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The map label for this gene is hisA

Identifier: 23098003

GI number: 23098003

Start: 580769

End: 581491

Strand: Reverse

Name: hisA

Synonym: OB0548

Alternate gene names: 23098003

Gene position: 581491-580769 (Counterclockwise)

Preceding gene: 23098004

Following gene: 23098002

Centisome position: 16.02

GC content: 39.7

Gene sequence:

>723_bases
ATGATTATCTTTCCAGCAATTGATATACGAAACGGGAAATGCGTACGTTTAAGGCAAGGTGACTATAATCAAGAAACTAT
ATATAGCAACTCTCCTGTAGAAATGGCGAAAGAATGGGAAGCTTCCGGTGCTGAGTATTTACACACTGTTGACTTGGACG
GAGCAAAGTCAGGAGAATCGAATAACATTAACATTATTCAAGATGTTGCTCAAGCACTCTCTATTCCGATTCAAGTAGGA
GGCGGAATCCGGTCCCTCGAAGTCATTGATAAGTATATCCAAGCAGGTGTAAGCCGAGTGATTCTTGGAACAGCAGCAAT
AACAGATTCGGTCTTTTTACAAACCGCCGTTGAAAGTTACGCTGAGAAAATTGCAGTATCGATTGATGCGAGAAATGGTT
TTATTGCAACGGATGGCTGGACAAAGAATAGTACGGTAGAAGCAATCCCTTTTATCAAACAGTTAGAATCAATCGGAGTA
AAAACCATTATTTATACGGATATCTTAAAAGACGGCATGATGAGTGGCCCTAACTTCCAAGAACTAGATGCAGTTCAACA
AGCTACGACCATGAACATTATTGCTTCTGGGGGAGTTACTACAGAAAAAGATGTTCAACAACTTAAAACGATGAATCTTT
ATGGTGCAATTATCGGAAAAGCACTTTACGACGGTTCCATAAAACTACAAGATATATTGGAAGGTGAAATGGATGCTCGC
TAA

Upstream 100 bases:

>100_bases
CTATCGTTCAAAAAGGGAATATAACCGGTATGCAATTTCATCCAGAGAAGAGCGGCGAGTTTGGAATTGAATTACTAAAA
AGATATGAGGAGATGATTAG

Downstream 100 bases:

>100_bases
ACGAATTATTCCTTGCCTCGATGTTGATAACGGGCGAGTAGTTAAGGGAAAGAAATTCTTAGATATCCAAGATGTTGCTG
ATCCGGTTGAATTAGCAAAA

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MIIFPAIDIRNGKCVRLRQGDYNQETIYSNSPVEMAKEWEASGAEYLHTVDLDGAKSGESNNINIIQDVAQALSIPIQVG
GGIRSLEVIDKYIQAGVSRVILGTAAITDSVFLQTAVESYAEKIAVSIDARNGFIATDGWTKNSTVEAIPFIKQLESIGV
KTIIYTDILKDGMMSGPNFQELDAVQQATTMNIIASGGVTTEKDVQQLKTMNLYGAIIGKALYDGSIKLQDILEGEMDAR

Sequences:

>Translated_240_residues
MIIFPAIDIRNGKCVRLRQGDYNQETIYSNSPVEMAKEWEASGAEYLHTVDLDGAKSGESNNINIIQDVAQALSIPIQVG
GGIRSLEVIDKYIQAGVSRVILGTAAITDSVFLQTAVESYAEKIAVSIDARNGFIATDGWTKNSTVEAIPFIKQLESIGV
KTIIYTDILKDGMMSGPNFQELDAVQQATTMNIIASGGVTTEKDVQQLKTMNLYGAIIGKALYDGSIKLQDILEGEMDAR
>Mature_240_residues
MIIFPAIDIRNGKCVRLRQGDYNQETIYSNSPVEMAKEWEASGAEYLHTVDLDGAKSGESNNINIIQDVAQALSIPIQVG
GGIRSLEVIDKYIQAGVSRVILGTAAITDSVFLQTAVESYAEKIAVSIDARNGFIATDGWTKNSTVEAIPFIKQLESIGV
KTIIYTDILKDGMMSGPNFQELDAVQQATTMNIIASGGVTTEKDVQQLKTMNLYGAIIGKALYDGSIKLQDILEGEMDAR

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=33.0543933054393, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788336, Length=233, Percent_Identity=24.0343347639485, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_OCEIH (Q8ESS1)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691469.1
- ProteinModelPortal:   Q8ESS1
- SMR:   Q8ESS1
- GeneID:   1015843
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0548
- NMPDR:   fig|221109.1.peg.551
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BioCyc:   OIHE221109:OB0548-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26013; Mature: 26013

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIFPAIDIRNGKCVRLRQGDYNQETIYSNSPVEMAKEWEASGAEYLHTVDLDGAKSGES
CEEECEEEECCCCEEEEECCCCCCCEEECCCCHHHHHHHCCCCCCEEEEEECCCCCCCCC
NNINIIQDVAQALSIPIQVGGGIRSLEVIDKYIQAGVSRVILGTAAITDSVFLQTAVESY
CCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKIAVSIDARNGFIATDGWTKNSTVEAIPFIKQLESIGVKTIIYTDILKDGMMSGPNFQ
HHHEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHH
ELDAVQQATTMNIIASGGVTTEKDVQQLKTMNLYGAIIGKALYDGSIKLQDILEGEMDAR
HHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHCCCCCCC
>Mature Secondary Structure
MIIFPAIDIRNGKCVRLRQGDYNQETIYSNSPVEMAKEWEASGAEYLHTVDLDGAKSGES
CEEECEEEECCCCEEEEECCCCCCCEEECCCCHHHHHHHCCCCCCEEEEEECCCCCCCCC
NNINIIQDVAQALSIPIQVGGGIRSLEVIDKYIQAGVSRVILGTAAITDSVFLQTAVESY
CCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEKIAVSIDARNGFIATDGWTKNSTVEAIPFIKQLESIGVKTIIYTDILKDGMMSGPNFQ
HHHEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHH
ELDAVQQATTMNIIASGGVTTEKDVQQLKTMNLYGAIIGKALYDGSIKLQDILEGEMDAR
HHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376