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The map label for this gene is queA

Identifier: 27363914

GI number: 27363914

Start: 442047

End: 443099

Strand: Reverse

Name: queA

Synonym: VV1_0446

Alternate gene names: 27363914

Gene position: 443099-442047 (Counterclockwise)

Preceding gene: 326423713

Following gene: 27363913

Centisome position: 13.5

GC content: 48.62

Gene sequence:

>1053_bases
ATGCAAGTTTCAGATTTTCACTTTGACTTACCTGACGAACTGATCGCTCGTTATCCTCAATCGGAGCGTACCGCCAGTCG
TCTACTTCAGCTAAATGGCAACACCGGCGCTGTAAAAGATGGCTCGTTCAAAGATGTGTTGGAGCTGGTTCAAGCAGGGG
ATCTTTTGGTGTTTAACAACACGCGAGTGATTCCTGCACGCATGTTTGGTCGTAAAGAATCTGGCGGCAAGTTGGAAGTC
TTGGTCGAGCGCATGCTTGATGAGAAACGCTTCCTCGCGCATGTACGCAGTTCTAAGTCTCCGAAACCGGGTACGTTAGT
CTTTCTTGGGGAAGAAGACCAATACTCAGCAGAGATGGTGGCGCGCCAAGATGCGTTGTTTGAGCTCCATTTAAAGGCAG
ACAAAACCATTCTCGAAGTGTTGGAAGAAATTGGCCATATGCCTCTTCCACCTTACATTGACCGCCCAGATGAGGATGCG
GATAAAGAGCGTTACCAAACGGTTTATAACCAAAAACCGGGTGCGGTTGCTGCACCGACGGCGGGATTGCATTTTGACAA
TCAGCTGCTTGAGCAGATCAAAGCCAAAGGCGCTGAGTTTGCGTACGTGACGTTGCATGTCGGTGCTGGCACATTTCAGC
CAGTGAAAGTGGACAATATTCTTGAGCATCACATGCACTCTGAGTACGCAGAAGTACCACAAGAGGTGGTGGATGCGATT
AACGCCACGAAAGCACGTGGTGGCCGCGTCATTGCGGTTGGTACCACGTCAGTACGCTCTCTAGAGAGCGCGGCGCAAGA
GTCGTTGAAAAACGGCACAGAGTTGATGCCTTTCTTTGGCGATACGGAAATCTTCATTTTCCCTGGCTATCAATATCAGT
TGGTGGACTGTTTGATCACCAATTTCCATTTGCCAGAATCGACGCTGATTATGCTGGTGAGTGCATTTGCCGGTTATGAC
CATACCATGAATGCGTATCAGCATGCCGTGTCCAATCAATATCGTTTTTTCTCTTACGGGGATGCGATGTTCATTGAGAA
GAAAACGCAATAA

Upstream 100 bases:

>100_bases
TTTCTTGCTATTGACTGGTCTCGCTCTATACTAGCCCACTGCGAAAATTTTACTCGCAACAGAATTCCTTTATTTCGACA
AATTTACCAAGAGCAGTAGC

Downstream 100 bases:

>100_bases
GCCATAAAAAGCGCAACAAATGTTATCTAAAGTGAAGTAAGTTTCATTTTAGTGGTGCAGATCGGGATAATCTTGGCTGC
ATGCAAAACGAGTGCTAGCA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MQVSDFHFDLPDELIARYPQSERTASRLLQLNGNTGAVKDGSFKDVLELVQAGDLLVFNNTRVIPARMFGRKESGGKLEV
LVERMLDEKRFLAHVRSSKSPKPGTLVFLGEEDQYSAEMVARQDALFELHLKADKTILEVLEEIGHMPLPPYIDRPDEDA
DKERYQTVYNQKPGAVAAPTAGLHFDNQLLEQIKAKGAEFAYVTLHVGAGTFQPVKVDNILEHHMHSEYAEVPQEVVDAI
NATKARGGRVIAVGTTSVRSLESAAQESLKNGTELMPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYD
HTMNAYQHAVSNQYRFFSYGDAMFIEKKTQ

Sequences:

>Translated_350_residues
MQVSDFHFDLPDELIARYPQSERTASRLLQLNGNTGAVKDGSFKDVLELVQAGDLLVFNNTRVIPARMFGRKESGGKLEV
LVERMLDEKRFLAHVRSSKSPKPGTLVFLGEEDQYSAEMVARQDALFELHLKADKTILEVLEEIGHMPLPPYIDRPDEDA
DKERYQTVYNQKPGAVAAPTAGLHFDNQLLEQIKAKGAEFAYVTLHVGAGTFQPVKVDNILEHHMHSEYAEVPQEVVDAI
NATKARGGRVIAVGTTSVRSLESAAQESLKNGTELMPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYD
HTMNAYQHAVSNQYRFFSYGDAMFIEKKTQ
>Mature_350_residues
MQVSDFHFDLPDELIARYPQSERTASRLLQLNGNTGAVKDGSFKDVLELVQAGDLLVFNNTRVIPARMFGRKESGGKLEV
LVERMLDEKRFLAHVRSSKSPKPGTLVFLGEEDQYSAEMVARQDALFELHLKADKTILEVLEEIGHMPLPPYIDRPDEDA
DKERYQTVYNQKPGAVAAPTAGLHFDNQLLEQIKAKGAEFAYVTLHVGAGTFQPVKVDNILEHHMHSEYAEVPQEVVDAI
NATKARGGRVIAVGTTSVRSLESAAQESLKNGTELMPFFGDTEIFIFPGYQYQLVDCLITNFHLPESTLIMLVSAFAGYD
HTMNAYQHAVSNQYRFFSYGDAMFIEKKTQ

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=350, Percent_Identity=68.5714285714286, Blast_Score=509, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_VIBVU (Q8DEX9)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759442.1
- ProteinModelPortal:   Q8DEX9
- SMR:   Q8DEX9
- GeneID:   1177401
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0446
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   VVUL216895:VV1_0446-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39223; Mature: 39223

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVSDFHFDLPDELIARYPQSERTASRLLQLNGNTGAVKDGSFKDVLELVQAGDLLVFNN
CCCCCCCCCCCHHHHHHCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHCCCEEEECC
TRVIPARMFGRKESGGKLEVLVERMLDEKRFLAHVRSSKSPKPGTLVFLGEEDQYSAEMV
CEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
ARQDALFELHLKADKTILEVLEEIGHMPLPPYIDRPDEDADKERYQTVYNQKPGAVAAPT
HHHHHHEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCC
AGLHFDNQLLEQIKAKGAEFAYVTLHVGAGTFQPVKVDNILEHHMHSEYAEVPQEVVDAI
CCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
NATKARGGRVIAVGTTSVRSLESAAQESLKNGTELMPFFGDTEIFIFPGYQYQLVDCLIT
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCEECEEECCCEEEEECCCHHHHHHHHHH
NFHLPESTLIMLVSAFAGYDHTMNAYQHAVSNQYRFFSYGDAMFIEKKTQ
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCEEEEEECCC
>Mature Secondary Structure
MQVSDFHFDLPDELIARYPQSERTASRLLQLNGNTGAVKDGSFKDVLELVQAGDLLVFNN
CCCCCCCCCCCHHHHHHCCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHCCCEEEECC
TRVIPARMFGRKESGGKLEVLVERMLDEKRFLAHVRSSKSPKPGTLVFLGEEDQYSAEMV
CEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
ARQDALFELHLKADKTILEVLEEIGHMPLPPYIDRPDEDADKERYQTVYNQKPGAVAAPT
HHHHHHEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCC
AGLHFDNQLLEQIKAKGAEFAYVTLHVGAGTFQPVKVDNILEHHMHSEYAEVPQEVVDAI
CCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
NATKARGGRVIAVGTTSVRSLESAAQESLKNGTELMPFFGDTEIFIFPGYQYQLVDCLIT
HHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCEECEEECCCEEEEECCCHHHHHHHHHH
NFHLPESTLIMLVSAFAGYDHTMNAYQHAVSNQYRFFSYGDAMFIEKKTQ
HCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA