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The map label for this gene is aprX [H]

Identifier: 23100304

GI number: 23100304

Start: 2932880

End: 2934187

Strand: Reverse

Name: aprX [H]

Synonym: OB2849

Alternate gene names: 23100304

Gene position: 2934187-2932880 (Counterclockwise)

Preceding gene: 23100305

Following gene: 23100303

Centisome position: 80.82

GC content: 41.9

Gene sequence:

>1308_bases
ATGAGCAACAGGAAAAAAGTATGGTTTGAAGATGCACAACGTAAACTTGACCCTGGCTTGGTGGAACAATTACGGAATTC
TCGAAAAGTAGACCAGAATGAAACTATCAACAATGAAATCCCTCTTATCGTTTATATGAAAAAAGGCTGTGAACGAGGCA
AAAAAGACGACCTCTTAAAGGCATGTAATATTGACTCTCATAACAAACTCCATAGAGAATTGCGCAGTATAAATGCGATG
AAAGGTTATCTGACACCTGATAGAATACAACAGATTAAAGACCATGAAGCAGTTGACCGGATTTTTTACGATCGAATTGT
GACTGCATTCTTAGATATTGCTAGCGAGCAAACAGGAGCTTTCAAAGTTCAGGAACAGCTTGGATTAACTGGAAAAGACG
TTACGATTGCCGTAATCGATACAGGAGTCCATGCGCATGCTGATTTGACGGATCCTACAAATAGAATTGTAGCTTTTCAA
GATTTTATAAACGGAGAAAGCGAGCCTTATGATGATAATGGACATGGGACACATTGTGCTGGTGATGCGGCTGGAAATGG
CAGTCTCTCCAATGGGAAGTATATTGGACCGGCACCAGAGGCCTCTATTATCGGAATAAAGGTTTTGGATGAACAAGGGA
GTGGGAGACTATCCACTATCATTGAAGGTATAGAATGGTGTGTCGATCATAAAGAGGAGCATAATATACGGATTATTTCG
CTTTCTCTTGGAGCGCCAGCCTATGAATCTTTTAGGGATGATCCATTATCACTAGCTGCTCAATCTGCGTGGCATCAAGG
GATTGTAGTGTGTGCTGCAGCTGGAAACGATGGGCCTGCCGCATCAACGATCAGTACTCCAGCCATTGATCCATTTATTA
TTACGGTTGGATCGACGAATGATCAAAATACGTTAGAGCGTACGGATGATGCAATAGCAGAGTATTCTAGTAGAGGACCG
ACGATTGATACGCTGGTAAAACCGGATGTTTATGCCCCAGGTACAAATATTATTTCCTTGTCAGCCCCGGGATCAGCATT
GGAATCCCAAATCCCGGAGATGATCATTGATGAAAACTATATCCAATTGTCGGGCACATCAATGGCGACACCAATTTGTG
CAGGAATTATCGCCCTTATGCTGGAAGCTAATCCGAATCTCAGTCCGAATGATATAAAGAGCATCTTAAAAGCGACATCC
CATCCAACACTTGATGATATATGGGGATATATTGAAGCAGAAAGTGCCGTAGATATGGCAAAGAATTATACACGTCAAGG
TTCTGCTGAAGCAATACAAGAAAGTTAA

Upstream 100 bases:

>100_bases
TTAAATTTTCAAAAAATAGTCATCTTGTCTGATTGTTTCTACTACTTGTCCTTGATAATCTATTAGTGAACAAAATAAAA
CTAATCGAGGAGGAGTAGAA

Downstream 100 bases:

>100_bases
AGAAGGAAATGCGACCTAAATGCTAAGTACAGATGAAGGAAAATATAAATGCAAGCAATCCGAACTAATTCGAATCCTAG
ATTAGGTATTTGAATGTAGT

Product: intracellular alkaline serine proteinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSNRKKVWFEDAQRKLDPGLVEQLRNSRKVDQNETINNEIPLIVYMKKGCERGKKDDLLKACNIDSHNKLHRELRSINAM
KGYLTPDRIQQIKDHEAVDRIFYDRIVTAFLDIASEQTGAFKVQEQLGLTGKDVTIAVIDTGVHAHADLTDPTNRIVAFQ
DFINGESEPYDDNGHGTHCAGDAAGNGSLSNGKYIGPAPEASIIGIKVLDEQGSGRLSTIIEGIEWCVDHKEEHNIRIIS
LSLGAPAYESFRDDPLSLAAQSAWHQGIVVCAAAGNDGPAASTISTPAIDPFIITVGSTNDQNTLERTDDAIAEYSSRGP
TIDTLVKPDVYAPGTNIISLSAPGSALESQIPEMIIDENYIQLSGTSMATPICAGIIALMLEANPNLSPNDIKSILKATS
HPTLDDIWGYIEAESAVDMAKNYTRQGSAEAIQES

Sequences:

