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The map label for this gene is gudB [H]

Identifier: 23099265

GI number: 23099265

Start: 1846686

End: 1847966

Strand: Reverse

Name: gudB [H]

Synonym: OB1810

Alternate gene names: 23099265

Gene position: 1847966-1846686 (Counterclockwise)

Preceding gene: 23099266

Following gene: 23099264

Centisome position: 50.9

GC content: 37.24

Gene sequence:

>1281_bases
ATGGTAGCCGATAAAGCAGCAGATTCTTCAAATGTGAATCAAGAAAATATGGATGTTTTAAATACAACACAAACTATTAT
TAAAAGTGCATTGGATAAACTTGGTTACCCAGAAGAGGTATTTGAATTATTAAAAGAACCGATGCGGATTCTAACGGTCC
GTATACCTGTAAGAATGGATGACGGTAATGTAAAAGTGTTTACAGGTTATCGTGCTCAGCACAATGATGCAGTGGGTCCT
ACCAAAGGTGGAATACGCTTTCATCCTAATGTTACTGAAACAGAAGTAAAGGCGCTTTCTATTTGGATGAGTTTGAAATC
GGGGATTGTTGATCTTCCTTATGGTGGAGCTAAAGGAGGAATTATTTGCGATCCTCGTGAAATGTCTTTCCGTGAATTAG
AAGCATTAAGTCGCGGTTATGTACGTGCGGTTAGCCAAATAGTAGGTCCGACAAAAGATATACCTGCACCTGATGTATTT
ACTAATTCTCAAATTATGGCGTGGATGATGGATGAATATAGTAAGATTGATGAATTTAACAATCCTGGGTTTATAACTGG
TAAACCAATTGTCCTTGGTGGATCGCATGGGAGAGAATCTGCTACTGCAAAAGGTGTAACAATCGTTTTAAATGAAGCAG
CTAAAAAGAAAGGCATAGATATAAAAGGAGCCCGTGTTGTTATACAAGGGTTTGGAAATGCTGGAAGCTTTTTAGCTAAG
TTTTTACATGATGCCGGGGCGAAAGTTGTCGCAATATCTGATGCGTACGGTGCCTTGTATGATCCAGAAGGCTTGGATAT
TGATTATTTATTGGATCGCCGTGACAGTTTTGGTACGGTAACGAAATTATTTAATAATACAATTTCCAATGATGCATTAT
TTGAATTAGACTGCGATATTATTGTACCAGCTGCTGTTGAGAATCAGATAACTCGTGAAAATGCTCATAATATAAAAGCA
TCTATTGTAGTTGAAGCAGCTAACGGACCTACAACGATGGAAGCAACAAAAATACTAACAGAACGTGACATATTAATTGT
ACCAGATGTTTTAGCTTCTGCTGGAGGAGTTACTGTATCTTACTTTGAATGGGTGCAAAACAATCAAGGGTTCTATTGGT
CAGAAGAAGAAATAGATAATAAATTACACGAAATAATGATTAAATCATTTAACAACATCTATAATATGTCGAAAACACGG
AGAATTGATATGAGATTAGCTGCATATATGGTTGGTGTTAGAAAGATGGCAGAAGCATCTCGTTTCCGTGGTTGGGTATA
G

Upstream 100 bases:

>100_bases
AGGCGTAAGATTTGATTTATGTTATTGCATATTCTGTTTGAAAGTGTATACTAATTTTTGAGTAGACAAAACAAGGATTT
ATATTGTAGGAGGTTAATTC

Downstream 100 bases:

>100_bases
GTCTGTTGTTTAAAATTGATAAATAGGTAACTTAGAGTGGCGCTGGACATAGCAAATAGGGATAATTCAAAAATTGAACA
ATTCAAGTACGTAAAGCTTT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NAD-GDH [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MVADKAADSSNVNQENMDVLNTTQTIIKSALDKLGYPEEVFELLKEPMRILTVRIPVRMDDGNVKVFTGYRAQHNDAVGP
TKGGIRFHPNVTETEVKALSIWMSLKSGIVDLPYGGAKGGIICDPREMSFRELEALSRGYVRAVSQIVGPTKDIPAPDVF
TNSQIMAWMMDEYSKIDEFNNPGFITGKPIVLGGSHGRESATAKGVTIVLNEAAKKKGIDIKGARVVIQGFGNAGSFLAK
FLHDAGAKVVAISDAYGALYDPEGLDIDYLLDRRDSFGTVTKLFNNTISNDALFELDCDIIVPAAVENQITRENAHNIKA
SIVVEAANGPTTMEATKILTERDILIVPDVLASAGGVTVSYFEWVQNNQGFYWSEEEIDNKLHEIMIKSFNNIYNMSKTR
RIDMRLAAYMVGVRKMAEASRFRGWV

Sequences:

>Translated_426_residues
MVADKAADSSNVNQENMDVLNTTQTIIKSALDKLGYPEEVFELLKEPMRILTVRIPVRMDDGNVKVFTGYRAQHNDAVGP
TKGGIRFHPNVTETEVKALSIWMSLKSGIVDLPYGGAKGGIICDPREMSFRELEALSRGYVRAVSQIVGPTKDIPAPDVF
TNSQIMAWMMDEYSKIDEFNNPGFITGKPIVLGGSHGRESATAKGVTIVLNEAAKKKGIDIKGARVVIQGFGNAGSFLAK
FLHDAGAKVVAISDAYGALYDPEGLDIDYLLDRRDSFGTVTKLFNNTISNDALFELDCDIIVPAAVENQITRENAHNIKA
SIVVEAANGPTTMEATKILTERDILIVPDVLASAGGVTVSYFEWVQNNQGFYWSEEEIDNKLHEIMIKSFNNIYNMSKTR
RIDMRLAAYMVGVRKMAEASRFRGWV
>Mature_426_residues
MVADKAADSSNVNQENMDVLNTTQTIIKSALDKLGYPEEVFELLKEPMRILTVRIPVRMDDGNVKVFTGYRAQHNDAVGP
TKGGIRFHPNVTETEVKALSIWMSLKSGIVDLPYGGAKGGIICDPREMSFRELEALSRGYVRAVSQIVGPTKDIPAPDVF
TNSQIMAWMMDEYSKIDEFNNPGFITGKPIVLGGSHGRESATAKGVTIVLNEAAKKKGIDIKGARVVIQGFGNAGSFLAK
FLHDAGAKVVAISDAYGALYDPEGLDIDYLLDRRDSFGTVTKLFNNTISNDALFELDCDIIVPAAVENQITRENAHNIKA
SIVVEAANGPTTMEATKILTERDILIVPDVLASAGGVTVSYFEWVQNNQGFYWSEEEIDNKLHEIMIKSFNNIYNMSKTR
RIDMRLAAYMVGVRKMAEASRFRGWV

Specific function: The wild-type version has little or no activity [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=341, Percent_Identity=40.7624633431085, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI4885281, Length=364, Percent_Identity=39.2857142857143, Blast_Score=270, Evalue=3e-72,
Organism=Escherichia coli, GI1788059, Length=369, Percent_Identity=31.7073170731707, Blast_Score=176, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17544676, Length=357, Percent_Identity=42.5770308123249, Blast_Score=265, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6324951, Length=408, Percent_Identity=28.6764705882353, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6319256, Length=395, Percent_Identity=29.1139240506329, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI45553475, Length=341, Percent_Identity=43.9882697947214, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI45549226, Length=340, Percent_Identity=43.8235294117647, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24649283, Length=390, Percent_Identity=36.4102564102564, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24649460, Length=279, Percent_Identity=42.6523297491039, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.2 [H]

Molecular weight: Translated: 46928; Mature: 46928

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVADKAADSSNVNQENMDVLNTTQTIIKSALDKLGYPEEVFELLKEPMRILTVRIPVRMD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEEEEEEC
DGNVKVFTGYRAQHNDAVGPTKGGIRFHPNVTETEVKALSIWMSLKSGIVDLPYGGAKGG
CCCEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCC
IICDPREMSFRELEALSRGYVRAVSQIVGPTKDIPAPDVFTNSQIMAWMMDEYSKIDEFN
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
NPGFITGKPIVLGGSHGRESATAKGVTIVLNEAAKKKGIDIKGARVVIQGFGNAGSFLAK
CCCEECCCCEEEECCCCCCCCCCCCEEEEEECHHHHCCCCCCCCEEEEEECCCHHHHHHH
FLHDAGAKVVAISDAYGALYDPEGLDIDYLLDRRDSFGTVTKLFNNTISNDALFELDCDI
HHHHCCCEEEEEECCCCCCCCCCCCCCHHEECCCCCHHHHHHHHHCCCCCCEEEEECCCE
IVPAAVENQITRENAHNIKASIVVEAANGPTTMEATKILTERDILIVPDVLASAGGVTVS
EECHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHCCCEEEECHHHHCCCCCEEE
YFEWVQNNQGFYWSEEEIDNKLHEIMIKSFNNIYNMSKTRRIDMRLAAYMVGVRKMAEAS
EEHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RFRGWV
HHCCCC
>Mature Secondary Structure
MVADKAADSSNVNQENMDVLNTTQTIIKSALDKLGYPEEVFELLKEPMRILTVRIPVRMD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEEEEEEC
DGNVKVFTGYRAQHNDAVGPTKGGIRFHPNVTETEVKALSIWMSLKSGIVDLPYGGAKGG
CCCEEEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCC
IICDPREMSFRELEALSRGYVRAVSQIVGPTKDIPAPDVFTNSQIMAWMMDEYSKIDEFN
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
NPGFITGKPIVLGGSHGRESATAKGVTIVLNEAAKKKGIDIKGARVVIQGFGNAGSFLAK
CCCEECCCCEEEECCCCCCCCCCCCEEEEEECHHHHCCCCCCCCEEEEEECCCHHHHHHH
FLHDAGAKVVAISDAYGALYDPEGLDIDYLLDRRDSFGTVTKLFNNTISNDALFELDCDI
HHHHCCCEEEEEECCCCCCCCCCCCCCHHEECCCCCHHHHHHHHHCCCCCCEEEEECCCE
IVPAAVENQITRENAHNIKASIVVEAANGPTTMEATKILTERDILIVPDVLASAGGVTVS
EECHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHCCCEEEECHHHHCCCCCEEE
YFEWVQNNQGFYWSEEEIDNKLHEIMIKSFNNIYNMSKTRRIDMRLAAYMVGVRKMAEAS
EEHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
RFRGWV
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829940 [H]