Click here to switch to the map view.

The map label for this gene is glcF [H]

Identifier: 23100287

GI number: 23100287

Start: 2912364

End: 2913695

Strand: Direct

Name: glcF [H]

Synonym: OB2832

Alternate gene names: 23100287

Gene position: 2912364-2913695 (Clockwise)

Preceding gene: 23100286

Following gene: 23100290

Centisome position: 80.22

GC content: 41.89

Gene sequence:

>1332_bases
GTGGATACTTTAGTAAAAGAAAGGATTCAAGAAGACTTTCAGAATCGGATGGACAGAGATGAATTGGAAAATTGTATGCG
CTGTGGATTTTGTCTGCCTGTTTGTCCGACCTATATTCAATCAGGTTATGACGAAACGCAATCTCCCAGGGGACGGATTG
CATTAATGAAAGGCATTGTTGATGGCGAAATCGAACCAGATGAAGATATTGAACAAACATTAAATGAATGCCTTGGATGT
AGAGCCTGTGAACCTGCCTGTCCTGCTGGAGTGAACTATGGACAGCTGCTAGAGGAAGCAAGAGATATTTTCAATCAGAA
TAAGCAACATTCCCTTCCAGAAAGAGTGATTAGAAAGACTGTCTTTGAGGGATTATTTCCACATCCAAATAGAATGGAAA
ACATGACCTCTCTTCTCACCTTCTATCAGCGCTCTGGACTGCAAAAGGTTGCTAGAAATATTGGTTTCATGAAGCTATTT
CCAGATACATTGGCGACTATGGAAAAAGTATTACCGACCACACCAACACGGAAAGAAATGCGTTCTCAATCTTCCTACTA
TGTACATAAAAGCCAACCAAAAGCGACCGTTGCATTTTTCAAAGGCTGTTTGATGGATACCATGTTTCGTGAAGTGAATA
AAGCAACCATTGAACTCTTGCAAAAAGTCGGCTGTGACATCGCCGTCCCTGACACTCAAAATTGTTGCGGTGCCTTGCAC
GGTCACTCTGGAGAAAAAGAAAAAGCGAAAGAACTAGCCAAAAAAAACATTGCTGCCTTTGAACAAATCGGTGCAGATTA
TATCATTACAAACGCTGGTGGCTGTGGTGCATTTCTCCATGATTATGGTCATCTGCTGCACGATGATCCAGAGTGGTCAG
AGAGAGCATCACACTTTTCCTCAAAAATTAAAGATATTACGGCAATTCTTATCGACCTAGATTTCCATATGATGCCACTA
CAAATGCCGGAACAGGTTGTTACTTACCAAGATTCGTGCCATTTACGTAATGTTCAAAAGACATTTGAAGAGCCTCGACT
ATTACTGGAGTCGATCGATGGCTTCCATTACGAAGAGATGAACGATGCAGCAAGATGTTGCGGCTCAGCGGGAATATATA
ACATCGTCCATTCCGAACTATCCATGAAACATTTGGATTACAAAATGAAGCAAGTGAAAGACACTGCTGCAACAACCATT
GTAACCACCAACCCTGGTTGCCTCCTGCAAATGAAATTAGGCATCGAACGAGAAGGCTTGTCTAATAAAGTGCGTGCTGT
ACACATTGTGGAACTGTTAATCGAGGCCGTCGATCGTAGAACAAGTATGTAA

Upstream 100 bases:

>100_bases
AGTTCGTGCTTTAAAGAACATCAAACAAAGCTTTGACCCTAACAATATTATGAACCCTGGAAAAATGTTTTTTGAAGACG
ATGAGAAAGGAGAGTAAAAC

Downstream 100 bases:

>100_bases
ATGACTATGTAGACTAAAACCCAAACTACTTGATTATATCTACCAGTTTGGGTTTTAGAGCCAGTCTATTGACCAATTTT
CCGGATTTCTCTTGCTTTTT

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MDTLVKERIQEDFQNRMDRDELENCMRCGFCLPVCPTYIQSGYDETQSPRGRIALMKGIVDGEIEPDEDIEQTLNECLGC
RACEPACPAGVNYGQLLEEARDIFNQNKQHSLPERVIRKTVFEGLFPHPNRMENMTSLLTFYQRSGLQKVARNIGFMKLF
PDTLATMEKVLPTTPTRKEMRSQSSYYVHKSQPKATVAFFKGCLMDTMFREVNKATIELLQKVGCDIAVPDTQNCCGALH
GHSGEKEKAKELAKKNIAAFEQIGADYIITNAGGCGAFLHDYGHLLHDDPEWSERASHFSSKIKDITAILIDLDFHMMPL
QMPEQVVTYQDSCHLRNVQKTFEEPRLLLESIDGFHYEEMNDAARCCGSAGIYNIVHSELSMKHLDYKMKQVKDTAATTI
VTTNPGCLLQMKLGIEREGLSNKVRAVHIVELLIEAVDRRTSM

Sequences:

>Translated_443_residues
MDTLVKERIQEDFQNRMDRDELENCMRCGFCLPVCPTYIQSGYDETQSPRGRIALMKGIVDGEIEPDEDIEQTLNECLGC
RACEPACPAGVNYGQLLEEARDIFNQNKQHSLPERVIRKTVFEGLFPHPNRMENMTSLLTFYQRSGLQKVARNIGFMKLF
PDTLATMEKVLPTTPTRKEMRSQSSYYVHKSQPKATVAFFKGCLMDTMFREVNKATIELLQKVGCDIAVPDTQNCCGALH
GHSGEKEKAKELAKKNIAAFEQIGADYIITNAGGCGAFLHDYGHLLHDDPEWSERASHFSSKIKDITAILIDLDFHMMPL
QMPEQVVTYQDSCHLRNVQKTFEEPRLLLESIDGFHYEEMNDAARCCGSAGIYNIVHSELSMKHLDYKMKQVKDTAATTI
VTTNPGCLLQMKLGIEREGLSNKVRAVHIVELLIEAVDRRTSM
>Mature_443_residues
MDTLVKERIQEDFQNRMDRDELENCMRCGFCLPVCPTYIQSGYDETQSPRGRIALMKGIVDGEIEPDEDIEQTLNECLGC
RACEPACPAGVNYGQLLEEARDIFNQNKQHSLPERVIRKTVFEGLFPHPNRMENMTSLLTFYQRSGLQKVARNIGFMKLF
PDTLATMEKVLPTTPTRKEMRSQSSYYVHKSQPKATVAFFKGCLMDTMFREVNKATIELLQKVGCDIAVPDTQNCCGALH
GHSGEKEKAKELAKKNIAAFEQIGADYIITNAGGCGAFLHDYGHLLHDDPEWSERASHFSSKIKDITAILIDLDFHMMPL
QMPEQVVTYQDSCHLRNVQKTFEEPRLLLESIDGFHYEEMNDAARCCGSAGIYNIVHSELSMKHLDYKMKQVKDTAATTI
VTTNPGCLLQMKLGIEREGLSNKVRAVHIVELLIEAVDRRTSM

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=442, Percent_Identity=29.185520361991, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1786497, Length=239, Percent_Identity=28.8702928870293, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788576, Length=434, Percent_Identity=24.4239631336406, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 50160; Mature: 50160

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLVKERIQEDFQNRMDRDELENCMRCGFCLPVCPTYIQSGYDETQSPRGRIALMKGIV
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
DGEIEPDEDIEQTLNECLGCRACEPACPAGVNYGQLLEEARDIFNQNKQHSLPERVIRKT
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VFEGLFPHPNRMENMTSLLTFYQRSGLQKVARNIGFMKLFPDTLATMEKVLPTTPTRKEM
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHH
RSQSSYYVHKSQPKATVAFFKGCLMDTMFREVNKATIELLQKVGCDIAVPDTQNCCGALH
HCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHC
GHSGEKEKAKELAKKNIAAFEQIGADYIITNAGGCGAFLHDYGHLLHDDPEWSERASHFS
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
SKIKDITAILIDLDFHMMPLQMPEQVVTYQDSCHLRNVQKTFEEPRLLLESIDGFHYEEM
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
NDAARCCGSAGIYNIVHSELSMKHLDYKMKQVKDTAATTIVTTNPGCLLQMKLGIEREGL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCC
SNKVRAVHIVELLIEAVDRRTSM
CHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDTLVKERIQEDFQNRMDRDELENCMRCGFCLPVCPTYIQSGYDETQSPRGRIALMKGIV
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
DGEIEPDEDIEQTLNECLGCRACEPACPAGVNYGQLLEEARDIFNQNKQHSLPERVIRKT
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VFEGLFPHPNRMENMTSLLTFYQRSGLQKVARNIGFMKLFPDTLATMEKVLPTTPTRKEM
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHH
RSQSSYYVHKSQPKATVAFFKGCLMDTMFREVNKATIELLQKVGCDIAVPDTQNCCGALH
HCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHC
GHSGEKEKAKELAKKNIAAFEQIGADYIITNAGGCGAFLHDYGHLLHDDPEWSERASHFS
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
SKIKDITAILIDLDFHMMPLQMPEQVVTYQDSCHLRNVQKTFEEPRLLLESIDGFHYEEM
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
NDAARCCGSAGIYNIVHSELSMKHLDYKMKQVKDTAATTIVTTNPGCLLQMKLGIEREGL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCCCCC
SNKVRAVHIVELLIEAVDRRTSM
CHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]