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The map label for this gene is trpB

Identifier: 23097977

GI number: 23097977

Start: 554493

End: 555692

Strand: Reverse

Name: trpB

Synonym: OB0522

Alternate gene names: 23097977

Gene position: 555692-554493 (Counterclockwise)

Preceding gene: 23097978

Following gene: 23097976

Centisome position: 15.31

GC content: 40.5

Gene sequence:

>1200_bases
ATGACTACTTATTCATTTCCAAATACAAAAGGAAAATATGGAGAGTTTGGCGGAAGATTTGTTCCAGAACTATTAATGCC
TGCAGTACTTGAATTAGAAAAAGCGTATGAAGATGCGTTGAAAGATGAGAGTTTTAACGAGGAACTTCAAACATATTTAA
CGGAATACATCGGACGTGAAACCCCATTATATCACGCTAAAAAGTTAACGGAACATGCAGGCGGCGCACAAATTTATTTA
AAACGTGAAGATTTAAATCATACTGGTGCTCATAAAATCAATAATACAATTGGGCAAGCACTACTCACGTTGCGAATGGG
CAAGAAAAAAGTTGTAGCTGAAACTGGTGCAGGACAACATGGTGTAGCTACAGCAACAGTATGTTCTTTATTAGGATTAG
AATGTATTGTTTTTATGGGGGAAGAAGATATTAAGCGGCAAAAGCTAAATGTTTTTCGCATGGAATTGCTAGGGGCGGAG
GTAGTCAGTGTCTCCCAAGGTAGTGGAACCTTAAAAGACGCTGTGAACGAAGCGCTTCGCTACTGGGTAAATAATGTCAA
TGACACTCATTATATTATCGGATCAGTCGTTGGACCTCATCCTTTCCCAAAAATTGTTCGCGACTTTCAATCCGTGATAG
GCAAAGAAACCAAAGAACAAAGTATGAAAAAAATAGGTTCCTTACCCGATGCTGTTGTCGCATGCGTTGGTGGCGGAAGT
AATGCGATGGGCATGTTCTACCCATTTATCGATGATAAGGAAGTGACTTTATTCGGTGTAGAAGCTGCAGGAGCAGGATT
AGATACCAAACAACATGCAGCGACCCTTACTGGTGGCTCTCCAGGTATGTTGCATGGAACATTTACGTATCTGCTTCAAG
ATGATTGTGGACAAATTGAAGAAGCATTCTCTATCTCAGCAGGTCTTGATTATCCAGGAATTGGACCAGAACATAGTCAC
CTTCACCAGACGAAAAGAGTAACATACTCCTCGATTACAGATGAGGAAGCACTTGAAGCTTTTCAACTCTTATCCAAAAC
AGAAGGCATTATTCCTGCATTAGAGAGCGCACATGCAGTTAGTTATGCTACAAAGTTAGCTAAGGAAATGAATCCTGAGC
AATCTATCGTTATTTGCTTATCTGGTAGAGGGGATAAAGACGTCGAGCAAGTAAAAGAAAGACTGGAGGGGGAACAATAA

Upstream 100 bases:

>100_bases
CTGGTGTTGATGTTTCAAGTGGAGTAGAAACAAATGGAAAAAAGGACGCAAAGAAGATCCAGCAATTTATTACTAACGTA
CAAAGAAAGGATGTATTGCG

Downstream 100 bases:

>100_bases
TGGGAAAAAGTAAACTCGATGCTGTATTGAAAGAAAAAACACAAGCCAAAAAACCTATATTTGTACCTTATATTATGGCT
GGAGATGGTGGCATTGATAA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTTYSFPNTKGKYGEFGGRFVPELLMPAVLELEKAYEDALKDESFNEELQTYLTEYIGRETPLYHAKKLTEHAGGAQIYL
KREDLNHTGAHKINNTIGQALLTLRMGKKKVVAETGAGQHGVATATVCSLLGLECIVFMGEEDIKRQKLNVFRMELLGAE
VVSVSQGSGTLKDAVNEALRYWVNNVNDTHYIIGSVVGPHPFPKIVRDFQSVIGKETKEQSMKKIGSLPDAVVACVGGGS
NAMGMFYPFIDDKEVTLFGVEAAGAGLDTKQHAATLTGGSPGMLHGTFTYLLQDDCGQIEEAFSISAGLDYPGIGPEHSH
LHQTKRVTYSSITDEEALEAFQLLSKTEGIIPALESAHAVSYATKLAKEMNPEQSIVICLSGRGDKDVEQVKERLEGEQ

Sequences:

>Translated_399_residues
MTTYSFPNTKGKYGEFGGRFVPELLMPAVLELEKAYEDALKDESFNEELQTYLTEYIGRETPLYHAKKLTEHAGGAQIYL
KREDLNHTGAHKINNTIGQALLTLRMGKKKVVAETGAGQHGVATATVCSLLGLECIVFMGEEDIKRQKLNVFRMELLGAE
VVSVSQGSGTLKDAVNEALRYWVNNVNDTHYIIGSVVGPHPFPKIVRDFQSVIGKETKEQSMKKIGSLPDAVVACVGGGS
NAMGMFYPFIDDKEVTLFGVEAAGAGLDTKQHAATLTGGSPGMLHGTFTYLLQDDCGQIEEAFSISAGLDYPGIGPEHSH
LHQTKRVTYSSITDEEALEAFQLLSKTEGIIPALESAHAVSYATKLAKEMNPEQSIVICLSGRGDKDVEQVKERLEGEQ
>Mature_398_residues
TTYSFPNTKGKYGEFGGRFVPELLMPAVLELEKAYEDALKDESFNEELQTYLTEYIGRETPLYHAKKLTEHAGGAQIYLK
REDLNHTGAHKINNTIGQALLTLRMGKKKVVAETGAGQHGVATATVCSLLGLECIVFMGEEDIKRQKLNVFRMELLGAEV
VSVSQGSGTLKDAVNEALRYWVNNVNDTHYIIGSVVGPHPFPKIVRDFQSVIGKETKEQSMKKIGSLPDAVVACVGGGSN
AMGMFYPFIDDKEVTLFGVEAAGAGLDTKQHAATLTGGSPGMLHGTFTYLLQDDCGQIEEAFSISAGLDYPGIGPEHSHL
HQTKRVTYSSITDEEALEAFQLLSKTEGIIPALESAHAVSYATKLAKEMNPEQSIVICLSGRGDKDVEQVKERLEGEQ

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=398, Percent_Identity=53.0150753768844, Blast_Score=421, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6321412, Length=385, Percent_Identity=59.2207792207792, Blast_Score=482, Evalue=1e-137,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_OCEIH (Q8ESU4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691443.1
- ProteinModelPortal:   Q8ESU4
- SMR:   Q8ESU4
- GeneID:   1015792
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0522
- NMPDR:   fig|221109.1.peg.525
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   OIHE221109:OB0522-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43450; Mature: 43319

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTYSFPNTKGKYGEFGGRFVPELLMPAVLELEKAYEDALKDESFNEELQTYLTEYIGRE
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
TPLYHAKKLTEHAGGAQIYLKREDLNHTGAHKINNTIGQALLTLRMGKKKVVAETGAGQH
CCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCC
GVATATVCSLLGLECIVFMGEEDIKRQKLNVFRMELLGAEVVSVSQGSGTLKDAVNEALR
CHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
YWVNNVNDTHYIIGSVVGPHPFPKIVRDFQSVIGKETKEQSMKKIGSLPDAVVACVGGGS
HHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHEEEECCCC
NAMGMFYPFIDDKEVTLFGVEAAGAGLDTKQHAATLTGGSPGMLHGTFTYLLQDDCGQIE
CCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHHHH
EAFSISAGLDYPGIGPEHSHLHQTKRVTYSSITDEEALEAFQLLSKTEGIIPALESAHAV
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
SYATKLAKEMNPEQSIVICLSGRGDKDVEQVKERLEGEQ
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TTYSFPNTKGKYGEFGGRFVPELLMPAVLELEKAYEDALKDESFNEELQTYLTEYIGRE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
TPLYHAKKLTEHAGGAQIYLKREDLNHTGAHKINNTIGQALLTLRMGKKKVVAETGAGQH
CCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCC
GVATATVCSLLGLECIVFMGEEDIKRQKLNVFRMELLGAEVVSVSQGSGTLKDAVNEALR
CHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
YWVNNVNDTHYIIGSVVGPHPFPKIVRDFQSVIGKETKEQSMKKIGSLPDAVVACVGGGS
HHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHEEEECCCC
NAMGMFYPFIDDKEVTLFGVEAAGAGLDTKQHAATLTGGSPGMLHGTFTYLLQDDCGQIE
CCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHEEECCCCCCCCHHHHHHHHHHHHHHHH
EAFSISAGLDYPGIGPEHSHLHQTKRVTYSSITDEEALEAFQLLSKTEGIIPALESAHAV
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
SYATKLAKEMNPEQSIVICLSGRGDKDVEQVKERLEGEQ
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376