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The map label for this gene is dmlR [H]

Identifier: 23501257

GI number: 23501257

Start: 362944

End: 363858

Strand: Reverse

Name: dmlR [H]

Synonym: BR0352

Alternate gene names: 23501257

Gene position: 363858-362944 (Counterclockwise)

Preceding gene: 23501261

Following gene: 23501252

Centisome position: 17.26

GC content: 58.03

Gene sequence:

>915_bases
GTGTCATATCTTGATAATCTGCGCGTTTTCGTGCGCGTTGTCGAATTGGGAAATCTCTCGGCGGCAGGCCGCGACCAACG
CGCCTCGCCTGCGGTAGTAAGCAATCGAATCAAGGAGTTGGAGAAGAATCTGGGGGTGCGGCTCTTCAACCGGACCACGC
GCAAGCTGACGCCGACGGAGCATGGCCGCGTATTTTATGATGGCGCATTGAAAATCCTTGAAGCCGTTGATGAGGCAGAA
GCCGCAGTGGCGGAACTGGCCAAAAACCCGAAGGGGTCGATTCGCATCACGGCTCCGCTCGGCTTCGGCCGGAGGCTCAT
CGCGTCGGGTATTCCCGAATTTCACGACAGGTATCCCGACATTGAAGTGCGGCTTCGCCTCTCCGATCACGAGGTCGATA
TCATGAGCGAGGGCGTGGATGTCGCCTTCAAGCTCGGCGTGCTGGAAAACTCGAACCTGCGCATGCGCGGCATCATGAAT
TGCGAGCGCGTGGTGTGTGCCGCGCCGCAATATCTTGAAAGGCACGGCACGCCACAATCTCCCGAGGCGCTTTTGAGCGA
CAGGCATCATTGCCTTTTGCTCCGCTTTCCCGGTTCCAAGGAATATTATTGGCTGTTGCAAACCCCCGAGGGCTTGCGCA
AATTCGAGGTTACCGGGCCTTATGATTCCGATGATGGCGATGTGTTGACGCAATGGGCGCTCGGCGGGCGCGGCATCATC
AACAAGCCGCTGTTTGAAGTGAAGGAATATATCCGCGACGGGCGGCTGACGCCCATCCTCCAACACACGCCGCCTGCACC
GATCCAGCTTGCCGCAATCTATCCGCACAAGCGACTTCAGGATCCCAAAGTGCGCCTTATCATCGATTTCATGACAGAGC
GTTGCCAGCGGCTGATCCGCGAGGCGCTTGCCTGA

Upstream 100 bases:

>100_bases
TACTGGAAATGCTTTATATGTGCCAAAGCTTTGCGCTTCTGGGAACGTCACAACACGACCATCACTTTCAAAAAAATCTA
GACAATCATGGGGGAGATGG

Downstream 100 bases:

>100_bases
AAGCCCTCCGGAACCGCTCTAATCCTTTATTTTTACGCGCATCTTTTCCGAAAACCGTTTCACACTTTTCGGGATGCGCT
CTAAACTAGAACCGCTGTCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSYLDNLRVFVRVVELGNLSAAGRDQRASPAVVSNRIKELEKNLGVRLFNRTTRKLTPTEHGRVFYDGALKILEAVDEAE
AAVAELAKNPKGSIRITAPLGFGRRLIASGIPEFHDRYPDIEVRLRLSDHEVDIMSEGVDVAFKLGVLENSNLRMRGIMN
CERVVCAAPQYLERHGTPQSPEALLSDRHHCLLLRFPGSKEYYWLLQTPEGLRKFEVTGPYDSDDGDVLTQWALGGRGII
NKPLFEVKEYIRDGRLTPILQHTPPAPIQLAAIYPHKRLQDPKVRLIIDFMTERCQRLIREALA

Sequences:

>Translated_304_residues
MSYLDNLRVFVRVVELGNLSAAGRDQRASPAVVSNRIKELEKNLGVRLFNRTTRKLTPTEHGRVFYDGALKILEAVDEAE
AAVAELAKNPKGSIRITAPLGFGRRLIASGIPEFHDRYPDIEVRLRLSDHEVDIMSEGVDVAFKLGVLENSNLRMRGIMN
CERVVCAAPQYLERHGTPQSPEALLSDRHHCLLLRFPGSKEYYWLLQTPEGLRKFEVTGPYDSDDGDVLTQWALGGRGII
NKPLFEVKEYIRDGRLTPILQHTPPAPIQLAAIYPHKRLQDPKVRLIIDFMTERCQRLIREALA
>Mature_303_residues
SYLDNLRVFVRVVELGNLSAAGRDQRASPAVVSNRIKELEKNLGVRLFNRTTRKLTPTEHGRVFYDGALKILEAVDEAEA
AVAELAKNPKGSIRITAPLGFGRRLIASGIPEFHDRYPDIEVRLRLSDHEVDIMSEGVDVAFKLGVLENSNLRMRGIMNC
ERVVCAAPQYLERHGTPQSPEALLSDRHHCLLLRFPGSKEYYWLLQTPEGLRKFEVTGPYDSDDGDVLTQWALGGRGIIN
KPLFEVKEYIRDGRLTPILQHTPPAPIQLAAIYPHKRLQDPKVRLIIDFMTERCQRLIREALA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=293, Percent_Identity=33.1058020477816, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=30.1369863013699, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=31.3993174061433, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1789639, Length=294, Percent_Identity=30.2721088435374, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=29.5918367346939, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1787128, Length=299, Percent_Identity=29.7658862876254, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1787589, Length=285, Percent_Identity=24.9122807017544, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1790011, Length=317, Percent_Identity=25.5520504731861, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788481, Length=170, Percent_Identity=30.5882352941176, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=27.4509803921569, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=122, Percent_Identity=31.1475409836066, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788706, Length=193, Percent_Identity=24.3523316062176, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788748, Length=126, Percent_Identity=34.1269841269841, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=131, Percent_Identity=29.0076335877863, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34398; Mature: 34267

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLDNLRVFVRVVELGNLSAAGRDQRASPAVVSNRIKELEKNLGVRLFNRTTRKLTPTE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
HGRVFYDGALKILEAVDEAEAAVAELAKNPKGSIRITAPLGFGRRLIASGIPEFHDRYPD
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCCHHHHCCCC
IEVRLRLSDHEVDIMSEGVDVAFKLGVLENSNLRMRGIMNCERVVCAAPQYLERHGTPQS
EEEEEEECCCCEEHHHCCCCEEEEEEEEECCCEEEEECCCHHHHHHCCHHHHHHCCCCCC
PEALLSDRHHCLLLRFPGSKEYYWLLQTPEGLRKFEVTGPYDSDDGDVLTQWALGGRGII
CHHHHCCCCCEEEEECCCCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEHHHCCCCCCC
NKPLFEVKEYIRDGRLTPILQHTPPAPIQLAAIYPHKRLQDPKVRLIIDFMTERCQRLIR
CCCHHHHHHHHHCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHH
EALA
HHCC
>Mature Secondary Structure 
SYLDNLRVFVRVVELGNLSAAGRDQRASPAVVSNRIKELEKNLGVRLFNRTTRKLTPTE
CHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
HGRVFYDGALKILEAVDEAEAAVAELAKNPKGSIRITAPLGFGRRLIASGIPEFHDRYPD
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCCHHHHCCCC
IEVRLRLSDHEVDIMSEGVDVAFKLGVLENSNLRMRGIMNCERVVCAAPQYLERHGTPQS
EEEEEEECCCCEEHHHCCCCEEEEEEEEECCCEEEEECCCHHHHHHCCHHHHHHCCCCCC
PEALLSDRHHCLLLRFPGSKEYYWLLQTPEGLRKFEVTGPYDSDDGDVLTQWALGGRGII
CHHHHCCCCCEEEEECCCCCCEEEEEECCCCCEEEEECCCCCCCCCCEEEHHHCCCCCCC
NKPLFEVKEYIRDGRLTPILQHTPPAPIQLAAIYPHKRLQDPKVRLIIDFMTERCQRLIR
CCCHHHHHHHHHCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHH
EALA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]