Click here to switch to the map view.

The map label for this gene is asnC

Identifier: 21672623

GI number: 21672623

Start: 393231

End: 394631

Strand: Reverse

Name: asnC

Synonym: BUsg348

Alternate gene names: 21672623

Gene position: 394631-393231 (Counterclockwise)

Preceding gene: 21672624

Following gene: 21672622

Centisome position: 61.52

GC content: 28.12

Gene sequence:

>1401_bases
ATGAGTAGAATATCAATATCAGAAATTTATAAAGATGATATTATAGTAAATACCCCTATCACTATATTTGGATGGGTTCG
AAGTCGTAGAAGTTCAAAATCTGGTTTTTCTTTTATTACGGTTTATGACGGTTCGTGTTTGAATTCTGTACAAGTTGTTG
CCGATAAAACCTTATCTAATTATTACAAAGATATATTGCATTTAACTATTGGATGTTCTGTAACAATAACTGGAATTCTT
ATTTTATCTATTGGAGATAAACAAAAATATGAAATAAAAGCAACAAAGTTTCAAGTGTTAGGATGGATTAAAAATCCAGA
TACTTATCCAATATCTGCTAAAAAGCATAGTCTAGAATACCTAAGAGAAGTAGCACATTTACGTTCTAGAACTAATTTGA
TTGGCGTAATAGTAAGAATAAGGCATCATATATTTCAATCATTACATAAATTTTTAAATAAACAAGGCTATTATTGGATT
CCTACACCGATTATTACTGGACTTAATACAGAAGGTACAGGAGAGATGTTTCGTGTTTCAACGATGGATATGAGAAACAT
TCCTAAAAAAATAAATGGTTCCGTTGATTTTAAAAAAGATTTTTTTGGAAAAGAATCTTTTTTAACTGTTTCGGGACAAC
TTAATTTAGAAGCATATGCTTGTTCTTTATCAAAAGTATATACGTTTGGTCCTACATTTCGAGCTGAAAATTCTAATACT
AGTCGTCATTTAGCAGAATTTTGGATGTTAGAAATTGAATCTTCGTTTTGTAATTTAGATGAAATATTAATATTTTCTGA
AGATATGTTGAAATATATTTGTAAATCTCTCTTAAAATATTGTATTAACGACATTAAATTTCTTAAAAATTACATTGATA
ATGATATAATTAATCGTCTTAAAAAATTTTTGTCAGTAAACTTTATACGTATAAATTATAAAGATGCTATAGATATTTTA
TTAAATTCTAAAAAAAAATTTGATAATGTTGTTTCTTTCGGAGTTGATCTTAACGCTGAGCATGAGCGATTTCTTGTAGA
AAAGCATTTTAAAGCGCCTGTAGTAATAATAAATTATCCTAAAGAACTAAAAGCGTTTTATATGAGATTAAATGATGATA
AAAAAACTGTTGCTGCAATGGATTTATTAGTTCCAGGTATTGGAGAATTAATAGGTGGATCTCAACGTGAAGAACGTATT
TCTGTTTTAGATTTACGTTTATCAGAATTAGGTTTAAGAAAAGAAGATTATTGGTGGTATCGTGATCTTCGTCGTTATGG
TACGGTCCATCATTCAGGTTTTGGAATGGGTTTTGAGCGGTTAATATCTTATATTACTGGAATATCGAATATAAGAGATA
TTATTCCATTCCCTCGTACTGTTAAAAATGCTGATTTTTAA

Upstream 100 bases:

>100_bases
ATGTAAAGCATTTGATTTGTCTTTGACAGTGTAATTTAATACTAGTCACTTTGATTTGCTATTTTTCGATAGACTATTTA
AAAATATACAAGGCAAAATT

Downstream 100 bases:

>100_bases
TAAATTTTTTTTATATAAAATTATTTGTTATTTTAATAATTTTTTATTAATTAAAATTTTATGAAAATATGATAATTATT
TAATTTCAATTTATTTTAAT

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSRISISEIYKDDIIVNTPITIFGWVRSRRSSKSGFSFITVYDGSCLNSVQVVADKTLSNYYKDILHLTIGCSVTITGIL
ILSIGDKQKYEIKATKFQVLGWIKNPDTYPISAKKHSLEYLREVAHLRSRTNLIGVIVRIRHHIFQSLHKFLNKQGYYWI
PTPIITGLNTEGTGEMFRVSTMDMRNIPKKINGSVDFKKDFFGKESFLTVSGQLNLEAYACSLSKVYTFGPTFRAENSNT
SRHLAEFWMLEIESSFCNLDEILIFSEDMLKYICKSLLKYCINDIKFLKNYIDNDIINRLKKFLSVNFIRINYKDAIDIL
LNSKKKFDNVVSFGVDLNAEHERFLVEKHFKAPVVIINYPKELKAFYMRLNDDKKTVAAMDLLVPGIGELIGGSQREERI
SVLDLRLSELGLRKEDYWWYRDLRRYGTVHHSGFGMGFERLISYITGISNIRDIIPFPRTVKNADF

Sequences:

>Translated_466_residues
MSRISISEIYKDDIIVNTPITIFGWVRSRRSSKSGFSFITVYDGSCLNSVQVVADKTLSNYYKDILHLTIGCSVTITGIL
ILSIGDKQKYEIKATKFQVLGWIKNPDTYPISAKKHSLEYLREVAHLRSRTNLIGVIVRIRHHIFQSLHKFLNKQGYYWI
PTPIITGLNTEGTGEMFRVSTMDMRNIPKKINGSVDFKKDFFGKESFLTVSGQLNLEAYACSLSKVYTFGPTFRAENSNT
SRHLAEFWMLEIESSFCNLDEILIFSEDMLKYICKSLLKYCINDIKFLKNYIDNDIINRLKKFLSVNFIRINYKDAIDIL
LNSKKKFDNVVSFGVDLNAEHERFLVEKHFKAPVVIINYPKELKAFYMRLNDDKKTVAAMDLLVPGIGELIGGSQREERI
SVLDLRLSELGLRKEDYWWYRDLRRYGTVHHSGFGMGFERLISYITGISNIRDIIPFPRTVKNADF
>Mature_465_residues
SRISISEIYKDDIIVNTPITIFGWVRSRRSSKSGFSFITVYDGSCLNSVQVVADKTLSNYYKDILHLTIGCSVTITGILI
LSIGDKQKYEIKATKFQVLGWIKNPDTYPISAKKHSLEYLREVAHLRSRTNLIGVIVRIRHHIFQSLHKFLNKQGYYWIP
TPIITGLNTEGTGEMFRVSTMDMRNIPKKINGSVDFKKDFFGKESFLTVSGQLNLEAYACSLSKVYTFGPTFRAENSNTS
RHLAEFWMLEIESSFCNLDEILIFSEDMLKYICKSLLKYCINDIKFLKNYIDNDIINRLKKFLSVNFIRINYKDAIDILL
NSKKKFDNVVSFGVDLNAEHERFLVEKHFKAPVVIINYPKELKAFYMRLNDDKKTVAAMDLLVPGIGELIGGSQREERIS
VLDLRLSELGLRKEDYWWYRDLRRYGTVHHSGFGMGFERLISYITGISNIRDIIPFPRTVKNADF

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI226491502, Length=444, Percent_Identity=45.2702702702703, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI4758762, Length=451, Percent_Identity=27.9379157427938, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI45439306, Length=340, Percent_Identity=27.3529411764706, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1787161, Length=466, Percent_Identity=61.3733905579399, Blast_Score=660, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17556372, Length=426, Percent_Identity=31.924882629108, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71984122, Length=450, Percent_Identity=28.6666666666667, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17551876, Length=342, Percent_Identity=28.3625730994152, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319872, Length=488, Percent_Identity=35.2459016393443, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6321807, Length=468, Percent_Identity=27.3504273504274, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6323011, Length=348, Percent_Identity=27.5862068965517, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922416, Length=444, Percent_Identity=36.4864864864865, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI19921528, Length=462, Percent_Identity=28.5714285714286, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17136276, Length=340, Percent_Identity=27.0588235294118, Blast_Score=95, Evalue=9e-20,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYN_BUCAP (Q8K9I7)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660690.1
- ProteinModelPortal:   Q8K9I7
- SMR:   Q8K9I7
- EnsemblBacteria:   EBBUCT00000000519
- GeneID:   1005808
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg348
- GeneTree:   EBGT00050000007970
- HOGENOM:   HBG745843
- OMA:   EPEMAFC
- ProtClustDB:   PRK03932
- BioCyc:   BAPH198804:BUSG348-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00534
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00457

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.22

Molecular weight: Translated: 53869; Mature: 53738

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISISEIYKDDIIVNTPITIFGWVRSRRSSKSGFSFITVYDGSCLNSVQVVADKTLSN
CCCCCHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
YYKDILHLTIGCSVTITGILILSIGDKQKYEIKATKFQVLGWIKNPDTYPISAKKHSLEY
HHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHH
LREVAHLRSRTNLIGVIVRIRHHIFQSLHKFLNKQGYYWIPTPIITGLNTEGTGEMFRVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHEECCCCCCCCCEEEEE
TMDMRNIPKKINGSVDFKKDFFGKESFLTVSGQLNLEAYACSLSKVYTFGPTFRAENSNT
ECCHHCCCHHHCCCCCCHHHHCCCCCEEEEECEEEEEEEEEEHHHEEECCCCEECCCCCC
SRHLAEFWMLEIESSFCNLDEILIFSEDMLKYICKSLLKYCINDIKFLKNYIDNDIINRL
HHHHHHHHHHHHHHHHCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
KKFLSVNFIRINYKDAIDILLNSKKKFDNVVSFGVDLNAEHERFLVEKHFKAPVVIINYP
HHHHCEEEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEECC
KELKAFYMRLNDDKKTVAAMDLLVPGIGELIGGSQREERISVLDLRLSELGLRKEDYWWY
HHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
RDLRRYGTVHHSGFGMGFERLISYITGISNIRDIIPFPRTVKNADF
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SRISISEIYKDDIIVNTPITIFGWVRSRRSSKSGFSFITVYDGSCLNSVQVVADKTLSN
CCCCHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
YYKDILHLTIGCSVTITGILILSIGDKQKYEIKATKFQVLGWIKNPDTYPISAKKHSLEY
HHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEEEEEEEEEECCCCCCCCCCHHHHHHH
LREVAHLRSRTNLIGVIVRIRHHIFQSLHKFLNKQGYYWIPTPIITGLNTEGTGEMFRVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHEECCCCCCCCCEEEEE
TMDMRNIPKKINGSVDFKKDFFGKESFLTVSGQLNLEAYACSLSKVYTFGPTFRAENSNT
ECCHHCCCHHHCCCCCCHHHHCCCCCEEEEECEEEEEEEEEEHHHEEECCCCEECCCCCC
SRHLAEFWMLEIESSFCNLDEILIFSEDMLKYICKSLLKYCINDIKFLKNYIDNDIINRL
HHHHHHHHHHHHHHHHCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
KKFLSVNFIRINYKDAIDILLNSKKKFDNVVSFGVDLNAEHERFLVEKHFKAPVVIINYP
HHHHCEEEEEEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEECC
KELKAFYMRLNDDKKTVAAMDLLVPGIGELIGGSQREERISVLDLRLSELGLRKEDYWWY
HHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
RDLRRYGTVHHSGFGMGFERLISYITGISNIRDIIPFPRTVKNADF
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438