Click here to switch to the map view.

The map label for this gene is hisI

Identifier: 23098001

GI number: 23098001

Start: 579371

End: 580018

Strand: Reverse

Name: hisI

Synonym: OB0546

Alternate gene names: 23098001

Gene position: 580018-579371 (Counterclockwise)

Preceding gene: 23098002

Following gene: 23098000

Centisome position: 15.98

GC content: 37.35

Gene sequence:

>648_bases
ATGACCAAATCAATCTCCATTGAACATTTACAATTTGACGAGAAAGGATTAATACCAGCAATTGTGCAGGATCATCAATC
TGGCCAAGTATTAACCTTGGCTTATATGAATAGCGATTCTATCACCAAAACAATCGAAACAAACGAAACCTGGTTTTACT
CTAGAAAAAGACAGGAGCTTTGGAATAAAGGGGCTACCTCAGGTAATAAGCAGACAGTAAAACAAATATCCTTTGATTGT
GATGCAGATGCGGTTCTTGTCCTTGTAGATGCACAAGGTCCAGCATGTCATACAGGAGAAGAAAGCTGCTTTTATGAAGA
TTTGTATAAAAATGATGTTGCGTTGCTGCAAATTATTCCGCAAGTGTCAGCTAAAATTAAGGAACGTCATGAACATCCAG
TTGAAGGTGCATACACCTCTTATTTATTTGAAAAAGGAGTAGATAAGATATTAAAGAAAATCGGGGAAGAAGCTACGGAA
GTTGTTATAGCTGCTAAAAACGAAGATAAGCAAGAATTAACAAGCGAGTTATCAGATCTTCTGTATCACTCACTAGTACT
CATGGAACAACAAGGAGTTACGTTAGAGGATATCAAAAAAGAGCTATATAAACGTCACGTCGAGAAAGAAGGCCAACAAC
GTGAGTAA

Upstream 100 bases:

>100_bases
CCGCCCTAGCAGCTTCTGTTTTTCACTATGATGAAATTAAGATACCTGCACTTAAAACCTATTTGAACGAACAGGAAATT
TCAGTAAGGAGGAATTCGAA

Downstream 100 bases:

>100_bases
ATTCTGGAGCTCCTTAGCTAAACAAGTAGATCCATACATTCCTGGAGAACAATTGAATGATGATACGATTATTAAACTAA
ATACAAATGAAAATCCGTAT

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTKSISIEHLQFDEKGLIPAIVQDHQSGQVLTLAYMNSDSITKTIETNETWFYSRKRQELWNKGATSGNKQTVKQISFDC
DADAVLVLVDAQGPACHTGEESCFYEDLYKNDVALLQIIPQVSAKIKERHEHPVEGAYTSYLFEKGVDKILKKIGEEATE
VVIAAKNEDKQELTSELSDLLYHSLVLMEQQGVTLEDIKKELYKRHVEKEGQQRE

Sequences:

>Translated_215_residues
MTKSISIEHLQFDEKGLIPAIVQDHQSGQVLTLAYMNSDSITKTIETNETWFYSRKRQELWNKGATSGNKQTVKQISFDC
DADAVLVLVDAQGPACHTGEESCFYEDLYKNDVALLQIIPQVSAKIKERHEHPVEGAYTSYLFEKGVDKILKKIGEEATE
VVIAAKNEDKQELTSELSDLLYHSLVLMEQQGVTLEDIKKELYKRHVEKEGQQRE
>Mature_214_residues
TKSISIEHLQFDEKGLIPAIVQDHQSGQVLTLAYMNSDSITKTIETNETWFYSRKRQELWNKGATSGNKQTVKQISFDCD
ADAVLVLVDAQGPACHTGEESCFYEDLYKNDVALLQIIPQVSAKIKERHEHPVEGAYTSYLFEKGVDKILKKIGEEATEV
VIAAKNEDKQELTSELSDLLYHSLVLMEQQGVTLEDIKKELYKRHVEKEGQQRE

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=191, Percent_Identity=42.9319371727749, Blast_Score=163, Evalue=7e-42,
Organism=Saccharomyces cerevisiae, GI6319819, Length=196, Percent_Identity=36.2244897959184, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS2_OCEIH (Q8CXM8)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691467.1
- ProteinModelPortal:   Q8CXM8
- GeneID:   1015838
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0546
- NMPDR:   fig|221109.1.peg.549
- HOGENOM:   HBG294308
- OMA:   VMACKDD
- ProtClustDB:   PRK02759
- BioCyc:   OIHE221109:OB0546-MONOMER
- BRENDA:   3.5.4.19
- BRENDA:   3.6.1.31
- GO:   GO:0005737
- HAMAP:   MF_01019
- InterPro:   IPR013785
- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD002610
- TIGRFAMs:   TIGR03188

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH

EC number: =3.5.4.19; =3.6.1.31

Molecular weight: Translated: 24449; Mature: 24318

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSISIEHLQFDEKGLIPAIVQDHQSGQVLTLAYMNSDSITKTIETNETWFYSRKRQEL
CCCCCEEHEEECCCCCCCHHHHCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHH
WNKGATSGNKQTVKQISFDCDADAVLVLVDAQGPACHTGEESCFYEDLYKNDVALLQIIP
HHCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
QVSAKIKERHEHPVEGAYTSYLFEKGVDKILKKIGEEATEVVIAAKNEDKQELTSELSDL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHH
LYHSLVLMEQQGVTLEDIKKELYKRHVEKEGQQRE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKSISIEHLQFDEKGLIPAIVQDHQSGQVLTLAYMNSDSITKTIETNETWFYSRKRQEL
CCCCEEHEEECCCCCCCHHHHCCCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHH
WNKGATSGNKQTVKQISFDCDADAVLVLVDAQGPACHTGEESCFYEDLYKNDVALLQIIP
HHCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
QVSAKIKERHEHPVEGAYTSYLFEKGVDKILKKIGEEATEVVIAAKNEDKQELTSELSDL
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHH
LYHSLVLMEQQGVTLEDIKKELYKRHVEKEGQQRE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376