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The map label for this gene is ntrB [H]

Identifier: 23501996

GI number: 23501996

Start: 1095972

End: 1097075

Strand: Reverse

Name: ntrB [H]

Synonym: BR1118

Alternate gene names: 23501996

Gene position: 1097075-1095972 (Counterclockwise)

Preceding gene: 23501997

Following gene: 23501995

Centisome position: 52.05

GC content: 59.78

Gene sequence:

>1104_bases
GTGCTTGATGCGATCAGCCAGCCGGTCATCATGGTGAGTGCGGATAATCACATCGTCTATGCCAATCTGGATGCGGAGCA
GTTTTTCCTGTCCGGCTCATCCATTCTGGCGCGAAACCGGCTGGAATCCTTTCTTCCCTTCGGAAGCCCCCTGCTTGCGC
TGGTCGATCAGGTGCGGGCGAGCCAGATGCCGGTCAACGAATATCGTGTCGATATTTCTTCTCCGCGTCTGGGCGCCGAA
CGGATCGTGGATATTTATGTTGCACCGGTGGCGGAAATGCCGGGAGCGGTGGTCATTCTCTTCAAGGAAAAGAGCATGGC
GGAAAAGATCGACCGCCAGATGACGCATCGCGGTGCGGCGCGTTCAGTTACCGGCCTTGCATCCATGCTGGCGCACGAGA
TCAAGAACCCGCTTTCCGGTATTCGCGGTGCGGCGCAGCTTCTGGAACAGGTGGTCAGCGATGAAGATCGCGCGCTGGCG
CGGCTTATCACCGAGGAGACCGACCGTATTGTCTCGCTTGTGGACCGGATGGAGGTTTTTTCCGACGAGCGCCCCATCGA
GCGCACGGCGGTCAATATCCATGCCGTGCTGGACCATGTGAAGGCCATTGCGCGCAATGGTTTCGCACGGAAAATCCGCT
TCAGCGAGGATTACGATCCATCCCTGCCGCCGGTCTATGCCAATCGCGACCAGCTTGTGCAGGTGTTTCTCAATCTGGTG
AAAAATGCCTGCGAGGCGATGGGTGGCCGCGATGGCGGCGAGATCGTGCTTTCGACAGCCTATCGCCCCGGCATTCGCTT
GCAGGTGCCGGGCGCGCGCGACCGCGTGGCGCTGCCGCTGGAGTTCTGCGTGCATGATAACGGGCCGGGCGTGCCGCAGG
ACATGCTGCCGCACCTCTTCGACCCATTCGTAACCACAAAAACCAACGGCTCCGGGCTGGGGCTGGCGCTGGTTGCCAAG
ATCATCGGCGACCATGGCGGCATCATCGAATGCGACAGCCATCCTGCGCGCACGACTTTCCGTATTCTCATGCCAGCCTG
GAAAGGCCCCGGCGAACCGGAAATCAGGTCAAACACTATTGGCAACGCTGGAGACAATGCATGA

Upstream 100 bases:

>100_bases
TGGTTGAAACCCTCCGGCGAATATTCCTGCGCGATGTCGATCCGCTGCGAAAGGTTGCCTGATGCGCAATAAGACTGAAA
CGGGCGGCGTTCCTTCCATC

Downstream 100 bases:

>100_bases
TTGCAGGGCGTCCGACTGGAACTGTACTGGTGGCAGACGACGATGCAGCCATTCGCACGGTGCTCAATCAGGCATTGTCC
CGTGCTGGTTATGATGTGCG

Product: nitrogen regulation protein NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MLDAISQPVIMVSADNHIVYANLDAEQFFLSGSSILARNRLESFLPFGSPLLALVDQVRASQMPVNEYRVDISSPRLGAE
RIVDIYVAPVAEMPGAVVILFKEKSMAEKIDRQMTHRGAARSVTGLASMLAHEIKNPLSGIRGAAQLLEQVVSDEDRALA
RLITEETDRIVSLVDRMEVFSDERPIERTAVNIHAVLDHVKAIARNGFARKIRFSEDYDPSLPPVYANRDQLVQVFLNLV
KNACEAMGGRDGGEIVLSTAYRPGIRLQVPGARDRVALPLEFCVHDNGPGVPQDMLPHLFDPFVTTKTNGSGLGLALVAK
IIGDHGGIIECDSHPARTTFRILMPAWKGPGEPEIRSNTIGNAGDNA

Sequences:

>Translated_367_residues
MLDAISQPVIMVSADNHIVYANLDAEQFFLSGSSILARNRLESFLPFGSPLLALVDQVRASQMPVNEYRVDISSPRLGAE
RIVDIYVAPVAEMPGAVVILFKEKSMAEKIDRQMTHRGAARSVTGLASMLAHEIKNPLSGIRGAAQLLEQVVSDEDRALA
RLITEETDRIVSLVDRMEVFSDERPIERTAVNIHAVLDHVKAIARNGFARKIRFSEDYDPSLPPVYANRDQLVQVFLNLV
KNACEAMGGRDGGEIVLSTAYRPGIRLQVPGARDRVALPLEFCVHDNGPGVPQDMLPHLFDPFVTTKTNGSGLGLALVAK
IIGDHGGIIECDSHPARTTFRILMPAWKGPGEPEIRSNTIGNAGDNA
>Mature_367_residues
MLDAISQPVIMVSADNHIVYANLDAEQFFLSGSSILARNRLESFLPFGSPLLALVDQVRASQMPVNEYRVDISSPRLGAE
RIVDIYVAPVAEMPGAVVILFKEKSMAEKIDRQMTHRGAARSVTGLASMLAHEIKNPLSGIRGAAQLLEQVVSDEDRALA
RLITEETDRIVSLVDRMEVFSDERPIERTAVNIHAVLDHVKAIARNGFARKIRFSEDYDPSLPPVYANRDQLVQVFLNLV
KNACEAMGGRDGGEIVLSTAYRPGIRLQVPGARDRVALPLEFCVHDNGPGVPQDMLPHLFDPFVTTKTNGSGLGLALVAK
IIGDHGGIIECDSHPARTTFRILMPAWKGPGEPEIRSNTIGNAGDNA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=352, Percent_Identity=32.3863636363636, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1788549, Length=285, Percent_Identity=30.1754385964912, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1790436, Length=224, Percent_Identity=30.8035714285714, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1790567, Length=130, Percent_Identity=36.1538461538462, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789808, Length=220, Percent_Identity=27.7272727272727, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788393, Length=219, Percent_Identity=27.3972602739726, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=28.3842794759825, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=28.4552845528455, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=25.5813953488372, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40044; Mature: 40044

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAISQPVIMVSADNHIVYANLDAEQFFLSGSSILARNRLESFLPFGSPLLALVDQVRA
CCCCCCCCEEEEECCCEEEEEECCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
SQMPVNEYRVDISSPRLGAERIVDIYVAPVAEMPGAVVILFKEKSMAEKIDRQMTHRGAA
HCCCHHHHEEECCCCCCCHHHHHHHHEECHHHCCCEEEEEECCHHHHHHHHHHHHHCCHH
RSVTGLASMLAHEIKNPLSGIRGAAQLLEQVVSDEDRALARLITEETDRIVSLVDRMEVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SDERPIERTAVNIHAVLDHVKAIARNGFARKIRFSEDYDPSLPPVYANRDQLVQVFLNLV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
KNACEAMGGRDGGEIVLSTAYRPGIRLQVPGARDRVALPLEFCVHDNGPGVPQDMLPHLF
HHHHHHCCCCCCCCEEEEECCCCCCEEECCCCCCCEECEEEEEEECCCCCCCHHHHHHHH
DPFVTTKTNGSGLGLALVAKIIGDHGGIIECDSHPARTTFRILMPAWKGPGEPEIRSNTI
CCCCEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCC
GNAGDNA
CCCCCCC
>Mature Secondary Structure
MLDAISQPVIMVSADNHIVYANLDAEQFFLSGSSILARNRLESFLPFGSPLLALVDQVRA
CCCCCCCCEEEEECCCEEEEEECCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
SQMPVNEYRVDISSPRLGAERIVDIYVAPVAEMPGAVVILFKEKSMAEKIDRQMTHRGAA
HCCCHHHHEEECCCCCCCHHHHHHHHEECHHHCCCEEEEEECCHHHHHHHHHHHHHCCHH
RSVTGLASMLAHEIKNPLSGIRGAAQLLEQVVSDEDRALARLITEETDRIVSLVDRMEVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SDERPIERTAVNIHAVLDHVKAIARNGFARKIRFSEDYDPSLPPVYANRDQLVQVFLNLV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
KNACEAMGGRDGGEIVLSTAYRPGIRLQVPGARDRVALPLEFCVHDNGPGVPQDMLPHLF
HHHHHHCCCCCCCCEEEEECCCCCCEEECCCCCCCEECEEEEEEECCCCCCCHHHHHHHH
DPFVTTKTNGSGLGLALVAKIIGDHGGIIECDSHPARTTFRILMPAWKGPGEPEIRSNTI
CCCCEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCC
GNAGDNA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 2881918 [H]