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The map label for this gene is licH [H]

Identifier: 23099728

GI number: 23099728

Start: 2316065

End: 2317375

Strand: Direct

Name: licH [H]

Synonym: OB2273

Alternate gene names: 23099728

Gene position: 2316065-2317375 (Clockwise)

Preceding gene: 23099725

Following gene: 23099729

Centisome position: 63.79

GC content: 36.0

Gene sequence:

>1311_bases
ATGGCATTAAAACTCGTAATTATCGGTGGTGGTTCAAGTTACACTCCTGAAATTCTAGAAGGGATTATTCAACGACAGGA
TACCTTTCCTGTAAATGAGATTGTTCTCGTTGATATTGAACCAGGTAAAGACAAACTAGAAACCATAGGAAATCTAGGGA
AACGGATGATTCATCAAGCGAATGCGAATATTAACCTTTCTTGGACATTGAACCGTAGAGAGGCATTAGTCAATGCAGAT
TTTATTACTACTCAAATTCGTGTTGGTGGCCTCCAAGCACGTTCAATAGATGAACGAATCCCCCTAAAACATGGTTTCAT
TGGACAGGAAACAAATGGCGCAGGCGGTATAATGAAAGCATTTCGAACGATACCAGTATTGATGGAGATTGCAGAAGATG
TACAAGAAATTTGCCCGGATGCATGGATTGTAAACTTCACAAACCCGGCAGGAATCGTTACAGAAGCATTATTAAATTAC
TCTAATCATAAAAAAGTCATAGGTGTTTGTAATATCCCTTTTAATACTCAGCATTCCACTGCCGAACTATTAGATGTGAA
ACCTGAAGAGGTACTAATTGAATTTATCGGACTGAACCACTTTATTTTTGGAAAGAAGGTGCTTGTATCCGGCAAAGACC
GAACGGAAGAAGTATTTGATCGTTTAATACATAGGGAAACCAATTATACACCTGCAAATATAATGCCGTTAGATTGGTCA
GAAACATTTATTCAATCAACAAAACTACTACCTAATCCTTATCATCAATATTATTTCCAAACAGAGACGGTTCTTGAAAA
AGATTTACAATCATATCAAGAAAATGGTACTCGAGCGGAAGTAGTACAAGAATTAGAAAAATCACTATTCGAATTATATC
AACAACCTAATTTAAAAACGAAACCAAAAGAACTTGAAAAAAGAGGTGGAGCGTTCTATAGCAAAGTTGCATGTAGTTTG
ATGGATTCCATCTACAATAATAAGAACGATGTACAAACAGTGAATACACAAAATAATGGTACGATTACTGATTTACCAGC
TGATGCAGTTATTGAAGTTAATTGTGTGATAACCAAAGATGGACCAAAACCGATTTCCATTGGTAAGATTCCTAGCCCAG
TAAAAGGGATCATCCAAAATATGAAAGCAATGGAAGACCTCACTATTCAGGCTGCTTTATCGGGAAATTATCAAGATGCC
TACAATGCGTTTGTTATGAATCCATTAATTCAAGACGAAAAAAAAGCAAAAGTGTTACTTGATGAACTTTTAGAAGCTCA
CAAAGCTTACCTACCACAATTTAAACAATAG

Upstream 100 bases:

>100_bases
TTTGTATGGACAATAAAAAAATTGTATGATACATTTTATTTAAGTTATCGAAACATTTTTAATGTTAGCGATTACATTAA
TGGTAAAGGAGCACTTAATA

Downstream 100 bases:

>100_bases
GAGGAAATAAGCATGAATAACAAATTTATGCAGAAATTCATTGAAATAGCTGGTCGTATTGGTTCACAAAGACATTTAGT
TGCCATACGTGATGGTTTCG

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MALKLVIIGGGSSYTPEILEGIIQRQDTFPVNEIVLVDIEPGKDKLETIGNLGKRMIHQANANINLSWTLNRREALVNAD
FITTQIRVGGLQARSIDERIPLKHGFIGQETNGAGGIMKAFRTIPVLMEIAEDVQEICPDAWIVNFTNPAGIVTEALLNY
SNHKKVIGVCNIPFNTQHSTAELLDVKPEEVLIEFIGLNHFIFGKKVLVSGKDRTEEVFDRLIHRETNYTPANIMPLDWS
ETFIQSTKLLPNPYHQYYFQTETVLEKDLQSYQENGTRAEVVQELEKSLFELYQQPNLKTKPKELEKRGGAFYSKVACSL
MDSIYNNKNDVQTVNTQNNGTITDLPADAVIEVNCVITKDGPKPISIGKIPSPVKGIIQNMKAMEDLTIQAALSGNYQDA
YNAFVMNPLIQDEKKAKVLLDELLEAHKAYLPQFKQ

Sequences:

>Translated_436_residues
MALKLVIIGGGSSYTPEILEGIIQRQDTFPVNEIVLVDIEPGKDKLETIGNLGKRMIHQANANINLSWTLNRREALVNAD
FITTQIRVGGLQARSIDERIPLKHGFIGQETNGAGGIMKAFRTIPVLMEIAEDVQEICPDAWIVNFTNPAGIVTEALLNY
SNHKKVIGVCNIPFNTQHSTAELLDVKPEEVLIEFIGLNHFIFGKKVLVSGKDRTEEVFDRLIHRETNYTPANIMPLDWS
ETFIQSTKLLPNPYHQYYFQTETVLEKDLQSYQENGTRAEVVQELEKSLFELYQQPNLKTKPKELEKRGGAFYSKVACSL
MDSIYNNKNDVQTVNTQNNGTITDLPADAVIEVNCVITKDGPKPISIGKIPSPVKGIIQNMKAMEDLTIQAALSGNYQDA
YNAFVMNPLIQDEKKAKVLLDELLEAHKAYLPQFKQ
>Mature_435_residues
ALKLVIIGGGSSYTPEILEGIIQRQDTFPVNEIVLVDIEPGKDKLETIGNLGKRMIHQANANINLSWTLNRREALVNADF
ITTQIRVGGLQARSIDERIPLKHGFIGQETNGAGGIMKAFRTIPVLMEIAEDVQEICPDAWIVNFTNPAGIVTEALLNYS
NHKKVIGVCNIPFNTQHSTAELLDVKPEEVLIEFIGLNHFIFGKKVLVSGKDRTEEVFDRLIHRETNYTPANIMPLDWSE
TFIQSTKLLPNPYHQYYFQTETVLEKDLQSYQENGTRAEVVQELEKSLFELYQQPNLKTKPKELEKRGGAFYSKVACSLM
DSIYNNKNDVQTVNTQNNGTITDLPADAVIEVNCVITKDGPKPISIGKIPSPVKGIIQNMKAMEDLTIQAALSGNYQDAY
NAFVMNPLIQDEKKAKVLLDELLEAHKAYLPQFKQ

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=435, Percent_Identity=47.3563218390805, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1790560, Length=470, Percent_Identity=25.7446808510638, Blast_Score=155, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 48901; Mature: 48769

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKLVIIGGGSSYTPEILEGIIQRQDTFPVNEIVLVDIEPGKDKLETIGNLGKRMIHQA
CEEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHC
NANINLSWTLNRREALVNADFITTQIRVGGLQARSIDERIPLKHGFIGQETNGAGGIMKA
CCCEEEEEEECHHHHHCCCCEEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHH
FRTIPVLMEIAEDVQEICPDAWIVNFTNPAGIVTEALLNYSNHKKVIGVCNIPFNTQHST
HHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCH
AELLDVKPEEVLIEFIGLNHFIFGKKVLVSGKDRTEEVFDRLIHRETNYTPANIMPLDWS
HHHHCCCHHHHHHHHHCCCEEEECCEEEECCCCCHHHHHHHHHHCCCCCCCCCEEECCCH
ETFIQSTKLLPNPYHQYYFQTETVLEKDLQSYQENGTRAEVVQELEKSLFELYQQPNLKT
HHHHHHHCCCCCHHHHHEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
KPKELEKRGGAFYSKVACSLMDSIYNNKNDVQTVNTQNNGTITDLPADAVIEVNCVITKD
CHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCEEEEEEEEECC
GPKPISIGKIPSPVKGIIQNMKAMEDLTIQAALSGNYQDAYNAFVMNPLIQDEKKAKVLL
CCCCEECCCCCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHCHHHCCHHHHHHHH
DELLEAHKAYLPQFKQ
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALKLVIIGGGSSYTPEILEGIIQRQDTFPVNEIVLVDIEPGKDKLETIGNLGKRMIHQA
EEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHC
NANINLSWTLNRREALVNADFITTQIRVGGLQARSIDERIPLKHGFIGQETNGAGGIMKA
CCCEEEEEEECHHHHHCCCCEEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHH
FRTIPVLMEIAEDVQEICPDAWIVNFTNPAGIVTEALLNYSNHKKVIGVCNIPFNTQHST
HHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCH
AELLDVKPEEVLIEFIGLNHFIFGKKVLVSGKDRTEEVFDRLIHRETNYTPANIMPLDWS
HHHHCCCHHHHHHHHHCCCEEEECCEEEECCCCCHHHHHHHHHHCCCCCCCCCEEECCCH
ETFIQSTKLLPNPYHQYYFQTETVLEKDLQSYQENGTRAEVVQELEKSLFELYQQPNLKT
HHHHHHHCCCCCHHHHHEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
KPKELEKRGGAFYSKVACSLMDSIYNNKNDVQTVNTQNNGTITDLPADAVIEVNCVITKD
CHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCEEEEEEEEECC
GPKPISIGKIPSPVKGIIQNMKAMEDLTIQAALSGNYQDAYNAFVMNPLIQDEKKAKVLL
CCCCEECCCCCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHCHHHCCHHHHHHHH
DELLEAHKAYLPQFKQ
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]