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The map label for this gene is argA

Identifier: 21672706

GI number: 21672706

Start: 503863

End: 505191

Strand: Reverse

Name: argA

Synonym: BUsg441

Alternate gene names: 21672706

Gene position: 505191-503863 (Counterclockwise)

Preceding gene: 21672713

Following gene: 21672698

Centisome position: 78.76

GC content: 30.02

Gene sequence:

>1329_bases
ATGAGAGAGCGTACTACTGAATTAGTTCAGGGTTTTCGCCACAGTGTACCCTATATTAATGCTCATCGAGGTAAAAAATT
TGTAATAATGCTAAGTGGTGAAGCCATTAAATATGGAAATTTTTCTGGTATTATTAATGATATAGGATTACTGCATAGTT
TAGGTATTCGTTTAATTGTAGTGTATGGATCTTGTCCTCAAATTAATGCTAATTTAAAGGAAAAAAATATTAAAATAACT
TATCATAAGTATATACGCGTCACTGACTTATTATCTTTAGAACAAGTAAAACAAGCAGCAGGAAGATTACAGCTTGACAT
AACAGCACGTCTTTCTATGAGTTTAAGTAACACCCCCTTACAAGGAGCTAATATTAATGTTGTTAGCGGTAATTTTATTA
TTGCTCAGCCTTTAGGTGTAGACGATGGTATAGATTATTGTCACAGCGGTCGTATTAGAAGAATTGATAAAAAAGCTATT
AATTGTCAATTAGAAAATGGAGCCATAGTTTTAATTGGACCTGTAGCTGTTTCTGTTACAGGAGAAAGTTTTAATTTAAC
TTCTGAAGAAATTGCAACTCAAGTTAGTATTAAGTTAAAAGCAGAAAAAATGATAGGTTTTTGCAGTAAACAAGGTGTAA
TTGATAGTCAAGGAAAAACTATTTCTGAATTACTTCCTAATAATATACAAAATGAAATTAAAAAATTAGAAGGGAAAGGT
GATTATATTTCTTCAACAATACGATTTTTAAGAGGATCAATTAAAGCTTGTAAAAGCGGAGTCAATCGTAGTCATTTAAT
TAGCTATCATAAAAATGGAGCATTACTACAAGAATTATTTTCGCGTGATGGAATTGGAACGCAGATGGTTATGGAATCTG
CAGAAAAAATCAGACAAGCAAATATTAATGATATTGGAGGTATTTTAGAATTAATTCGTCCTTTAGAAAAAAAAGGTATT
TTAGTACGCAGATCTAGAGAACAATTAGAGATGGAAGTAGATAAATTCACTATTATTGAACGTGATAATTTAACTATTGC
TTGTGCTGCATTATACCCTTTTTTTAAGGAAAAAATAGGTGAAATGGCATGTGTAGCTGTTCATCCTGATTACCGAAATT
CTTCAAGGGGAGATTTATTATTGCAAACAATGAAGTTAAATGCTAAAAAGCTAAATTTAAAAAAAATTTTTGTCCTTACT
ACTCAAAGTATTCATTGGTTTCAAGAAAGAGGCTTCATTTTAGTAGATGTTGAAATATTGCCTGAAAGTAAAAAGAAAAT
GTATAATTATCAACGTTGTTCGAAAATTTTAATGATTGATTTATTTTAG

Upstream 100 bases:

>100_bases
AATTTTTTTGTTTTTTTTAAGATAAATTGTGTATTTTTAAAAAAAGTAAATTATATAAAAAATAATATATATATTTGAAT
AAAAATGCAAGAGGATTGTC

Downstream 100 bases:

>100_bases
ATGATTACTTTTAAAGTATCTTAATTTTTTAAGACTGTTTTAATAAATATTTCTTTTGTTGAAAAAATATTTAATTGTTT
CCGAGTTCTTGTAATTCCAG

Product: N-acetylglutamate synthase

Products: NA

Alternate protein names: N-acetylglutamate synthase; AGS; NAGS

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MRERTTELVQGFRHSVPYINAHRGKKFVIMLSGEAIKYGNFSGIINDIGLLHSLGIRLIVVYGSCPQINANLKEKNIKIT
YHKYIRVTDLLSLEQVKQAAGRLQLDITARLSMSLSNTPLQGANINVVSGNFIIAQPLGVDDGIDYCHSGRIRRIDKKAI
NCQLENGAIVLIGPVAVSVTGESFNLTSEEIATQVSIKLKAEKMIGFCSKQGVIDSQGKTISELLPNNIQNEIKKLEGKG
DYISSTIRFLRGSIKACKSGVNRSHLISYHKNGALLQELFSRDGIGTQMVMESAEKIRQANINDIGGILELIRPLEKKGI
LVRRSREQLEMEVDKFTIIERDNLTIACAALYPFFKEKIGEMACVAVHPDYRNSSRGDLLLQTMKLNAKKLNLKKIFVLT
TQSIHWFQERGFILVDVEILPESKKKMYNYQRCSKILMIDLF

Sequences:

>Translated_442_residues
MRERTTELVQGFRHSVPYINAHRGKKFVIMLSGEAIKYGNFSGIINDIGLLHSLGIRLIVVYGSCPQINANLKEKNIKIT
YHKYIRVTDLLSLEQVKQAAGRLQLDITARLSMSLSNTPLQGANINVVSGNFIIAQPLGVDDGIDYCHSGRIRRIDKKAI
NCQLENGAIVLIGPVAVSVTGESFNLTSEEIATQVSIKLKAEKMIGFCSKQGVIDSQGKTISELLPNNIQNEIKKLEGKG
DYISSTIRFLRGSIKACKSGVNRSHLISYHKNGALLQELFSRDGIGTQMVMESAEKIRQANINDIGGILELIRPLEKKGI
LVRRSREQLEMEVDKFTIIERDNLTIACAALYPFFKEKIGEMACVAVHPDYRNSSRGDLLLQTMKLNAKKLNLKKIFVLT
TQSIHWFQERGFILVDVEILPESKKKMYNYQRCSKILMIDLF
>Mature_442_residues
MRERTTELVQGFRHSVPYINAHRGKKFVIMLSGEAIKYGNFSGIINDIGLLHSLGIRLIVVYGSCPQINANLKEKNIKIT
YHKYIRVTDLLSLEQVKQAAGRLQLDITARLSMSLSNTPLQGANINVVSGNFIIAQPLGVDDGIDYCHSGRIRRIDKKAI
NCQLENGAIVLIGPVAVSVTGESFNLTSEEIATQVSIKLKAEKMIGFCSKQGVIDSQGKTISELLPNNIQNEIKKLEGKG
DYISSTIRFLRGSIKACKSGVNRSHLISYHKNGALLQELFSRDGIGTQMVMESAEKIRQANINDIGGILELIRPLEKKGI
LVRRSREQLEMEVDKFTIIERDNLTIACAALYPFFKEKIGEMACVAVHPDYRNSSRGDLLLQTMKLNAKKLNLKKIFVLT
TQSIHWFQERGFILVDVEILPESKKKMYNYQRCSKILMIDLF

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain

Homologues:

Organism=Escherichia coli, GI1789181, Length=441, Percent_Identity=75.7369614512472, Blast_Score=701, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320913, Length=302, Percent_Identity=21.8543046357616, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ARGA_BUCAP (P59099)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660773.1
- ProteinModelPortal:   P59099
- SMR:   P59099
- EnsemblBacteria:   EBBUCT00000000309
- GeneID:   1005723
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg441
- GeneTree:   EBGT00050000007812
- HOGENOM:   HBG545264
- OMA:   APYIHAF
- ProtClustDB:   PRK05279
- BioCyc:   BAPH198804:BUSG441-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01105
- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR001048
- InterPro:   IPR010167
- Gene3D:   G3DSA:3.40.1160.10
- Gene3D:   G3DSA:3.40.630.30
- PIRSF:   PIRSF000423
- TIGRFAMs:   TIGR01890

Pfam domain/function: PF00696 AA_kinase; PF00583 Acetyltransf_1; SSF53633 Aa_kinase; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.1.1

Molecular weight: Translated: 49586; Mature: 49586

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS51186 GNAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERTTELVQGFRHSVPYINAHRGKKFVIMLSGEAIKYGNFSGIINDIGLLHSLGIRLIV
CCCHHHHHHHHHHHCCCEEECCCCCEEEEEEECCEEEECCCHHHHHHHHHHHHCCEEEEE
VYGSCPQINANLKEKNIKITYHKYIRVTDLLSLEQVKQAAGRLQLDITARLSMSLSNTPL
EECCCCCCCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCC
QGANINVVSGNFIIAQPLGVDDGIDYCHSGRIRRIDKKAINCQLENGAIVLIGPVAVSVT
CCCEEEEEECCEEEEECCCCCCCCHHHCCCCEEEECCCEEEEEECCCEEEEEEEEEEEEE
GESFNLTSEEIATQVSIKLKAEKMIGFCSKQGVIDSQGKTISELLPNNIQNEIKKLEGKG
CCCCCCCHHHHHEEEEEEEEHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCC
DYISSTIRFLRGSIKACKSGVNRSHLISYHKNGALLQELFSRDGIGTQMVMESAEKIRQA
HHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHC
NINDIGGILELIRPLEKKGILVRRSREQLEMEVDKFTIIERDNLTIACAALYPFFKEKIG
CCHHHHHHHHHHHHHHHCCEEEEECHHHHHEECCEEEEEECCCCEEEHHHHHHHHHHHHC
EMACVAVHPDYRNSSRGDLLLQTMKLNAKKLNLKKIFVLTTQSIHWFQERGFILVDVEIL
CEEEEEECCCCCCCCCCCEEEEEECCCCEEECEEEEEEEEECCHHHHHHCCEEEEEEEEC
PESKKKMYNYQRCSKILMIDLF
CCHHHHHHCHHHHCEEEEEEEC
>Mature Secondary Structure
MRERTTELVQGFRHSVPYINAHRGKKFVIMLSGEAIKYGNFSGIINDIGLLHSLGIRLIV
CCCHHHHHHHHHHHCCCEEECCCCCEEEEEEECCEEEECCCHHHHHHHHHHHHCCEEEEE
VYGSCPQINANLKEKNIKITYHKYIRVTDLLSLEQVKQAAGRLQLDITARLSMSLSNTPL
EECCCCCCCCCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCC
QGANINVVSGNFIIAQPLGVDDGIDYCHSGRIRRIDKKAINCQLENGAIVLIGPVAVSVT
CCCEEEEEECCEEEEECCCCCCCCHHHCCCCEEEECCCEEEEEECCCEEEEEEEEEEEEE
GESFNLTSEEIATQVSIKLKAEKMIGFCSKQGVIDSQGKTISELLPNNIQNEIKKLEGKG
CCCCCCCHHHHHEEEEEEEEHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCC
DYISSTIRFLRGSIKACKSGVNRSHLISYHKNGALLQELFSRDGIGTQMVMESAEKIRQA
HHHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHC
NINDIGGILELIRPLEKKGILVRRSREQLEMEVDKFTIIERDNLTIACAALYPFFKEKIG
CCHHHHHHHHHHHHHHHCCEEEEECHHHHHEECCEEEEEECCCCEEEHHHHHHHHHHHHC
EMACVAVHPDYRNSSRGDLLLQTMKLNAKKLNLKKIFVLTTQSIHWFQERGFILVDVEIL
CEEEEEECCCCCCCCCCCEEEEEECCCCEEECEEEEEEEEECCHHHHHHCCEEEEEEEEC
PESKKKMYNYQRCSKILMIDLF
CCHHHHHHCHHHHCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438