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The map label for this gene is dhbE [H]

Identifier: 23098411

GI number: 23098411

Start: 1002550

End: 1004169

Strand: Direct

Name: dhbE [H]

Synonym: OB0956

Alternate gene names: 23098411

Gene position: 1002550-1004169 (Clockwise)

Preceding gene: 23098410

Following gene: 23098412

Centisome position: 27.61

GC content: 36.42

Gene sequence:

>1620_bases
ATGCTAGAAGGGTATAAGCCATGGCCAGAGAATCTTAGAAAGTACTATCAAGAAACTGGTTGCTGGACGGGAGAGACTTT
CGGAGGAATATTAGAGCAACAAGCTAGAGACAGAAAAGACAGTATTGCGATTACTGATGGAGAGAAATCCATTACGTATC
AGCAATTAGATGAAAGAGTAAACAAACTAGCTGCAGGATTCATGAAATTAGGTATAGAGAAAGAAGATAGAGTAGTTCTT
CAACTACCAAATACTATTGAATTTTTTGAGGTTTGTTTCGCGTTATTTCGAATTGGTGCTTTACCTGTTTTTGCTCTACC
GCTGCATCGAAAAATGGAAATTAGTTATTTCTGTGAATTTACAGCAGCTAAAGCGTATATCATTCCAGATGTATTCGATA
AATTTGACTACAGAGATCTAGCAAATGAAATAAATAACTCTATTCCTACTCTGGAGCATACGATTGTTGCAGGTGAAACA
GATAATTTTATTTCATTAGAAGAATTATATATGGATGATTCAGCATCTTTTCCAACTATTACTGGAAGTGATTTAGCTTT
TTTCCAATTATCAGGTGGAAGTACTGGATTACCTAAACTAATTCCGCGTACACATGATGAGTATATTTATAGTCTTCGGA
AAAGTGTGGAAATATGTCGAATTAGTCAAGATACGAATTTCTTAGCGGTTCTACCTGTTGCACATAATTTTACGATGAGT
TCACCAGGAGTTTTTGGTGTCATATATGCTGGTGGGAAAATTATTCTAAGTAAATATCCTAGTCCTGATGTGGCTTTTCC
GTTAATAGAAAAGGAGAAAGTAGATTTCACATCATTGGTTCCACCATTAGCGATTGTATGGTTACAAGCGCAGAAAAGGA
CAAAAGCAGATTTACGTTCGTTGGAAGTCATTCAAGTTGGTGGAGCAAAATGCAGTGTTGAAGTTGCGAAACAAATTGGC
CCGGCGTTTCACTGTAAATTACAGCAAGTATTTGGTATGGCAGAAGGCCTAGTAAATTATACGAGATTAGATGACGACGA
TGAAGTAGTTATTCATACACAAGGCAAACCGATGTCAGCGTATGATGAAATTCGTATTGTGGATGAAGAAGATAATGAGC
TACCAGTAGGAGAAACAGGACAACTTCAAGCGAGAGGTCCATATACCATCATTGGTTATTTCAATGTAGAAGACCATAAT
GCAAAAGCTTTTACTTCGGATGGTTTTTATCGCACTGGAGATTTAGTCAAACTAACGGAGGAAGGGTATCTAATCGTTGA
AGGAAGAGATAAGGATCAAATTAATCGTGGTGGCGAAAAAATTGCCGCAGAAGAGGTAGAAAATTATATCCTATCACTTT
CAGGCATACATGATGTAGCAATGGTGAGTATGCCAGACAAATTTCTTGGAGAAAGGTCTTGTGCCTTTGTCATAAAAAAT
GATGAAGAATTAACGAGCAATGACATAAAGGATTTCTTACAGCATAAAGGAATAGCTAATTTTAAAATCCCAGATCGGAT
AGAATTCGTGGATCGTTTTCCATATACAGCACTAGGTAAGGTGAGTAAGAAAAACTTAAGAGCAATGATAAAAGAAAAAA
TCACACAAAAAAGTTATTAA

Upstream 100 bases:

>100_bases
ATTCAGTCCCAGAAGAAGAAAAAAACGAAACGGCTGCGAAGTTACAAACGATGCTACGTGTATTTGGAATAGATTTTTAC
GAAGAGGAAGGGAATGGATC

Downstream 100 bases:

