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The map label for this gene is grlA [H]

Identifier: 23099149

GI number: 23099149

Start: 1734217

End: 1736667

Strand: Direct

Name: grlA [H]

Synonym: OB1694

Alternate gene names: 23099149

Gene position: 1734217-1736667 (Clockwise)

Preceding gene: 23099148

Following gene: 23099150

Centisome position: 47.77

GC content: 37.21

Gene sequence:

>2451_bases
TTGTCACAAACAGAAAAATATCTCGATCTACCTCTAGAAGAAGTAATCGGAGATCGATTTGGTAGATATAGTAAATACAT
TATTCAAGACCGTGCCCTTCCCGATGCAAGGGATGGCTTGAAACCTGTACAACGACGTATTTTATATGCAATGCACATGG
AGAGAAATACTCATGATAAGCACTTTCGTAAATCAGCTAAGACTGTAGGTACCGTAATTGGTAATTATCATCCGCATGGT
GATTCTTCCGTTTACGATGCGATGGTTCGATTAAGTCAAGACTGGAAAGTAAGAAATGGACTTATTGAAATGCACGGAAA
TAATGGGAGTATAGATGGTGACCCACCTGCAGCTATGCGTTACACTGAAGCACGTTTATCCAGTATTTCTGCAGAGCTTT
TACGAGATATTGACAAACAAACAGTTGATTATATACCTAACTTTGATGAAACAACAGATGAACCAGTTGTATTACCCGCA
AAATTCCCAAATTTACTTGTGAATGGATCTACGGGTATTTCAGCAGGTTATGCTACGGACATCCCACCTCATAATCTTGA
GGAAGTGATGGACGCGGTAATTTTGAAAATTGATAAACCAGAAGCTTCGATAGATGATTTAATGAAGTTGATGAAAGGAC
CAGACTTTCCTACTGGTGGTATAATTCAAGGAGTAGAAGGTATTCGCAAAGCATATGAAACAGGAAGAGGAAAAATTATT
GTTCGAGGAAAAGCCGTGGTTGAAACTATGAAAGGCGGCCGAGAGCAAATTGTTGTCACCGAAGTACCTTTTGAAGTAAA
TAAGGCAACAATGGTTAAGAAAATGGATGAATTACGTATTGATCGTAAAGTAGAAGGAATTGCTGAAGTACGTGATGAGA
CTGATCGAACTGGGTTAAGAGTGGTAATTGAACTCAAAAAAGATGCCAATAGTGAAGGTGTACTAAACTATTTATATAAA
AATACGGATTTGCAAATCACGTATCACTTTAACATGGTCGCGATTCAAGATAAAACACCAAAACTATTATCTTTACCTTC
GTTACTTGACGCTTATATTGCACATCAAAAAGAAGTTGTAACAAGGCAGATCACCTATGATCTAAACAAAGCAAATGCAC
GCGCGCATATCGTAGAAGGGTTAATTAAAGCCATCTCCATACTTGATGAATTGATCGCTACCATTCGTGCATCAAAAGAT
AAAGGTGATGCGAAAGCACAGATTAAAGCGAAATACGGCTTTACAGAAGAACAAGCAGAAGCAATCGTTATGCTCCAATT
ATATCGCTTGACGAATACAGATATCGTGGAATTAGAAAAAGAAGCAGCTGAATTAAAAGAAAAAATTAAAGAGTACGAGT
CGATTTTAAGCAGTGACAAGAAGCTTTTCCAAACAATCAAAAAAGAGTTACGACAGATTAAAAAGAAATTTGCAGATCCT
CGTAGAACAGTCATCGAAGAGAAAATTGAGGAACTAAAAATAAATATTGAAGTAACTGTCGCTAGTGAGGATGTACTTGT
TTCAGTCACGCGGGACGGTTATCTAAAAAGGACTAGCTTACGTTCTTATGCTGCTTCAAATGAGGAAGAGTTACCAGTAA
AGGACGATGATTACCCTATATCATTACTGGAAGTAAATACAACGGACAATTTATTATTATTTACAAATAAAGGTAAATAT
ATTTTATGTCCTGTTCATGAATTACCAGATATTCGTTGGAAGGATATGGGACAGCATATTTCTAATATCGTTCCGTTGGA
TAAAGAAGAATACCTCATTCAGTGTATGCCAGTTCGTGATTTTAAATCAGCCGAAGGATATCTGATCTTCTTTACGAAAA
ACGGTATGGTTAAACGAAGTGAATTCCAGTTATACGATGCCCAGCGGAGGTCAAAAGCATTAATTGCTCTTAATCTCAAA
GGGGACGATGAAGTTGTTCATGTTAGCCATACAAATGGAAATATGGATGTTTTTATCGCTACGTATAAAGGGTACGGATT
ATGGTACCATGAGTCGGAAATCTCTGTGGTCGGTCAACGAGCTGCTGGAGTTAAATCAGTTTCGTTAAAAGACGATGATG
AAGTAGTAAATGGACAAGTATTCGACGATCTATCAGAACCATCTTTAGCATTAATTACACAACGCGGATCTTGCAAACGG
ATGAAATTGAAAGATTTTGAAAAAACTACCCGTGCAAAACGTGGGGTAATAATGCTTCGTGAGTTAAAAAACAAAGCTCA
TCGTGTAAAAGGCTTTTTCGCTTTACAAGACCATGATGGACAACAAATTTGTTTCCAGACAGCAAATGGAAATACCCATG
AAATTGTACCACTAACATTAAAGGCAACAGATCGATATAACAATGGATCATTTGTTATTGACACGGATCATGAAGGTGAA
GTCATCCATGTTTGGAAGCAAGCAAATTATGAAAAACCGTTCGATGAATAA

