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The map label for this gene is grlB [H]

Identifier: 23099148

GI number: 23099148

Start: 1732198

End: 1734153

Strand: Direct

Name: grlB [H]

Synonym: OB1693

Alternate gene names: 23099148

Gene position: 1732198-1734153 (Clockwise)

Preceding gene: 23099147

Following gene: 23099149

Centisome position: 47.71

GC content: 37.58

Gene sequence:

>1956_bases
GTGACGAGTGATTATCAATATTCAGACAGCTCCATACAAGTATTAGAGGGGTTAGATGCTGTTCGTAAAAGGCCAGGTAT
GTATATTGGAAGTACAGATATACGAGGACTTCATCACTTAGTGTATGAGATAGTAGATAATGCAGTAGATGAAGCACTTG
CTGGTTATGGTAAAGAAATTCAAGTAACTATACATAAAGATGAAAGTGTATCAGTAAAAGATAGTGGGCGAGGAATGCCT
ACAGGGATGCACCAAACCGGGAAGCCAACTACGGAAGTAATCTTCACAGTACTGCACGCTGGTGGCAAGTTTGGACAAGG
AAGTTATAAAACAAGTGGAGGACTTCATGGTGTTGGTGCTTCTGTCGTAAATGCATTATCTGAGTGGTTAGAAGTTACCA
CATTTCGTGATGGTTTCCAATATCAACAACGTTTTGAAAATGGTGGGAGACCGGTAACTACATTAGAAAAAAAAGGTCCT
ACTCGAAAAAAAGGATCAGTTATTCGCTTTAAGCCAGATCCAACTATTTTCTCAACAATTGTTTACAATTATGAAACGAT
ATCTGAACGACTACGTGAATCTGCCTTTTTATTAAAAGGTCTTAAAATAGAATTGATTGATGAAAGACATGATAAGCATG
ATGTATATGAGTATCCAGAAGGGCTAAAGTCTTTCGTAGATTATCTGAATGAAGAAAAAGATGCTTTACACTCCATTGTC
TTTATGGAAGGTGAACAACAAGAAATTGAAGTAGAGTTTGCTTTTCAATTTAATGATGGTTTTGCAGAAAATATGCTGTC
ATTTGTTAACCATGTGCGTACTCGAGATGGAGGAACGCATGAATCGGGAGCACGTACAGCAATTACTCGTACGGTTAATG
ATTATGCGAGAAGGCAAGGTCTATTAAAAGAAAAAGAGAAAAATCTAGAAGGTACAGATATACGAGAAGGATTAACAGGA
GTTGTATCTGTTCGAGTTCCAGAAGATAAACTTCAATTTGAAGGGCAAACAAAAGGAAAGCTCGGTACAACTGAAGCGAG
ATCAGCTGTAGATTCTGTCATCTCGGAACATCTAAATTATTTCTTAGAAGAAAATCCCGATATCTCTGGAATGCTAATTA
AAAAGTCAATAAAGGCAAAAGAAGCTAGAGAAGCAGCCAGAAAAGCAAGAGAAGAAGCAAGGACAGGAAAAAAACGGAAA
CGTAAAGATGCTTTACTAAGTGGAAAGCTTACGCCAGCACAATCCAGAAACCCGGAAAAAAATGAATTATACCTAGTGGA
GGGGGATTCTGCTGGAGGATCAGCCAAACAAGGAAGAGATCGTAAATTTCAAGCAGTATTACCACTAAGAGGTAAAGTAA
TCAATACAGAAAAAGCAAAATTACAAGATGTATTTAAGAATGAAGAAATTTCAACAATTATCCATACGATTGGCGCTGGT
GTTGGTGGCGATTTTAATCTTGATGATGTTCAATATGACAAAATAATCATCATGACTGATGCTGATACAGATGGGGCGCA
CATTCAAGTGTTATTACTTACCTTCTTTTATCGTTATATGCGTCCACTAGTAGAAGCAGGAAAAATCTTTATTGCATTAC
CTCCTTTATTTAAAGTATCAAAAGGAAAAGGCAAGCAAGAAAAGCTTCGTTATGCATGGGATGAACAAGAATTAAAAGTT
GCTATTCAAGAATTAAAACAAGGTTATACAATTCAACGTTATAAAGGTCTCGGTGAGATGAATGCGGACCAATTATGGGA
AACTACGATGAATCCAAGTTCTAGAACGCTCATTCGTGTATCCATTGATGACATTGCACGTGCAGAACGTAGGGTAACTA
CTTTAATGGGTGATAAAGTCGAGCCCCGCAGAAAGTGGATCGAAGGACATGTGAAATTTGGCCTTGAAGAGGATGCAACT
ATACTTGAGAACGAAAATATTCACACAGAAGAGTAA

Upstream 100 bases:

