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The map label for this gene is prpD [H]

Identifier: 23099723

GI number: 23099723

Start: 2310665

End: 2312101

Strand: Reverse

Name: prpD [H]

Synonym: OB2268

Alternate gene names: 23099723

Gene position: 2312101-2310665 (Counterclockwise)

Preceding gene: 23099724

Following gene: 23099722

Centisome position: 63.68

GC content: 36.88

Gene sequence:

>1437_bases
ATGACACAAGTAAAAACGATAAATCAAACAGATAAAATTATTGAAGAAATAGCTGATTATGTAGTTCATACAGAAATTAC
GAGTGAAGAAGCTTATAGAACTGCACATCATGTATTAATTGATACACTCGGTTGTGGAATTTTAGCATTGAACTATCCGG
AATGTACTAAATTATTAGGACCGATTGTACCAGGTACAGTTGTACCTAATGGTAGTAGGGTTCCTGGTACCTCATATGTA
CTAGACCCAGTACAAGGTGCTTTTAATATTGGTACAATGATTCGTTGGTTAGACTACAATGACACTTGGCTTGCGGCAGA
ATGGGGACATCCTTCAGATAATCTAGGTGGAATCTTAGCTGTTGCAGATTATGTGAGCCAAGAAAGATTAAGTAAAGGTG
AAGAGCCAATAAAGGTAAAAGAAATACTTGAAATGATGATTAAAGCACATGAAATTCAGGGTGTATTAGCATTAGAAAAT
AGCTTAAATCGAGTTGGGTTAGATCATGTTTTATATGTAAAAATCGCTACTACGGCCGTTGTAACCAAAATGCTAGGGGG
AACCAAAGAAGAGATACAAAATGCGTTATCCAATGCTTGGATTGATAATTCAAGTTTACGTACGTATCGTCATTTTCCAA
ACACAGGTTCCAGAAAGTCCTGGGCTGCGGGAGATGCGACAAGTAGAGCAGTTCGATTAGCTTTAATGGCAATCAAAGGT
GAAATGGGATATGCAACAGCTCTTACTGCAAATGGTTGGGGATTCCAGGATGTTCTTTTTAAAGGACAAGAGCTGAAGTT
AAGTCGCTCTTTAGGAAGTTATGTTATGGAAAATGTCTTATTTAAAGTATCGTATCCTGCAGAATTCCATGCGCAAACTG
CAGCTGAAGCAGCTGTTCAATTACATCCAGAGATAATAGGACGTCTTGATGAAATTAAAGAAATAACGATTAAAACGCAT
GAATCTGCTATTCGAATTATTGATAAAACAGGTCCGTTACACAATCCAGCTGATCGAGATCATTGCTTGCAATATATTAC
CGCTATTGGTCTTTTAAAAGGAAATATTACTGCAGAAGATTATGAAGATGATGTGGCTGCTGATCCGCGTATTGATGAAT
TACGTGAAAAAATGGTGACTGTGGAGAAAGAATCTTATACAACGGATTATTTAGATCCTGAGAAGCGCTCCATTGCCAAT
GCAATACAAGTACACTTTGTAGACGGGACTTCTACAGATGTTATTGATTGTGAATATCCATTAGGGCATCGTTTCCGTCG
TGAGGAAGCAATACCTAAGATTTTGGAGAAGTATGCGCATAATTTGTCTACTCAATATACGGTTAAACAGAGTGAACGTA
TACAGAAGGTTACGAATAATTTTGATGAGGTACAACAAATGAATACAACGGATTTTGTTGATTTATTTGTAAATTAA

Upstream 100 bases:

>100_bases
AGCCCATATCGTTGAGCAACATTCAAACAATCGTATATTCCGTCCAAGAGTGAAATATATCGGAAAAGATTATTAAGGGA
TAAGAGAGGAGTTTTATTAT

Downstream 100 bases:

>100_bases
GGAGGACTTATAAATGGCTTGGATAGTAGACCAACCTATATCACAACAAGAGTTAGCAAGTCAGTTTCGATCCCAAGTAA
GTGAACAAGGAATTGCTCAA

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTQVKTINQTDKIIEEIADYVVHTEITSEEAYRTAHHVLIDTLGCGILALNYPECTKLLGPIVPGTVVPNGSRVPGTSYV
LDPVQGAFNIGTMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYVSQERLSKGEEPIKVKEILEMMIKAHEIQGVLALEN
SLNRVGLDHVLYVKIATTAVVTKMLGGTKEEIQNALSNAWIDNSSLRTYRHFPNTGSRKSWAAGDATSRAVRLALMAIKG
EMGYATALTANGWGFQDVLFKGQELKLSRSLGSYVMENVLFKVSYPAEFHAQTAAEAAVQLHPEIIGRLDEIKEITIKTH
ESAIRIIDKTGPLHNPADRDHCLQYITAIGLLKGNITAEDYEDDVAADPRIDELREKMVTVEKESYTTDYLDPEKRSIAN
AIQVHFVDGTSTDVIDCEYPLGHRFRREEAIPKILEKYAHNLSTQYTVKQSERIQKVTNNFDEVQQMNTTDFVDLFVN

Sequences:

>Translated_478_residues
MTQVKTINQTDKIIEEIADYVVHTEITSEEAYRTAHHVLIDTLGCGILALNYPECTKLLGPIVPGTVVPNGSRVPGTSYV
LDPVQGAFNIGTMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYVSQERLSKGEEPIKVKEILEMMIKAHEIQGVLALEN
SLNRVGLDHVLYVKIATTAVVTKMLGGTKEEIQNALSNAWIDNSSLRTYRHFPNTGSRKSWAAGDATSRAVRLALMAIKG
EMGYATALTANGWGFQDVLFKGQELKLSRSLGSYVMENVLFKVSYPAEFHAQTAAEAAVQLHPEIIGRLDEIKEITIKTH
ESAIRIIDKTGPLHNPADRDHCLQYITAIGLLKGNITAEDYEDDVAADPRIDELREKMVTVEKESYTTDYLDPEKRSIAN
AIQVHFVDGTSTDVIDCEYPLGHRFRREEAIPKILEKYAHNLSTQYTVKQSERIQKVTNNFDEVQQMNTTDFVDLFVN
>Mature_477_residues
TQVKTINQTDKIIEEIADYVVHTEITSEEAYRTAHHVLIDTLGCGILALNYPECTKLLGPIVPGTVVPNGSRVPGTSYVL
DPVQGAFNIGTMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYVSQERLSKGEEPIKVKEILEMMIKAHEIQGVLALENS
LNRVGLDHVLYVKIATTAVVTKMLGGTKEEIQNALSNAWIDNSSLRTYRHFPNTGSRKSWAAGDATSRAVRLALMAIKGE
MGYATALTANGWGFQDVLFKGQELKLSRSLGSYVMENVLFKVSYPAEFHAQTAAEAAVQLHPEIIGRLDEIKEITIKTHE
SAIRIIDKTGPLHNPADRDHCLQYITAIGLLKGNITAEDYEDDVAADPRIDELREKMVTVEKESYTTDYLDPEKRSIANA
IQVHFVDGTSTDVIDCEYPLGHRFRREEAIPKILEKYAHNLSTQYTVKQSERIQKVTNNFDEVQQMNTTDFVDLFVN

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=470, Percent_Identity=62.5531914893617, Blast_Score=627, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=480, Percent_Identity=54.1666666666667, Blast_Score=522, Evalue=1e-149,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 53363; Mature: 53232

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVKTINQTDKIIEEIADYVVHTEITSEEAYRTAHHVLIDTLGCGILALNYPECTKLLG
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHC
PIVPGTVVPNGSRVPGTSYVLDPVQGAFNIGTMIRWLDYNDTWLAAEWGHPSDNLGGILA
CCCCCCCCCCCCCCCCCCEEECCCCCHHHHCCEEEEECCCCCEEEEECCCCCCCCCHHHH
VADYVSQERLSKGEEPIKVKEILEMMIKAHEIQGVLALENSLNRVGLDHVLYVKIATTAV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCHHHCCCCEEEEEEHHHHHH
VTKMLGGTKEEIQNALSNAWIDNSSLRTYRHFPNTGSRKSWAAGDATSRAVRLALMAIKG
HHHHHCCCHHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
EMGYATALTANGWGFQDVLFKGQELKLSRSLGSYVMENVLFKVSYPAEFHAQTAAEAAVQ
CCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHH
LHPEIIGRLDEIKEITIKTHESAIRIIDKTGPLHNPADRDHCLQYITAIGLLKGNITAED
HCHHHHHHHHHHHHHHEEECCHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
YEDDVAADPRIDELREKMVTVEKESYTTDYLDPEKRSIANAIQVHFVDGTSTDVIDCEYP
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCEEEEEEECCCCCCEEECCCC
LGHRFRREEAIPKILEKYAHNLSTQYTVKQSERIQKVTNNFDEVQQMNTTDFVDLFVN
CCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHCCCCHHEEEEEC
>Mature Secondary Structure 
TQVKTINQTDKIIEEIADYVVHTEITSEEAYRTAHHVLIDTLGCGILALNYPECTKLLG
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHC
PIVPGTVVPNGSRVPGTSYVLDPVQGAFNIGTMIRWLDYNDTWLAAEWGHPSDNLGGILA
CCCCCCCCCCCCCCCCCCEEECCCCCHHHHCCEEEEECCCCCEEEEECCCCCCCCCHHHH
VADYVSQERLSKGEEPIKVKEILEMMIKAHEIQGVLALENSLNRVGLDHVLYVKIATTAV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCHHHCCCCEEEEEEHHHHHH
VTKMLGGTKEEIQNALSNAWIDNSSLRTYRHFPNTGSRKSWAAGDATSRAVRLALMAIKG
HHHHHCCCHHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
EMGYATALTANGWGFQDVLFKGQELKLSRSLGSYVMENVLFKVSYPAEFHAQTAAEAAVQ
CCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHH
LHPEIIGRLDEIKEITIKTHESAIRIIDKTGPLHNPADRDHCLQYITAIGLLKGNITAED
HCHHHHHHHHHHHHHHEEECCHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
YEDDVAADPRIDELREKMVTVEKESYTTDYLDPEKRSIANAIQVHFVDGTSTDVIDCEYP
CCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCEEEEEEECCCCCCEEECCCC
LGHRFRREEAIPKILEKYAHNLSTQYTVKQSERIQKVTNNFDEVQQMNTTDFVDLFVN
CCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHCCCCHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838 [H]