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The map label for this gene is rihA [H]

Identifier: 23099888

GI number: 23099888

Start: 2487430

End: 2488413

Strand: Reverse

Name: rihA [H]

Synonym: OB2433

Alternate gene names: 23099888

Gene position: 2488413-2487430 (Counterclockwise)

Preceding gene: 23099889

Following gene: 23099885

Centisome position: 68.54

GC content: 32.83

Gene sequence:

>984_bases
ATGGTAAAGAAAGTATTGTTATTTGGTGATATAGGTATTGATGATATTATTACGCTCATTTATGCCGCATTAGATGAAGA
AATAGAAGTTATTGCAGTAGTCGCATCATATGGAAATATAAGTCGTGAGAAAGCCACAAATACAATTAGTTACATACAAG
ATCTATTAAATTATAAAAATCTACAGGTACCATTAATATTGGGGGCACAACGTCCAATGACGGGGGAAATACCAAAAGAA
GTATCGGATATTCATGGTGAGTACGGTATGGGCCCGATTATTCCTCCTATAAATGATGCGGAGGTTGTTGAAAACTTTTT
ACAGATAATCGATATAATTGAAAAAAATGAAGATATATACATAGCTAATATTGGCAGACTAACCGATTTAGCAACATTAT
TTATATTATATGAAGATTTAATGGGAAAAGTAAAAGGAATTTATATTATGGGGGGAGCATTTTGGGTTCCTGGAAATATT
ACAGCTGTAGCTGAAGCTAATTTTCACGGGGATCCAGTAGCCGCACAATTAGTGATGCGCTTTGCTTCTAACCTGAAGAT
TTTACCACTTAATGTATCAAATCAAGCAATAGTTACTCCTGAAATGGTAAATTATATTGATCAGGTCGGACAAATTTCTA
TTGTAAAGCCAATGATTGATTATTACTATGATTTTTATAAAAAAAGAGACCCATCAATTATAGGAAGTCCCATGCATGAT
GTATTAGTATTAATGGCAATAAAAAATAAAGATATGTTTCAATTTTTTAGTAGACCGATTACGATTGTCCAGTCATTGTT
AGGTGAGGAGAGGGGACAAAGTATTGCCGATATACGCTCCTATGAAGATGGATATTATACTATTACGGAGACGAATCACC
ATCAAATAGCTTTTCAAATGGATTATCAGCAATTCTTTTTCGATTTTATGACAAATATGTCGAATGGTCAATTTGATCGG
TCGTTATATAAAAAAGAAAAATAG

Upstream 100 bases:

>100_bases
AATTGGAGTTATTACTCTGATTGTAGTTTTTTTATATACTTCATATCTAATTTATGAAAGTTTGCTACTTATAAGCATAA
GCTAAACAAAGGGGGTAATT

Downstream 100 bases:

>100_bases
GTTCTCACTTGTATGATGAGAACCTATCAGTGTTAATCTTTTATTCCGTTATTTCCTTAACAAATGCTACTACTTCTTCT
GAAGTGCTCATTGCTAAGAA

Product: purine nucleoside hydrolase

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MVKKVLLFGDIGIDDIITLIYAALDEEIEVIAVVASYGNISREKATNTISYIQDLLNYKNLQVPLILGAQRPMTGEIPKE
VSDIHGEYGMGPIIPPINDAEVVENFLQIIDIIEKNEDIYIANIGRLTDLATLFILYEDLMGKVKGIYIMGGAFWVPGNI
TAVAEANFHGDPVAAQLVMRFASNLKILPLNVSNQAIVTPEMVNYIDQVGQISIVKPMIDYYYDFYKKRDPSIIGSPMHD
VLVLMAIKNKDMFQFFSRPITIVQSLLGEERGQSIADIRSYEDGYYTITETNHHQIAFQMDYQQFFFDFMTNMSNGQFDR
SLYKKEK

Sequences:

