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The map label for this gene is selB [H]

Identifier: 22127942

GI number: 22127942

Start: 4516344

End: 4518296

Strand: Reverse

Name: selB [H]

Synonym: y4073

Alternate gene names: 22127942

Gene position: 4518296-4516344 (Counterclockwise)

Preceding gene: 22127944

Following gene: 22127940

Centisome position: 98.21

GC content: 55.66

Gene sequence:

>1953_bases
ATGATTATTGTCACCGCAGGCCATGTCGATCATGGCAAAACCACACTTTTACAGGCCATTACCGGGGTTAATGCGGATCG
GTTGCCGGAAGAGAAGCAGCGGGGGATGACCATCGATCTGGGCTATGCCTACTGGCCACTTCCTGATGGCCGTATCATGG
GTTTTATTGACGTCCCCGGTCATGAGAAGTTTTTGGCGAACATGTTGGCGGGGGGGGGCGGTATTGATCACGCTCTGTTG
GTGGTGGCCTGTGATGATGGCGTGATGGCGCAGACCCGTGAACATCTGGCGATTTTGCGGCTGAGTGGTCGGCCAGCGCT
AACGGTGGCATTGACCAAAGCGGATCGGGTTGATGATGAGCGCATTGCACAGGTCCATCAGCAAATTTTGCAGGAGTTGG
TGGCACAAGGCTGGTCAGCGGAGCAGATCAGTCTGTTTGTCACGGCGGCCGTCACTGAGCGTGGTATCGGTGAGTTACGT
GAGCATCTGGCGCAATGTCATCAGCAACGTGACCCGAAGGGCCGATTACAGCGGCGTTTTCGTCTGGCCATTGACCGGGT
ATTCAGCGTAAAAGGGGCTGGCTTAGTGGTGACGGGGACCGCGTTGGCGGGCCAGGTGGCGGTAGGCGATACCCTGTGGT
TGACTGGCGGTGATTGCCCCGTGCGGGTGCGAGGTATTCATGCCCAGAACCAACCGGCACAGCAGGCGCAAGCCGGCCAG
CGCATTGCCCTGAATATCAGCGGTGATATCAGTAAGCAGCAAATCAACCGCGGTGATTGGTTATTGGCGCAGCCACCGTT
GCCACCGGTGGATCGGGTGCTGGTAACCGTTGATGCCGATACGCCGTTACAACACTGGCAGCCCTTACATCTGTATCATG
GGGCTCGCCATATTACGGGCCGTTTTTCACTGCTAGGTCACCAGCAGTTAATTGACGACCAACAACCGTTAATTGACCAT
CAACAAATCATAAATAATCAATCCACATCAGGCGGTCATTCCTCTCAGATTCTGGCCGAGTTGCTGCTGGACAGCCCTCT
GTGGCTGGTAGAAAACGACCGGGTGATCTTGCGTGATATCGGGGCGAAGAAAACCTTGGGTGGCGCACGGGTGATTCATC
TGGCGGTGCCATCACGTGGTAAACGGCAACCGGCGTATCTGGCTTGGTTAACCGCACTGGCCCAGGCGACGAGTGACCGA
GAGGTGTTGGATTTACATTTGGCGCAAGGGCCGGTCTCGGTGCGCACATTTAGTTGGGCGCGCCAGCTTACTGACAATGA
GATGGCGGCATTACTGGCGGCGACCGATACTGTCGTCGCCGGGGACATTGCGCTATCACATCCTCACGCACAGCAAGCGC
AACAGACCCTCTTGCATGTACTCGCCCTCTATCACCAACAGCATGGCGATCAGTTGGGGTTAGGGCGGGCGCGTTTGCGA
CGTATGGCTTTACCGACGCTGGACGAAGGGGTGGTATTCCGTCTGATTGATAACTTGCTGGCGGAGGGAGCGTTACACAA
CACCCGTGGGTGGTTACATCTGCCAGAACATGGTTTAGCGTTTACTGAGCAAGAGCAGATAGGGTGGCAACAAGCGGCAC
CTTACTTTGTTGATGACCCTTGGTGGGTGCGTGACTTAGCGGCCGAGTTGCAAGCTGCAGAGGGTGAAATGCGCAGCCTA
TTACGCAAAGCGGCGCAACTGGGTTATATCACTGCGGTTGTGACGGATCGTTATTATCTGAGCCAACGCATTGAGCAGCT
TGCTGATTTGGTACGTGAGTTAGATAGCCGCCAGGGGAGTGTCAGTGCCGCAGATTTCCGTGATCGACTCGGTATTGGTC
GCAAACTGGCGATCCAAATTCTTGAATTCTTTGACCGCAGTGGTTTCACCCGCCGCCGGGGTAATGGCCATATATTGCGA
GATAGCGGCCTGTTTTCTGCGACAGGTTCTTGA

Upstream 100 bases:

>100_bases
CACTGACGAAGCCGGTCATTGGTCGTGTGGCTGATGGGCGTTTGTGGCTGGATTTACGCTGTCTGGAGGATGAGGCGGCG
TTGCTAAGGGAATTGGCATC

Downstream 100 bases:

>100_bases
GGAGAGCTATATTGTTGCCAGATTAGCGTTTTTGTATAAATTGAACCCTACTTTGGCAGCAAAAGTATAAATATTTGAAG
GATTGATTATCGCATTTTTA

Product: selenocysteinyl-tRNA-specific translation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 650; Mature: 650

Protein sequence:

>650_residues
MIIVTAGHVDHGKTTLLQAITGVNADRLPEEKQRGMTIDLGYAYWPLPDGRIMGFIDVPGHEKFLANMLAGGGGIDHALL
VVACDDGVMAQTREHLAILRLSGRPALTVALTKADRVDDERIAQVHQQILQELVAQGWSAEQISLFVTAAVTERGIGELR
EHLAQCHQQRDPKGRLQRRFRLAIDRVFSVKGAGLVVTGTALAGQVAVGDTLWLTGGDCPVRVRGIHAQNQPAQQAQAGQ
RIALNISGDISKQQINRGDWLLAQPPLPPVDRVLVTVDADTPLQHWQPLHLYHGARHITGRFSLLGHQQLIDDQQPLIDH
QQIINNQSTSGGHSSQILAELLLDSPLWLVENDRVILRDIGAKKTLGGARVIHLAVPSRGKRQPAYLAWLTALAQATSDR
EVLDLHLAQGPVSVRTFSWARQLTDNEMAALLAATDTVVAGDIALSHPHAQQAQQTLLHVLALYHQQHGDQLGLGRARLR
RMALPTLDEGVVFRLIDNLLAEGALHNTRGWLHLPEHGLAFTEQEQIGWQQAAPYFVDDPWWVRDLAAELQAAEGEMRSL
LRKAAQLGYITAVVTDRYYLSQRIEQLADLVRELDSRQGSVSAADFRDRLGIGRKLAIQILEFFDRSGFTRRRGNGHILR
DSGLFSATGS

Sequences:

>Translated_650_residues
MIIVTAGHVDHGKTTLLQAITGVNADRLPEEKQRGMTIDLGYAYWPLPDGRIMGFIDVPGHEKFLANMLAGGGGIDHALL
VVACDDGVMAQTREHLAILRLSGRPALTVALTKADRVDDERIAQVHQQILQELVAQGWSAEQISLFVTAAVTERGIGELR
EHLAQCHQQRDPKGRLQRRFRLAIDRVFSVKGAGLVVTGTALAGQVAVGDTLWLTGGDCPVRVRGIHAQNQPAQQAQAGQ
RIALNISGDISKQQINRGDWLLAQPPLPPVDRVLVTVDADTPLQHWQPLHLYHGARHITGRFSLLGHQQLIDDQQPLIDH
QQIINNQSTSGGHSSQILAELLLDSPLWLVENDRVILRDIGAKKTLGGARVIHLAVPSRGKRQPAYLAWLTALAQATSDR
EVLDLHLAQGPVSVRTFSWARQLTDNEMAALLAATDTVVAGDIALSHPHAQQAQQTLLHVLALYHQQHGDQLGLGRARLR
RMALPTLDEGVVFRLIDNLLAEGALHNTRGWLHLPEHGLAFTEQEQIGWQQAAPYFVDDPWWVRDLAAELQAAEGEMRSL
LRKAAQLGYITAVVTDRYYLSQRIEQLADLVRELDSRQGSVSAADFRDRLGIGRKLAIQILEFFDRSGFTRRRGNGHILR
DSGLFSATGS
>Mature_650_residues
MIIVTAGHVDHGKTTLLQAITGVNADRLPEEKQRGMTIDLGYAYWPLPDGRIMGFIDVPGHEKFLANMLAGGGGIDHALL
VVACDDGVMAQTREHLAILRLSGRPALTVALTKADRVDDERIAQVHQQILQELVAQGWSAEQISLFVTAAVTERGIGELR
EHLAQCHQQRDPKGRLQRRFRLAIDRVFSVKGAGLVVTGTALAGQVAVGDTLWLTGGDCPVRVRGIHAQNQPAQQAQAGQ
RIALNISGDISKQQINRGDWLLAQPPLPPVDRVLVTVDADTPLQHWQPLHLYHGARHITGRFSLLGHQQLIDDQQPLIDH
QQIINNQSTSGGHSSQILAELLLDSPLWLVENDRVILRDIGAKKTLGGARVIHLAVPSRGKRQPAYLAWLTALAQATSDR
EVLDLHLAQGPVSVRTFSWARQLTDNEMAALLAATDTVVAGDIALSHPHAQQAQQTLLHVLALYHQQHGDQLGLGRARLR
RMALPTLDEGVVFRLIDNLLAEGALHNTRGWLHLPEHGLAFTEQEQIGWQQAAPYFVDDPWWVRDLAAELQAAEGEMRSL
LRKAAQLGYITAVVTDRYYLSQRIEQLADLVRELDSRQGSVSAADFRDRLGIGRKLAIQILEFFDRSGFTRRRGNGHILR
DSGLFSATGS

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=293, Percent_Identity=31.740614334471, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI54607086, Length=360, Percent_Identity=25.8333333333333, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI5729864, Length=316, Percent_Identity=27.8481012658228, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI223555963, Length=316, Percent_Identity=27.8481012658228, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI53729339, Length=234, Percent_Identity=29.0598290598291, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI53729337, Length=234, Percent_Identity=29.0598290598291, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI2367247, Length=645, Percent_Identity=59.3798449612403, Blast_Score=750, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=289, Percent_Identity=33.5640138408304, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789737, Length=289, Percent_Identity=33.5640138408304, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789108, Length=342, Percent_Identity=26.6081871345029, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=291, Percent_Identity=30.9278350515464, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25141371, Length=274, Percent_Identity=27.7372262773723, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17506081, Length=326, Percent_Identity=24.5398773006135, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115532067, Length=310, Percent_Identity=26.1290322580645, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115532065, Length=310, Percent_Identity=26.1290322580645, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32566629, Length=319, Percent_Identity=24.1379310344828, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994658, Length=234, Percent_Identity=28.2051282051282, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=290, Percent_Identity=31.3793103448276, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6325337, Length=388, Percent_Identity=22.4226804123711, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319594, Length=388, Percent_Identity=22.4226804123711, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324550, Length=145, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363316, Length=284, Percent_Identity=32.7464788732394, Blast_Score=130, Evalue=4e-30,
Organism=Drosophila melanogaster, GI17864358, Length=284, Percent_Identity=32.7464788732394, Blast_Score=130, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19921738, Length=282, Percent_Identity=32.6241134751773, Blast_Score=123, Evalue=5e-28,
Organism=Drosophila melanogaster, GI19922690, Length=366, Percent_Identity=27.0491803278689, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI45550900, Length=348, Percent_Identity=22.1264367816092, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28572034, Length=115, Percent_Identity=39.1304347826087, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 71587; Mature: 71587

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVTAGHVDHGKTTLLQAITGVNADRLPEEKQRGMTIDLGYAYWPLPDGRIMGFIDVPG
CEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEEECCCCCCEEEEEECCC
HEKFLANMLAGGGGIDHALLVVACDDGVMAQTREHLAILRLSGRPALTVALTKADRVDDE
HHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCEEEEEECCCCEEEEEEECCCCCCHH
RIAQVHQQILQELVAQGWSAEQISLFVTAAVTERGIGELREHLAQCHQQRDPKGRLQRRF
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHH
RLAIDRVFSVKGAGLVVTGTALAGQVAVGDTLWLTGGDCPVRVRGIHAQNQPAQQAQAGQ
HHHHHHHHHCCCCCEEEECCHHCCEEEECCEEEEECCCCCEEEEEECCCCCCHHHHHCCC
RIALNISGDISKQQINRGDWLLAQPPLPPVDRVLVTVDADTPLQHWQPLHLYHGARHITG
EEEEEECCCCCHHHCCCCCEEEECCCCCCCCEEEEEECCCCCHHHCCCCHHHCCCHHHCC
RFSLLGHQQLIDDQQPLIDHQQIINNQSTSGGHSSQILAELLLDSPLWLVENDRVILRDI
HHHHHCCHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCEEEEEEC
GAKKTLGGARVIHLAVPSRGKRQPAYLAWLTALAQATSDREVLDLHLAQGPVSVRTFSWA
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEHHHHH
RQLTDNEMAALLAATDTVVAGDIALSHPHAQQAQQTLLHVLALYHQQHGDQLGLGRARLR
HHCCCHHHHHHHHHCCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
RMALPTLDEGVVFRLIDNLLAEGALHNTRGWLHLPEHGLAFTEQEQIGWQQAAPYFVDDP
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCHHHCCHHHCCCCCCCCC
WWVRDLAAELQAAEGEMRSLLRKAAQLGYITAVVTDRYYLSQRIEQLADLVRELDSRQGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCC
VSAADFRDRLGIGRKLAIQILEFFDRSGFTRRRGNGHILRDSGLFSATGS
CCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEECCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MIIVTAGHVDHGKTTLLQAITGVNADRLPEEKQRGMTIDLGYAYWPLPDGRIMGFIDVPG
CEEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEEECCCCCCEEEEEECCC
HEKFLANMLAGGGGIDHALLVVACDDGVMAQTREHLAILRLSGRPALTVALTKADRVDDE
HHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHCEEEEEECCCCEEEEEEECCCCCCHH
RIAQVHQQILQELVAQGWSAEQISLFVTAAVTERGIGELREHLAQCHQQRDPKGRLQRRF
HHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHH
RLAIDRVFSVKGAGLVVTGTALAGQVAVGDTLWLTGGDCPVRVRGIHAQNQPAQQAQAGQ
HHHHHHHHHCCCCCEEEECCHHCCEEEECCEEEEECCCCCEEEEEECCCCCCHHHHHCCC
RIALNISGDISKQQINRGDWLLAQPPLPPVDRVLVTVDADTPLQHWQPLHLYHGARHITG
EEEEEECCCCCHHHCCCCCEEEECCCCCCCCEEEEEECCCCCHHHCCCCHHHCCCHHHCC
RFSLLGHQQLIDDQQPLIDHQQIINNQSTSGGHSSQILAELLLDSPLWLVENDRVILRDI
HHHHHCCHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCEEEEEEC
GAKKTLGGARVIHLAVPSRGKRQPAYLAWLTALAQATSDREVLDLHLAQGPVSVRTFSWA
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEHHHHH
RQLTDNEMAALLAATDTVVAGDIALSHPHAQQAQQTLLHVLALYHQQHGDQLGLGRARLR
HHCCCHHHHHHHHHCCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
RMALPTLDEGVVFRLIDNLLAEGALHNTRGWLHLPEHGLAFTEQEQIGWQQAAPYFVDDP
HHCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCHHHCCHHHCCCCCCCCC
WWVRDLAAELQAAEGEMRSLLRKAAQLGYITAVVTDRYYLSQRIEQLADLVRELDSRQGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCC
VSAADFRDRLGIGRKLAIQILEFFDRSGFTRRRGNGHILRDSGLFSATGS
CCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEECCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]