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The map label for this gene is pnp [H]

Identifier: 23099300

GI number: 23099300

Start: 1877271

End: 1878086

Strand: Reverse

Name: pnp [H]

Synonym: OB1845

Alternate gene names: 23099300

Gene position: 1878086-1877271 (Counterclockwise)

Preceding gene: 23099301

Following gene: 23099299

Centisome position: 51.73

GC content: 35.05

Gene sequence:

>816_bases
ATGGATAATCAATTTATTCAAGAAGCAGCAAGTTTTATAAAAGAAAAAATGAACAGAGAACCATCAATAGGTTTAATCTT
AGGATCTGGTCTAGGTGTATTGGCAGATGAAATTGAGGATGCAATCCATATTGAATATAAAGATATCCCTCATTTTCCTA
CGTCAACGGTTTCTGGTCATAAAGGTCAACTAGTAATTGGTGCAATGGAAGGAAAGCAAGTTATTGCAATGCAAGGTCGT
TTTCATTACTACGAAGGGTATACCATGCAACAAGTAACATTTCCAGTACGAGTAATGAAAGAATTAAATATAGAACAATT
AATTGTTACAAATGCAGCTGGAGGAGTAAATGAATCTTTCTCTGCTGGTGACTTAATGTTAATTACAGATCATATTAATC
AAATGGGAGACAATCCATTAATAGGTCCAAATGATAATAGACATGGGCCTCGTTTTCCTGATATGTCTACTGCGTACGAT
AAAGACTTATTAAAACTAGCAAAAAATATTGCTGGAAAATTAGATTTAGCTGTACAACAAGGTGTGTACGTTGGTAATAG
TGGACCCGTTTATGAAACACCGGCTGAGGTTAGGATGATACGTATTCTAGGTGGAGATGCAGTAGGAATGTCAACTGTTC
CTGAGGTAACTGTAGCTAACCATGCAGGTATTAAGGTACTTGGAATTTCATGTATTTCAAATATGGCAGCAGGTATATTA
GATCAACCTTTAACACATGATGAAGTGATCGAAACAACAGATAAAGTGAGAGAGAGCTTTTTACAATTTGTTCGAAATAT
CATTCAATCAATGTAA

Upstream 100 bases:

>100_bases
CGGATATAGGAGCAACTATTGCAGATAACTTTGGAATTAAGCTGCCTGAACACGGAGAAAGCTTTTTGAACGATATTAAG
TAGTAATGGGGGAAGATAGA

Downstream 100 bases:

>100_bases
TGGTACAAGTTTGGAGAGGGTGGAGAAATTATGAATACGGTAGAAATTATTAATAAGAAAAAATATGGAGAAGTGTTAAC
TTACGAAGAATTACAGCATG

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MDNQFIQEAASFIKEKMNREPSIGLILGSGLGVLADEIEDAIHIEYKDIPHFPTSTVSGHKGQLVIGAMEGKQVIAMQGR
FHYYEGYTMQQVTFPVRVMKELNIEQLIVTNAAGGVNESFSAGDLMLITDHINQMGDNPLIGPNDNRHGPRFPDMSTAYD
KDLLKLAKNIAGKLDLAVQQGVYVGNSGPVYETPAEVRMIRILGGDAVGMSTVPEVTVANHAGIKVLGISCISNMAAGIL
DQPLTHDEVIETTDKVRESFLQFVRNIIQSM

Sequences:

>Translated_271_residues
MDNQFIQEAASFIKEKMNREPSIGLILGSGLGVLADEIEDAIHIEYKDIPHFPTSTVSGHKGQLVIGAMEGKQVIAMQGR
FHYYEGYTMQQVTFPVRVMKELNIEQLIVTNAAGGVNESFSAGDLMLITDHINQMGDNPLIGPNDNRHGPRFPDMSTAYD
KDLLKLAKNIAGKLDLAVQQGVYVGNSGPVYETPAEVRMIRILGGDAVGMSTVPEVTVANHAGIKVLGISCISNMAAGIL
DQPLTHDEVIETTDKVRESFLQFVRNIIQSM
>Mature_271_residues
MDNQFIQEAASFIKEKMNREPSIGLILGSGLGVLADEIEDAIHIEYKDIPHFPTSTVSGHKGQLVIGAMEGKQVIAMQGR
FHYYEGYTMQQVTFPVRVMKELNIEQLIVTNAAGGVNESFSAGDLMLITDHINQMGDNPLIGPNDNRHGPRFPDMSTAYD
KDLLKLAKNIAGKLDLAVQQGVYVGNSGPVYETPAEVRMIRILGGDAVGMSTVPEVTVANHAGIKVLGISCISNMAAGIL
DQPLTHDEVIETTDKVRESFLQFVRNIIQSM

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=284, Percent_Identity=44.7183098591549, Blast_Score=240, Evalue=9e-64,
Organism=Escherichia coli, GI1788746, Length=256, Percent_Identity=42.1875, Blast_Score=227, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI17541190, Length=272, Percent_Identity=44.4852941176471, Blast_Score=224, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6323238, Length=280, Percent_Identity=45, Blast_Score=206, Evalue=3e-54,
Organism=Drosophila melanogaster, GI45552887, Length=277, Percent_Identity=46.2093862815885, Blast_Score=250, Evalue=7e-67,
Organism=Drosophila melanogaster, GI45552885, Length=277, Percent_Identity=46.2093862815885, Blast_Score=250, Evalue=9e-67,
Organism=Drosophila melanogaster, GI24656090, Length=277, Percent_Identity=46.2093862815885, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24656093, Length=277, Percent_Identity=46.2093862815885, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24762376, Length=258, Percent_Identity=36.8217054263566, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29568; Mature: 29568

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNQFIQEAASFIKEKMNREPSIGLILGSGLGVLADEIEDAIHIEYKDIPHFPTSTVSGH
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCC
KGQLVIGAMEGKQVIAMQGRFHYYEGYTMQQVTFPVRVMKELNIEQLIVTNAAGGVNESF
CCCEEEEECCCCEEEEEECCEEEECCEEEEHHHHHHHHHHHCCHHHEEEECCCCCCCCCC
SAGDLMLITDHINQMGDNPLIGPNDNRHGPRFPDMSTAYDKDLLKLAKNIAGKLDLAVQQ
CCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEHHC
GVYVGNSGPVYETPAEVRMIRILGGDAVGMSTVPEVTVANHAGIKVLGISCISNMAAGIL
CEEECCCCCCEECHHHEEEEEEECCCCCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHH
DQPLTHDEVIETTDKVRESFLQFVRNIIQSM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDNQFIQEAASFIKEKMNREPSIGLILGSGLGVLADEIEDAIHIEYKDIPHFPTSTVSGH
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCC
KGQLVIGAMEGKQVIAMQGRFHYYEGYTMQQVTFPVRVMKELNIEQLIVTNAAGGVNESF
CCCEEEEECCCCEEEEEECCEEEECCEEEEHHHHHHHHHHHCCHHHEEEECCCCCCCCCC
SAGDLMLITDHINQMGDNPLIGPNDNRHGPRFPDMSTAYDKDLLKLAKNIAGKLDLAVQQ
CCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEHHC
GVYVGNSGPVYETPAEVRMIRILGGDAVGMSTVPEVTVANHAGIKVLGISCISNMAAGIL
CEEECCCCCCEECHHHEEEEEEECCCCCCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHH
DQPLTHDEVIETTDKVRESFLQFVRNIIQSM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]