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The map label for this gene is liaS [H]

Identifier: 23098616

GI number: 23098616

Start: 1210939

End: 1212009

Strand: Direct

Name: liaS [H]

Synonym: OB1161

Alternate gene names: 23098616

Gene position: 1210939-1212009 (Clockwise)

Preceding gene: 23098615

Following gene: 23098617

Centisome position: 33.35

GC content: 32.21

Gene sequence:

>1071_bases
ATGCATAATCGATTAAAAGGAATTCGATTCATATTTATTCGTTCCCATATTTTTGTACTATTCCTTTCTTCTGTTATCTT
ACTTTCCTTATTGTTATCGGTTCATGTTTTATTTGAACCGGAATGGTTAACAACGGAAAGTGTCTTTCTCTTTATTATTT
CACATTTTGTTATCGGTTTTCCATTAGCGTTTTACGCTGGGTTTAATGGTAATGGCAATTATTTAAAGACTAGTTTTGAT
GCTATTACATTACTAATCAAACAATATGCAAACGGAAATTATCAGTCATCTATTCAACTACTGGATAATGAAGATGATGA
GATTCATAGAGCATCTAATTCTTTAAATGAATTAGGGAAAAAATTACAAGATCAAGTGAAATCATTACAAAAAATGGCGG
ATGAAAAATCAGAATTAGCGAAGTCAGCACATAAAACAGCTGTGATTGAAGAACGCCAGCGAATTGCAAGGGATTTACAT
GATGCGGTAAGTCAAGAATTATTCGCACTTGCAATGTTATCCGAAGCGGCTATAAAACAATTAGATTTAAAACCAGAATT
AGCTAAAGCTCAAATGCTAGAAGTCTCTCAATCTGCTCATCAAGCACAAACAGAAATGAGAGCATTATTATTACATTTAC
GCCCAGTATATCTAAGTGGTGAATCTTTAATTGAAGGGTTACATAAATTAATTAATGAATTGAAGCAAAAAAGCAATCTA
CAGTTTACACTCAAATTTGATGATCAACTTGAAATGCAAGAAACGATTGAAGAACATATTTTTCGAATAGTACCAGAATC
ATTATCTAATATTCTAAGGCATGCAAACGCAACCGAGGTATATGTGGAAATTTCGAAAAAATCAGAAGAAATCTTTATTT
TAATTGAAGATAATGGTGAAGGATTTGATATGACAGGGACTAGTGGGAAAAAGACTTCTTATGGATTAAAAACAATGAAA
GAGCGTAGTGAAGAAATTGGTGGAACATTTACTGTCCGATCAAATGTAGATGAAGGCACTTATATCCAAATTAGAATACC
ATATGAAGAAGAATGGAGGAGAAGAACATGA

Upstream 100 bases:

>100_bases
ATGAATCAGAAAATTATCCAAATGCGAGTAAAAAAGTAGACTTGTTTATTCGTTTAAAAGCAGGATCTATACGTGTGGAA
AGAATATAAGGGGGATCCAG

Downstream 100 bases:

>100_bases
TTCGTGTCGTAGTTATTGATGACCATGATGTTGTTCGAAAAGGGATTGTAGCTTATTTGCAAACGGAAGAGGGGATAGAA
ATTGTTGGCCAGGCTTCTAG

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MHNRLKGIRFIFIRSHIFVLFLSSVILLSLLLSVHVLFEPEWLTTESVFLFIISHFVIGFPLAFYAGFNGNGNYLKTSFD
AITLLIKQYANGNYQSSIQLLDNEDDEIHRASNSLNELGKKLQDQVKSLQKMADEKSELAKSAHKTAVIEERQRIARDLH
DAVSQELFALAMLSEAAIKQLDLKPELAKAQMLEVSQSAHQAQTEMRALLLHLRPVYLSGESLIEGLHKLINELKQKSNL
QFTLKFDDQLEMQETIEEHIFRIVPESLSNILRHANATEVYVEISKKSEEIFILIEDNGEGFDMTGTSGKKTSYGLKTMK
ERSEEIGGTFTVRSNVDEGTYIQIRIPYEEEWRRRT