>100_bases
TATTAATGTAAAAGTAAAATTACATCGAGAGATATTTACAAAAAAGAACAGTTGTTCTATGATGATTGTTAAGGTAAGTT
GGGGAAGGAGAAGATGGCAC

Downstream 100 bases:

>100_bases
AGTGACTAATAGATGTTGTTCTTTTAGGTCCTTTTGAACACGCAATTATAGGGGGGATTCAATTTGTCACAAACAGAAAA
ATATCTCGATCTACCTCTAG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 651; Mature: 650

Protein sequence:

>651_residues
MTSDYQYSDSSIQVLEGLDAVRKRPGMYIGSTDIRGLHHLVYEIVDNAVDEALAGYGKEIQVTIHKDESVSVKDSGRGMP
TGMHQTGKPTTEVIFTVLHAGGKFGQGSYKTSGGLHGVGASVVNALSEWLEVTTFRDGFQYQQRFENGGRPVTTLEKKGP
TRKKGSVIRFKPDPTIFSTIVYNYETISERLRESAFLLKGLKIELIDERHDKHDVYEYPEGLKSFVDYLNEEKDALHSIV
FMEGEQQEIEVEFAFQFNDGFAENMLSFVNHVRTRDGGTHESGARTAITRTVNDYARRQGLLKEKEKNLEGTDIREGLTG
VVSVRVPEDKLQFEGQTKGKLGTTEARSAVDSVISEHLNYFLEENPDISGMLIKKSIKAKEAREAARKAREEARTGKKRK
RKDALLSGKLTPAQSRNPEKNELYLVEGDSAGGSAKQGRDRKFQAVLPLRGKVINTEKAKLQDVFKNEEISTIIHTIGAG
VGGDFNLDDVQYDKIIIMTDADTDGAHIQVLLLTFFYRYMRPLVEAGKIFIALPPLFKVSKGKGKQEKLRYAWDEQELKV
AIQELKQGYTIQRYKGLGEMNADQLWETTMNPSSRTLIRVSIDDIARAERRVTTLMGDKVEPRRKWIEGHVKFGLEEDAT
ILENENIHTEE

Sequences:

>Translated_651_residues
MTSDYQYSDSSIQVLEGLDAVRKRPGMYIGSTDIRGLHHLVYEIVDNAVDEALAGYGKEIQVTIHKDESVSVKDSGRGMP
TGMHQTGKPTTEVIFTVLHAGGKFGQGSYKTSGGLHGVGASVVNALSEWLEVTTFRDGFQYQQRFENGGRPVTTLEKKGP
TRKKGSVIRFKPDPTIFSTIVYNYETISERLRESAFLLKGLKIELIDERHDKHDVYEYPEGLKSFVDYLNEEKDALHSIV
FMEGEQQEIEVEFAFQFNDGFAENMLSFVNHVRTRDGGTHESGARTAITRTVNDYARRQGLLKEKEKNLEGTDIREGLTG
VVSVRVPEDKLQFEGQTKGKLGTTEARSAVDSVISEHLNYFLEENPDISGMLIKKSIKAKEAREAARKAREEARTGKKRK
RKDALLSGKLTPAQSRNPEKNELYLVEGDSAGGSAKQGRDRKFQAVLPLRGKVINTEKAKLQDVFKNEEISTIIHTIGAG
VGGDFNLDDVQYDKIIIMTDADTDGAHIQVLLLTFFYRYMRPLVEAGKIFIALPPLFKVSKGKGKQEKLRYAWDEQELKV
AIQELKQGYTIQRYKGLGEMNADQLWETTMNPSSRTLIRVSIDDIARAERRVTTLMGDKVEPRRKWIEGHVKFGLEEDAT
ILENENIHTEE
>Mature_650_residues
TSDYQYSDSSIQVLEGLDAVRKRPGMYIGSTDIRGLHHLVYEIVDNAVDEALAGYGKEIQVTIHKDESVSVKDSGRGMPT
GMHQTGKPTTEVIFTVLHAGGKFGQGSYKTSGGLHGVGASVVNALSEWLEVTTFRDGFQYQQRFENGGRPVTTLEKKGPT
RKKGSVIRFKPDPTIFSTIVYNYETISERLRESAFLLKGLKIELIDERHDKHDVYEYPEGLKSFVDYLNEEKDALHSIVF
MEGEQQEIEVEFAFQFNDGFAENMLSFVNHVRTRDGGTHESGARTAITRTVNDYARRQGLLKEKEKNLEGTDIREGLTGV
VSVRVPEDKLQFEGQTKGKLGTTEARSAVDSVISEHLNYFLEENPDISGMLIKKSIKAKEAREAARKAREEARTGKKRKR
KDALLSGKLTPAQSRNPEKNELYLVEGDSAGGSAKQGRDRKFQAVLPLRGKVINTEKAKLQDVFKNEEISTIIHTIGAGV
GGDFNLDDVQYDKIIIMTDADTDGAHIQVLLLTFFYRYMRPLVEAGKIFIALPPLFKVSKGKGKQEKLRYAWDEQELKVA
IQELKQGYTIQRYKGLGEMNADQLWETTMNPSSRTLIRVSIDDIARAERRVTTLMGDKVEPRRKWIEGHVKFGLEEDATI
LENENIHTEE