Upstream 100 bases:

>100_bases
TATACTTGAGAACGAAAATATTCACACAGAAGAGTAAAGTGACTAATAGATGTTGTTCTTTTAGGTCCTTTTGAACACGC
AATTATAGGGGGGATTCAAT

Downstream 100 bases:

>100_bases
TTAAATAACTACAAATTTTAGGCAAATGTAGGAAATCTACATTTGCCTATTTCATTCCTTTTGATAAAATAGAAAGTATA
ATCGAATGTATGATAATTCA

Product: DNA topoisomerase IV subunit A

Products: NA

Alternate protein names: Topoisomerase IV subunit A [H]

Number of amino acids: Translated: 816; Mature: 815

Protein sequence:

>816_residues
MSQTEKYLDLPLEEVIGDRFGRYSKYIIQDRALPDARDGLKPVQRRILYAMHMERNTHDKHFRKSAKTVGTVIGNYHPHG
DSSVYDAMVRLSQDWKVRNGLIEMHGNNGSIDGDPPAAMRYTEARLSSISAELLRDIDKQTVDYIPNFDETTDEPVVLPA
KFPNLLVNGSTGISAGYATDIPPHNLEEVMDAVILKIDKPEASIDDLMKLMKGPDFPTGGIIQGVEGIRKAYETGRGKII
VRGKAVVETMKGGREQIVVTEVPFEVNKATMVKKMDELRIDRKVEGIAEVRDETDRTGLRVVIELKKDANSEGVLNYLYK
NTDLQITYHFNMVAIQDKTPKLLSLPSLLDAYIAHQKEVVTRQITYDLNKANARAHIVEGLIKAISILDELIATIRASKD
KGDAKAQIKAKYGFTEEQAEAIVMLQLYRLTNTDIVELEKEAAELKEKIKEYESILSSDKKLFQTIKKELRQIKKKFADP
RRTVIEEKIEELKINIEVTVASEDVLVSVTRDGYLKRTSLRSYAASNEEELPVKDDDYPISLLEVNTTDNLLLFTNKGKY
ILCPVHELPDIRWKDMGQHISNIVPLDKEEYLIQCMPVRDFKSAEGYLIFFTKNGMVKRSEFQLYDAQRRSKALIALNLK
GDDEVVHVSHTNGNMDVFIATYKGYGLWYHESEISVVGQRAAGVKSVSLKDDDEVVNGQVFDDLSEPSLALITQRGSCKR
MKLKDFEKTTRAKRGVIMLRELKNKAHRVKGFFALQDHDGQQICFQTANGNTHEIVPLTLKATDRYNNGSFVIDTDHEGE
VIHVWKQANYEKPFDE

Sequences:

>Translated_816_residues
MSQTEKYLDLPLEEVIGDRFGRYSKYIIQDRALPDARDGLKPVQRRILYAMHMERNTHDKHFRKSAKTVGTVIGNYHPHG
DSSVYDAMVRLSQDWKVRNGLIEMHGNNGSIDGDPPAAMRYTEARLSSISAELLRDIDKQTVDYIPNFDETTDEPVVLPA
KFPNLLVNGSTGISAGYATDIPPHNLEEVMDAVILKIDKPEASIDDLMKLMKGPDFPTGGIIQGVEGIRKAYETGRGKII
VRGKAVVETMKGGREQIVVTEVPFEVNKATMVKKMDELRIDRKVEGIAEVRDETDRTGLRVVIELKKDANSEGVLNYLYK
NTDLQITYHFNMVAIQDKTPKLLSLPSLLDAYIAHQKEVVTRQITYDLNKANARAHIVEGLIKAISILDELIATIRASKD
KGDAKAQIKAKYGFTEEQAEAIVMLQLYRLTNTDIVELEKEAAELKEKIKEYESILSSDKKLFQTIKKELRQIKKKFADP
RRTVIEEKIEELKINIEVTVASEDVLVSVTRDGYLKRTSLRSYAASNEEELPVKDDDYPISLLEVNTTDNLLLFTNKGKY
ILCPVHELPDIRWKDMGQHISNIVPLDKEEYLIQCMPVRDFKSAEGYLIFFTKNGMVKRSEFQLYDAQRRSKALIALNLK
GDDEVVHVSHTNGNMDVFIATYKGYGLWYHESEISVVGQRAAGVKSVSLKDDDEVVNGQVFDDLSEPSLALITQRGSCKR
MKLKDFEKTTRAKRGVIMLRELKNKAHRVKGFFALQDHDGQQICFQTANGNTHEIVPLTLKATDRYNNGSFVIDTDHEGE
VIHVWKQANYEKPFDE
>Mature_815_residues
SQTEKYLDLPLEEVIGDRFGRYSKYIIQDRALPDARDGLKPVQRRILYAMHMERNTHDKHFRKSAKTVGTVIGNYHPHGD
SSVYDAMVRLSQDWKVRNGLIEMHGNNGSIDGDPPAAMRYTEARLSSISAELLRDIDKQTVDYIPNFDETTDEPVVLPAK
FPNLLVNGSTGISAGYATDIPPHNLEEVMDAVILKIDKPEASIDDLMKLMKGPDFPTGGIIQGVEGIRKAYETGRGKIIV
RGKAVVETMKGGREQIVVTEVPFEVNKATMVKKMDELRIDRKVEGIAEVRDETDRTGLRVVIELKKDANSEGVLNYLYKN
TDLQITYHFNMVAIQDKTPKLLSLPSLLDAYIAHQKEVVTRQITYDLNKANARAHIVEGLIKAISILDELIATIRASKDK
GDAKAQIKAKYGFTEEQAEAIVMLQLYRLTNTDIVELEKEAAELKEKIKEYESILSSDKKLFQTIKKELRQIKKKFADPR
RTVIEEKIEELKINIEVTVASEDVLVSVTRDGYLKRTSLRSYAASNEEELPVKDDDYPISLLEVNTTDNLLLFTNKGKYI
LCPVHELPDIRWKDMGQHISNIVPLDKEEYLIQCMPVRDFKSAEGYLIFFTKNGMVKRSEFQLYDAQRRSKALIALNLKG
DDEVVHVSHTNGNMDVFIATYKGYGLWYHESEISVVGQRAAGVKSVSLKDDDEVVNGQVFDDLSEPSLALITQRGSCKRM
KLKDFEKTTRAKRGVIMLRELKNKAHRVKGFFALQDHDGQQICFQTANGNTHEIVPLTLKATDRYNNGSFVIDTDHEGEV
IHVWKQANYEKPFDE

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm. Note=Bipolarly localized [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=370, Percent_Identity=23.7837837837838, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI19913406, Length=170, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=5e-12,
Organism=Escherichia coli, GI1788562, Length=846, Percent_Identity=37.5886524822695, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1789396, Length=513, Percent_Identity=38.9863547758285, Blast_Score=352, Evalue=8e-98,
Organism=Caenorhabditis elegans, GI17535065, Length=167, Percent_Identity=31.1377245508982, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=167, Percent_Identity=29.3413173652695, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17136538, Length=379, Percent_Identity=24.802110817942, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013760
- InterPro:   IPR005741 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: 5.99.1.-

