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The map label for this gene is tpi

Identifier: 23099891

GI number: 23099891

Start: 2491393

End: 2492151

Strand: Reverse

Name: tpi

Synonym: OB2436

Alternate gene names: 23099891

Gene position: 2492151-2491393 (Counterclockwise)

Preceding gene: 23099892

Following gene: 23099890

Centisome position: 68.64

GC content: 40.05

Gene sequence:

>759_bases
ATGCGTAAAAAAGTGATAGCTGGTAACTGGAAAATGAATAAAGTACTATCTGAAGCAGGAGAATTTATGAATTCTGTAGT
ACCAAAAGCTCCGAAAGGAAATGACGTAGAAGCAATTGTGTGTGCTCCATTCCCTTTCCTGTCAAAATTAGTTGAGCAAG
CGAAGGGATCAGAAGTAAAAGTAGCAGCGCAAAACATGCATTTTGAAGATTCTGGTGCTTTTACTGGAGAGGTTAGCCCT
GTGATGTTGAAAGATCTAGGTGTAACACATGTTGTACTTGGACACTCAGAAAGAAGAGAGCTATTTGCTGAAACGAACGA
ATTAGTAAATAAGAAAACGAAAGCAGCATTTGCTCATGATTTAACTCCGATTGTTTGTGTAGGAGAAACACTGGATCAAC
GTGAAGCAAACGAAACGATGAACATTGTTGGCGAACAAGTAAAGCAAGCAGTTGCTGGTCTTACCAATGAACAAGTTGCT
GAAACTATTATTGCCTATGAACCAGTATGGGCAATCGGTACAGGAAAAACAGCTACAAGTGAACAAGCAAATGAAGTTTG
CACTGAAATTCGCAAAGTGGTAGCTGAAGTTACGTCAGCAGATGTGGCTGAAAAAGTAATTATTCAATATGGCGGTAGTG
TAAAACCTGCCAATGTGGATGAGTTATTAGCTCAATCTGATATTGACGGAGCATTAGTTGGTGGGGCAAGTCTCGATCCT
GAATCATTCCTACAACTAGTGGAGGCAGGTACAAAATGA

Upstream 100 bases:

>100_bases
AGGTGGAGGAGCATCATTAGAATTCATGGAAGGAAAAGTTCTTCCTGGACTAGCTGCTCTAAATGATAAATAATAATAAA
TAACTAACAGAGGTGATTCG

Downstream 100 bases:

>100_bases
GTCAGAATAAATTAGCTGCATTAATTATTCTTGATGGATTCGCTATTCGTGATGAAGTAAAAGGAAATGCAGTCAAACAA
GCAAACACCCCTAATTTTGA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRKKVIAGNWKMNKVLSEAGEFMNSVVPKAPKGNDVEAIVCAPFPFLSKLVEQAKGSEVKVAAQNMHFEDSGAFTGEVSP
VMLKDLGVTHVVLGHSERRELFAETNELVNKKTKAAFAHDLTPIVCVGETLDQREANETMNIVGEQVKQAVAGLTNEQVA
ETIIAYEPVWAIGTGKTATSEQANEVCTEIRKVVAEVTSADVAEKVIIQYGGSVKPANVDELLAQSDIDGALVGGASLDP
ESFLQLVEAGTK

Sequences:

>Translated_252_residues
MRKKVIAGNWKMNKVLSEAGEFMNSVVPKAPKGNDVEAIVCAPFPFLSKLVEQAKGSEVKVAAQNMHFEDSGAFTGEVSP
VMLKDLGVTHVVLGHSERRELFAETNELVNKKTKAAFAHDLTPIVCVGETLDQREANETMNIVGEQVKQAVAGLTNEQVA
ETIIAYEPVWAIGTGKTATSEQANEVCTEIRKVVAEVTSADVAEKVIIQYGGSVKPANVDELLAQSDIDGALVGGASLDP
ESFLQLVEAGTK
>Mature_252_residues
MRKKVIAGNWKMNKVLSEAGEFMNSVVPKAPKGNDVEAIVCAPFPFLSKLVEQAKGSEVKVAAQNMHFEDSGAFTGEVSP
VMLKDLGVTHVVLGHSERRELFAETNELVNKKTKAAFAHDLTPIVCVGETLDQREANETMNIVGEQVKQAVAGLTNEQVA
ETIIAYEPVWAIGTGKTATSEQANEVCTEIRKVVAEVTSADVAEKVIIQYGGSVKPANVDELLAQSDIDGALVGGASLDP
ESFLQLVEAGTK

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=46.1847389558233, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=46.1847389558233, Blast_Score=199, Evalue=2e-51,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=41.7670682730924, Blast_Score=194, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17536593, Length=252, Percent_Identity=43.6507936507937, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320255, Length=255, Percent_Identity=38.8235294117647, Blast_Score=169, Evalue=4e-43,
Organism=Drosophila melanogaster, GI28572008, Length=248, Percent_Identity=42.3387096774194, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI28572006, Length=248, Percent_Identity=42.3387096774194, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI28572004, Length=248, Percent_Identity=42.3387096774194, Blast_Score=187, Evalue=7e-48,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_OCEIH (Q8ENP4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693357.1
- ProteinModelPortal:   Q8ENP4
- SMR:   Q8ENP4
- GeneID:   1015415
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2436
- NMPDR:   fig|221109.1.peg.2433
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- BioCyc:   OIHE221109:OB2436-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26923; Mature: 26923

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKVIAGNWKMNKVLSEAGEFMNSVVPKAPKGNDVEAIVCAPFPFLSKLVEQAKGSEVK
CCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCEEE
VAAQNMHFEDSGAFTGEVSPVMLKDLGVTHVVLGHSERRELFAETNELVNKKTKAAFAHD
EECCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
LTPIVCVGETLDQREANETMNIVGEQVKQAVAGLTNEQVAETIIAYEPVWAIGTGKTATS
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCH
EQANEVCTEIRKVVAEVTSADVAEKVIIQYGGSVKPANVDELLAQSDIDGALVGGASLDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEECCCCCCH
ESFLQLVEAGTK
HHHHHHHHCCCC
>Mature Secondary Structure
MRKKVIAGNWKMNKVLSEAGEFMNSVVPKAPKGNDVEAIVCAPFPFLSKLVEQAKGSEVK
CCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCEEE
VAAQNMHFEDSGAFTGEVSPVMLKDLGVTHVVLGHSERRELFAETNELVNKKTKAAFAHD
EECCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
LTPIVCVGETLDQREANETMNIVGEQVKQAVAGLTNEQVAETIIAYEPVWAIGTGKTATS
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEECCCCCCCH
EQANEVCTEIRKVVAEVTSADVAEKVIIQYGGSVKPANVDELLAQSDIDGALVGGASLDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEECCCCCCH
ESFLQLVEAGTK
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376