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=606, Percent_Identity=26.0726072607261, Blast_Score=120, Evalue=6e-27,
Organism=Homo sapiens, GI19913408, Length=619, Percent_Identity=25.8481421647819, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI48994957, Length=566, Percent_Identity=52.1201413427562, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1789408, Length=639, Percent_Identity=37.245696400626, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=656, Percent_Identity=22.7134146341463, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI212645845, Length=542, Percent_Identity=24.9077490774908, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI212645657, Length=201, Percent_Identity=30.3482587064677, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=654, Percent_Identity=26.1467889908257, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17136538, Length=670, Percent_Identity=25.0746268656716, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 73267; Mature: 73136

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDYQYSDSSIQVLEGLDAVRKRPGMYIGSTDIRGLHHLVYEIVDNAVDEALAGYGKEI
CCCCCCCCCCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
QVTIHKDESVSVKDSGRGMPTGMHQTGKPTTEVIFTVLHAGGKFGQGSYKTSGGLHGVGA
EEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
SVVNALSEWLEVTTFRDGFQYQQRFENGGRPVTTLEKKGPTRKKGSVIRFKPDPTIFSTI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEECCCCCEEHHH
VYNYETISERLRESAFLLKGLKIELIDERHDKHDVYEYPEGLKSFVDYLNEEKDALHSIV
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEE
FMEGEQQEIEVEFAFQFNDGFAENMLSFVNHVRTRDGGTHESGARTAITRTVNDYARRQG
EECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
LLKEKEKNLEGTDIREGLTGVVSVRVPEDKLQFEGQTKGKLGTTEARSAVDSVISEHLNY
HHHHHHCCCCCCHHHHCCCEEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FLEENPDISGMLIKKSIKAKEAREAARKAREEARTGKKRKRKDALLSGKLTPAQSRNPEK
HHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NELYLVEGDSAGGSAKQGRDRKFQAVLPLRGKVINTEKAKLQDVFKNEEISTIIHTIGAG
CEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHCC
VGGDFNLDDVQYDKIIIMTDADTDGAHIQVLLLTFFYRYMRPLVEAGKIFIALPPLFKVS
CCCCCCCCCEEECEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
KGKGKQEKLRYAWDEQELKVAIQELKQGYTIQRYKGLGEMNADQLWETTMNPSSRTLIRV
CCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEHHHCCCCCCCHHHHHHHCCCCCCCEEEEE
SIDDIARAERRVTTLMGDKVEPRRKWIEGHVKFGLEEDATILENENIHTEE
EHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
TSDYQYSDSSIQVLEGLDAVRKRPGMYIGSTDIRGLHHLVYEIVDNAVDEALAGYGKEI
CCCCCCCCCHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
QVTIHKDESVSVKDSGRGMPTGMHQTGKPTTEVIFTVLHAGGKFGQGSYKTSGGLHGVGA
EEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
SVVNALSEWLEVTTFRDGFQYQQRFENGGRPVTTLEKKGPTRKKGSVIRFKPDPTIFSTI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEECCCCCEEHHH
VYNYETISERLRESAFLLKGLKIELIDERHDKHDVYEYPEGLKSFVDYLNEEKDALHSIV
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEE
FMEGEQQEIEVEFAFQFNDGFAENMLSFVNHVRTRDGGTHESGARTAITRTVNDYARRQG
EECCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
LLKEKEKNLEGTDIREGLTGVVSVRVPEDKLQFEGQTKGKLGTTEARSAVDSVISEHLNY
HHHHHHCCCCCCHHHHCCCEEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FLEENPDISGMLIKKSIKAKEAREAARKAREEARTGKKRKRKDALLSGKLTPAQSRNPEK
HHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NELYLVEGDSAGGSAKQGRDRKFQAVLPLRGKVINTEKAKLQDVFKNEEISTIIHTIGAG
CEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHCC
VGGDFNLDDVQYDKIIIMTDADTDGAHIQVLLLTFFYRYMRPLVEAGKIFIALPPLFKVS
CCCCCCCCCEEECEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
KGKGKQEKLRYAWDEQELKVAIQELKQGYTIQRYKGLGEMNADQLWETTMNPSSRTLIRV
CCCCHHHHHHHCCCHHHHHHHHHHHHCCCEEHHHCCCCCCCHHHHHHHCCCCCCCEEEEE
SIDDIARAERRVTTLMGDKVEPRRKWIEGHVKFGLEEDATILENENIHTEE
EHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969507; 9539793; 9384377 [H]