Click here to switch to the map view.

The map label for this gene is fmt

Identifier: 21672743

GI number: 21672743

Start: 546330

End: 547274

Strand: Direct

Name: fmt

Synonym: BUsg478

Alternate gene names: 21672743

Gene position: 546330-547274 (Clockwise)

Preceding gene: 21672742

Following gene: 21672781

Centisome position: 85.17

GC content: 26.03

Gene sequence:

>945_bases
TTGAAAAAATTAACAATTATTTTTGCTGGAACAGCATACTTCTCTATGAGATATTTAGATGCATTGACAAAATCTGAACA
CAAAGTAATAGCTGTAATTACTCAACCTGATCGTCCTTCTGGAAGAGGTCAAAAAATAATTTTTTCTCCTGTAAAAATAT
TATCTATAAAAAGAAATATTCCTATTTTTCAACCATCAGAATTAAAAAATGAAAAAATACAACGTGAAATATTCAATCTT
AATGCAGATATGATGATAGTAGTTTCTTATGGAAAACTCATACCAAAAGAAATACTAACTATGTTTCCAAAAGGTTGTAT
TAACGTTCATACTTCGTTATTACCTAGATGGAGAGGTGCTACTCCTATTCAATCTGCAATTTTATTTGGAGATAAAGAAA
CAGGTATAAGCATCATAAAAATGAATGAAAAAATGGATGCTGGAACTATAATTAATTCAGTTAAATGCAATATCTTACCT
AACGATACAACTGAAACATTAACATTTAAATTAATTGAAATCGGAATTCAAGTATTATTAAAAACTTTATATTATATAAA
TAAAAATATTGTTGTTGAAAAAGAACAAAATGAAAAACATGCAACTTTTTCAAAAAAAATATCTAAAAAAGATGCTTTAC
TCAATTGGAATACTGAAGCATATCTTTTAGAAAGATTAATACGTGCTTTTAATCCTTGGCCTGTCTGTTACTTTATTGTT
AACCAAATAGCAATAAGAGTATGGCAAGCAAAAATTATACCAACTGTTATGAAAAGTGCTTGTGTAGGAGAAATTGTCGC
TTTTAATAAAAATGGTATTCAAATAAATACAGCTCATCAAATATTAAATCTTCAAAAAATACAATTTCCTGGGAAAAAAA
TTATAAATGTAGAAAAAATAATTTCTTCTAAGAAACATTGGTTTCATATAGGAAAAATTATCTAA

Upstream 100 bases:

>100_bases
TTTCTGAACTAAAGAGAGAAAGAATTTTAAAAAAATTAATAAAATTAAAAAAAAGAATAAAATAAAAATTTTTTAAACAA
GTAGAAAAGAGAAAGTAAAT

Downstream 100 bases:

>100_bases
ATTAAATATATAATATTTATTATTTACTTTAATTTTTTAAAACATTAAAAAATGAGCAAACGGCGACCTTCCGTTTGCTT
TATATTATAAATAACTATTA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKLTIIFAGTAYFSMRYLDALTKSEHKVIAVITQPDRPSGRGQKIIFSPVKILSIKRNIPIFQPSELKNEKIQREIFNL
NADMMIVVSYGKLIPKEILTMFPKGCINVHTSLLPRWRGATPIQSAILFGDKETGISIIKMNEKMDAGTIINSVKCNILP
NDTTETLTFKLIEIGIQVLLKTLYYINKNIVVEKEQNEKHATFSKKISKKDALLNWNTEAYLLERLIRAFNPWPVCYFIV
NQIAIRVWQAKIIPTVMKSACVGEIVAFNKNGIQINTAHQILNLQKIQFPGKKIINVEKIISSKKHWFHIGKII

Sequences:

>Translated_314_residues
MKKLTIIFAGTAYFSMRYLDALTKSEHKVIAVITQPDRPSGRGQKIIFSPVKILSIKRNIPIFQPSELKNEKIQREIFNL
NADMMIVVSYGKLIPKEILTMFPKGCINVHTSLLPRWRGATPIQSAILFGDKETGISIIKMNEKMDAGTIINSVKCNILP
NDTTETLTFKLIEIGIQVLLKTLYYINKNIVVEKEQNEKHATFSKKISKKDALLNWNTEAYLLERLIRAFNPWPVCYFIV
NQIAIRVWQAKIIPTVMKSACVGEIVAFNKNGIQINTAHQILNLQKIQFPGKKIINVEKIISSKKHWFHIGKII
>Mature_314_residues
MKKLTIIFAGTAYFSMRYLDALTKSEHKVIAVITQPDRPSGRGQKIIFSPVKILSIKRNIPIFQPSELKNEKIQREIFNL
NADMMIVVSYGKLIPKEILTMFPKGCINVHTSLLPRWRGATPIQSAILFGDKETGISIIKMNEKMDAGTIINSVKCNILP
NDTTETLTFKLIEIGIQVLLKTLYYINKNIVVEKEQNEKHATFSKKISKKDALLNWNTEAYLLERLIRAFNPWPVCYFIV
NQIAIRVWQAKIIPTVMKSACVGEIVAFNKNGIQINTAHQILNLQKIQFPGKKIINVEKIISSKKHWFHIGKII

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=316, Percent_Identity=25, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI164663775, Length=235, Percent_Identity=28.0851063829787, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI21614513, Length=240, Percent_Identity=27.5, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789683, Length=310, Percent_Identity=44.1935483870968, Blast_Score=294, Evalue=5e-81,
Organism=Escherichia coli, GI1788589, Length=258, Percent_Identity=31.3953488372093, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI133930964, Length=281, Percent_Identity=24.5551601423488, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319458, Length=276, Percent_Identity=26.8115942028986, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI45550868, Length=230, Percent_Identity=31.304347826087, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28571984, Length=153, Percent_Identity=35.2941176470588, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_BUCAP (Q8K974)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660810.1
- ProteinModelPortal:   Q8K974
- SMR:   Q8K974
- EnsemblBacteria:   EBBUCT00000000298
- GeneID:   1005691
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg478
- GeneTree:   EBGT00050000008164
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   CLSK316292
- BioCyc:   BAPH198804:BUSG478-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 35831; Mature: 35831

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTIIFAGTAYFSMRYLDALTKSEHKVIAVITQPDRPSGRGQKIIFSPVKILSIKRNI
CCEEEEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEECCEEEEEEECCC
PIFQPSELKNEKIQREIFNLNADMMIVVSYGKLIPKEILTMFPKGCINVHTSLLPRWRGA
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHCCCCCCC
TPIQSAILFGDKETGISIIKMNEKMDAGTIINSVKCNILPNDTTETLTFKLIEIGIQVLL
CCHHHHEEECCCCCCEEEEEECCCCCCCCEEECEEEEECCCCCCHHHHHHHHHHHHHHHH
KTLYYINKNIVVEKEQNEKHATFSKKISKKDALLNWNTEAYLLERLIRAFNPWPVCYFIV
HHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHH
NQIAIRVWQAKIIPTVMKSACVGEIVAFNKNGIQINTAHQILNLQKIQFPGKKIINVEKI
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHHHHEECCCCCHHEEHHHH
ISSKKHWFHIGKII
HCCCCCEEEECCCC
>Mature Secondary Structure
MKKLTIIFAGTAYFSMRYLDALTKSEHKVIAVITQPDRPSGRGQKIIFSPVKILSIKRNI
CCEEEEEEECHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEECCEEEEEEECCC
PIFQPSELKNEKIQREIFNLNADMMIVVSYGKLIPKEILTMFPKGCINVHTSLLPRWRGA
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHCCCCCCC
TPIQSAILFGDKETGISIIKMNEKMDAGTIINSVKCNILPNDTTETLTFKLIEIGIQVLL
CCHHHHEEECCCCCCEEEEEECCCCCCCCEEECEEEEECCCCCCHHHHHHHHHHHHHHHH
KTLYYINKNIVVEKEQNEKHATFSKKISKKDALLNWNTEAYLLERLIRAFNPWPVCYFIV
HHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHH
NQIAIRVWQAKIIPTVMKSACVGEIVAFNKNGIQINTAHQILNLQKIQFPGKKIINVEKI
HHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHHHHEECCCCCHHEEHHHH
ISSKKHWFHIGKII
HCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438