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The map label for this gene is mmgD [H]

Identifier: 23099724

GI number: 23099724

Start: 2312126

End: 2313232

Strand: Reverse

Name: mmgD [H]

Synonym: OB2269

Alternate gene names: 23099724

Gene position: 2313232-2312126 (Counterclockwise)

Preceding gene: 23099726

Following gene: 23099723

Centisome position: 63.72

GC content: 34.42

Gene sequence:

>1107_bases
TTGAAGACAGATGAAAAATTTGTTCCAGGCTTAGATGGTGTGATTGCATCGGAAACAGCAATTTCTTTTTTAGATGTTGA
AAAGGAAAAAATTATCATTAATGGACATGATCTTATCGAATTATCATTAAAACAGGATTATTTAGATATTCTTTATTTAC
TTTTGGAAGGGGATCTGCCAAAAAAAGTAGAGATAACAAAAACAGAGAAATTATTGAAAGATCATTATGAAGTTCCTCAA
GCAGTATTGGATATCTTAAAACTATTACCTAAGGAAACACATCCAATGGATGGAATGCGGACGGCTTTATCTGTACTTGC
AGGATACGATAAAAATATCGAAGATCGCTCAGCTAGTGCGAACAAAGATCGTGCATATCAGTTGCTTGGCCAATTGCCGA
CAATTACAGTAAATAGCTATCGAATTTTGAATAATCAAGAAGTGGTGCAACCAGCCACAGAACTATCTTATAGTGCCAAC
TTTTTATACATGATTACAGGAAAAGAACCATCAGATTTACAAGTGGAAATTTTTGATCGATTATTGTTGCTTTATAGCGA
ACATGAAATGCCAAACTCTACGTTTACTGCTAGAGTAATATCTTCGACCAATTCTGATATGTATGGTGCATTAACTGGAG
CTATATCTTCGTTAAAGGGGAATTTACATGGTGGTGCAAATGAAGCAGTTATGTACATGCTAAATGAAGTAGAAACAGCC
GACGAATTTGAACAATTACTATATAAAAAGTTAGAAAATAAAGAGAAAATTATGGGGTTTGGACATCGTGTCTATATGAA
AAAAATGGACCCACGCGCCCTTATTACAAAAGAAGCACTAAAAAAACTTTGTGAGAAAGATAATGACTATACTTTATTAG
AGATGTGTGAAGCCGGAGAGGAAGTAATGAAAAAAGAAAAAGGGTTGTATCCTAATTTAGATTATTATGCAGCACCTGTT
TATTGGAAATTAGGTATACCGATTTCGCTGTATACACCAATCTTTTTTAGTGCACGTACCGCTGGACTTGCAGCCCATAT
CGTTGAGCAACATTCAAACAATCGTATATTCCGTCCAAGAGTGAAATATATCGGAAAAGATTATTAA

Upstream 100 bases:

>100_bases
TTCTAATTTTCTAATCAATATTATAATATAGGTAGAATAGTTGAAGGATTCTTTCTATTATTTGACTGCGCTTTCAATAT
AAATTTGAGGAGGAATGAAA

Downstream 100 bases:

>100_bases
GGGATAAGAGAGGAGTTTTATTATATGACACAAGTAAAAACGATAAATCAAACAGATAAAATTATTGAAGAAATAGCTGA
TTATGTAGTTCATACAGAAA

Product: citrate synthase 3

Products: NA

Alternate protein names: Citrate synthase 3; Citrate synthase III; Methylcitrate synthase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTDEKFVPGLDGVIASETAISFLDVEKEKIIINGHDLIELSLKQDYLDILYLLLEGDLPKKVEITKTEKLLKDHYEVPQ
AVLDILKLLPKETHPMDGMRTALSVLAGYDKNIEDRSASANKDRAYQLLGQLPTITVNSYRILNNQEVVQPATELSYSAN
FLYMITGKEPSDLQVEIFDRLLLLYSEHEMPNSTFTARVISSTNSDMYGALTGAISSLKGNLHGGANEAVMYMLNEVETA
DEFEQLLYKKLENKEKIMGFGHRVYMKKMDPRALITKEALKKLCEKDNDYTLLEMCEAGEEVMKKEKGLYPNLDYYAAPV
YWKLGIPISLYTPIFFSARTAGLAAHIVEQHSNNRIFRPRVKYIGKDY

Sequences:

