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The map label for this gene is hisF

Identifier: 23098002

GI number: 23098002

Start: 580015

End: 580779

Strand: Reverse

Name: hisF

Synonym: OB0547

Alternate gene names: 23098002

Gene position: 580779-580015 (Counterclockwise)

Preceding gene: 23098003

Following gene: 23098001

Centisome position: 16.0

GC content: 39.48

Gene sequence:

>765_bases
ATGCTCGCTAAACGAATTATTCCTTGCCTCGATGTTGATAACGGGCGAGTAGTTAAGGGAAAGAAATTCTTAGATATCCA
AGATGTTGCTGATCCGGTTGAATTAGCAAAACGCTATAATGATGAAGGTGCTGATGAACTTGTCTTCTATGATATTACGG
CTTCCAATGAGCAGCGGGGAATATTTTTAGATGTTGTTGAAAAGGTAGCAAAAGAAATCGCTATCCCTTTTATGGTAGGT
GGAGGAATCCGTACAACTAAAGATATCCATCAAGTTCTTCGGTCTGGAGCTGATAAAGTATCAATAAACAGTGCGGCTGT
TCAACGTCCTGAGCTTATTTTCGAATCAGCACAAAAATTCGGAAGCCAGTGTACGGTCCTATCTATTGATGCAAAAGAGA
TCGCAGTTGGAAAGTGGAATGTTTTTATCAATGGAGGGAGAAAGGATACTGGAATTGATGCTATTGAATGGGCGAAAAAA
GGTGAATCTTATGGTGCTGGTGAAATCGTGGTAAATGCTATGGATGCAGATGGAGAAAAAAATGGCTATAATCTCCCCTT
AACAACAGCTATAGCAACAGCAGTTAATATACCAGTAATCGCTAGTGGCGGAGCTGGAAATATCCAACATTTCAAAGATG
TACTTTCACATGAAATAGATGCCGCCCTAGCAGCTTCTGTTTTTCACTATGATGAAATTAAGATACCTGCACTTAAAACC
TATTTGAACGAACAGGAAATTTCAGTAAGGAGGAATTCGAAATGA

Upstream 100 bases:

>100_bases
GTTCAACAACTTAAAACGATGAATCTTTATGGTGCAATTATCGGAAAAGCACTTTACGACGGTTCCATAAAACTACAAGA
TATATTGGAAGGTGAAATGG

Downstream 100 bases:

>100_bases
CCAAATCAATCTCCATTGAACATTTACAATTTGACGAGAAAGGATTAATACCAGCAATTGTGCAGGATCATCAATCTGGC
CAAGTATTAACCTTGGCTTA

Product: cyclase

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLAKRIIPCLDVDNGRVVKGKKFLDIQDVADPVELAKRYNDEGADELVFYDITASNEQRGIFLDVVEKVAKEIAIPFMVG
GGIRTTKDIHQVLRSGADKVSINSAAVQRPELIFESAQKFGSQCTVLSIDAKEIAVGKWNVFINGGRKDTGIDAIEWAKK
GESYGAGEIVVNAMDADGEKNGYNLPLTTAIATAVNIPVIASGGAGNIQHFKDVLSHEIDAALAASVFHYDEIKIPALKT
YLNEQEISVRRNSK

Sequences:

>Translated_254_residues
MLAKRIIPCLDVDNGRVVKGKKFLDIQDVADPVELAKRYNDEGADELVFYDITASNEQRGIFLDVVEKVAKEIAIPFMVG
GGIRTTKDIHQVLRSGADKVSINSAAVQRPELIFESAQKFGSQCTVLSIDAKEIAVGKWNVFINGGRKDTGIDAIEWAKK
GESYGAGEIVVNAMDADGEKNGYNLPLTTAIATAVNIPVIASGGAGNIQHFKDVLSHEIDAALAASVFHYDEIKIPALKT
YLNEQEISVRRNSK
>Mature_254_residues
MLAKRIIPCLDVDNGRVVKGKKFLDIQDVADPVELAKRYNDEGADELVFYDITASNEQRGIFLDVVEKVAKEIAIPFMVG
GGIRTTKDIHQVLRSGADKVSINSAAVQRPELIFESAQKFGSQCTVLSIDAKEIAVGKWNVFINGGRKDTGIDAIEWAKK
GESYGAGEIVVNAMDADGEKNGYNLPLTTAIATAVNIPVIASGGAGNIQHFKDVLSHEIDAALAASVFHYDEIKIPALKT
YLNEQEISVRRNSK

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=46.875, Blast_Score=217, Evalue=6e-58,
Organism=Escherichia coli, GI87082028, Length=207, Percent_Identity=24.6376811594203, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=33.1210191082803, Blast_Score=145, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_OCEIH (Q8ESS2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691468.1
- ProteinModelPortal:   Q8ESS2
- SMR:   Q8ESS2
- GeneID:   1015840
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0547
- NMPDR:   fig|221109.1.peg.550
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- BioCyc:   OIHE221109:OB0547-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27575; Mature: 27575

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVDNGRVVKGKKFLDIQDVADPVELAKRYNDEGADELVFYDITASNEQRG
CCHHHCCCEEECCCCEEECCCEEEEHHHHCCHHHHHHHCCCCCCCEEEEEEEECCCCCCC
IFLDVVEKVAKEIAIPFMVGGGIRTTKDIHQVLRSGADKVSINSAAVQRPELIFESAQKF
CHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCEEEECHHHHCCHHHHHHHHHHH
GSQCTVLSIDAKEIAVGKWNVFINGGRKDTGIDAIEWAKKGESYGAGEIVVNAMDADGEK
CCCEEEEEECHHHEEEEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCC
NGYNLPLTTAIATAVNIPVIASGGAGNIQHFKDVLSHEIDAALAASVFHYDEIKIPALKT
CCCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
YLNEQEISVRRNSK
HCCCHHCCEECCCC
>Mature Secondary Structure
MLAKRIIPCLDVDNGRVVKGKKFLDIQDVADPVELAKRYNDEGADELVFYDITASNEQRG
CCHHHCCCEEECCCCEEECCCEEEEHHHHCCHHHHHHHCCCCCCCEEEEEEEECCCCCCC
IFLDVVEKVAKEIAIPFMVGGGIRTTKDIHQVLRSGADKVSINSAAVQRPELIFESAQKF
CHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCEEEECHHHHCCHHHHHHHHHHH
GSQCTVLSIDAKEIAVGKWNVFINGGRKDTGIDAIEWAKKGESYGAGEIVVNAMDADGEK
CCCEEEEEECHHHEEEEEEEEEEECCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCC
NGYNLPLTTAIATAVNIPVIASGGAGNIQHFKDVLSHEIDAALAASVFHYDEIKIPALKT
CCCCCCHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
YLNEQEISVRRNSK
HCCCHHCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376