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The map label for this gene is liaR [H]

Identifier: 23098617

GI number: 23098617

Start: 1212006

End: 1212635

Strand: Direct

Name: liaR [H]

Synonym: OB1162

Alternate gene names: 23098617

Gene position: 1212006-1212635 (Clockwise)

Preceding gene: 23098616

Following gene: 23098618

Centisome position: 33.38

GC content: 33.97

Gene sequence:

>630_bases
ATGATTCGTGTCGTAGTTATTGATGACCATGATGTTGTTCGAAAAGGGATTGTAGCTTATTTGCAAACGGAAGAGGGGAT
AGAAATTGTTGGCCAGGCTTCTAGTGGTTTTGAAGGAGTTGAAATTGTTTTAAAAGAAAAACCAGATGTAGTCTTAATGG
ATTTAATTATGGAAAATGGAACGGGTATTGAAGCAACAAAATTGATAATTAATGATTATCCAGATTGTAAAGTAATCATA
TTAACTAGTTTTTATGATGATGAGAAAGTCTTTCCAGCTATTGAAGCAGGAGCGTTCAGTTATATTTTAAAGACTTCAAC
AGCAAATGAAATTGCCAATGCTATTAAGAAAGCTGCGTCAGGAGAAAATGTAATTGAACCAAAAGTGGCGAGTAAAATGA
TGACTAATTTTCAATCACAGAAGAAGAAATTACATGATGACTTAACAGAGAGGGAAAGAGAAGTGCTTCTATGTATTGGA
GAAGGGCTAACGAACCAAGAAATAGGTGAAAAACTGTATATTGGTGTGAAAACAGTAAAAACCCATGTTAGCAATATCCT
TTCGAAATTAGATGTACATGACAGAACACAGGCAGCTGTCTATGTTCACCGAAACAAATTATTAAAATAA

Upstream 100 bases:

>100_bases
GTGAAGAAATTGGTGGAACATTTACTGTCCGATCAAATGTAGATGAAGGCACTTATATCCAAATTAGAATACCATATGAA
GAAGAATGGAGGAGAAGAAC

Downstream 100 bases:

>100_bases
TTTGTTTCAAATTTTTGTCAAATAAGTCTAACTAATTGTATTTCCATGTTCGGTTTCTGTATAATACAACTGTACTTCAA
AAGAGAGGACAAGGCATTTA

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MIRVVVIDDHDVVRKGIVAYLQTEEGIEIVGQASSGFEGVEIVLKEKPDVVLMDLIMENGTGIEATKLIINDYPDCKVII
LTSFYDDEKVFPAIEAGAFSYILKTSTANEIANAIKKAASGENVIEPKVASKMMTNFQSQKKKLHDDLTEREREVLLCIG
EGLTNQEIGEKLYIGVKTVKTHVSNILSKLDVHDRTQAAVYVHRNKLLK

Sequences:

>Translated_209_residues
MIRVVVIDDHDVVRKGIVAYLQTEEGIEIVGQASSGFEGVEIVLKEKPDVVLMDLIMENGTGIEATKLIINDYPDCKVII
LTSFYDDEKVFPAIEAGAFSYILKTSTANEIANAIKKAASGENVIEPKVASKMMTNFQSQKKKLHDDLTEREREVLLCIG
EGLTNQEIGEKLYIGVKTVKTHVSNILSKLDVHDRTQAAVYVHRNKLLK
>Mature_209_residues
MIRVVVIDDHDVVRKGIVAYLQTEEGIEIVGQASSGFEGVEIVLKEKPDVVLMDLIMENGTGIEATKLIINDYPDCKVII
LTSFYDDEKVFPAIEAGAFSYILKTSTANEIANAIKKAASGENVIEPKVASKMMTNFQSQKKKLHDDLTEREREVLLCIG
EGLTNQEIGEKLYIGVKTVKTHVSNILSKLDVHDRTQAAVYVHRNKLLK

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=195, Percent_Identity=34.8717948717949, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788521, Length=207, Percent_Identity=31.4009661835749, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1787473, Length=209, Percent_Identity=31.1004784688995, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=29, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1786747, Length=194, Percent_Identity=28.3505154639175, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788712, Length=206, Percent_Identity=27.6699029126214, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=35.5769230769231, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23217; Mature: 23217

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVVVIDDHDVVRKGIVAYLQTEEGIEIVGQASSGFEGVEIVLKEKPDVVLMDLIMENG
CEEEEEECCHHHHHHHHHHEEECCCCEEEEECCCCCCCCEEEEEECCCCEEEEEEHHCCC
TGIEATKLIINDYPDCKVIILTSFYDDEKVFPAIEAGAFSYILKTSTANEIANAIKKAAS
CCCEEEEEEECCCCCCEEEEEEEECCCCCCCCHHHCCHHEEEEECCCHHHHHHHHHHHCC
GENVIEPKVASKMMTNFQSQKKKLHDDLTEREREVLLCIGEGLTNQEIGEKLYIGVKTVK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHCCEEEEHHHHHH
THVSNILSKLDVHDRTQAAVYVHRNKLLK
HHHHHHHHHCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MIRVVVIDDHDVVRKGIVAYLQTEEGIEIVGQASSGFEGVEIVLKEKPDVVLMDLIMENG
CEEEEEECCHHHHHHHHHHEEECCCCEEEEECCCCCCCCEEEEEECCCCEEEEEEHHCCC
TGIEATKLIINDYPDCKVIILTSFYDDEKVFPAIEAGAFSYILKTSTANEIANAIKKAAS
CCCEEEEEEECCCCCCEEEEEEEECCCCCCCCHHHCCHHEEEEECCCHHHHHHHHHHHCC
GENVIEPKVASKMMTNFQSQKKKLHDDLTEREREVLLCIGEGLTNQEIGEKLYIGVKTVK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHCCEEEEHHHHHH
THVSNILSKLDVHDRTQAAVYVHRNKLLK
HHHHHHHHHCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]