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The map label for this gene is aceA [H]

Identifier: 23099859

GI number: 23099859

Start: 2457150

End: 2458436

Strand: Reverse

Name: aceA [H]

Synonym: OB2404

Alternate gene names: 23099859

Gene position: 2458436-2457150 (Counterclockwise)

Preceding gene: 23099860

Following gene: 23099858

Centisome position: 67.72

GC content: 38.62

Gene sequence:

>1287_bases
ATGAAAAATGAAAGAGTAGAGAATTTAACTAAAGAGTGGCAAGATGATATTCGTTGGGTGGGGATTGAACGTCCGTATAA
AGCAGAAGATGTAATAAGACTCAGAGGTTCTATTGATATAACGCATACTTTAGCAACTCGAGGAGCCTCGAAATTATGGA
AACTGATTAATGAAGAAGACTATGTAAATGCTTTAGGAGCACTGACCGGAAATCAAGCAGTTCAACAAGTGAAAGCGGGA
CTTAAAGCAATATATTTAAGCGGGTGGCAGGTTGCTGCTGATGCAAATATGGCAGGAGAAATGTATCCTGATCAAAGTCT
ATACCCTGCAAATAGTGTCCCTCAGGTGGTGAGAAGAATTAATCAAGCACTACAACGATCGGATCAGATTCATTTTTCAG
AAGGGGAGAATGATATTGATTGGTTCGCTCCGATAGTTGCTGATGCAGAAGCTGGTTTTGGAGGTCAACTAAATGTATTC
GAATTAATGAAATCAATGATTGAAGCGGGTGCTGCTGCTGTCCATTTTGAAGATCAATTATCATCTGAAAAGAAGTGCGG
TCATCTAGGTGGTAAAGTGTTGCTGCCAACACAGAATGCAGTCCGAAATTTAATCGCAGCCCGATTTGCTGCAGATGTAA
TGGGTACCCCAACTATAATCATCGCACGCACAGATGCAAACGCAGCAGATATGATAACTAGTGATGTAGATGAATACGAT
CATTTATTTATCACTGGAGAAAGAACTGTAGAAGGGTTCTACAAAACAAAGGCAGGAATCGATCAGGCAATAGCTAGAGG
GCTAGCATATGCACCTTATGCTGATTTAATTTGGTGCGAAACATCGGAGCCAAATTTGGAGGAAGCTAGAATATTTGCAG
AAGCTATACATGAAAAGTTTCCAAATAAATTACTCGCATATAATTGTTCACCATCATTTAATTGGAAGAAAAAACTCTCA
GATCAAGAAATAGCAGAGTTCCAAAAAGAGCTAGGTAAATTAGGTTATAAATTCCAATTTGTAACATTAGCGGGTTTTCA
TGCATTAAATCACAGTATGTTCGATTTAGCTAAAAAGTATAAAAGTCATGGTATGGCAGCTTATTCTGAATTACAGCAAG
CAGAATTTTCAAGTGAAAAAGACGGATATACAGCAACTAGGCATCAGAGAGAAGTTGGTACAGGTTATTTTGACGAAGTT
GCGCAAGTCATTTCTGGTGGCAAGGCTTCTACTGCGGCGTTAATAGGTTCCACTGAAACAGAACAATTTACAAGTTTTTA
CAAATAA

Upstream 100 bases:

>100_bases
AAGCTGCTACGTTATTTCTATCTTTAATCAAGCAAAAAGACTTTGAAGAATTTTTAACTTTACCAGCTTATCAACAATTA
AATGAGGGAGGAACTGTATA

Downstream 100 bases:

>100_bases
ACATGAAAAAATCTGTTCCCTAATGATAGGGAACAGATTTTTTTGACTGTCGTAGCAATTTTTGTCGAAATTGAAATATA
ACTGTAACATTCCTATAATA

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKNERVENLTKEWQDDIRWVGIERPYKAEDVIRLRGSIDITHTLATRGASKLWKLINEEDYVNALGALTGNQAVQQVKAG
LKAIYLSGWQVAADANMAGEMYPDQSLYPANSVPQVVRRINQALQRSDQIHFSEGENDIDWFAPIVADAEAGFGGQLNVF
ELMKSMIEAGAAAVHFEDQLSSEKKCGHLGGKVLLPTQNAVRNLIAARFAADVMGTPTIIIARTDANAADMITSDVDEYD
HLFITGERTVEGFYKTKAGIDQAIARGLAYAPYADLIWCETSEPNLEEARIFAEAIHEKFPNKLLAYNCSPSFNWKKKLS
DQEIAEFQKELGKLGYKFQFVTLAGFHALNHSMFDLAKKYKSHGMAAYSELQQAEFSSEKDGYTATRHQREVGTGYFDEV
AQVISGGKASTAALIGSTETEQFTSFYK

Sequences:

>Translated_428_residues
MKNERVENLTKEWQDDIRWVGIERPYKAEDVIRLRGSIDITHTLATRGASKLWKLINEEDYVNALGALTGNQAVQQVKAG
LKAIYLSGWQVAADANMAGEMYPDQSLYPANSVPQVVRRINQALQRSDQIHFSEGENDIDWFAPIVADAEAGFGGQLNVF
ELMKSMIEAGAAAVHFEDQLSSEKKCGHLGGKVLLPTQNAVRNLIAARFAADVMGTPTIIIARTDANAADMITSDVDEYD
HLFITGERTVEGFYKTKAGIDQAIARGLAYAPYADLIWCETSEPNLEEARIFAEAIHEKFPNKLLAYNCSPSFNWKKKLS
DQEIAEFQKELGKLGYKFQFVTLAGFHALNHSMFDLAKKYKSHGMAAYSELQQAEFSSEKDGYTATRHQREVGTGYFDEV
AQVISGGKASTAALIGSTETEQFTSFYK
>Mature_428_residues
MKNERVENLTKEWQDDIRWVGIERPYKAEDVIRLRGSIDITHTLATRGASKLWKLINEEDYVNALGALTGNQAVQQVKAG
LKAIYLSGWQVAADANMAGEMYPDQSLYPANSVPQVVRRINQALQRSDQIHFSEGENDIDWFAPIVADAEAGFGGQLNVF
ELMKSMIEAGAAAVHFEDQLSSEKKCGHLGGKVLLPTQNAVRNLIAARFAADVMGTPTIIIARTDANAADMITSDVDEYD
HLFITGERTVEGFYKTKAGIDQAIARGLAYAPYADLIWCETSEPNLEEARIFAEAIHEKFPNKLLAYNCSPSFNWKKKLS
DQEIAEFQKELGKLGYKFQFVTLAGFHALNHSMFDLAKKYKSHGMAAYSELQQAEFSSEKDGYTATRHQREVGTGYFDEV
AQVISGGKASTAALIGSTETEQFTSFYK

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=432, Percent_Identity=64.5833333333333, Blast_Score=584, Evalue=1e-168,
Organism=Escherichia coli, GI1786525, Length=262, Percent_Identity=27.8625954198473, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17561814, Length=415, Percent_Identity=63.6144578313253, Blast_Score=559, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71982926, Length=415, Percent_Identity=63.6144578313253, Blast_Score=558, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6320908, Length=313, Percent_Identity=35.4632587859425, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325263, Length=251, Percent_Identity=38.2470119521912, Blast_Score=167, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 47541; Mature: 47541

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNERVENLTKEWQDDIRWVGIERPYKAEDVIRLRGSIDITHTLATRGASKLWKLINEED
CCCHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHCCCCEEHHHHHHCCHHHHHHHHCHHH
YVNALGALTGNQAVQQVKAGLKAIYLSGWQVAADANMAGEMYPDQSLYPANSVPQVVRRI
HHHHHHHHCCHHHHHHHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
NQALQRSDQIHFSEGENDIDWFAPIVADAEAGFGGQLNVFELMKSMIEAGAAAVHFEDQL
HHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEHHHH
SSEKKCGHLGGKVLLPTQNAVRNLIAARFAADVMGTPTIIIARTDANAADMITSDVDEYD
CCHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCC
HLFITGERTVEGFYKTKAGIDQAIARGLAYAPYADLIWCETSEPNLEEARIFAEAIHEKF
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHC
PNKLLAYNCSPSFNWKKKLSDQEIAEFQKELGKLGYKFQFVTLAGFHALNHSMFDLAKKY
CCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHH
KSHGMAAYSELQQAEFSSEKDGYTATRHQREVGTGYFDEVAQVISGGKASTAALIGSTET
HHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCH
EQFTSFYK
HHHHHHCC
>Mature Secondary Structure
MKNERVENLTKEWQDDIRWVGIERPYKAEDVIRLRGSIDITHTLATRGASKLWKLINEED
CCCHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHCCCCEEHHHHHHCCHHHHHHHHCHHH
YVNALGALTGNQAVQQVKAGLKAIYLSGWQVAADANMAGEMYPDQSLYPANSVPQVVRRI
HHHHHHHHCCHHHHHHHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
NQALQRSDQIHFSEGENDIDWFAPIVADAEAGFGGQLNVFELMKSMIEAGAAAVHFEDQL
HHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEEEEHHHH
SSEKKCGHLGGKVLLPTQNAVRNLIAARFAADVMGTPTIIIARTDANAADMITSDVDEYD
CCHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCC
HLFITGERTVEGFYKTKAGIDQAIARGLAYAPYADLIWCETSEPNLEEARIFAEAIHEKF
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHC
PNKLLAYNCSPSFNWKKKLSDQEIAEFQKELGKLGYKFQFVTLAGFHALNHSMFDLAKKY
CCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHH
KSHGMAAYSELQQAEFSSEKDGYTATRHQREVGTGYFDEVAQVISGGKASTAALIGSTET
HHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCCH
EQFTSFYK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]