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The map label for this gene is ntrC [H]

Identifier: 23501995

GI number: 23501995

Start: 1094500

End: 1095975

Strand: Reverse

Name: ntrC [H]

Synonym: BR1117

Alternate gene names: 23501995

Gene position: 1095975-1094500 (Counterclockwise)

Preceding gene: 23501996

Following gene: 23501994

Centisome position: 52.0

GC content: 59.35

Gene sequence:

>1476_bases
ATGATTGCAGGGCGTCCGACTGGAACTGTACTGGTGGCAGACGACGATGCAGCCATTCGCACGGTGCTCAATCAGGCATT
GTCCCGTGCTGGTTATGATGTGCGGGTGACGTCCAATGCCGCATCGCTCTGGCGCTGGGTTGCGGCGGGCGATGGCGATC
TGGTGATCAGCGATGTCATGATGCCCGATGAAAATGCATTCGATCTCTTGCCGCGCATCAAGAAGATACGCCCCGATCTG
CCCGTTGTGGTCATGAGCGCGCAGAACACATTCATGACCGCAATCCGCGCTTCCGAGGCGGGGGCTTATGAGTATCTGCC
AAAGCCGTTCGATCTGACCGAGCTTATCAATATCGTAGGGCGCGCGCTCTCGGAACCCAGGCAGAAGCCTGCCGTGGCGC
TTCCCGACGAGCAGGGTGAGAACATGCCCCTTGTGGGCCGTTCGCCCGCCATGCAGGAAATCTACCGTCTTCTGGCGCGC
ATGATGCAGACAGACCTCACCGTCATGGTGACGGGTGAATCCGGCACCGGCAAGGAACTCGTCGCGCGTGCCCTGCATGA
ATATGGCCGCCGCCGCAAGGGACCGTTCGTGGCGATCAATATGGCTGCAATCCCGCGTGACCTTATCGAATCGGAACTGT
TCGGGCACGAAAAGGGAGCATTCACCGGCGCACAGAACCGGTCGAGCGGGCGCTTTGAGCAGGCCAATGGCGGTACGCTG
TTTCTGGACGAAATTGGCGATATGCCGATGGAGGCGCAAACGCGCCTTCTGCGCGTGCTGCAACAGGGCGAATATATGAC
CGTTGGCGGGCGCACGCCGATCAAGACCGATGTGCGCATCGTTGCCGCCACCAACAGGGATTTACGACAGATCATCGCGC
AGGGGCTTTTCCGCGAAGACCTCTACTACCGCTTGAATGTAGTGCCGCTGCGCCTGCCGCCGCTTCGCGAACGAAGCGAG
GATGTCCCCGATCTCGTGCGCCATTTCTTCAAACGCGCTGCGGAAGAGGGGTTGCCCGAAAAGCGCATTACCAATGCGGG
TCTGGAGATGATGCGGCGCTATCCGTGGCCGGGCAATGTGCGCGAGCTTGAAAATCTCGTGCGCAGGCTTGCCGCGCTCT
ATCCGCAGGAAGAAATCGCGCCGGAAATCATCGAAACCGAGCTCAAGGCCGAATTGGCCAAGCCGGACGTTGTTGCCGCT
GCTGGCGGCGAGATGGAAAATATCTCCATTTCACAAGCCGTGGAGCAGAACATGCAGCGCTATTTCGCTTCGTTCGGTTC
GGATCTGCCGCCGCCGGGGCTTTATCAGCGTGTTCTGGCTGAAGTGGAATATCCGCTGATCCTTGCCTGCCTGACGGCCA
CCCGCGGCAACCAGATCAAGGCCGCCGATCTTTTAGGACTTAACCGGAATACGTTGCGCAAGAAAATCCGCGAACTCGGC
GTCAATATCTATCGTGGCGCAAAGACCGGCGGTTAG

Upstream 100 bases:

>100_bases
ATCCTGCGCGCACGACTTTCCGTATTCTCATGCCAGCCTGGAAAGGCCCCGGCGAACCGGAAATCAGGTCAAACACTATT
GGCAACGCTGGAGACAATGC

Downstream 100 bases:

>100_bases
CACTACAGTTGCATTCTTCGTGATGCCGGGCTGCAAAGATCTGCTGCCGGGAATGCTTTAATCACAAGCAATCGTTGCAT
TTTCGCCACAATGCGTTGCA

Product: nitrogen regulation protein NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MIAGRPTGTVLVADDDAAIRTVLNQALSRAGYDVRVTSNAASLWRWVAAGDGDLVISDVMMPDENAFDLLPRIKKIRPDL
PVVVMSAQNTFMTAIRASEAGAYEYLPKPFDLTELINIVGRALSEPRQKPAVALPDEQGENMPLVGRSPAMQEIYRLLAR
MMQTDLTVMVTGESGTGKELVARALHEYGRRRKGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSSGRFEQANGGTL
FLDEIGDMPMEAQTRLLRVLQQGEYMTVGGRTPIKTDVRIVAATNRDLRQIIAQGLFREDLYYRLNVVPLRLPPLRERSE
DVPDLVRHFFKRAAEEGLPEKRITNAGLEMMRRYPWPGNVRELENLVRRLAALYPQEEIAPEIIETELKAELAKPDVVAA
AGGEMENISISQAVEQNMQRYFASFGSDLPPPGLYQRVLAEVEYPLILACLTATRGNQIKAADLLGLNRNTLRKKIRELG
VNIYRGAKTGG

Sequences:

>Translated_491_residues
MIAGRPTGTVLVADDDAAIRTVLNQALSRAGYDVRVTSNAASLWRWVAAGDGDLVISDVMMPDENAFDLLPRIKKIRPDL
PVVVMSAQNTFMTAIRASEAGAYEYLPKPFDLTELINIVGRALSEPRQKPAVALPDEQGENMPLVGRSPAMQEIYRLLAR
MMQTDLTVMVTGESGTGKELVARALHEYGRRRKGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSSGRFEQANGGTL
FLDEIGDMPMEAQTRLLRVLQQGEYMTVGGRTPIKTDVRIVAATNRDLRQIIAQGLFREDLYYRLNVVPLRLPPLRERSE
DVPDLVRHFFKRAAEEGLPEKRITNAGLEMMRRYPWPGNVRELENLVRRLAALYPQEEIAPEIIETELKAELAKPDVVAA
AGGEMENISISQAVEQNMQRYFASFGSDLPPPGLYQRVLAEVEYPLILACLTATRGNQIKAADLLGLNRNTLRKKIRELG
VNIYRGAKTGG
>Mature_491_residues
MIAGRPTGTVLVADDDAAIRTVLNQALSRAGYDVRVTSNAASLWRWVAAGDGDLVISDVMMPDENAFDLLPRIKKIRPDL
PVVVMSAQNTFMTAIRASEAGAYEYLPKPFDLTELINIVGRALSEPRQKPAVALPDEQGENMPLVGRSPAMQEIYRLLAR
MMQTDLTVMVTGESGTGKELVARALHEYGRRRKGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSSGRFEQANGGTL
FLDEIGDMPMEAQTRLLRVLQQGEYMTVGGRTPIKTDVRIVAATNRDLRQIIAQGLFREDLYYRLNVVPLRLPPLRERSE
DVPDLVRHFFKRAAEEGLPEKRITNAGLEMMRRYPWPGNVRELENLVRRLAALYPQEEIAPEIIETELKAELAKPDVVAA
AGGEMENISISQAVEQNMQRYFASFGSDLPPPGLYQRVLAEVEYPLILACLTATRGNQIKAADLLGLNRNTLRKKIRELG
VNIYRGAKTGG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=475, Percent_Identity=45.0526315789474, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1788550, Length=481, Percent_Identity=37.2141372141372, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1790437, Length=468, Percent_Identity=40.5982905982906, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1788905, Length=401, Percent_Identity=40.1496259351621, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1789233, Length=391, Percent_Identity=40.153452685422, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=51.9480519480519, Blast_Score=245, Evalue=6e-66,
Organism=Escherichia coli, GI1789087, Length=343, Percent_Identity=41.9825072886297, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI87082152, Length=313, Percent_Identity=41.5335463258786, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786524, Length=271, Percent_Identity=43.9114391143911, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87081872, Length=228, Percent_Identity=46.0526315789474, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1787583, Length=242, Percent_Identity=40.495867768595, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1789828, Length=258, Percent_Identity=35.6589147286822, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI87081858, Length=315, Percent_Identity=28.8888888888889, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1786784, Length=229, Percent_Identity=24.4541484716157, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789809, Length=107, Percent_Identity=30.8411214953271, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789661, Length=71, Percent_Identity=47.887323943662, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790552, Length=137, Percent_Identity=29.9270072992701, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54509; Mature: 54509

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGRPTGTVLVADDDAAIRTVLNQALSRAGYDVRVTSNAASLWRWVAAGDGDLVISDVM
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCEEEEECC
MPDENAFDLLPRIKKIRPDLPVVVMSAQNTFMTAIRASEAGAYEYLPKPFDLTELINIVG
CCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHH
RALSEPRQKPAVALPDEQGENMPLVGRSPAMQEIYRLLARMMQTDLTVMVTGESGTGKEL
HHHHCHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
VARALHEYGRRRKGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSSGRFEQANGGTL
HHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
FLDEIGDMPMEAQTRLLRVLQQGEYMTVGGRTPIKTDVRIVAATNRDLRQIIAQGLFRED
EHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
LYYRLNVVPLRLPPLRERSEDVPDLVRHFFKRAAEEGLPEKRITNAGLEMMRRYPWPGNV
HHEEEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCH
RELENLVRRLAALYPQEEIAPEIIETELKAELAKPDVVAAAGGEMENISISQAVEQNMQR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHH
YFASFGSDLPPPGLYQRVLAEVEYPLILACLTATRGNQIKAADLLGLNRNTLRKKIRELG
HHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHC
VNIYRGAKTGG
CEEECCCCCCC
>Mature Secondary Structure
MIAGRPTGTVLVADDDAAIRTVLNQALSRAGYDVRVTSNAASLWRWVAAGDGDLVISDVM
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCCCEEEEECC
MPDENAFDLLPRIKKIRPDLPVVVMSAQNTFMTAIRASEAGAYEYLPKPFDLTELINIVG
CCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHH
RALSEPRQKPAVALPDEQGENMPLVGRSPAMQEIYRLLARMMQTDLTVMVTGESGTGKEL
HHHHCHHHCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHH
VARALHEYGRRRKGPFVAINMAAIPRDLIESELFGHEKGAFTGAQNRSSGRFEQANGGTL
HHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
FLDEIGDMPMEAQTRLLRVLQQGEYMTVGGRTPIKTDVRIVAATNRDLRQIIAQGLFRED
EHHHHCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
LYYRLNVVPLRLPPLRERSEDVPDLVRHFFKRAAEEGLPEKRITNAGLEMMRRYPWPGNV
HHEEEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCH
RELENLVRRLAALYPQEEIAPEIIETELKAELAKPDVVAAAGGEMENISISQAVEQNMQR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHH
YFASFGSDLPPPGLYQRVLAEVEYPLILACLTATRGNQIKAADLLGLNRNTLRKKIRELG
HHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHHC
VNIYRGAKTGG
CEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]