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The map label for this gene is trpA

Identifier: 23097976

GI number: 23097976

Start: 553705

End: 554493

Strand: Reverse

Name: trpA

Synonym: OB0521

Alternate gene names: 23097976

Gene position: 554493-553705 (Counterclockwise)

Preceding gene: 23097977

Following gene: 23097967

Centisome position: 15.27

GC content: 41.83

Gene sequence:

>789_bases
ATGGGAAAAAGTAAACTCGATGCTGTATTGAAAGAAAAAACACAAGCCAAAAAACCTATATTTGTACCTTATATTATGGC
TGGAGATGGTGGCATTGATAACCTTAACAAACGTATTCAGTTTTTAGAAGAAGCAGGTGCTTCGGCAGTGGAACTTGGAA
TTCCATTTTCTGATCCGGTAGCAGATGGGCCTGTCATTCAAGATGCAGGACAACGCGCGCTGGCAAATAAGACTACGATA
GGTTCTGTATTAGAAGCATTGGAAAGCGAAAAATCACAGCGAAACATCCCTGTTGTCCTAATGACCTATATTAACCCAGT
ATGGAAATATGGATTTGAACAATTTGCTAGAGATTGCTCTCAAGCAGGTGTCGACGGCATCATTATTCCGGATATTCCGA
TGGAAGAGGAGGACGATGTAGCATCTTCGTTAACGCAGCACGATATCGCGTTTATCCGTTTAGCTGCCATGACAAGTACA
GAAGACCGTCTGGAAAGAATAGCAAAACGATCAGAAGGATTTTTATATGCCGTATCTGTGACTGGTACTACAGGTGAACG
CGCTCAACACGAGAATGATGCGTTCCACTTTTTAGAAAAACTGAAGCAAATAAGCCATGTACCAGTATTAGCTGGATTTG
GTATATCCACTGCCGAACGCGCAAGAGAACTTTCTGCCGCTTGCGACGGCGTTGTTGTAGGAAGTAAAATTGTACAGTTA
TTTGAGCAAGGAGATGAAGATGGTATTCACTCTCTTATACGAGAGAGTATTGGAGAAAAAGTCATTTGA

Upstream 100 bases:

>100_bases
CTAAGGAAATGAATCCTGAGCAATCTATCGTTATTTGCTTATCTGGTAGAGGGGATAAAGACGTCGAGCAAGTAAAAGAA
AGACTGGAGGGGGAACAATA

Downstream 100 bases:

>100_bases
CCAATTTGTAAATATAAGAGGATGAGACAAAAAAGAGCCCACGTAACTGCGTGGACTCTTGATTTTTATCTTGTCATTGA
ACGAATATAGTCATATTGCA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MGKSKLDAVLKEKTQAKKPIFVPYIMAGDGGIDNLNKRIQFLEEAGASAVELGIPFSDPVADGPVIQDAGQRALANKTTI
GSVLEALESEKSQRNIPVVLMTYINPVWKYGFEQFARDCSQAGVDGIIIPDIPMEEEDDVASSLTQHDIAFIRLAAMTST
EDRLERIAKRSEGFLYAVSVTGTTGERAQHENDAFHFLEKLKQISHVPVLAGFGISTAERARELSAACDGVVVGSKIVQL
FEQGDEDGIHSLIRESIGEKVI

Sequences:

>Translated_262_residues
MGKSKLDAVLKEKTQAKKPIFVPYIMAGDGGIDNLNKRIQFLEEAGASAVELGIPFSDPVADGPVIQDAGQRALANKTTI
GSVLEALESEKSQRNIPVVLMTYINPVWKYGFEQFARDCSQAGVDGIIIPDIPMEEEDDVASSLTQHDIAFIRLAAMTST
EDRLERIAKRSEGFLYAVSVTGTTGERAQHENDAFHFLEKLKQISHVPVLAGFGISTAERARELSAACDGVVVGSKIVQL
FEQGDEDGIHSLIRESIGEKVI
>Mature_261_residues
GKSKLDAVLKEKTQAKKPIFVPYIMAGDGGIDNLNKRIQFLEEAGASAVELGIPFSDPVADGPVIQDAGQRALANKTTIG
SVLEALESEKSQRNIPVVLMTYINPVWKYGFEQFARDCSQAGVDGIIIPDIPMEEEDDVASSLTQHDIAFIRLAAMTSTE
DRLERIAKRSEGFLYAVSVTGTTGERAQHENDAFHFLEKLKQISHVPVLAGFGISTAERARELSAACDGVVVGSKIVQLF
EQGDEDGIHSLIRESIGEKVI

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=240, Percent_Identity=37.5, Blast_Score=149, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=33.4728033472803, Blast_Score=153, Evalue=3e-38,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_OCEIH (Q8ESU5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691442.1
- ProteinModelPortal:   Q8ESU5
- SMR:   Q8ESU5
- GeneID:   1015791
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0521
- NMPDR:   fig|221109.1.peg.524
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   OIHE221109:OB0521-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28459; Mature: 28327

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 51-51 ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSKLDAVLKEKTQAKKPIFVPYIMAGDGGIDNLNKRIQFLEEAGASAVELGIPFSDPV
CCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC
ADGPVIQDAGQRALANKTTIGSVLEALESEKSQRNIPVVLMTYINPVWKYGFEQFARDCS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHH
QAGVDGIIIPDIPMEEEDDVASSLTQHDIAFIRLAAMTSTEDRLERIAKRSEGFLYAVSV
HCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEE
TGTTGERAQHENDAFHFLEKLKQISHVPVLAGFGISTAERARELSAACDGVVVGSKIVQL
ECCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
FEQGDEDGIHSLIRESIGEKVI
HHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GKSKLDAVLKEKTQAKKPIFVPYIMAGDGGIDNLNKRIQFLEEAGASAVELGIPFSDPV
CHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC
ADGPVIQDAGQRALANKTTIGSVLEALESEKSQRNIPVVLMTYINPVWKYGFEQFARDCS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHH
QAGVDGIIIPDIPMEEEDDVASSLTQHDIAFIRLAAMTSTEDRLERIAKRSEGFLYAVSV
HCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEE
TGTTGERAQHENDAFHFLEKLKQISHVPVLAGFGISTAERARELSAACDGVVVGSKIVQL
ECCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
FEQGDEDGIHSLIRESIGEKVI
HHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376