>Translated_368_residues
MKTDEKFVPGLDGVIASETAISFLDVEKEKIIINGHDLIELSLKQDYLDILYLLLEGDLPKKVEITKTEKLLKDHYEVPQ
AVLDILKLLPKETHPMDGMRTALSVLAGYDKNIEDRSASANKDRAYQLLGQLPTITVNSYRILNNQEVVQPATELSYSAN
FLYMITGKEPSDLQVEIFDRLLLLYSEHEMPNSTFTARVISSTNSDMYGALTGAISSLKGNLHGGANEAVMYMLNEVETA
DEFEQLLYKKLENKEKIMGFGHRVYMKKMDPRALITKEALKKLCEKDNDYTLLEMCEAGEEVMKKEKGLYPNLDYYAAPV
YWKLGIPISLYTPIFFSARTAGLAAHIVEQHSNNRIFRPRVKYIGKDY
>Mature_368_residues
MKTDEKFVPGLDGVIASETAISFLDVEKEKIIINGHDLIELSLKQDYLDILYLLLEGDLPKKVEITKTEKLLKDHYEVPQ
AVLDILKLLPKETHPMDGMRTALSVLAGYDKNIEDRSASANKDRAYQLLGQLPTITVNSYRILNNQEVVQPATELSYSAN
FLYMITGKEPSDLQVEIFDRLLLLYSEHEMPNSTFTARVISSTNSDMYGALTGAISSLKGNLHGGANEAVMYMLNEVETA
DEFEQLLYKKLENKEKIMGFGHRVYMKKMDPRALITKEALKKLCEKDNDYTLLEMCEAGEEVMKKEKGLYPNLDYYAAPV
YWKLGIPISLYTPIFFSARTAGLAAHIVEQHSNNRIFRPRVKYIGKDY

Specific function: Catalyzes the synthesis of citrate from acetyl-CoA and oxaloacetate. The genomic context suggests that this protein probably functions as a 2-methylcitrate synthase, i.e. catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=323, Percent_Identity=24.7678018575851, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786527, Length=358, Percent_Identity=37.1508379888268, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1786939, Length=377, Percent_Identity=32.0954907161804, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17555174, Length=329, Percent_Identity=26.4437689969605, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319850, Length=337, Percent_Identity=26.7062314540059, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324328, Length=338, Percent_Identity=27.2189349112426, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6325257, Length=341, Percent_Identity=26.6862170087977, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24640126, Length=379, Percent_Identity=26.3852242744063, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24640124, Length=379, Percent_Identity=26.3852242744063, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356863, Length=335, Percent_Identity=23.5820895522388, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5; =2.3.3.1 [H]

Molecular weight: Translated: 41800; Mature: 41800

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDEKFVPGLDGVIASETAISFLDVEKEKIIINGHDLIELSLKQDYLDILYLLLEGDLP
CCCCCCCCCCCCCHHHHHHHHHHEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHCCCCC
KKVEITKTEKLLKDHYEVPQAVLDILKLLPKETHPMDGMRTALSVLAGYDKNIEDRSASA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
NKDRAYQLLGQLPTITVNSYRILNNQEVVQPATELSYSANFLYMITGKEPSDLQVEIFDR
CHHHHHHHHHCCCEEEECCEEEECCCHHHCHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
LLLLYSEHEMPNSTFTARVISSTNSDMYGALTGAISSLKGNLHGGANEAVMYMLNEVETA
HHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCH
DEFEQLLYKKLENKEKIMGFGHRVYMKKMDPRALITKEALKKLCEKDNDYTLLEMCEAGE
HHHHHHHHHHHCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EVMKKEKGLYPNLDYYAAPVYWKLGIPISLYTPIFFSARTAGLAAHIVEQHSNNRIFRPR
HHHHHHCCCCCCCCCEECCEEEEECCCEEECCHHHHHHHHHHHHHHHHHHCCCCCEECCH
VKYIGKDY
HHHCCCCC
>Mature Secondary Structure
MKTDEKFVPGLDGVIASETAISFLDVEKEKIIINGHDLIELSLKQDYLDILYLLLEGDLP
CCCCCCCCCCCCCHHHHHHHHHHEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHCCCCC
KKVEITKTEKLLKDHYEVPQAVLDILKLLPKETHPMDGMRTALSVLAGYDKNIEDRSASA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
NKDRAYQLLGQLPTITVNSYRILNNQEVVQPATELSYSANFLYMITGKEPSDLQVEIFDR
CHHHHHHHHHCCCEEEECCEEEECCCHHHCHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
LLLLYSEHEMPNSTFTARVISSTNSDMYGALTGAISSLKGNLHGGANEAVMYMLNEVETA
HHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCH
DEFEQLLYKKLENKEKIMGFGHRVYMKKMDPRALITKEALKKLCEKDNDYTLLEMCEAGE
HHHHHHHHHHHCCHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EVMKKEKGLYPNLDYYAAPVYWKLGIPISLYTPIFFSARTAGLAAHIVEQHSNNRIFRPR
HHHHHHCCCCCCCCCEECCEEEEECCCEEECCHHHHHHHHHHHHHHHHHHCCCCCEECCH
VKYIGKDY
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759838; 8969508; 9384377 [H]