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The map label for this gene is opuD [H]

Identifier: 23099857

GI number: 23099857

Start: 2453995

End: 2455485

Strand: Reverse

Name: opuD [H]

Synonym: OB2402

Alternate gene names: 23099857

Gene position: 2455485-2453995 (Counterclockwise)

Preceding gene: 23099858

Following gene: 23099856

Centisome position: 67.63

GC content: 36.08

Gene sequence:

>1491_bases
ATGAATAAACATAAGTTTTTAAACCCAGTATTTTTTGTTTCGTCTATATTCATATTAATACTAGTTGTCATAGGAGCTAT
AAGCCCTGGCAGATTTGAGGCAGTGGCTAATAAATTATTTAACTTTACTACTATTAATTTTGCATGGTTTTACCTACTTG
CAGTTTTTGTATTCATCCTTTTTTTAATTTGGTTGGCTTTCGGTAAGTATGGCAAATTAAAACTTGGACCTGCTGATTCA
AAACCCGAGTATTCTTTTTTTGCTTGGGTTGGAATGCTATTTTCAACAGGATTTGGTTCTAGCCTCGTTTTCTGGGGTGT
TGCAGAGCCGATGACTCACTTTTTTAGTACGCCTTTTCCTGGGCTAGAAGCACAATCAGAAGAAGCTGCTCGTGTTGCTA
TGGGTTATTCTTTCTTTAACTGGGGAATTAGTCAGTGGGCGGTTTTTGCGTTAGTTGGTCTAGTGATAGCTTATCTTCAG
TTCCGTAAAGATAAAAATGGTTTGATATCAACAGCAATAGAGCCGGTAGTTGGAAAAAACCGCTTCACTGCTAACACGGT
AAATTCATTAGCTGTAATTGCGACTGTGATGGGAGTAGCTACTTCACTAGGATTTGGAATTATGCAAATGAATGGTGGGT
TAAATTCGGTATTTGGACTTTCAAATTCAATATGGGTACAAATAGGAATCGCGGTTGCTATGTTGCTTGTTTACCTGATA
TCATCTGCAACAGGTTTGAAAAAAGGTATGAAATGGCTTAGTACTTTAAATTTAACTTTATGTTTAGCTCTGTTATTATT
TGTATTTATTATGGGTCCAACTGTATTTATCTTAGAATCCTTTGTACTAGGGATTGGAGATTACATTACTAATTTTGTAC
AATATAGTTTGAGAATGACCCCATATACTGGAGAATCTTGGGTGACAGAATGGACGGTATTCTACTGGGCTTGGGCAATA
TCTTGGTCTCCATTTGTAGGGGCGTTTGTAGCTAGAGTATCGCGTGGTAGAACCATTCGTCAATTTATATTTGGTGTCAC
AGCAGTTTCACCCATAATTGCTTGTCTATGGATAGCTGTATTTGGCGGGACCGCATTATTTAGTGATTTAAATAATGGTA
CCATGATAGCAGAAGCAGTAGATGAGGATTTAGCAGTAGCCTTATTTGAGACATTTGGTACCTTACCATTAAGTACAATT
ATGTCTTTATTAGCTATTTTTCTTATATTTACTTTCCTAGTAACTTCAGCGGACTCGGCTACTTATATTTTGGGTAGTAT
GACTTCAAAAGGAAGTTTGAATCCAGCACTTGTTACAAAGATTGTGTGGGGACTGTTAATTACTGCTATTGCAATTGTTC
TATTATTAGCTGGTGGCTTAGAAGCACTGCAAACGGCAGCGTTAACGTCAGCATTGCCATTTACAATAATAATAATTATT
TTAATGATTTCCTTCCTAAAAATAATAAAAAAAGATAGAAGTTCTAAATAG

Upstream 100 bases:

>100_bases
ATAAATTAGTATTAGGTAAATATGTGGTTTAAGTTTTTTAATAATCGGAAATATATAGTTATCGCTATCATACATAAAAA
AACAAAGAAAGGATTAACTA

Downstream 100 bases:

>100_bases
GTTAACTTTAGAGATTTTAAGGAACCCGGACATAAATCACTTTTCTTATACTAGTGATCCATGTTCGGGTTATCTTATTT
GAAGAAACGGTTATATGCTT

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MNKHKFLNPVFFVSSIFILILVVIGAISPGRFEAVANKLFNFTTINFAWFYLLAVFVFILFLIWLAFGKYGKLKLGPADS
KPEYSFFAWVGMLFSTGFGSSLVFWGVAEPMTHFFSTPFPGLEAQSEEAARVAMGYSFFNWGISQWAVFALVGLVIAYLQ
FRKDKNGLISTAIEPVVGKNRFTANTVNSLAVIATVMGVATSLGFGIMQMNGGLNSVFGLSNSIWVQIGIAVAMLLVYLI
SSATGLKKGMKWLSTLNLTLCLALLLFVFIMGPTVFILESFVLGIGDYITNFVQYSLRMTPYTGESWVTEWTVFYWAWAI
SWSPFVGAFVARVSRGRTIRQFIFGVTAVSPIIACLWIAVFGGTALFSDLNNGTMIAEAVDEDLAVALFETFGTLPLSTI
MSLLAIFLIFTFLVTSADSATYILGSMTSKGSLNPALVTKIVWGLLITAIAIVLLLAGGLEALQTAALTSALPFTIIIII
LMISFLKIIKKDRSSK

Sequences:

>Translated_496_residues
MNKHKFLNPVFFVSSIFILILVVIGAISPGRFEAVANKLFNFTTINFAWFYLLAVFVFILFLIWLAFGKYGKLKLGPADS
KPEYSFFAWVGMLFSTGFGSSLVFWGVAEPMTHFFSTPFPGLEAQSEEAARVAMGYSFFNWGISQWAVFALVGLVIAYLQ
FRKDKNGLISTAIEPVVGKNRFTANTVNSLAVIATVMGVATSLGFGIMQMNGGLNSVFGLSNSIWVQIGIAVAMLLVYLI
SSATGLKKGMKWLSTLNLTLCLALLLFVFIMGPTVFILESFVLGIGDYITNFVQYSLRMTPYTGESWVTEWTVFYWAWAI
SWSPFVGAFVARVSRGRTIRQFIFGVTAVSPIIACLWIAVFGGTALFSDLNNGTMIAEAVDEDLAVALFETFGTLPLSTI
MSLLAIFLIFTFLVTSADSATYILGSMTSKGSLNPALVTKIVWGLLITAIAIVLLLAGGLEALQTAALTSALPFTIIIII
LMISFLKIIKKDRSSK
>Mature_496_residues
MNKHKFLNPVFFVSSIFILILVVIGAISPGRFEAVANKLFNFTTINFAWFYLLAVFVFILFLIWLAFGKYGKLKLGPADS
KPEYSFFAWVGMLFSTGFGSSLVFWGVAEPMTHFFSTPFPGLEAQSEEAARVAMGYSFFNWGISQWAVFALVGLVIAYLQ
FRKDKNGLISTAIEPVVGKNRFTANTVNSLAVIATVMGVATSLGFGIMQMNGGLNSVFGLSNSIWVQIGIAVAMLLVYLI
SSATGLKKGMKWLSTLNLTLCLALLLFVFIMGPTVFILESFVLGIGDYITNFVQYSLRMTPYTGESWVTEWTVFYWAWAI
SWSPFVGAFVARVSRGRTIRQFIFGVTAVSPIIACLWIAVFGGTALFSDLNNGTMIAEAVDEDLAVALFETFGTLPLSTI
MSLLAIFLIFTFLVTSADSATYILGSMTSKGSLNPALVTKIVWGLLITAIAIVLLLAGGLEALQTAALTSALPFTIIIII
LMISFLKIIKKDRSSK

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=503, Percent_Identity=34.3936381709742, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1788102, Length=445, Percent_Identity=31.4606741573034, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1786224, Length=493, Percent_Identity=29.6146044624746, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 54221; Mature: 54221

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKHKFLNPVFFVSSIFILILVVIGAISPGRFEAVANKLFNFTTINFAWFYLLAVFVFIL
CCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLIWLAFGKYGKLKLGPADSKPEYSFFAWVGMLFSTGFGSSLVFWGVAEPMTHFFSTPFP
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
GLEAQSEEAARVAMGYSFFNWGISQWAVFALVGLVIAYLQFRKDKNGLISTAIEPVVGKN
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
RFTANTVNSLAVIATVMGVATSLGFGIMQMNGGLNSVFGLSNSIWVQIGIAVAMLLVYLI
CCCHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
SSATGLKKGMKWLSTLNLTLCLALLLFVFIMGPTVFILESFVLGIGDYITNFVQYSLRMT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
PYTGESWVTEWTVFYWAWAISWSPFVGAFVARVSRGRTIRQFIFGVTAVSPIIACLWIAV
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FGGTALFSDLNNGTMIAEAVDEDLAVALFETFGTLPLSTIMSLLAIFLIFTFLVTSADSA
HCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
TYILGSMTSKGSLNPALVTKIVWGLLITAIAIVLLLAGGLEALQTAALTSALPFTIIIII
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LMISFLKIIKKDRSSK
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNKHKFLNPVFFVSSIFILILVVIGAISPGRFEAVANKLFNFTTINFAWFYLLAVFVFIL
CCCCHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FLIWLAFGKYGKLKLGPADSKPEYSFFAWVGMLFSTGFGSSLVFWGVAEPMTHFFSTPFP
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
GLEAQSEEAARVAMGYSFFNWGISQWAVFALVGLVIAYLQFRKDKNGLISTAIEPVVGKN
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
RFTANTVNSLAVIATVMGVATSLGFGIMQMNGGLNSVFGLSNSIWVQIGIAVAMLLVYLI
CCCHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCHHHHCCCCHHHHHHHHHHHHHHHHHH
SSATGLKKGMKWLSTLNLTLCLALLLFVFIMGPTVFILESFVLGIGDYITNFVQYSLRMT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
PYTGESWVTEWTVFYWAWAISWSPFVGAFVARVSRGRTIRQFIFGVTAVSPIIACLWIAV
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
FGGTALFSDLNNGTMIAEAVDEDLAVALFETFGTLPLSTIMSLLAIFLIFTFLVTSADSA
HCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC
TYILGSMTSKGSLNPALVTKIVWGLLITAIAIVLLLAGGLEALQTAALTSALPFTIIIII
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LMISFLKIIKKDRSSK
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]