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The map label for this gene is cwlO [H]

Identifier: 23099858

GI number: 23099858

Start: 2455678

End: 2456904

Strand: Reverse

Name: cwlO [H]

Synonym: OB2403

Alternate gene names: 23099858

Gene position: 2456904-2455678 (Counterclockwise)

Preceding gene: 23099859

Following gene: 23099857

Centisome position: 67.67

GC content: 36.43

Gene sequence:

>1227_bases
ATGTTGAAAAAGGTAATATTTACATTAACTACAGCAACTGTAGTTGGGTTAAGCAGTACTTTATTCACAGGTGTCGCACA
AGCAGAAACAGTTGAGGATTTAGAATCACAACAATCACAAATTCAAGAAGAAAGAGATGCTATTAAAGCAGATTTATCTG
ATGCTGAAAGTGAAATAGCAGATGTGCTTATTGAGTTAGAAGAACTTAATAAAGAGATCGAGCGTGTAGATAACGCGCTA
AAAGAAAATGAAGAAAAAATGGATGAAACACAAGATAAGATTGCTGATCGAGAAGCTGAAGTTGAGAAACTAGAAGAAGA
TATTGCAGAGTTAGAAGAAGCAATTGAAAAGCGTTATGAAATTCTAAAAGAGCGAGCTATATCTTTACAGCAAAGTGGTG
GGGAAATTACATACCTTGAAGTAATCTTTGGAGCTCAAGACTTTGGTGATTTCATTACACGTGTACAAGCTGTTAATAAA
ATTACTGATTCAGATACTTCTTTAATTGAACAACAAGAAGCAGATAAACAAGAAGTCGAAACAAAACAAGATGAAGTAGT
TTCTAAACTTGATGATTTAAAAGATATGGAAGTAGAAATTAAAGGAATGCTTGCAACAATTGAGGAACAAAAATCAGAGA
ATGATGTGAAGAAAGACGACTTGAAAGAAAAAGAAAACAACCTAGTGGCAATGAAAGATGAATTAGAAGTAGAAGACTCT
AGATTGGCTACTCTTGAAAAGGATGTTGAAAGAAGTTTAAATACTGCTACTTCTCCTGCGCCAAATACAGCAGTAGCAAG
TGCTAGTGTTGGCTCGAATTCAGAATCAGATAACAATAATGTTACAAGAACTTCAGGATCTGGTGGAGTAAGTACAGCGA
TTAATGCTGGATTTAATCATTTAGGAACACCATATGTTTGGGGTGGGAAAACACCAAGCGGATTTGATTGTTCTGGATTT
GTTTCTTGGGCATTCGCGCAAGGTGGAATTTCAATACCATCAAGCACCTCTGCTTTAGCAAGCACGGGATCGAAAGTATC
TGCTAGCAATATGCAGCCTGGAGATATCGTATTCTTTGATACGTATAAAACAAATGGTCACGTAGGTATCTACCTAGGTG
GAGGAGAATTTATTGGTGCACAAAATTCAACTGGACTTGCAGTAGCTAACATGACAAGTGGTTACTGGGCAGATAAATTT
AGTGGACATGTACGTAGTGTGCGTTAA

Upstream 100 bases:

>100_bases
TACATAATGTGTTCAAATATATAAAACTGCATAGGACTTTCTCACATTAATACATAAATAGTCTTATAGAATTGCAGTAC
AATAGGGAAGCGAGGAATAA

Downstream 100 bases:

>100_bases
TTAATTGATAAGAAGTGAAAAAACCATAAGGGAAAAATCCCTTATGGTTTTTTTCTTGTCAACGCAAATAAATGTATGAT
TTAGTTCTACCAATAAATTA

Product: hypothetical protein

Products: NA

Alternate protein names: D-gamma-glutamyl-meso-diaminopimelyl DL-endopeptidase; PSPA2 [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MLKKVIFTLTTATVVGLSSTLFTGVAQAETVEDLESQQSQIQEERDAIKADLSDAESEIADVLIELEELNKEIERVDNAL
KENEEKMDETQDKIADREAEVEKLEEDIAELEEAIEKRYEILKERAISLQQSGGEITYLEVIFGAQDFGDFITRVQAVNK
ITDSDTSLIEQQEADKQEVETKQDEVVSKLDDLKDMEVEIKGMLATIEEQKSENDVKKDDLKEKENNLVAMKDELEVEDS
RLATLEKDVERSLNTATSPAPNTAVASASVGSNSESDNNNVTRTSGSGGVSTAINAGFNHLGTPYVWGGKTPSGFDCSGF
VSWAFAQGGISIPSSTSALASTGSKVSASNMQPGDIVFFDTYKTNGHVGIYLGGGEFIGAQNSTGLAVANMTSGYWADKF
SGHVRSVR

