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Identifier: 21672742

GI number: 21672742

Start: 545791

End: 546294

Strand: Direct

Name: def

Synonym: BUsg477

Alternate gene names: 21672742

Gene position: 545791-546294 (Clockwise)

Preceding gene: 21672737

Following gene: 21672743

Centisome position: 85.09

GC content: 23.41

Gene sequence:

>504_bases
ATGTCTGTTTTAAAAATACTACATTATCCTGATCAAAGATTAAGACTAATTGCCAAACCAGTTGAAAAAATCACTAAAGA
AATATACAAAATTACAAATAATATGATAGATACTATGTATCAAGAAGAAGGAATTGGTTTAGCAGCAACACAAGTAAACA
TTCAACTGCAAATAATTGTGATTCATAAAATACACGCAATAGAAAAAAATTTGATTCTTATTAATCCAAAAATTATTGAA
AAAAAAGGCAGTATTAGTATTGAAGAAGGATGTTTATCAATTCCAGAATATCGTGCTTTTGTTCCACGTTTTAATTATAT
TAAAATTCAAGCTATAAATTTAAATGGAAATACTGTAGAAATAGAAGCAGATTCAATTCTTTCAATTTGTATACAACATG
AAATAGATCATCTAAATGGAAAACTATTTATAGATTATCTTTCTGAACTAAAGAGAGAAAGAATTTTAAAAAAATTAATA
AAATTAAAAAAAAGAATAAAATAA

Upstream 100 bases:

>100_bases
CTTTTTTTTTAAAATTTTAAAAAGAACTAAAAGTTAACTTTTAATTATAATAAAAAAGTTTATACTAAGATATAAATTAA
ATTAATTTACATCTAAAAAT

Downstream 100 bases:

>100_bases
AAATTTTTTAAACAAGTAGAAAAGAGAAAGTAAATTTGAAAAAATTAACAATTATTTTTGCTGGAACAGCATACTTCTCT
ATGAGATATTTAGATGCATT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSVLKILHYPDQRLRLIAKPVEKITKEIYKITNNMIDTMYQEEGIGLAATQVNIQLQIIVIHKIHAIEKNLILINPKIIE
KKGSISIEEGCLSIPEYRAFVPRFNYIKIQAINLNGNTVEIEADSILSICIQHEIDHLNGKLFIDYLSELKRERILKKLI
KLKKRIK

Sequences:

>Translated_167_residues
MSVLKILHYPDQRLRLIAKPVEKITKEIYKITNNMIDTMYQEEGIGLAATQVNIQLQIIVIHKIHAIEKNLILINPKIIE
KKGSISIEEGCLSIPEYRAFVPRFNYIKIQAINLNGNTVEIEADSILSICIQHEIDHLNGKLFIDYLSELKRERILKKLI
KLKKRIK
>Mature_166_residues
SVLKILHYPDQRLRLIAKPVEKITKEIYKITNNMIDTMYQEEGIGLAATQVNIQLQIIVIHKIHAIEKNLILINPKIIEK
KGSISIEEGCLSIPEYRAFVPRFNYIKIQAINLNGNTVEIEADSILSICIQHEIDHLNGKLFIDYLSELKRERILKKLIK
LKKRIK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=156, Percent_Identity=28.2051282051282, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=57.9268292682927, Blast_Score=202, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24645728, Length=171, Percent_Identity=28.0701754385965, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24645726, Length=140, Percent_Identity=29.2857142857143, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_BUCAP (Q8K975)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660809.1
- ProteinModelPortal:   Q8K975
- SMR:   Q8K975
- EnsemblBacteria:   EBBUCT00000000383
- GeneID:   1005692
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg477
- GeneTree:   EBGT00050000008071
- HOGENOM:   HBG665227
- OMA:   ETMIASE
- ProtClustDB:   PRK00150
- BioCyc:   BAPH198804:BUSG477-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19367; Mature: 19236

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLKILHYPDQRLRLIAKPVEKITKEIYKITNNMIDTMYQEEGIGLAATQVNIQLQIIV
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEE
IHKIHAIEKNLILINPKIIEKKGSISIEEGCLSIPEYRAFVPRFNYIKIQAINLNGNTVE
EEEEEHHCCCEEEECCEEECCCCCEEECCCCCCCCHHHHHCCCCCEEEEEEEECCCCEEE
IEADSILSICIQHEIDHLNGKLFIDYLSELKRERILKKLIKLKKRIK
EEHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVLKILHYPDQRLRLIAKPVEKITKEIYKITNNMIDTMYQEEGIGLAATQVNIQLQIIV
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEE
IHKIHAIEKNLILINPKIIEKKGSISIEEGCLSIPEYRAFVPRFNYIKIQAINLNGNTVE
EEEEEHHCCCEEEECCEEECCCCCEEECCCCCCCCHHHHHCCCCCEEEEEEEECCCCEEE
IEADSILSICIQHEIDHLNGKLFIDYLSELKRERILKKLIKLKKRIK
EEHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438