>100_bases
GGAGAGATAAATAATGTCAATACCGTCTATAGCAAGTTATACATTACCAACAGAAAATGAATTACCAAAAAGTAAAGTGG
ATTGGAAAGCAGAGGCAAGC

Product: 2,3-dihydroxybenzoate-AMP ligase

Products: NA

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MLEGYKPWPENLRKYYQETGCWTGETFGGILEQQARDRKDSIAITDGEKSITYQQLDERVNKLAAGFMKLGIEKEDRVVL
QLPNTIEFFEVCFALFRIGALPVFALPLHRKMEISYFCEFTAAKAYIIPDVFDKFDYRDLANEINNSIPTLEHTIVAGET
DNFISLEELYMDDSASFPTITGSDLAFFQLSGGSTGLPKLIPRTHDEYIYSLRKSVEICRISQDTNFLAVLPVAHNFTMS
SPGVFGVIYAGGKIILSKYPSPDVAFPLIEKEKVDFTSLVPPLAIVWLQAQKRTKADLRSLEVIQVGGAKCSVEVAKQIG
PAFHCKLQQVFGMAEGLVNYTRLDDDDEVVIHTQGKPMSAYDEIRIVDEEDNELPVGETGQLQARGPYTIIGYFNVEDHN
AKAFTSDGFYRTGDLVKLTEEGYLIVEGRDKDQINRGGEKIAAEEVENYILSLSGIHDVAMVSMPDKFLGERSCAFVIKN
DEELTSNDIKDFLQHKGIANFKIPDRIEFVDRFPYTALGKVSKKNLRAMIKEKITQKSY

Sequences:

>Translated_539_residues
MLEGYKPWPENLRKYYQETGCWTGETFGGILEQQARDRKDSIAITDGEKSITYQQLDERVNKLAAGFMKLGIEKEDRVVL
QLPNTIEFFEVCFALFRIGALPVFALPLHRKMEISYFCEFTAAKAYIIPDVFDKFDYRDLANEINNSIPTLEHTIVAGET
DNFISLEELYMDDSASFPTITGSDLAFFQLSGGSTGLPKLIPRTHDEYIYSLRKSVEICRISQDTNFLAVLPVAHNFTMS
SPGVFGVIYAGGKIILSKYPSPDVAFPLIEKEKVDFTSLVPPLAIVWLQAQKRTKADLRSLEVIQVGGAKCSVEVAKQIG
PAFHCKLQQVFGMAEGLVNYTRLDDDDEVVIHTQGKPMSAYDEIRIVDEEDNELPVGETGQLQARGPYTIIGYFNVEDHN
AKAFTSDGFYRTGDLVKLTEEGYLIVEGRDKDQINRGGEKIAAEEVENYILSLSGIHDVAMVSMPDKFLGERSCAFVIKN
DEELTSNDIKDFLQHKGIANFKIPDRIEFVDRFPYTALGKVSKKNLRAMIKEKITQKSY
>Mature_539_residues
MLEGYKPWPENLRKYYQETGCWTGETFGGILEQQARDRKDSIAITDGEKSITYQQLDERVNKLAAGFMKLGIEKEDRVVL
QLPNTIEFFEVCFALFRIGALPVFALPLHRKMEISYFCEFTAAKAYIIPDVFDKFDYRDLANEINNSIPTLEHTIVAGET
DNFISLEELYMDDSASFPTITGSDLAFFQLSGGSTGLPKLIPRTHDEYIYSLRKSVEICRISQDTNFLAVLPVAHNFTMS
SPGVFGVIYAGGKIILSKYPSPDVAFPLIEKEKVDFTSLVPPLAIVWLQAQKRTKADLRSLEVIQVGGAKCSVEVAKQIG
PAFHCKLQQVFGMAEGLVNYTRLDDDDEVVIHTQGKPMSAYDEIRIVDEEDNELPVGETGQLQARGPYTIIGYFNVEDHN
AKAFTSDGFYRTGDLVKLTEEGYLIVEGRDKDQINRGGEKIAAEEVENYILSLSGIHDVAMVSMPDKFLGERSCAFVIKN
DEELTSNDIKDFLQHKGIANFKIPDRIEFVDRFPYTALGKVSKKNLRAMIKEKITQKSY

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=550, Percent_Identity=24.5454545454545, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI38505220, Length=506, Percent_Identity=24.3083003952569, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI28416953, Length=555, Percent_Identity=24.3243243243243, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI115511026, Length=515, Percent_Identity=23.8834951456311, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI157311624, Length=503, Percent_Identity=24.6520874751491, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI157311622, Length=503, Percent_Identity=24.6520874751491, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI122937307, Length=508, Percent_Identity=25.1968503937008, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI58082049, Length=506, Percent_Identity=24.3083003952569, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI187761345, Length=546, Percent_Identity=23.2600732600733, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI187761343, Length=546, Percent_Identity=23.2600732600733, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI42544132, Length=483, Percent_Identity=22.9813664596273, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI195947378, Length=397, Percent_Identity=22.1662468513854, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786810, Length=534, Percent_Identity=44.7565543071161, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI145693145, Length=540, Percent_Identity=31.6666666666667, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1788107, Length=546, Percent_Identity=26.5567765567766, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI221142682, Length=486, Percent_Identity=25.7201646090535, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1786529, Length=546, Percent_Identity=24.3589743589744, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789201, Length=361, Percent_Identity=23.2686980609418, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1790505, Length=585, Percent_Identity=22.7350427350427, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=530, Percent_Identity=28.3018867924528, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71994694, Length=530, Percent_Identity=28.3018867924528, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI71994690, Length=530, Percent_Identity=28.3018867924528, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17560308, Length=522, Percent_Identity=26.0536398467433, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17538037, Length=519, Percent_Identity=26.2042389210019, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=527, Percent_Identity=26.1859582542694, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17559526, Length=528, Percent_Identity=24.4318181818182, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=512, Percent_Identity=23.2421875, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=539, Percent_Identity=25.417439703154, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=519, Percent_Identity=24.8554913294798, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=495, Percent_Identity=23.2323232323232, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17556356, Length=498, Percent_Identity=25.5020080321285, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=362, Percent_Identity=25.9668508287293, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=522, Percent_Identity=20.4980842911877, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=540, Percent_Identity=22.4074074074074, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71982997, Length=540, Percent_Identity=22.4074074074074, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=521, Percent_Identity=23.2245681381958, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24581924, Length=553, Percent_Identity=26.4014466546112, Blast_Score=161, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19922652, Length=529, Percent_Identity=24.007561436673, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24656500, Length=510, Percent_Identity=24.3137254901961, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21355181, Length=537, Percent_Identity=23.2774674115456, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI18859661, Length=517, Percent_Identity=22.6305609284333, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21356947, Length=528, Percent_Identity=21.5909090909091, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648260, Length=524, Percent_Identity=22.3282442748092, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20130357, Length=545, Percent_Identity=23.8532110091743, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648253, Length=547, Percent_Identity=22.6691042047532, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648255, Length=547, Percent_Identity=23.0347349177331, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653035, Length=345, Percent_Identity=23.4782608695652, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648257, Length=526, Percent_Identity=21.8631178707224, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356441, Length=318, Percent_Identity=22.3270440251572, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI161076582, Length=362, Percent_Identity=23.2044198895028, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281365686, Length=322, Percent_Identity=22.6708074534161, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.-

Molecular weight: Translated: 60681; Mature: 60681

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEGYKPWPENLRKYYQETGCWTGETFGGILEQQARDRKDSIAITDGEKSITYQQLDERV
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHH
NKLAAGFMKLGIEKEDRVVLQLPNTIEFFEVCFALFRIGALPVFALPLHRKMEISYFCEF
HHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEEE
TAAKAYIIPDVFDKFDYRDLANEINNSIPTLEHTIVAGETDNFISLEELYMDDSASFPTI
CCCCEEECHHHHHHCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHCCCCCCCCEE
TGSDLAFFQLSGGSTGLPKLIPRTHDEYIYSLRKSVEICRISQDTNFLAVLPVAHNFTMS
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCCC
SPGVFGVIYAGGKIILSKYPSPDVAFPLIEKEKVDFTSLVPPLAIVWLQAQKRTKADLRS
CCCEEEEEEECCEEEEECCCCCCCEECCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHC
LEVIQVGGAKCSVEVAKQIGPAFHCKLQQVFGMAEGLVNYTRLDDDDEVVIHTQGKPMSA
EEEEEECCCEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
YDEIRIVDEEDNELPVGETGQLQARGPYTIIGYFNVEDHNAKAFTSDGFYRTGDLVKLTE
CCCEEEEECCCCCCCCCCCCCEEECCCEEEEEEEEECCCCCEEEECCCCEECCCEEEEEC
EGYLIVEGRDKDQINRGGEKIAAEEVENYILSLSGIHDVAMVSMPDKFLGERSCAFVIKN
CCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCEEEEEEC
DEELTSNDIKDFLQHKGIANFKIPDRIEFVDRFPYTALGKVSKKNLRAMIKEKITQKSY
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLEGYKPWPENLRKYYQETGCWTGETFGGILEQQARDRKDSIAITDGEKSITYQQLDERV
CCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHH
NKLAAGFMKLGIEKEDRVVLQLPNTIEFFEVCFALFRIGALPVFALPLHRKMEISYFCEF
HHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEEE
TAAKAYIIPDVFDKFDYRDLANEINNSIPTLEHTIVAGETDNFISLEELYMDDSASFPTI
CCCCEEECHHHHHHCCHHHHHHHHCCCCCCCEEEEEECCCCCCEEHHHHHCCCCCCCCEE
TGSDLAFFQLSGGSTGLPKLIPRTHDEYIYSLRKSVEICRISQDTNFLAVLPVAHNFTMS
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCEEEEEEEECCCCCC
SPGVFGVIYAGGKIILSKYPSPDVAFPLIEKEKVDFTSLVPPLAIVWLQAQKRTKADLRS
CCCEEEEEEECCEEEEECCCCCCCEECCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHC
LEVIQVGGAKCSVEVAKQIGPAFHCKLQQVFGMAEGLVNYTRLDDDDEVVIHTQGKPMSA
EEEEEECCCEEHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
YDEIRIVDEEDNELPVGETGQLQARGPYTIIGYFNVEDHNAKAFTSDGFYRTGDLVKLTE
CCCEEEEECCCCCCCCCCCCCEEECCCEEEEEEEEECCCCCEEEECCCCEECCCEEEEEC
EGYLIVEGRDKDQINRGGEKIAAEEVENYILSLSGIHDVAMVSMPDKFLGERSCAFVIKN
CCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCCEEEEEEC
DEELTSNDIKDFLQHKGIANFKIPDRIEFVDRFPYTALGKVSKKNLRAMIKEKITQKSY
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]