Click here to switch to the map view.

The map label for this gene is ghrB [H]

Identifier: 23100303

GI number: 23100303

Start: 2931669

End: 2932643

Strand: Reverse

Name: ghrB [H]

Synonym: OB2848

Alternate gene names: 23100303

Gene position: 2932643-2931669 (Counterclockwise)

Preceding gene: 23100304

Following gene: 23100302

Centisome position: 80.78

GC content: 36.82

Gene sequence:

>975_bases
ATGAAACGAGAAATTATTTTATATAAATCTATCTCAACAGATTTACTAGAACAATTGAAAAATCGCTTTATCGTTCATCA
GCATCAACTAAAATCAGAAATGGATGATTCCTTCTTTAGTGATTTACAGCGGGTAGAGGGGATTATTGGTTCAAAGTTAC
GAGTTGATGGGCATCTCTTGGATCAAGCACCTCATCTAAAAATTGTCACCAATATATCGGTTGGATATGATAATTTAGAG
ATAGAAGAGCTAACAAAAAGGGGTATTATGGCAACGAATACACCAGATGTTTTAACAGATACAGTAGCTGATACGGTATT
TGGGTTGTTGTTAGCAACATCTAGGAGGATTTGTGAGTTGGATCAATATGTGAAGTTAGGCAGATGGGATGAAAATATTG
GTGAGCATTTATTTGGAGTTGATGTTCATCATAAAACACTGGGTATTATTGGTATGGGGAGAATTGGTTTAGCAGTTGCA
GAAAGGGCACATTACGGATTTAAGATGAAGATTGTTTATCATAATCGTTCCACTCATTCATATGCAGAAAAAAATATCAA
CGCTACTTATGCTTCCTTAGAAGAACTTTTAACAGTTTCCGATTTTGTACTCGTTATGGCACCTTTAGTTCCTGAAACAG
TAAAATTAATCGGGGAGAAGGAATTTGATTTGATGAAAGAAACAGCCATATTTCTAAATGGATCTCGCGGAGAACTTGTC
GATGAGTTGGCACTTATTCATGCTTTACAATCGAAAAAAATTTGGGGAGCAGGTTTAGATGTCTATGAACAAGAGCCGAT
TTCAAAGGAAAGCCTGCTCCTTCAATTGAAGAATGTTGTTACGTTACCGCATATAGGTTCTGCAACGAGGGAAACAAGGT
ATAAGATGGCTAAACTTGCTGTAGATAATCTTATGAAAGGATTCACGGGGGAAGTGCCACCAAGCCTGATCAATCCAGAC
GTTTTAACAGACTAA

Upstream 100 bases:

>100_bases
CGTTGTGTTCTCCATTACAAGGTTAGGATATTCATATTGTAGTTCCACCAAATCTGATATATCATATACCAAAAAGAAAG
ATTTTATAGGAGGACTTGCT

Downstream 100 bases:

>100_bases
TAATCGAAAAAGGAGAAAGATGAATATGGATAAAGACATTCAAAAAAAAATCAGTATGCAATCGCGTTCTGTGCGTGATC
TTATTTATGATTATTTAAAA

Product: 2-ketogluconate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKREIILYKSISTDLLEQLKNRFIVHQHQLKSEMDDSFFSDLQRVEGIIGSKLRVDGHLLDQAPHLKIVTNISVGYDNLE
IEELTKRGIMATNTPDVLTDTVADTVFGLLLATSRRICELDQYVKLGRWDENIGEHLFGVDVHHKTLGIIGMGRIGLAVA
ERAHYGFKMKIVYHNRSTHSYAEKNINATYASLEELLTVSDFVLVMAPLVPETVKLIGEKEFDLMKETAIFLNGSRGELV
DELALIHALQSKKIWGAGLDVYEQEPISKESLLLQLKNVVTLPHIGSATRETRYKMAKLAVDNLMKGFTGEVPPSLINPD
VLTD

Sequences:

>Translated_324_residues
MKREIILYKSISTDLLEQLKNRFIVHQHQLKSEMDDSFFSDLQRVEGIIGSKLRVDGHLLDQAPHLKIVTNISVGYDNLE
IEELTKRGIMATNTPDVLTDTVADTVFGLLLATSRRICELDQYVKLGRWDENIGEHLFGVDVHHKTLGIIGMGRIGLAVA
ERAHYGFKMKIVYHNRSTHSYAEKNINATYASLEELLTVSDFVLVMAPLVPETVKLIGEKEFDLMKETAIFLNGSRGELV
DELALIHALQSKKIWGAGLDVYEQEPISKESLLLQLKNVVTLPHIGSATRETRYKMAKLAVDNLMKGFTGEVPPSLINPD
VLTD
>Mature_324_residues
MKREIILYKSISTDLLEQLKNRFIVHQHQLKSEMDDSFFSDLQRVEGIIGSKLRVDGHLLDQAPHLKIVTNISVGYDNLE
IEELTKRGIMATNTPDVLTDTVADTVFGLLLATSRRICELDQYVKLGRWDENIGEHLFGVDVHHKTLGIIGMGRIGLAVA
ERAHYGFKMKIVYHNRSTHSYAEKNINATYASLEELLTVSDFVLVMAPLVPETVKLIGEKEFDLMKETAIFLNGSRGELV
DELALIHALQSKKIWGAGLDVYEQEPISKESLLLQLKNVVTLPHIGSATRETRYKMAKLAVDNLMKGFTGEVPPSLINPD
VLTD