>Translated_435_residues
MSNRKKVWFEDAQRKLDPGLVEQLRNSRKVDQNETINNEIPLIVYMKKGCERGKKDDLLKACNIDSHNKLHRELRSINAM
KGYLTPDRIQQIKDHEAVDRIFYDRIVTAFLDIASEQTGAFKVQEQLGLTGKDVTIAVIDTGVHAHADLTDPTNRIVAFQ
DFINGESEPYDDNGHGTHCAGDAAGNGSLSNGKYIGPAPEASIIGIKVLDEQGSGRLSTIIEGIEWCVDHKEEHNIRIIS
LSLGAPAYESFRDDPLSLAAQSAWHQGIVVCAAAGNDGPAASTISTPAIDPFIITVGSTNDQNTLERTDDAIAEYSSRGP
TIDTLVKPDVYAPGTNIISLSAPGSALESQIPEMIIDENYIQLSGTSMATPICAGIIALMLEANPNLSPNDIKSILKATS
HPTLDDIWGYIEAESAVDMAKNYTRQGSAEAIQES
>Mature_434_residues
SNRKKVWFEDAQRKLDPGLVEQLRNSRKVDQNETINNEIPLIVYMKKGCERGKKDDLLKACNIDSHNKLHRELRSINAMK
GYLTPDRIQQIKDHEAVDRIFYDRIVTAFLDIASEQTGAFKVQEQLGLTGKDVTIAVIDTGVHAHADLTDPTNRIVAFQD
FINGESEPYDDNGHGTHCAGDAAGNGSLSNGKYIGPAPEASIIGIKVLDEQGSGRLSTIIEGIEWCVDHKEEHNIRIISL
SLGAPAYESFRDDPLSLAAQSAWHQGIVVCAAAGNDGPAASTISTPAIDPFIITVGSTNDQNTLERTDDAIAEYSSRGPT
IDTLVKPDVYAPGTNIISLSAPGSALESQIPEMIIDENYIQLSGTSMATPICAGIIALMLEANPNLSPNDIKSILKATSH
PTLDDIWGYIEAESAVDMAKNYTRQGSAEAIQES

Specific function: Displays serine protease activity. Seems to have a broad substrate specificity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasm (Probable). Note=Is leaked to the culture medium during the late stationary phase owing to cell lysis [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=320, Percent_Identity=26.5625, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI186972143, Length=222, Percent_Identity=28.8288288288288, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17533391, Length=266, Percent_Identity=25.5639097744361, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319893, Length=258, Percent_Identity=34.1085271317829, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320775, Length=330, Percent_Identity=31.5151515151515, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324576, Length=296, Percent_Identity=32.0945945945946, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45550681, Length=257, Percent_Identity=29.5719844357977, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24653270, Length=245, Percent_Identity=28.9795918367347, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI17137360, Length=245, Percent_Identity=28.9795918367347, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI281360987, Length=338, Percent_Identity=27.2189349112426, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24642494, Length=338, Percent_Identity=27.2189349112426, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24642490, Length=338, Percent_Identity=27.2189349112426, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45555723, Length=338, Percent_Identity=27.2189349112426, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24642492, Length=338, Percent_Identity=27.2189349112426, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24642488, Length=338, Percent_Identity=27.2189349112426, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24642484, Length=338, Percent_Identity=27.2189349112426, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24642486, Length=338, Percent_Identity=27.2189349112426, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8 [H]

EC number: NA

Molecular weight: Translated: 47196; Mature: 47065

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRKKVWFEDAQRKLDPGLVEQLRNSRKVDQNETINNEIPLIVYMKKGCERGKKDDLLK
CCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHH
ACNIDSHNKLHRELRSINAMKGYLTPDRIQQIKDHEAVDRIFYDRIVTAFLDIASEQTGA
HCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FKVQEQLGLTGKDVTIAVIDTGVHAHADLTDPTNRIVAFQDFINGESEPYDDNGHGTHCA
EEEHHHHCCCCCCEEEEEEECCCCCCCCCCCCCHHEEEEHHHHCCCCCCCCCCCCCCCCC
GDAAGNGSLSNGKYIGPAPEASIIGIKVLDEQGSGRLSTIIEGIEWCVDHKEEHNIRIIS
CCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
LSLGAPAYESFRDDPLSLAAQSAWHQGIVVCAAAGNDGPAASTISTPAIDPFIITVGSTN
EECCCCCHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCC
DQNTLERTDDAIAEYSSRGPTIDTLVKPDVYAPGTNIISLSAPGSALESQIPEMIIDENY
CCHHHHHHHHHHHHHHCCCCCEEHCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHCCCCE
IQLSGTSMATPICAGIIALMLEANPNLSPNDIKSILKATSHPTLDDIWGYIEAESAVDMA
EEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KNYTRQGSAEAIQES
HHHCCCCCHHHHHCC
>Mature Secondary Structure 
SNRKKVWFEDAQRKLDPGLVEQLRNSRKVDQNETINNEIPLIVYMKKGCERGKKDDLLK
CCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHH
ACNIDSHNKLHRELRSINAMKGYLTPDRIQQIKDHEAVDRIFYDRIVTAFLDIASEQTGA
HCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FKVQEQLGLTGKDVTIAVIDTGVHAHADLTDPTNRIVAFQDFINGESEPYDDNGHGTHCA
EEEHHHHCCCCCCEEEEEEECCCCCCCCCCCCCHHEEEEHHHHCCCCCCCCCCCCCCCCC
GDAAGNGSLSNGKYIGPAPEASIIGIKVLDEQGSGRLSTIIEGIEWCVDHKEEHNIRIIS
CCCCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
LSLGAPAYESFRDDPLSLAAQSAWHQGIVVCAAAGNDGPAASTISTPAIDPFIITVGSTN
EECCCCCHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCC
DQNTLERTDDAIAEYSSRGPTIDTLVKPDVYAPGTNIISLSAPGSALESQIPEMIIDENY
CCHHHHHHHHHHHHHHCCCCCEEHCCCCCCCCCCCCEEEECCCCHHHHHHCCHHHCCCCE
IQLSGTSMATPICAGIIALMLEANPNLSPNDIKSILKATSHPTLDDIWGYIEAESAVDMA
EEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KNYTRQGSAEAIQES
HHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]