Sequences:

>Translated_356_residues
MHNRLKGIRFIFIRSHIFVLFLSSVILLSLLLSVHVLFEPEWLTTESVFLFIISHFVIGFPLAFYAGFNGNGNYLKTSFD
AITLLIKQYANGNYQSSIQLLDNEDDEIHRASNSLNELGKKLQDQVKSLQKMADEKSELAKSAHKTAVIEERQRIARDLH
DAVSQELFALAMLSEAAIKQLDLKPELAKAQMLEVSQSAHQAQTEMRALLLHLRPVYLSGESLIEGLHKLINELKQKSNL
QFTLKFDDQLEMQETIEEHIFRIVPESLSNILRHANATEVYVEISKKSEEIFILIEDNGEGFDMTGTSGKKTSYGLKTMK
ERSEEIGGTFTVRSNVDEGTYIQIRIPYEEEWRRRT
>Mature_356_residues
MHNRLKGIRFIFIRSHIFVLFLSSVILLSLLLSVHVLFEPEWLTTESVFLFIISHFVIGFPLAFYAGFNGNGNYLKTSFD
AITLLIKQYANGNYQSSIQLLDNEDDEIHRASNSLNELGKKLQDQVKSLQKMADEKSELAKSAHKTAVIEERQRIARDLH
DAVSQELFALAMLSEAAIKQLDLKPELAKAQMLEVSQSAHQAQTEMRALLLHLRPVYLSGESLIEGLHKLINELKQKSNL
QFTLKFDDQLEMQETIEEHIFRIVPESLSNILRHANATEVYVEISKKSEEIFILIEDNGEGFDMTGTSGKKTSYGLKTMK
ERSEEIGGTFTVRSNVDEGTYIQIRIPYEEEWRRRT

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=200, Percent_Identity=28, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788812, Length=199, Percent_Identity=28.643216080402, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87082311, Length=240, Percent_Identity=27.0833333333333, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40752; Mature: 40752

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNRLKGIRFIFIRSHIFVLFLSSVILLSLLLSVHVLFEPEWLTTESVFLFIISHFVIGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
PLAFYAGFNGNGNYLKTSFDAITLLIKQYANGNYQSSIQLLDNEDDEIHRASNSLNELGK
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHEEEECCCCHHHHHHHHHHHHHHH
KLQDQVKSLQKMADEKSELAKSAHKTAVIEERQRIARDLHDAVSQELFALAMLSEAAIKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLKPELAKAQMLEVSQSAHQAQTEMRALLLHLRPVYLSGESLIEGLHKLINELKQKSNL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHCCC
QFTLKFDDQLEMQETIEEHIFRIVPESLSNILRHANATEVYVEISKKSEEIFILIEDNGE
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCC
GFDMTGTSGKKTSYGLKTMKERSEEIGGTFTVRSNVDEGTYIQIRIPYEEEWRRRT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCHHHHCCCC
>Mature Secondary Structure
MHNRLKGIRFIFIRSHIFVLFLSSVILLSLLLSVHVLFEPEWLTTESVFLFIISHFVIGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
PLAFYAGFNGNGNYLKTSFDAITLLIKQYANGNYQSSIQLLDNEDDEIHRASNSLNELGK
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCHHHHEEEECCCCHHHHHHHHHHHHHHH
KLQDQVKSLQKMADEKSELAKSAHKTAVIEERQRIARDLHDAVSQELFALAMLSEAAIKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDLKPELAKAQMLEVSQSAHQAQTEMRALLLHLRPVYLSGESLIEGLHKLINELKQKSNL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHCCC
QFTLKFDDQLEMQETIEEHIFRIVPESLSNILRHANATEVYVEISKKSEEIFILIEDNGE
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCC
GFDMTGTSGKKTSYGLKTMKERSEEIGGTFTVRSNVDEGTYIQIRIPYEEEWRRRT
CCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]