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=262, Percent_Identity=44.2748091603053, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI23308577, Length=302, Percent_Identity=30.1324503311258, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI145580578, Length=259, Percent_Identity=31.6602316602317, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI4557499, Length=259, Percent_Identity=31.6602316602317, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI145580575, Length=265, Percent_Identity=31.3207547169811, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI61743967, Length=267, Percent_Identity=32.5842696629214, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI4557497, Length=267, Percent_Identity=32.5842696629214, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI87082289, Length=322, Percent_Identity=50, Blast_Score=325, Evalue=2e-90,
Organism=Escherichia coli, GI1789279, Length=317, Percent_Identity=30.2839116719243, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787645, Length=264, Percent_Identity=28.4090909090909, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081824, Length=145, Percent_Identity=32.4137931034483, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17532191, Length=305, Percent_Identity=27.5409836065574, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=265, Percent_Identity=26.7924528301887, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324055, Length=260, Percent_Identity=38.4615384615385, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6320925, Length=257, Percent_Identity=31.5175097276265, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=302, Percent_Identity=28.8079470198675, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321253, Length=283, Percent_Identity=26.5017667844523, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324964, Length=242, Percent_Identity=26.0330578512397, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325144, Length=171, Percent_Identity=30.4093567251462, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28574286, Length=277, Percent_Identity=40.4332129963899, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45551003, Length=274, Percent_Identity=37.956204379562, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI45552429, Length=274, Percent_Identity=37.956204379562, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI28574284, Length=274, Percent_Identity=37.956204379562, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24585514, Length=274, Percent_Identity=37.956204379562, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI28574282, Length=274, Percent_Identity=37.956204379562, Blast_Score=181, Evalue=8e-46,
Organism=Drosophila melanogaster, GI28571528, Length=259, Percent_Identity=40.5405405405405, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24585516, Length=312, Percent_Identity=32.6923076923077, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI19921140, Length=299, Percent_Identity=26.7558528428094, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24646446, Length=265, Percent_Identity=29.811320754717, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24646448, Length=265, Percent_Identity=29.811320754717, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24646452, Length=265, Percent_Identity=29.811320754717, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24646450, Length=265, Percent_Identity=29.811320754717, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI62472511, Length=259, Percent_Identity=30.1158301158301, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 36378; Mature: 36378

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREIILYKSISTDLLEQLKNRFIVHQHQLKSEMDDSFFSDLQRVEGIIGSKLRVDGHLL
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCEEC
DQAPHLKIVTNISVGYDNLEIEELTKRGIMATNTPDVLTDTVADTVFGLLLATSRRICEL
CCCCCEEEEEEEEECCCCCHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCHHHHHH
DQYVKLGRWDENIGEHLFGVDVHHKTLGIIGMGRIGLAVAERAHYGFKMKIVYHNRSTHS
HHHHHHCCCCHHHHHHHCCCEECHHHCCEEEECHHHHHHHHHHCCCEEEEEEEECCCCHH
YAEKNINATYASLEELLTVSDFVLVMAPLVPETVKLIGEKEFDLMKETAIFLNGSRGELV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHEEEECCCHHHH
DELALIHALQSKKIWGAGLDVYEQEPISKESLLLQLKNVVTLPHIGSATRETRYKMAKLA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHH
VDNLMKGFTGEVPPSLINPDVLTD
HHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKREIILYKSISTDLLEQLKNRFIVHQHQLKSEMDDSFFSDLQRVEGIIGSKLRVDGHLL
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCEEC
DQAPHLKIVTNISVGYDNLEIEELTKRGIMATNTPDVLTDTVADTVFGLLLATSRRICEL
CCCCCEEEEEEEEECCCCCHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCHHHHHH
DQYVKLGRWDENIGEHLFGVDVHHKTLGIIGMGRIGLAVAERAHYGFKMKIVYHNRSTHS
HHHHHHCCCCHHHHHHHCCCEECHHHCCEEEECHHHHHHHHHHCCCEEEEEEEECCCCHH
YAEKNINATYASLEELLTVSDFVLVMAPLVPETVKLIGEKEFDLMKETAIFLNGSRGELV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHEEEECCCHHHH
DELALIHALQSKKIWGAGLDVYEQEPISKESLLLQLKNVVTLPHIGSATRETRYKMAKLA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHH
VDNLMKGFTGEVPPSLINPDVLTD
HHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA