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The map label for this gene is pgk

Identifier: 23099892

GI number: 23099892

Start: 2492179

End: 2493360

Strand: Reverse

Name: pgk

Synonym: OB2437

Alternate gene names: 23099892

Gene position: 2493360-2492179 (Counterclockwise)

Preceding gene: 23099893

Following gene: 23099891

Centisome position: 68.68

GC content: 38.49

Gene sequence:

>1182_bases
TTGAACAAAAAAACAATTAACGATCTACAAGTTGAAGGAAAGCGAGTATTTACACGCGTAGACTTTAATGTTCCTTTAAA
AGATGGTGAGATTACAGATGATACACGTATTCGCGCCGCACTACCGACAATTGAACAATTAACAGCACAAGGTGCAAAAG
TGATTTTAGCTAGTCATCTAGGACGACCTAAAGGAACAGTTGTAGAAGAACTACGTTTAGACCCTGTTGCTGATAGACTT
AGTGATCTATTAGGTAAAGAAGTATTGAAAACAGATCAAGTTTATGGTGAAGAAGTTGATCAGGCTATCAGTGAGCTAGA
AAACGGTGGAGTTCTTTTAATAGAGAACGTTCGCTTTGAAGCTGGAGAAGAAAAAAATGATCCTGAATTAGTGGAAGCAT
TCGCTAACATGGCTGACCTTTATGTCAATGATGCTTTTGGTGCTGCTCATCGTGCACATGCATCTACAACTGGCGTAGCA
GAGAAATTACCAGCAGCTGCAGGTAAATTAATGGAGAAAGAAATTGAAGCACTAGGCAACGCTCTAGAAAATCCGGAACG
ACCGTTTACAGCAATTGTTGGTGGAGCGAAAGTAAAAGATAAAATTAGTGTGATCGATAATCTGTTAGATAAAGTAGATA
ATTTGATTATTGGTGGCGGTCTAGCTTACACGTTCATCAAAGCACAAGGTCATGAGATTGGAAAATCACTTCTAGAAGAA
GATAAAATTGATTTGGCTAAAGAATTTATGAACAAAGCGAAAGAAAAAGGTGTTAACTTTGTATTGCCAGTCGACGCTAT
TGTGGCCGATGATTTCTCAGAAGAGGCTAATACCAAAATTGTAGATATAGATAAAATTCCTTCTGACTGGGAAGCGTTAG
ATATAGGCACGAAGACAAGAGAAAAATATAGTAGTATCGTGAAAGATTCAAAACTTGTTATATGGAATGGACCAATGGGA
GTATTTGAATTAAATGCATTCGCAGGTGGTACAAAAGCTGTAGCAGAAGCATTAGCATCTACAGAAGGCTACTCTATAAT
AGGTGGTGGCGATTCTGCGGCAGCTGTTGAAAAGTTCGGCCTTGCAGAAGAAATGGATCATGTATCTACAGGTGGAGGAG
CATCATTAGAATTCATGGAAGGAAAAGTTCTTCCTGGACTAGCTGCTCTAAATGATAAATAA

Upstream 100 bases:

>100_bases
GTTTTAATCAGTATTGTTTTATATTGACTGCTACGTGGTATAATATTGCTTGGTTATGAATGATTATGTATTACATAATC
CAGTATTAGGAGGGATTATC

Downstream 100 bases:

>100_bases
TAATAAATAACTAACAGAGGTGATTCGATGCGTAAAAAAGTGATAGCTGGTAACTGGAAAATGAATAAAGTACTATCTGA
AGCAGGAGAATTTATGAATT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNKKTINDLQVEGKRVFTRVDFNVPLKDGEITDDTRIRAALPTIEQLTAQGAKVILASHLGRPKGTVVEELRLDPVADRL
SDLLGKEVLKTDQVYGEEVDQAISELENGGVLLIENVRFEAGEEKNDPELVEAFANMADLYVNDAFGAAHRAHASTTGVA
EKLPAAAGKLMEKEIEALGNALENPERPFTAIVGGAKVKDKISVIDNLLDKVDNLIIGGGLAYTFIKAQGHEIGKSLLEE
DKIDLAKEFMNKAKEKGVNFVLPVDAIVADDFSEEANTKIVDIDKIPSDWEALDIGTKTREKYSSIVKDSKLVIWNGPMG
VFELNAFAGGTKAVAEALASTEGYSIIGGGDSAAAVEKFGLAEEMDHVSTGGGASLEFMEGKVLPGLAALNDK

Sequences:

>Translated_393_residues
MNKKTINDLQVEGKRVFTRVDFNVPLKDGEITDDTRIRAALPTIEQLTAQGAKVILASHLGRPKGTVVEELRLDPVADRL
SDLLGKEVLKTDQVYGEEVDQAISELENGGVLLIENVRFEAGEEKNDPELVEAFANMADLYVNDAFGAAHRAHASTTGVA
EKLPAAAGKLMEKEIEALGNALENPERPFTAIVGGAKVKDKISVIDNLLDKVDNLIIGGGLAYTFIKAQGHEIGKSLLEE
DKIDLAKEFMNKAKEKGVNFVLPVDAIVADDFSEEANTKIVDIDKIPSDWEALDIGTKTREKYSSIVKDSKLVIWNGPMG
VFELNAFAGGTKAVAEALASTEGYSIIGGGDSAAAVEKFGLAEEMDHVSTGGGASLEFMEGKVLPGLAALNDK
>Mature_393_residues
MNKKTINDLQVEGKRVFTRVDFNVPLKDGEITDDTRIRAALPTIEQLTAQGAKVILASHLGRPKGTVVEELRLDPVADRL
SDLLGKEVLKTDQVYGEEVDQAISELENGGVLLIENVRFEAGEEKNDPELVEAFANMADLYVNDAFGAAHRAHASTTGVA
EKLPAAAGKLMEKEIEALGNALENPERPFTAIVGGAKVKDKISVIDNLLDKVDNLIIGGGLAYTFIKAQGHEIGKSLLEE
DKIDLAKEFMNKAKEKGVNFVLPVDAIVADDFSEEANTKIVDIDKIPSDWEALDIGTKTREKYSSIVKDSKLVIWNGPMG
VFELNAFAGGTKAVAEALASTEGYSIIGGGDSAAAVEKFGLAEEMDHVSTGGGASLEFMEGKVLPGLAALNDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=48.3091787439614, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI31543397, Length=413, Percent_Identity=46.4891041162228, Blast_Score=352, Evalue=3e-97,
Organism=Escherichia coli, GI1789294, Length=395, Percent_Identity=47.3417721518987, Blast_Score=348, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=47.5728155339806, Blast_Score=358, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6319857, Length=413, Percent_Identity=49.1525423728814, Blast_Score=362, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=44.578313253012, Blast_Score=330, Evalue=9e-91,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=45.985401459854, Blast_Score=328, Evalue=5e-90,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_OCEIH (Q8ENP3)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693358.1
- ProteinModelPortal:   Q8ENP3
- SMR:   Q8ENP3
- GeneID:   1017220
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2437
- NMPDR:   fig|221109.1.peg.2434
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   OIHE221109:OB2437-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42119; Mature: 42119

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 118-118 BINDING 151-151 BINDING 201-201 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKTINDLQVEGKRVFTRVDFNVPLKDGEITDDTRIRAALPTIEQLTAQGAKVILASHL
CCCCCCCHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHHCCHHHHHHHHC
GRPKGTVVEELRLDPVADRLSDLLGKEVLKTDQVYGEEVDQAISELENGGVLLIENVRFE
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEC
AGEEKNDPELVEAFANMADLYVNDAFGAAHRAHASTTGVAEKLPAAAGKLMEKEIEALGN
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALENPERPFTAIVGGAKVKDKISVIDNLLDKVDNLIIGGGLAYTFIKAQGHEIGKSLLEE
HHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCEEECCCCEEEEEECCCHHHHHHHHHH
DKIDLAKEFMNKAKEKGVNFVLPVDAIVADDFSEEANTKIVDIDKIPSDWEALDIGTKTR
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCHHHCCEEEEECCCCCCCCEEECCCHHH
EKYSSIVKDSKLVIWNGPMGVFELNAFAGGTKAVAEALASTEGYSIIGGGDSAAAVEKFG
HHHHHHHCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHC
LAEEMDHVSTGGGASLEFMEGKVLPGLAALNDK
CHHHHHHCCCCCCCEEEEECCCCCCCHHHCCCC
>Mature Secondary Structure
MNKKTINDLQVEGKRVFTRVDFNVPLKDGEITDDTRIRAALPTIEQLTAQGAKVILASHL
CCCCCCCHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHHHCCHHHHHHHHC
GRPKGTVVEELRLDPVADRLSDLLGKEVLKTDQVYGEEVDQAISELENGGVLLIENVRFE
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEC
AGEEKNDPELVEAFANMADLYVNDAFGAAHRAHASTTGVAEKLPAAAGKLMEKEIEALGN
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALENPERPFTAIVGGAKVKDKISVIDNLLDKVDNLIIGGGLAYTFIKAQGHEIGKSLLEE
HHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCEEECCCCEEEEEECCCHHHHHHHHHH
DKIDLAKEFMNKAKEKGVNFVLPVDAIVADDFSEEANTKIVDIDKIPSDWEALDIGTKTR
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHCCCCHHHCCEEEEECCCCCCCCEEECCCHHH
EKYSSIVKDSKLVIWNGPMGVFELNAFAGGTKAVAEALASTEGYSIIGGGDSAAAVEKFG
HHHHHHHCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHC
LAEEMDHVSTGGGASLEFMEGKVLPGLAALNDK
CHHHHHHCCCCCCCEEEEECCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376