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The map label for this gene is glcD [H]

Identifier: 23100286

GI number: 23100286

Start: 2910960

End: 2912360

Strand: Direct

Name: glcD [H]

Synonym: OB2831

Alternate gene names: 23100286

Gene position: 2910960-2912360 (Clockwise)

Preceding gene: 23100285

Following gene: 23100287

Centisome position: 80.18

GC content: 38.19

Gene sequence:

>1401_bases
ATGTTAGATAAATCAATTATTGAGCGTTTTAAAAACATTGTCGGAGAAAAAAATGTAGAAACTTCTAGTATGTCTCTTCA
TTCTTATAGCTATGATGCTACTCCAGGATTTCGAAAAACACCAGATATCGTTATCGCACCTCATAACACAGAAGAAATTG
CTGAAATCGTAAAAATTTGTAATGACCACAAGATTCCACTGACTCCTCGAGGTTCAGGTACGAATCTAAGTGCTGGAACC
ACGCCGCTCAAAGGTGGGGTTGTCTTACTTTTTAAACTTCTGAATAACATTCTAGAAATTGATGATCAAAACCTGACTGT
TACTGTACAACCAGGGGTAGTTACGAAATCATTAATTGAAAAAGTAGAAGCAAAAGGCTTACTCTATCCACCTGACCCGA
GTTCTATGAATATCTCTACGATCGGTGGAAATATTAATGAAAATTCTGGTGGGTTGCGTGGACTTAAATACGGTGTAACG
AGAGATTACGTTCTTGGTTTAACGATTGTTTTGCCAAATGGCGACATTATTAAAACAGGCGGAAAGCTAGCCAAAGATGT
AGCTGGATATGATTTAACTCGATTATTTGTCGGCTCAGAAGGTACGCTTGGAATTATTACCGAAGCTATTCTGAAATTAG
TACCGATGCCAGAATATAAAAAAACAATGCTTGCTACCTATGAAGACATTGACGACGCAGCAAAAACGGTATCCGCAATC
ATCGAAAATCGAATCATCCCAGCAACCTTAGAATTTTTAGATCAACCGACCGTGGAAGTCGTGGAAGAATATAATCAAAT
TGGTTTACCAACAGATGCAAAAGCTATTTTATTAATCGAGCAAGATGGTCCAGAATCAGTCGTAAATAAAGATATAGATA
AAATCGCAGAAATATGTAAGGAATTACATGCAGTAACTGTAAGTGTTGCTAAAAACGAACAGGAAGCAACAGCGCTCACG
ACTGCACGTAGGTCAGCACTGTCTACGATTGCTCGCTTGCGACCAACCACCTTTTTAGAAGATGCCACCGTACCAAGATC
ACAAGTTGCTCCAATGGTAAAAGAGATTAATAGAATCGCTGAAAAATATAATGTAAAAATATGTACATTTGGTCACGCCG
GTGATGGGAATCTTCACCCTACCGTTCCAACTGATGTCCGAGATAAAGAAGAATATCATCGTGTTGAACAAGCTTTTGCT
GAGATATTTGAAAAAGCAGTAGAACTCGGTGGAACAATAACTGGCGAACATGGTGTCGGTGCAATGAAAGCACCTTTTTT
AAAACTTAAATTAGGAGAAGAAGGAGTTCGTGCTTTAAAGAACATCAAACAAAGCTTTGACCCTAACAATATTATGAACC
CTGGAAAAATGTTTTTTGAAGACGATGAGAAAGGAGAGTAA

Upstream 100 bases:

>100_bases
ATCATCTTTTTCGTATAAAAACACATAGAATTTTCTATGTGTTTTTATTTATACTATATAAATTGTATAAATAATTAAAG
CCCGCAGGAGGTTACTAATT

Downstream 100 bases:

>100_bases
AACGTGGATACTTTAGTAAAAGAAAGGATTCAAGAAGACTTTCAGAATCGGATGGACAGAGATGAATTGGAAAATTGTAT
GCGCTGTGGATTTTGTCTGC

Product: glycolate oxidase subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MLDKSIIERFKNIVGEKNVETSSMSLHSYSYDATPGFRKTPDIVIAPHNTEEIAEIVKICNDHKIPLTPRGSGTNLSAGT
TPLKGGVVLLFKLLNNILEIDDQNLTVTVQPGVVTKSLIEKVEAKGLLYPPDPSSMNISTIGGNINENSGGLRGLKYGVT
RDYVLGLTIVLPNGDIIKTGGKLAKDVAGYDLTRLFVGSEGTLGIITEAILKLVPMPEYKKTMLATYEDIDDAAKTVSAI
IENRIIPATLEFLDQPTVEVVEEYNQIGLPTDAKAILLIEQDGPESVVNKDIDKIAEICKELHAVTVSVAKNEQEATALT
TARRSALSTIARLRPTTFLEDATVPRSQVAPMVKEINRIAEKYNVKICTFGHAGDGNLHPTVPTDVRDKEEYHRVEQAFA
EIFEKAVELGGTITGEHGVGAMKAPFLKLKLGEEGVRALKNIKQSFDPNNIMNPGKMFFEDDEKGE

Sequences:

>Translated_466_residues
MLDKSIIERFKNIVGEKNVETSSMSLHSYSYDATPGFRKTPDIVIAPHNTEEIAEIVKICNDHKIPLTPRGSGTNLSAGT
TPLKGGVVLLFKLLNNILEIDDQNLTVTVQPGVVTKSLIEKVEAKGLLYPPDPSSMNISTIGGNINENSGGLRGLKYGVT
RDYVLGLTIVLPNGDIIKTGGKLAKDVAGYDLTRLFVGSEGTLGIITEAILKLVPMPEYKKTMLATYEDIDDAAKTVSAI
IENRIIPATLEFLDQPTVEVVEEYNQIGLPTDAKAILLIEQDGPESVVNKDIDKIAEICKELHAVTVSVAKNEQEATALT
TARRSALSTIARLRPTTFLEDATVPRSQVAPMVKEINRIAEKYNVKICTFGHAGDGNLHPTVPTDVRDKEEYHRVEQAFA
EIFEKAVELGGTITGEHGVGAMKAPFLKLKLGEEGVRALKNIKQSFDPNNIMNPGKMFFEDDEKGE
>Mature_466_residues
MLDKSIIERFKNIVGEKNVETSSMSLHSYSYDATPGFRKTPDIVIAPHNTEEIAEIVKICNDHKIPLTPRGSGTNLSAGT
TPLKGGVVLLFKLLNNILEIDDQNLTVTVQPGVVTKSLIEKVEAKGLLYPPDPSSMNISTIGGNINENSGGLRGLKYGVT
RDYVLGLTIVLPNGDIIKTGGKLAKDVAGYDLTRLFVGSEGTLGIITEAILKLVPMPEYKKTMLATYEDIDDAAKTVSAI
IENRIIPATLEFLDQPTVEVVEEYNQIGLPTDAKAILLIEQDGPESVVNKDIDKIAEICKELHAVTVSVAKNEQEATALT
TARRSALSTIARLRPTTFLEDATVPRSQVAPMVKEINRIAEKYNVKICTFGHAGDGNLHPTVPTDVRDKEEYHRVEQAFA
EIFEKAVELGGTITGEHGVGAMKAPFLKLKLGEEGVRALKNIKQSFDPNNIMNPGKMFFEDDEKGE

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=466, Percent_Identity=31.9742489270386, Blast_Score=258, Evalue=8e-69,
Organism=Homo sapiens, GI37595754, Length=489, Percent_Identity=30.4703476482618, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI119964728, Length=463, Percent_Identity=26.3498920086393, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI4501993, Length=469, Percent_Identity=23.8805970149254, Blast_Score=135, Evalue=6e-32,
Organism=Escherichia coli, GI1789351, Length=428, Percent_Identity=35.5140186915888, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI1787977, Length=503, Percent_Identity=25.6461232604374, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI48994907, Length=486, Percent_Identity=25.3086419753086, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI48994914, Length=199, Percent_Identity=30.6532663316583, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71992373, Length=460, Percent_Identity=32.1739130434783, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI71992381, Length=460, Percent_Identity=31.7391304347826, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI71992392, Length=370, Percent_Identity=32.7027027027027, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17534361, Length=467, Percent_Identity=28.051391862955, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17556096, Length=466, Percent_Identity=24.6781115879828, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320027, Length=474, Percent_Identity=31.8565400843882, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6320764, Length=479, Percent_Identity=28.39248434238, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6320023, Length=473, Percent_Identity=29.1754756871036, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI18921117, Length=464, Percent_Identity=26.0775862068966, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24639277, Length=464, Percent_Identity=26.0775862068966, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24639275, Length=464, Percent_Identity=26.0775862068966, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24653753, Length=474, Percent_Identity=22.5738396624473, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 50778; Mature: 50778

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKSIIERFKNIVGEKNVETSSMSLHSYSYDATPGFRKTPDIVIAPHNTEEIAEIVKIC
CCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
NDHKIPLTPRGSGTNLSAGTTPLKGGVVLLFKLLNNILEIDDQNLTVTVQPGVVTKSLIE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
KVEAKGLLYPPDPSSMNISTIGGNINENSGGLRGLKYGVTRDYVLGLTIVLPNGDIIKTG
HHHHCCCEECCCCCCCEEEEECCCCCCCCCCCCCEECCCCCCEEEEEEEEECCCCEEECC
GKLAKDVAGYDLTRLFVGSEGTLGIITEAILKLVPMPEYKKTMLATYEDIDDAAKTVSAI
CHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IENRIIPATLEFLDQPTVEVVEEYNQIGLPTDAKAILLIEQDGPESVVNKDIDKIAEICK
HHCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
ELHAVTVSVAKNEQEATALTTARRSALSTIARLRPTTFLEDATVPRSQVAPMVKEINRIA
HHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHH
EKYNVKICTFGHAGDGNLHPTVPTDVRDKEEYHRVEQAFAEIFEKAVELGGTITGEHGVG
HHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
AMKAPFLKLKLGEEGVRALKNIKQSFDPNNIMNPGKMFFEDDEKGE
CCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCC
>Mature Secondary Structure
MLDKSIIERFKNIVGEKNVETSSMSLHSYSYDATPGFRKTPDIVIAPHNTEEIAEIVKIC
CCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
NDHKIPLTPRGSGTNLSAGTTPLKGGVVLLFKLLNNILEIDDQNLTVTVQPGVVTKSLIE
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
KVEAKGLLYPPDPSSMNISTIGGNINENSGGLRGLKYGVTRDYVLGLTIVLPNGDIIKTG
HHHHCCCEECCCCCCCEEEEECCCCCCCCCCCCCEECCCCCCEEEEEEEEECCCCEEECC
GKLAKDVAGYDLTRLFVGSEGTLGIITEAILKLVPMPEYKKTMLATYEDIDDAAKTVSAI
CHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IENRIIPATLEFLDQPTVEVVEEYNQIGLPTDAKAILLIEQDGPESVVNKDIDKIAEICK
HHCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
ELHAVTVSVAKNEQEATALTTARRSALSTIARLRPTTFLEDATVPRSQVAPMVKEINRIA
HHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHH
EKYNVKICTFGHAGDGNLHPTVPTDVRDKEEYHRVEQAFAEIFEKAVELGGTITGEHGVG
HHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCC
AMKAPFLKLKLGEEGVRALKNIKQSFDPNNIMNPGKMFFEDDEKGE
CCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]