>Translated_327_residues
MVKKVLLFGDIGIDDIITLIYAALDEEIEVIAVVASYGNISREKATNTISYIQDLLNYKNLQVPLILGAQRPMTGEIPKE
VSDIHGEYGMGPIIPPINDAEVVENFLQIIDIIEKNEDIYIANIGRLTDLATLFILYEDLMGKVKGIYIMGGAFWVPGNI
TAVAEANFHGDPVAAQLVMRFASNLKILPLNVSNQAIVTPEMVNYIDQVGQISIVKPMIDYYYDFYKKRDPSIIGSPMHD
VLVLMAIKNKDMFQFFSRPITIVQSLLGEERGQSIADIRSYEDGYYTITETNHHQIAFQMDYQQFFFDFMTNMSNGQFDR
SLYKKEK
>Mature_327_residues
MVKKVLLFGDIGIDDIITLIYAALDEEIEVIAVVASYGNISREKATNTISYIQDLLNYKNLQVPLILGAQRPMTGEIPKE
VSDIHGEYGMGPIIPPINDAEVVENFLQIIDIIEKNEDIYIANIGRLTDLATLFILYEDLMGKVKGIYIMGGAFWVPGNI
TAVAEANFHGDPVAAQLVMRFASNLKILPLNVSNQAIVTPEMVNYIDQVGQISIVKPMIDYYYDFYKKRDPSIIGSPMHD
VLVLMAIKNKDMFQFFSRPITIVQSLLGEERGQSIADIRSYEDGYYTITETNHHQIAFQMDYQQFFFDFMTNMSNGQFDR
SLYKKEK

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=313, Percent_Identity=27.7955271565495, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786213, Length=283, Percent_Identity=28.2685512367491, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788486, Length=260, Percent_Identity=28.4615384615385, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17565698, Length=193, Percent_Identity=31.6062176165803, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32565474, Length=177, Percent_Identity=29.3785310734463, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320608, Length=259, Percent_Identity=29.3436293436293, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 37057; Mature: 37057

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKVLLFGDIGIDDIITLIYAALDEEIEVIAVVASYGNISREKATNTISYIQDLLNYKN
CCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
LQVPLILGAQRPMTGEIPKEVSDIHGEYGMGPIIPPINDAEVVENFLQIIDIIEKNEDIY
CEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
IANIGRLTDLATLFILYEDLMGKVKGIYIMGGAFWVPGNITAVAEANFHGDPVAAQLVMR
EEECCHHHHHHHHHHHHHHHHHHHCEEEEECCEEECCCCEEEEEECCCCCCHHHHHHHHH
FASNLKILPLNVSNQAIVTPEMVNYIDQVGQISIVKPMIDYYYDFYKKRDPSIIGSPMHD
HHCCCEEEEECCCCCEEECHHHHHHHHHHCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHH
VLVLMAIKNKDMFQFFSRPITIVQSLLGEERGQSIADIRSYEDGYYTITETNHHQIAFQM
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCEEEEEEE
DYQQFFFDFMTNMSNGQFDRSLYKKEK
CHHHHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MVKKVLLFGDIGIDDIITLIYAALDEEIEVIAVVASYGNISREKATNTISYIQDLLNYKN
CCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
LQVPLILGAQRPMTGEIPKEVSDIHGEYGMGPIIPPINDAEVVENFLQIIDIIEKNEDIY
CEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
IANIGRLTDLATLFILYEDLMGKVKGIYIMGGAFWVPGNITAVAEANFHGDPVAAQLVMR
EEECCHHHHHHHHHHHHHHHHHHHCEEEEECCEEECCCCEEEEEECCCCCCHHHHHHHHH
FASNLKILPLNVSNQAIVTPEMVNYIDQVGQISIVKPMIDYYYDFYKKRDPSIIGSPMHD
HHCCCEEEEECCCCCEEECHHHHHHHHHHCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHH
VLVLMAIKNKDMFQFFSRPITIVQSLLGEERGQSIADIRSYEDGYYTITETNHHQIAFQM
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCEEEEEEE
DYQQFFFDFMTNMSNGQFDRSLYKKEK
CHHHHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA