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The map label for this gene is eno

Identifier: 23099889

GI number: 23099889

Start: 2488545

End: 2489834

Strand: Reverse

Name: eno

Synonym: OB2434

Alternate gene names: 23099889

Gene position: 2489834-2488545 (Counterclockwise)

Preceding gene: 23099890

Following gene: 23099888

Centisome position: 68.58

GC content: 38.45

Gene sequence:

>1290_bases
ATGCCATATATTACAGATGTTTATGCTAGAGAAGTCTTAGATTCAAGAGGTAACCCAACCATTGAAGTTGAAATTTTCAC
AGAGTCAGGAGCATTTGGTTCAGCAATCGTACCAAGTGGTGCATCAACTGGTGAGTATGAAGCAGTAGAACTTCGTGATG
GTGACAAAGATCGTTATTTAGGTAAAGGAGTTCAAAAAGCAGTTGAAAATGTAAATGATCTAATTGCGCCTGAACTTATT
GGTATTGATGTTACGCGTCAAAACATTATTGATGCTTTAATGATCGACCTAGATGGAACGGAAAACAAAGGTAAACTAGG
TGCGAATGCAATCCTTGGAGTATCCATGGCAGCAGCACATGCAGCAGCAAACTATTTAGAAGTACCTCTATACAACTATT
TAGGTGGGTTCAATGCAAAAACACTTCCAACACCAATGATGAACATATTAAATGGTGGAGAGCATGCGGATAACAACGTA
GATATTCAAGAATTTATGATTATGCCTGTTGGTGCTCCAACATTTAAAGAAGCATTGCGCACTGGTGCTGAAATTTTCCA
TGCGTTGAAAAAAGTACTAACTTCAAAAGGCTATAACACTGCAGTAGGTGATGAAGGTGGTTTTGCGCCAAACCTAGGCT
CTAATGAAGAAGCACTACAAACTATCGTAGAAGCAATTGAAGCTGCTGGTTATAAGCCGGGTGAAGAAGTTAAACTTGCA
ATGGACGTAGCTGCTTCTGAAATCTACAGTGATGGTAAATATAACCTAAAAGGTGAGGGAGTTGTTCGTTCATCTGAGGA
AATGGTTGACTGGTATGAAGAAATGATTTCTAAATACCCAATTATCTCTATTGAAGATGGATTGGATGAAAATGATTGGG
ATGGTTTCAAAATCCTAACTGATCGTCTTGGTGATAAAGTTCAATTAGTTGGAGATGACTTGTTCGTAACCAACACGAAT
AAACTTTCTAAAGGTATCGATCAAGGTATTGGTAACTCTATCTTAATCAAAGTAAACCAAATCGGAACTCTAACAGAAAC
ATTTGAAGCAATTGAAATGGCAAAACGCGCTGGTTATACAGCTGTTATCTCTCACCGTTCTGGTGAAACAGAAGATGTTA
CGATTGCAGATATTGCAGTAGCAACAAATGCTGGACAAATCAAAACAGGTGCACCATCTCGTACGGACCGTGTAGCTAAA
TATAACCAACTACTTCGCATTGAAGATGAATTAGCTGGTATGGGTGAGTATGGTGGATTAGCTTCATTCTACAACTTAGC
TAACAAATAA

Upstream 100 bases:

>100_bases
TATCACCAACACTGCTTGATTTATTAAATGTTGAAAAACCAAAAGAAATGACTGGCAATAGTCTTATAAAAAAATAATAA
AACAATAAGGAGAGAATAAT

Downstream 100 bases:

>100_bases
TTATAATGTCAATGATATAGTCAAAAACTACAATTGGAGTTATTACTCTGATTGTAGTTTTTTTATATACTTCATATCTA
ATTTATGAAAGTTTGCTACT

Product: enolase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MPYITDVYAREVLDSRGNPTIEVEIFTESGAFGSAIVPSGASTGEYEAVELRDGDKDRYLGKGVQKAVENVNDLIAPELI
GIDVTRQNIIDALMIDLDGTENKGKLGANAILGVSMAAAHAAANYLEVPLYNYLGGFNAKTLPTPMMNILNGGEHADNNV
DIQEFMIMPVGAPTFKEALRTGAEIFHALKKVLTSKGYNTAVGDEGGFAPNLGSNEEALQTIVEAIEAAGYKPGEEVKLA
MDVAASEIYSDGKYNLKGEGVVRSSEEMVDWYEEMISKYPIISIEDGLDENDWDGFKILTDRLGDKVQLVGDDLFVTNTN
KLSKGIDQGIGNSILIKVNQIGTLTETFEAIEMAKRAGYTAVISHRSGETEDVTIADIAVATNAGQIKTGAPSRTDRVAK
YNQLLRIEDELAGMGEYGGLASFYNLANK

Sequences:

>Translated_429_residues
MPYITDVYAREVLDSRGNPTIEVEIFTESGAFGSAIVPSGASTGEYEAVELRDGDKDRYLGKGVQKAVENVNDLIAPELI
GIDVTRQNIIDALMIDLDGTENKGKLGANAILGVSMAAAHAAANYLEVPLYNYLGGFNAKTLPTPMMNILNGGEHADNNV
DIQEFMIMPVGAPTFKEALRTGAEIFHALKKVLTSKGYNTAVGDEGGFAPNLGSNEEALQTIVEAIEAAGYKPGEEVKLA
MDVAASEIYSDGKYNLKGEGVVRSSEEMVDWYEEMISKYPIISIEDGLDENDWDGFKILTDRLGDKVQLVGDDLFVTNTN
KLSKGIDQGIGNSILIKVNQIGTLTETFEAIEMAKRAGYTAVISHRSGETEDVTIADIAVATNAGQIKTGAPSRTDRVAK
YNQLLRIEDELAGMGEYGGLASFYNLANK
>Mature_428_residues
PYITDVYAREVLDSRGNPTIEVEIFTESGAFGSAIVPSGASTGEYEAVELRDGDKDRYLGKGVQKAVENVNDLIAPELIG
IDVTRQNIIDALMIDLDGTENKGKLGANAILGVSMAAAHAAANYLEVPLYNYLGGFNAKTLPTPMMNILNGGEHADNNVD
IQEFMIMPVGAPTFKEALRTGAEIFHALKKVLTSKGYNTAVGDEGGFAPNLGSNEEALQTIVEAIEAAGYKPGEEVKLAM
DVAASEIYSDGKYNLKGEGVVRSSEEMVDWYEEMISKYPIISIEDGLDENDWDGFKILTDRLGDKVQLVGDDLFVTNTNK
LSKGIDQGIGNSILIKVNQIGTLTETFEAIEMAKRAGYTAVISHRSGETEDVTIADIAVATNAGQIKTGAPSRTDRVAKY
NQLLRIEDELAGMGEYGGLASFYNLANK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=51.4018691588785, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI4503571, Length=428, Percent_Identity=51.4018691588785, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=50.9345794392523, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=50.9345794392523, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=426, Percent_Identity=46.0093896713615, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI169201331, Length=347, Percent_Identity=26.8011527377522, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI169201757, Length=347, Percent_Identity=26.8011527377522, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI239744207, Length=347, Percent_Identity=26.8011527377522, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=65.0943396226415, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=52.0833333333333, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17536383, Length=432, Percent_Identity=52.0833333333333, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=46.3157894736842, Blast_Score=181, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=50.462962962963, Blast_Score=401, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=49.7685185185185, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=49.7685185185185, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=49.7685185185185, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=50, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=429, Percent_Identity=50.8158508158508, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580916, Length=429, Percent_Identity=50.8158508158508, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580920, Length=429, Percent_Identity=50.8158508158508, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580914, Length=429, Percent_Identity=50.8158508158508, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=50.8158508158508, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=50.8158508158508, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_OCEIH (Q8ENP5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693355.1
- ProteinModelPortal:   Q8ENP5
- SMR:   Q8ENP5
- GeneID:   1015437
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2434
- NMPDR:   fig|221109.1.peg.2431
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- BioCyc:   OIHE221109:OB2434-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46173; Mature: 46042

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYITDVYAREVLDSRGNPTIEVEIFTESGAFGSAIVPSGASTGEYEAVELRDGDKDRYL
CCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
GKGVQKAVENVNDLIAPELIGIDVTRQNIIDALMIDLDGTENKGKLGANAILGVSMAAAH
HHHHHHHHHHHHHHHCCHHEEECCHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHH
AAANYLEVPLYNYLGGFNAKTLPTPMMNILNGGEHADNNVDIQEFMIMPVGAPTFKEALR
HHHHHHCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHHHH
TGAEIFHALKKVLTSKGYNTAVGDEGGFAPNLGSNEEALQTIVEAIEAAGYKPGEEVKLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEHH
MDVAASEIYSDGKYNLKGEGVVRSSEEMVDWYEEMISKYPIISIEDGLDENDWDGFKILT
HHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHH
DRLGDKVQLVGDDLFVTNTNKLSKGIDQGIGNSILIKVNQIGTLTETFEAIEMAKRAGYT
HHCCCEEEEECCEEEEECCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDVTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDELAGMGEYGGL
EEEECCCCCCCCEEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHEEHHHHCCCCCCCCH
ASFYNLANK
HHHHHHCCC
>Mature Secondary Structure 
PYITDVYAREVLDSRGNPTIEVEIFTESGAFGSAIVPSGASTGEYEAVELRDGDKDRYL
CCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHH
GKGVQKAVENVNDLIAPELIGIDVTRQNIIDALMIDLDGTENKGKLGANAILGVSMAAAH
HHHHHHHHHHHHHHHCCHHEEECCHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHH
AAANYLEVPLYNYLGGFNAKTLPTPMMNILNGGEHADNNVDIQEFMIMPVGAPTFKEALR
HHHHHHCCCHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHHHH
TGAEIFHALKKVLTSKGYNTAVGDEGGFAPNLGSNEEALQTIVEAIEAAGYKPGEEVKLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEHH
MDVAASEIYSDGKYNLKGEGVVRSSEEMVDWYEEMISKYPIISIEDGLDENDWDGFKILT
HHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHH
DRLGDKVQLVGDDLFVTNTNKLSKGIDQGIGNSILIKVNQIGTLTETFEAIEMAKRAGYT
HHCCCEEEEECCEEEEECCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCE
AVISHRSGETEDVTIADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEDELAGMGEYGGL
EEEECCCCCCCCEEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHEEHHHHCCCCCCCCH
ASFYNLANK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376