Molecular weight: Translated: 92532; Mature: 92401

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTEKYLDLPLEEVIGDRFGRYSKYIIQDRALPDARDGLKPVQRRILYAMHMERNTHDK
CCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCHH
HFRKSAKTVGTVIGNYHPHGDSSVYDAMVRLSQDWKVRNGLIEMHGNNGSIDGDPPAAMR
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEECCEEEEECCCCCCCCCCCHHHH
YTEARLSSISAELLRDIDKQTVDYIPNFDETTDEPVVLPAKFPNLLVNGSTGISAGYATD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCC
IPPHNLEEVMDAVILKIDKPEASIDDLMKLMKGPDFPTGGIIQGVEGIRKAYETGRGKII
CCCCCHHHHHHHHEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
VRGKAVVETMKGGREQIVVTEVPFEVNKATMVKKMDELRIDRKVEGIAEVRDETDRTGLR
EECHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
VVIELKKDANSEGVLNYLYKNTDLQITYHFNMVAIQDKTPKLLSLPSLLDAYIAHQKEVV
EEEEEECCCCCCCHHHEEECCCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
TRQITYDLNKANARAHIVEGLIKAISILDELIATIRASKDKGDAKAQIKAKYGFTEEQAE
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHH
AIVMLQLYRLTNTDIVELEKEAAELKEKIKEYESILSSDKKLFQTIKKELRQIKKKFADP
HEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCH
RRTVIEEKIEELKINIEVTVASEDVLVSVTRDGYLKRTSLRSYAASNEEELPVKDDDYPI
HHHHHHHHHHHEEEEEEEEEECCCEEEEEECCCCEEHHHHHHHHCCCCCCCCCCCCCCCE
SLLEVNTTDNLLLFTNKGKYILCPVHELPDIRWKDMGQHISNIVPLDKEEYLIQCMPVRD
EEEEECCCCCEEEEECCCCEEEEEHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCC
FKSAEGYLIFFTKNGMVKRSEFQLYDAQRRSKALIALNLKGDDEVVHVSHTNGNMDVFIA
CCCCCCEEEEEECCCCEECCHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCEEEEEE
TYKGYGLWYHESEISVVGQRAAGVKSVSLKDDDEVVNGQVFDDLSEPSLALITQRGSCKR
EECCCEEEEECCCHHEEEHHCCCCEEECCCCCCHHCCCHHHCCCCCCCEEEEECCCCCCC
MKLKDFEKTTRAKRGVIMLRELKNKAHRVKGFFALQDHDGQQICFQTANGNTHEIVPLTL
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCEEEEEEEE
KATDRYNNGSFVIDTDHEGEVIHVWKQANYEKPFDE
EECCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SQTEKYLDLPLEEVIGDRFGRYSKYIIQDRALPDARDGLKPVQRRILYAMHMERNTHDK
CCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCHH
HFRKSAKTVGTVIGNYHPHGDSSVYDAMVRLSQDWKVRNGLIEMHGNNGSIDGDPPAAMR
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEECCEEEEECCCCCCCCCCCHHHH
YTEARLSSISAELLRDIDKQTVDYIPNFDETTDEPVVLPAKFPNLLVNGSTGISAGYATD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCC
IPPHNLEEVMDAVILKIDKPEASIDDLMKLMKGPDFPTGGIIQGVEGIRKAYETGRGKII
CCCCCHHHHHHHHEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEE
VRGKAVVETMKGGREQIVVTEVPFEVNKATMVKKMDELRIDRKVEGIAEVRDETDRTGLR
EECHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
VVIELKKDANSEGVLNYLYKNTDLQITYHFNMVAIQDKTPKLLSLPSLLDAYIAHQKEVV
EEEEEECCCCCCCHHHEEECCCCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
TRQITYDLNKANARAHIVEGLIKAISILDELIATIRASKDKGDAKAQIKAKYGFTEEQAE
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHH
AIVMLQLYRLTNTDIVELEKEAAELKEKIKEYESILSSDKKLFQTIKKELRQIKKKFADP
HEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCH
RRTVIEEKIEELKINIEVTVASEDVLVSVTRDGYLKRTSLRSYAASNEEELPVKDDDYPI
HHHHHHHHHHHEEEEEEEEEECCCEEEEEECCCCEEHHHHHHHHCCCCCCCCCCCCCCCE
SLLEVNTTDNLLLFTNKGKYILCPVHELPDIRWKDMGQHISNIVPLDKEEYLIQCMPVRD
EEEEECCCCCEEEEECCCCEEEEEHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCC
FKSAEGYLIFFTKNGMVKRSEFQLYDAQRRSKALIALNLKGDDEVVHVSHTNGNMDVFIA
CCCCCCEEEEEECCCCEECCHHHHHHHHCCCCEEEEEEECCCCCEEEEEECCCCEEEEEE
TYKGYGLWYHESEISVVGQRAAGVKSVSLKDDDEVVNGQVFDDLSEPSLALITQRGSCKR
EECCCEEEEECCCHHEEEHHCCCCEEECCCCCCHHCCCHHHCCCCCCCEEEEECCCCCCC
MKLKDFEKTTRAKRGVIMLRELKNKAHRVKGFFALQDHDGQQICFQTANGNTHEIVPLTL
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCCCEEEEEEEE
KATDRYNNGSFVIDTDHEGEVIHVWKQANYEKPFDE
EECCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969507; 9539793; 9384377 [H]