Sequences:

>Translated_408_residues
MLKKVIFTLTTATVVGLSSTLFTGVAQAETVEDLESQQSQIQEERDAIKADLSDAESEIADVLIELEELNKEIERVDNAL
KENEEKMDETQDKIADREAEVEKLEEDIAELEEAIEKRYEILKERAISLQQSGGEITYLEVIFGAQDFGDFITRVQAVNK
ITDSDTSLIEQQEADKQEVETKQDEVVSKLDDLKDMEVEIKGMLATIEEQKSENDVKKDDLKEKENNLVAMKDELEVEDS
RLATLEKDVERSLNTATSPAPNTAVASASVGSNSESDNNNVTRTSGSGGVSTAINAGFNHLGTPYVWGGKTPSGFDCSGF
VSWAFAQGGISIPSSTSALASTGSKVSASNMQPGDIVFFDTYKTNGHVGIYLGGGEFIGAQNSTGLAVANMTSGYWADKF
SGHVRSVR
>Mature_408_residues
MLKKVIFTLTTATVVGLSSTLFTGVAQAETVEDLESQQSQIQEERDAIKADLSDAESEIADVLIELEELNKEIERVDNAL
KENEEKMDETQDKIADREAEVEKLEEDIAELEEAIEKRYEILKERAISLQQSGGEITYLEVIFGAQDFGDFITRVQAVNK
ITDSDTSLIEQQEADKQEVETKQDEVVSKLDDLKDMEVEIKGMLATIEEQKSENDVKKDDLKEKENNLVAMKDELEVEDS
RLATLEKDVERSLNTATSPAPNTAVASASVGSNSESDNNNVTRTSGSGGVSTAINAGFNHLGTPYVWGGKTPSGFDCSGF
VSWAFAQGGISIPSSTSALASTGSKVSASNMQPGDIVFFDTYKTNGHVGIYLGGGEFIGAQNSTGLAVANMTSGYWADKF
SGHVRSVR

Specific function: The C-terminal part of CwlO shows a cell wall hydrolytic DL-endopeptidase activity [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted. Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C40 family [H]

Homologues:

Organism=Escherichia coli, GI1788501, Length=100, Percent_Identity=41, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787944, Length=121, Percent_Identity=34.7107438016529, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1786421, Length=109, Percent_Identity=32.1100917431193, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788001, Length=102, Percent_Identity=40.1960784313725, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 44299; Mature: 44299

Theoretical pI: Translated: 4.07; Mature: 4.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKVIFTLTTATVVGLSSTLFTGVAQAETVEDLESQQSQIQEERDAIKADLSDAESEIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVLIELEELNKEIERVDNALKENEEKMDETQDKIADREAEVEKLEEDIAELEEAIEKRYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKERAISLQQSGGEITYLEVIFGAQDFGDFITRVQAVNKITDSDTSLIEQQEADKQEVE
HHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHH
TKQDEVVSKLDDLKDMEVEIKGMLATIEEQKSENDVKKDDLKEKENNLVAMKDELEVEDS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCHH
RLATLEKDVERSLNTATSPAPNTAVASASVGSNSESDNNNVTRTSGSGGVSTAINAGFNH
HHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHCHHH
LGTPYVWGGKTPSGFDCSGFVSWAFAQGGISIPSSTSALASTGSKVSASNMQPGDIVFFD
CCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEE
TYKTNGHVGIYLGGGEFIGAQNSTGLAVANMTSGYWADKFSGHVRSVR
EECCCCEEEEEECCCEEECCCCCCCEEEEECCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MLKKVIFTLTTATVVGLSSTLFTGVAQAETVEDLESQQSQIQEERDAIKADLSDAESEIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVLIELEELNKEIERVDNALKENEEKMDETQDKIADREAEVEKLEEDIAELEEAIEKRYE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKERAISLQQSGGEITYLEVIFGAQDFGDFITRVQAVNKITDSDTSLIEQQEADKQEVE
HHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHH
TKQDEVVSKLDDLKDMEVEIKGMLATIEEQKSENDVKKDDLKEKENNLVAMKDELEVEDS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEEECCCCCCHH
RLATLEKDVERSLNTATSPAPNTAVASASVGSNSESDNNNVTRTSGSGGVSTAINAGFNH
HHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCHHHHHHCHHH
LGTPYVWGGKTPSGFDCSGFVSWAFAQGGISIPSSTSALASTGSKVSASNMQPGDIVFFD
CCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEE
TYKTNGHVGIYLGGGEFIGAQNSTGLAVANMTSGYWADKFSGHVRSVR
EECCCCEEEEEECCCEEECCCCCCCEEEEECCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 3145906 [H]