Click here to switch to the map view.

The map label for this gene is pucL [H]

Identifier: 23501252

GI number: 23501252

Start: 356177

End: 357604

Strand: Reverse

Name: pucL [H]

Synonym: BR0347

Alternate gene names: 23501252

Gene position: 357604-356177 (Counterclockwise)

Preceding gene: 23501257

Following gene: 23501251

Centisome position: 16.97

GC content: 61.2

Gene sequence:

>1428_bases
ATGAAATATCCGCGCAATCTCGTCGGCTATGGACGCAACACGCCAGACCCGCGCTGGCCGGGAGGGGCAAATATCGCCGT
GCAGTTTGTCGTCAATTATGAGGAAGGCGGCGAATGCTGCATTCTCGATGGCGACCCGGCTTCCGAATGCCTGCTTTCCG
AGATTGTCGGCGCGCAGCCCTGGCGGGGCCAGCGCAATCTCAACATGGAATCCATTTATGAATATGGCGCCCGTTCCGGC
TTCTGGCGCCTGTGGCGCGCCTTTACCCGCCGCAATATGCCCGTCACCGTCTATGGGGTGACGCTTGCCATGGCGCGCAA
CCCGGAAGCCGTGGCCGCCATGAAAGAAGCCGGCTGGGAAATCGCCAGCCATGGCCTGCGATGGTGGGAATACAAAGACG
TGCCGGAGGAGATCGAGCGCGAACATATCCGCGAGGCCGTGCGCCTCCACACGGAAGTTACCGGCTCGCGCCCGCTCGGC
ATCTATCAGGGCAAGCCCTCCGACAACACGCTGAAGCTGGTGATGGAGGAAGGCGGCTTCCTCTATTCCGCCGATTCCTA
TGCCGACGAACTGCCCTATTGGGTAAAGGGCCCGAAAGGCCCGCACCTGATCGTGCCCTATACGCTGGACGCAAACGACA
TGCGTTTCGCCACGCCACAAGGCTTTAATTCCGGCGACCAGTTCTTCACCTATCTGAAAGACAGTTTCGACGTTCTCTAT
CGCGAGGGTGAGGAAGGCGCGCCCAAGATGATGTCCATCGGCCTGCATTGCCGTCTGGCGGGGCGCCCCGGCCGCATGGC
GGCGCTGGAACGTTTCCTCGATTACGTGCAGAGCCACGACAAAGTCTGGGTGGCCAAACGTGTGGATATCGCCCGCCACT
GGCATAAACACCACCAGCCGGTCGTGGCCGATGTGGTGCCTTCCAAAGCGGACAGGCAGGCCTTTGTCGCGCGTTATGGC
AGCATTTTCGAGCATTCGCCCTGGATTGCCGAGCGCGCCTATGATGCCCGGCTCACTGTGGAAGAAGACAGTGCTTCGGG
CCTTGCCGCCGCGATGACCAAGGTGTTCCGCACCGCAGCGCCGGATGAACGGCTGGCCGTTTTGAAAGCGCATCCCGACC
TTGCGGGCAAGCTGGCGCAGGCCAGGCGACTGACGGCGGAATCAACCGCCGAACAGGCATCGGCCGGGCTGGACGCGCTG
ACGGACACCGAAAAGCAGGCCTTTACCGAATTGAACGACGCCTATACCGCCAAGTTCGGCTTTCCCTTCATCATTGCGGT
CAAGGGCCGCAACAAGCAGGAGATACTCGACGCCTTCAGGCGGCGCGTTTCCAACGACCGTGATACGGAGTTTGAAACCG
CCTGCGCGCAGGTGGAACGCATCGCGCTCCTGCGCCTGAAAGACATCCTCCCCGAAACACTCTATTGA

Upstream 100 bases:

>100_bases
ATGGTTATCCTTGGATGTCTTTCAAATTTTTAGGGGGGGAATAGCCATGATAAATCTTTATTATCATGGGCAAAACAAGT
GAGAATGAGGGAAGACCCGC

Downstream 100 bases:

>100_bases
TCGGATCCAGTCATGAGCCGCAATTATTATGCACCAACGGGCGGCCTGCCGCCGCAAACCCAGCTTCTCACCGACCGCGC
CATGTTTACCGAAGCCTATG

Product: hypothetical protein

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKYPRNLVGYGRNTPDPRWPGGANIAVQFVVNYEEGGECCILDGDPASECLLSEIVGAQPWRGQRNLNMESIYEYGARSG
FWRLWRAFTRRNMPVTVYGVTLAMARNPEAVAAMKEAGWEIASHGLRWWEYKDVPEEIEREHIREAVRLHTEVTGSRPLG
IYQGKPSDNTLKLVMEEGGFLYSADSYADELPYWVKGPKGPHLIVPYTLDANDMRFATPQGFNSGDQFFTYLKDSFDVLY
REGEEGAPKMMSIGLHCRLAGRPGRMAALERFLDYVQSHDKVWVAKRVDIARHWHKHHQPVVADVVPSKADRQAFVARYG
SIFEHSPWIAERAYDARLTVEEDSASGLAAAMTKVFRTAAPDERLAVLKAHPDLAGKLAQARRLTAESTAEQASAGLDAL
TDTEKQAFTELNDAYTAKFGFPFIIAVKGRNKQEILDAFRRRVSNDRDTEFETACAQVERIALLRLKDILPETLY

Sequences:

>Translated_475_residues
MKYPRNLVGYGRNTPDPRWPGGANIAVQFVVNYEEGGECCILDGDPASECLLSEIVGAQPWRGQRNLNMESIYEYGARSG
FWRLWRAFTRRNMPVTVYGVTLAMARNPEAVAAMKEAGWEIASHGLRWWEYKDVPEEIEREHIREAVRLHTEVTGSRPLG
IYQGKPSDNTLKLVMEEGGFLYSADSYADELPYWVKGPKGPHLIVPYTLDANDMRFATPQGFNSGDQFFTYLKDSFDVLY
REGEEGAPKMMSIGLHCRLAGRPGRMAALERFLDYVQSHDKVWVAKRVDIARHWHKHHQPVVADVVPSKADRQAFVARYG
SIFEHSPWIAERAYDARLTVEEDSASGLAAAMTKVFRTAAPDERLAVLKAHPDLAGKLAQARRLTAESTAEQASAGLDAL
TDTEKQAFTELNDAYTAKFGFPFIIAVKGRNKQEILDAFRRRVSNDRDTEFETACAQVERIALLRLKDILPETLY
>Mature_475_residues
MKYPRNLVGYGRNTPDPRWPGGANIAVQFVVNYEEGGECCILDGDPASECLLSEIVGAQPWRGQRNLNMESIYEYGARSG
FWRLWRAFTRRNMPVTVYGVTLAMARNPEAVAAMKEAGWEIASHGLRWWEYKDVPEEIEREHIREAVRLHTEVTGSRPLG
IYQGKPSDNTLKLVMEEGGFLYSADSYADELPYWVKGPKGPHLIVPYTLDANDMRFATPQGFNSGDQFFTYLKDSFDVLY
REGEEGAPKMMSIGLHCRLAGRPGRMAALERFLDYVQSHDKVWVAKRVDIARHWHKHHQPVVADVVPSKADRQAFVARYG
SIFEHSPWIAERAYDARLTVEEDSASGLAAAMTKVFRTAAPDERLAVLKAHPDLAGKLAQARRLTAESTAEQASAGLDAL
TDTEKQAFTELNDAYTAKFGFPFIIAVKGRNKQEILDAFRRRVSNDRDTEFETACAQVERIALLRLKDILPETLY

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=157, Percent_Identity=34.3949044585987, Blast_Score=81, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 53611; Mature: 53611

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYPRNLVGYGRNTPDPRWPGGANIAVQFVVNYEEGGECCILDGDPASECLLSEIVGAQP
CCCCCCHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHCCCC
WRGQRNLNMESIYEYGARSGFWRLWRAFTRRNMPVTVYGVTLAMARNPEAVAAMKEAGWE
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHCHH
IASHGLRWWEYKDVPEEIEREHIREAVRLHTEVTGSRPLGIYQGKPSDNTLKLVMEEGGF
HHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCE
LYSADSYADELPYWVKGPKGPHLIVPYTLDANDMRFATPQGFNSGDQFFTYLKDSFDVLY
EEECCCHHHHCCCEEECCCCCEEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHH
REGEEGAPKMMSIGLHCRLAGRPGRMAALERFLDYVQSHDKVWVAKRVDIARHWHKHHQP
HCCCCCCCHHEEECEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCC
VVADVVPSKADRQAFVARYGSIFEHSPWIAERAYDARLTVEEDSASGLAAAMTKVFRTAA
EEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCC
PDERLAVLKAHPDLAGKLAQARRLTAESTAEQASAGLDALTDTEKQAFTELNDAYTAKFG
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHEEEECC
FPFIIAVKGRNKQEILDAFRRRVSNDRDTEFETACAQVERIALLRLKDILPETLY
CCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYPRNLVGYGRNTPDPRWPGGANIAVQFVVNYEEGGECCILDGDPASECLLSEIVGAQP
CCCCCCHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHCCCC
WRGQRNLNMESIYEYGARSGFWRLWRAFTRRNMPVTVYGVTLAMARNPEAVAAMKEAGWE
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHCHH
IASHGLRWWEYKDVPEEIEREHIREAVRLHTEVTGSRPLGIYQGKPSDNTLKLVMEEGGF
HHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCE
LYSADSYADELPYWVKGPKGPHLIVPYTLDANDMRFATPQGFNSGDQFFTYLKDSFDVLY
EEECCCHHHHCCCEEECCCCCEEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHH
REGEEGAPKMMSIGLHCRLAGRPGRMAALERFLDYVQSHDKVWVAKRVDIARHWHKHHQP
HCCCCCCCHHEEECEEEEECCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCC
VVADVVPSKADRQAFVARYGSIFEHSPWIAERAYDARLTVEEDSASGLAAAMTKVFRTAA
EEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCC
PDERLAVLKAHPDLAGKLAQARRLTAESTAEQASAGLDALTDTEKQAFTELNDAYTAKFG
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHEEEECC
FPFIIAVKGRNKQEILDAFRRRVSNDRDTEFETACAQVERIALLRLKDILPETLY
CCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]