTETSVTIPSDCFSDVGGFVKLKFDLKKKNGNARRGQLTFERGTV
CCCCCCCCCCCCCCCCCCCEEECCHHHHHCCCCEEEEEEEEEECCCCCCCCEEEEECCCC
QTPAFMPVGTYGTVKGMTPEEVKETGAEILLGNTFHLWLRPGQEVMKMHGDLHDFMNWQG
CCCCCEECCCCCCCCCCCHHHHHHCCCEEEECCEEEEEECCCHHHHHHHCCHHHHHCCCC
PILTDSGGFQVFSLGDIRKITEEGVHFRNPVNGDKIFMDAEKSMEIQKDLGSDIVMIFDE
CEEECCCCEEEEECCHHHHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCEEEEEEC
CTPYPATHAEAKKSMEMSLRWAQRSRDHFDKLENPNNLFGIVQGGVYEDLRDVSVKGLTE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCCCCCHHH
IGFDGYAVGGLAVGEPKEDMHRVLEHTCPQLPEDKPRYLMGVGKPEDLVEGVRRGIDMFD
CCCCCEEECCEECCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHH
CVMPTRNARNGHLFVTGGVIKIRNATHKTDTTPLDPHCDCYTCKNYSKSYLHHLDRCNEI
HHCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHH
LGARLNTIHNLRYYQRLMESIRKAIDEDRFEQFVEEFYARRNREVPPLGKQA
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA