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The map label for this gene is ansA [H]

Identifier: 23099263

GI number: 23099263

Start: 1844270

End: 1845241

Strand: Reverse

Name: ansA [H]

Synonym: OB1808

Alternate gene names: 23099263

Gene position: 1845241-1844270 (Counterclockwise)

Preceding gene: 23099264

Following gene: 23099262

Centisome position: 50.83

GC content: 33.23

Gene sequence:

>972_bases
ATGACTAAAAAAATATTATTATTAGCTACTGGTGGTACCATAGCAAGTGAGAAGAAACCAAACAGTGATAGATTAGAATC
TGGAGTCTTGACTGGAGAAGAATTACTTAAACAATGTGATATTCCTAGTGGAATTGAAGTAGAGGTTCATTCTATATTTC
AAAAAGCAAGCATCCACTTAACATTTCAAGATTTACTGGTATTAAAATCGAAAATTGATGAACTTTTACTTAATAGTAGT
TTTGATGGAATAGTCATTACTCAAGGTACCGACACATTAGAAGAATCTTCATACTTTATGAATCTTATTACTGATTACCA
TATTCCCATTGTTTTTACAGGTTCTCAGCGTTCTCCTTTAGAAGTTGGAACGGATTCTTTTATTAATATAAAAAATGCAG
TTCTAACGGCTTGTAGTGAAGAACTAAATGAAGTGGGTGTGACAGTTGTATTTAATGAGCGTATTTTTCATTCACGTTAT
GTTCGAAAAGAACACGCTTCAAATATTCAGGGATTCAATGCGTTTGGATATGGATATTTAGGGATTATCGATAATAATCA
AGTGCGTGTTTTCCAAAAGCCTACGTATAAGGATTATTATAAATTACAAAAGAAATTGCCTGAAGTAGATATTATTAAAG
TGTATATGAATACTGATGGTAAGTATATTAAAGCAGCGAGAGATAATCAGGTTGATGGAATTGTAATTGAAGGGGTTGGA
AGAGGACAAGTATCCCCAACGATGATGCAAGAAATTAAACAATGTATCGAAGAGGGTATTGCAGTGGTTATTACAACATC
AGCTGTAGAGGGAGAAGTGTATCCAACTTACGATTATCAAGGAAGTACATATGATTTATTACAGAACGGTGTAATTCTTG
GAAAAGATTATGATAGTAAAAAAGCGAGAATCAAGCTCATGGTAGGGCTTGCTAGTGAAATACCTCTACAGACACTTTTT
CATCCATTCTAA

Upstream 100 bases:

>100_bases
TAAATAGAGATTGTATATTTTAAGTAAGATATACTTGAAAGATTTTCTTTGGAAAAGGATAATATAGGAAGATATAATTA
CATAAAAAGAAGGGTTTGCT

Downstream 100 bases:

>100_bases
TTTAGATGCTTTTTTGGCTAAGTGAATTAATTTCAAAATAAAATTAATGTTCAAATTCTGGGTTTGAATTAAATGACAGA
TGGGGACAAATTCCACAGGA

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTKKILLLATGGTIASEKKPNSDRLESGVLTGEELLKQCDIPSGIEVEVHSIFQKASIHLTFQDLLVLKSKIDELLLNSS
FDGIVITQGTDTLEESSYFMNLITDYHIPIVFTGSQRSPLEVGTDSFINIKNAVLTACSEELNEVGVTVVFNERIFHSRY
VRKEHASNIQGFNAFGYGYLGIIDNNQVRVFQKPTYKDYYKLQKKLPEVDIIKVYMNTDGKYIKAARDNQVDGIVIEGVG
RGQVSPTMMQEIKQCIEEGIAVVITTSAVEGEVYPTYDYQGSTYDLLQNGVILGKDYDSKKARIKLMVGLASEIPLQTLF
HPF

Sequences:

>Translated_323_residues
MTKKILLLATGGTIASEKKPNSDRLESGVLTGEELLKQCDIPSGIEVEVHSIFQKASIHLTFQDLLVLKSKIDELLLNSS
FDGIVITQGTDTLEESSYFMNLITDYHIPIVFTGSQRSPLEVGTDSFINIKNAVLTACSEELNEVGVTVVFNERIFHSRY
VRKEHASNIQGFNAFGYGYLGIIDNNQVRVFQKPTYKDYYKLQKKLPEVDIIKVYMNTDGKYIKAARDNQVDGIVIEGVG
RGQVSPTMMQEIKQCIEEGIAVVITTSAVEGEVYPTYDYQGSTYDLLQNGVILGKDYDSKKARIKLMVGLASEIPLQTLF
HPF
>Mature_322_residues
TKKILLLATGGTIASEKKPNSDRLESGVLTGEELLKQCDIPSGIEVEVHSIFQKASIHLTFQDLLVLKSKIDELLLNSSF
DGIVITQGTDTLEESSYFMNLITDYHIPIVFTGSQRSPLEVGTDSFINIKNAVLTACSEELNEVGVTVVFNERIFHSRYV
RKEHASNIQGFNAFGYGYLGIIDNNQVRVFQKPTYKDYYKLQKKLPEVDIIKVYMNTDGKYIKAARDNQVDGIVIEGVGR
GQVSPTMMQEIKQCIEEGIAVVITTSAVEGEVYPTYDYQGSTYDLLQNGVILGKDYDSKKARIKLMVGLASEIPLQTLFH
PF

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=236, Percent_Identity=31.3559322033898, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788065, Length=331, Percent_Identity=24.773413897281, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=259, Percent_Identity=25.4826254826255, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193207374, Length=259, Percent_Identity=25.4826254826255, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558280, Length=259, Percent_Identity=25.4826254826255, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17558278, Length=259, Percent_Identity=25.4826254826255, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323189, Length=286, Percent_Identity=29.020979020979, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323187, Length=286, Percent_Identity=29.020979020979, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323186, Length=286, Percent_Identity=29.020979020979, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323184, Length=286, Percent_Identity=29.020979020979, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320527, Length=334, Percent_Identity=30.2395209580838, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645530, Length=236, Percent_Identity=25.8474576271186, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36055; Mature: 35924

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00144 ASN_GLN_ASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKILLLATGGTIASEKKPNSDRLESGVLTGEELLKQCDIPSGIEVEVHSIFQKASIHL
CCCEEEEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHCCCCCCCEEHHHHHHHHHEEEE
TFQDLLVLKSKIDELLLNSSFDGIVITQGTDTLEESSYFMNLITDYHIPIVFTGSQRSPL
EHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
EVGTDSFINIKNAVLTACSEELNEVGVTVVFNERIFHSRYVRKEHASNIQGFNAFGYGYL
CCCCCCEEEHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEE
GIIDNNQVRVFQKPTYKDYYKLQKKLPEVDIIKVYMNTDGKYIKAARDNQVDGIVIEGVG
EEEECCEEEEEECCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEECCCCCCCEEEEEECC
RGQVSPTMMQEIKQCIEEGIAVVITTSAVEGEVYPTYDYQGSTYDLLQNGVILGKDYDSK
CCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCCHHHHHHCCEEEECCCCCC
KARIKLMVGLASEIPLQTLFHPF
CEEEEEEEECCCCCCHHHHCCCC
>Mature Secondary Structure 
TKKILLLATGGTIASEKKPNSDRLESGVLTGEELLKQCDIPSGIEVEVHSIFQKASIHL
CCEEEEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHCCCCCCCEEHHHHHHHHHEEEE
TFQDLLVLKSKIDELLLNSSFDGIVITQGTDTLEESSYFMNLITDYHIPIVFTGSQRSPL
EHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
EVGTDSFINIKNAVLTACSEELNEVGVTVVFNERIFHSRYVRKEHASNIQGFNAFGYGYL
CCCCCCEEEHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEE
GIIDNNQVRVFQKPTYKDYYKLQKKLPEVDIIKVYMNTDGKYIKAARDNQVDGIVIEGVG
EEEECCEEEEEECCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEECCCCCCCEEEEEECC
RGQVSPTMMQEIKQCIEEGIAVVITTSAVEGEVYPTYDYQGSTYDLLQNGVILGKDYDSK
CCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCCHHHHHHCCEEEECCCCCC
KARIKLMVGLASEIPLQTLFHPF
CEEEEEEEECCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]