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The map label for this gene is prpB [H]

Identifier: 23099722

GI number: 23099722

Start: 2309746

End: 2310651

Strand: Reverse

Name: prpB [H]

Synonym: OB2267

Alternate gene names: 23099722

Gene position: 2310651-2309746 (Counterclockwise)

Preceding gene: 23099723

Following gene: 23099720

Centisome position: 63.65

GC content: 38.52

Gene sequence:

>906_bases
ATGGCTTGGATAGTAGACCAACCTATATCACAACAAGAGTTAGCAAGTCAGTTTCGATCCCAAGTAAGTGAACAAGGAAT
TGCTCAAATTCCTGGTGCACATGATGCGATGGCCGGACTGATTGCCAAAGAAGCAGGATTTCAATCTCTTTATCTTTCAG
GAGCAGCATATACCGCAAGTCGTGGCTTACCGGATTTAGGTATTGTTACTTCAACCGAGATAGCTGAAAGAGCTAGAGAT
ATTGTACGCGCTACTAATTTGCCTATTCTCGTTGATATAGATACCGGTTTCGGTGGAGTTTTGAATGTGGTCAGAACAGC
ACGTGAAATGGTAGAGGCACATGTGGCGGCAGTACAATTAGAAGATCAGAAGTTGCCTAAAAAGTGCGGCCATTTAAATG
GAAAACAATTAGTAAGCACAGAAGAAATGGTTCAAAAAATTACTGCGATTAAAGAGGTAGCCCCAACACTTGTGATAGTT
GCTAGAACCGACGCACGTGCAGTTGAAGGTTTAGATGATGCTATTTCAAGAGCTCAAGCCTACGTAGAAGCTGGAGCGGA
TATTATATTTCCAGAGGCATTACAAAATGAAGAAGAATTTCGACTTGCTTCAGAAAAAATTGATGCACCACTACTTGCTA
ATATGACTGAATTTGGGAAAACTCCTTATCTAACTGCAGAGGAATTTGAACAAATGGGCTTTGATATTGTTATTTATCCA
GTAACATCTTTACGAGTTGCAGCGAAGGCCTATGAAAAAATCTTTTCATTAATTCGTAAAGAAGGTAGTCAAAAAAATGG
TTTATCTGAAATGCAAACAAGGAAAGAATTATATCAGACAATTTCCTATGATGAATTTGAAGATTTAGATGAAGGTATTG
CGAAAACAGTTCTAGAAGATAAATAA

Upstream 100 bases:

>100_bases
AGTGAACGTATACAGAAGGTTACGAATAATTTTGATGAGGTACAACAAATGAATACAACGGATTTTGTTGATTTATTTGT
AAATTAAGGAGGACTTATAA

Downstream 100 bases:

>100_bases
TAGTATTTGTTTTTCATATGTACTCTTAGGTTTGATCCCTAGGGGTACATATTTCTTTTTGTTAGCTGTTATATAAATTA
CTTACTTTTTTTAGACTTAT

Product: carboxyvinyl-carboxyphosphonate phosphorylmutase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MAWIVDQPISQQELASQFRSQVSEQGIAQIPGAHDAMAGLIAKEAGFQSLYLSGAAYTASRGLPDLGIVTSTEIAERARD
IVRATNLPILVDIDTGFGGVLNVVRTAREMVEAHVAAVQLEDQKLPKKCGHLNGKQLVSTEEMVQKITAIKEVAPTLVIV
ARTDARAVEGLDDAISRAQAYVEAGADIIFPEALQNEEEFRLASEKIDAPLLANMTEFGKTPYLTAEEFEQMGFDIVIYP
VTSLRVAAKAYEKIFSLIRKEGSQKNGLSEMQTRKELYQTISYDEFEDLDEGIAKTVLEDK

Sequences:

>Translated_301_residues
MAWIVDQPISQQELASQFRSQVSEQGIAQIPGAHDAMAGLIAKEAGFQSLYLSGAAYTASRGLPDLGIVTSTEIAERARD
IVRATNLPILVDIDTGFGGVLNVVRTAREMVEAHVAAVQLEDQKLPKKCGHLNGKQLVSTEEMVQKITAIKEVAPTLVIV
ARTDARAVEGLDDAISRAQAYVEAGADIIFPEALQNEEEFRLASEKIDAPLLANMTEFGKTPYLTAEEFEQMGFDIVIYP
VTSLRVAAKAYEKIFSLIRKEGSQKNGLSEMQTRKELYQTISYDEFEDLDEGIAKTVLEDK
>Mature_300_residues
AWIVDQPISQQELASQFRSQVSEQGIAQIPGAHDAMAGLIAKEAGFQSLYLSGAAYTASRGLPDLGIVTSTEIAERARDI
VRATNLPILVDIDTGFGGVLNVVRTAREMVEAHVAAVQLEDQKLPKKCGHLNGKQLVSTEEMVQKITAIKEVAPTLVIVA
RTDARAVEGLDDAISRAQAYVEAGADIIFPEALQNEEEFRLASEKIDAPLLANMTEFGKTPYLTAEEFEQMGFDIVIYPV
TSLRVAAKAYEKIFSLIRKEGSQKNGLSEMQTRKELYQTISYDEFEDLDEGIAKTVLEDK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=284, Percent_Identity=44.0140845070423, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1790445, Length=268, Percent_Identity=29.1044776119403, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71982926, Length=178, Percent_Identity=33.7078651685393, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17561814, Length=178, Percent_Identity=33.7078651685393, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320908, Length=104, Percent_Identity=32.6923076923077, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32996; Mature: 32865

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWIVDQPISQQELASQFRSQVSEQGIAQIPGAHDAMAGLIAKEAGFQSLYLSGAAYTAS
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHH
RGLPDLGIVTSTEIAERARDIVRATNLPILVDIDTGFGGVLNVVRTAREMVEAHVAAVQL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHC
EDQKLPKKCGHLNGKQLVSTEEMVQKITAIKEVAPTLVIVARTDARAVEGLDDAISRAQA
CHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHH
YVEAGADIIFPEALQNEEEFRLASEKIDAPLLANMTEFGKTPYLTAEEFEQMGFDIVIYP
HHHCCCCEECHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEC
VTSLRVAAKAYEKIFSLIRKEGSQKNGLSEMQTRKELYQTISYDEFEDLDEGIAKTVLED
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
K
C
>Mature Secondary Structure 
AWIVDQPISQQELASQFRSQVSEQGIAQIPGAHDAMAGLIAKEAGFQSLYLSGAAYTAS
CCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHH
RGLPDLGIVTSTEIAERARDIVRATNLPILVDIDTGFGGVLNVVRTAREMVEAHVAAVQL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHC
EDQKLPKKCGHLNGKQLVSTEEMVQKITAIKEVAPTLVIVARTDARAVEGLDDAISRAQA
CHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHH
YVEAGADIIFPEALQNEEEFRLASEKIDAPLLANMTEFGKTPYLTAEEFEQMGFDIVIYP
HHHCCCCEECHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEC
VTSLRVAAKAYEKIFSLIRKEGSQKNGLSEMQTRKELYQTISYDEFEDLDEGIAKTVLED
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]