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The map label for this gene is pncB

Identifier: 21672624

GI number: 21672624

Start: 394698

End: 395894

Strand: Reverse

Name: pncB

Synonym: BUsg349

Alternate gene names: 21672624

Gene position: 395894-394698 (Counterclockwise)

Preceding gene: 21672628

Following gene: 21672623

Centisome position: 61.72

GC content: 25.73

Gene sequence:

>1197_bases
ATGAAACAATATAATTATCCAATAGTGAAAACATTACTTGATACTGATGCATATAAATTTTATATGCAGCAAGCTGTTTT
TTATCATTATAAAAATGTCGATGTAGTTGCAGAATTTATTTGCAGAGGTCCTAATATTTTAGGTTGTTATTCTCATATTT
TATTAGACCAAATTAATATGATGTCTTCCTTATCTCTTAGTCATGAAGAGTATCTTTATATGACTACTTTTCCATTTTTT
AAAAAAGAATATTTACATTGGTTAAAAAAATTTCGTTATAATATTACACAAGTTAAAGTTGATAATTATCATGGTCAACT
TCATATTCGTATAAGTGGATTATGGAAAGAAGTAATTTTATGGGAAGTCCCTATTTTATCATTAATTAGTGAAATTTTTC
ATAGACATTGTTATCCAGAAATTACTTCGAATATTGCAGTACAACATTTAAATAAAAAATTAAAAGAATTTTTTAAAAAA
AACAGAGATGTAGATTTATCTCGTTTAAAAATAGTAGATTTTGGAACAAGAAGACGATTTTCTTATGATGTACAATATTC
AATTATCAAAAGATTAAAAGATACATTTCCATTTTTAATTGGTTCAAGTAACTATCATATATCACGTATTTTAAAATTAT
TGCCAGTAGGTACCCAAGCTCATGAATGGTTTCAAGCACACCAACAAATTAGTTCCAATCTCAGAAATAGTCAAACATTA
GCATTAAAAACATGGTTGTCTCAATATAATCAACATTTAAGCATCGCTCTTACAGATTGTATTACAATGGATTCGTTTTT
ACGTGATTTTAATTTATTTTTTTCAAAATCTTATCAAGGTATCAGACATGATTCAGGAGATCCAGTAAAATGGGGTGAAA
AAGCTATTGAACATTATGAACGATTAGGTATTGATCCTACTACTAAAACGTTATTATTTTCAGATAATTTAAATTTTAGA
AAAATGATATCTCTTTATAAAAAATTTAATAATAGAGTAAATATTATATTTGGCATCGGAACAAAATTAACTTGTGATAT
TCCAAATGTTAAGCCGTTAAATATAGTAATCAAACTTGTCAAATGTAATGGTAAACCAGTTGCTAAATTATCTGATAGTC
CTGGTAAAACCTTTTGTCTAGATAGAAATTTTATTAAATCTTTATGTAAAGCATTTGATTTGTCTTTGACAGTGTAA

Upstream 100 bases:

>100_bases
ATTTTTTAATTTTTTAAATATTTTTATATAATTTTAAGTATAAAGTTATATCATAATTATCATACTATAAAAAATAATAC
TATAAAAAAATAGATATATT

Downstream 100 bases:

>100_bases
TTTAATACTAGTCACTTTGATTTGCTATTTTTCGATAGACTATTTAAAAATATACAAGGCAAAATTATGAGTAGAATATC
AATATCAGAAATTTATAAAG

Product: nicotinate phosphoribosyltransferase

Products: NA

Alternate protein names: NAPRTase

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKQYNYPIVKTLLDTDAYKFYMQQAVFYHYKNVDVVAEFICRGPNILGCYSHILLDQINMMSSLSLSHEEYLYMTTFPFF
KKEYLHWLKKFRYNITQVKVDNYHGQLHIRISGLWKEVILWEVPILSLISEIFHRHCYPEITSNIAVQHLNKKLKEFFKK
NRDVDLSRLKIVDFGTRRRFSYDVQYSIIKRLKDTFPFLIGSSNYHISRILKLLPVGTQAHEWFQAHQQISSNLRNSQTL
ALKTWLSQYNQHLSIALTDCITMDSFLRDFNLFFSKSYQGIRHDSGDPVKWGEKAIEHYERLGIDPTTKTLLFSDNLNFR
KMISLYKKFNNRVNIIFGIGTKLTCDIPNVKPLNIVIKLVKCNGKPVAKLSDSPGKTFCLDRNFIKSLCKAFDLSLTV

Sequences:

>Translated_398_residues
MKQYNYPIVKTLLDTDAYKFYMQQAVFYHYKNVDVVAEFICRGPNILGCYSHILLDQINMMSSLSLSHEEYLYMTTFPFF
KKEYLHWLKKFRYNITQVKVDNYHGQLHIRISGLWKEVILWEVPILSLISEIFHRHCYPEITSNIAVQHLNKKLKEFFKK
NRDVDLSRLKIVDFGTRRRFSYDVQYSIIKRLKDTFPFLIGSSNYHISRILKLLPVGTQAHEWFQAHQQISSNLRNSQTL
ALKTWLSQYNQHLSIALTDCITMDSFLRDFNLFFSKSYQGIRHDSGDPVKWGEKAIEHYERLGIDPTTKTLLFSDNLNFR
KMISLYKKFNNRVNIIFGIGTKLTCDIPNVKPLNIVIKLVKCNGKPVAKLSDSPGKTFCLDRNFIKSLCKAFDLSLTV
>Mature_398_residues
MKQYNYPIVKTLLDTDAYKFYMQQAVFYHYKNVDVVAEFICRGPNILGCYSHILLDQINMMSSLSLSHEEYLYMTTFPFF
KKEYLHWLKKFRYNITQVKVDNYHGQLHIRISGLWKEVILWEVPILSLISEIFHRHCYPEITSNIAVQHLNKKLKEFFKK
NRDVDLSRLKIVDFGTRRRFSYDVQYSIIKRLKDTFPFLIGSSNYHISRILKLLPVGTQAHEWFQAHQQISSNLRNSQTL
ALKTWLSQYNQHLSIALTDCITMDSFLRDFNLFFSKSYQGIRHDSGDPVKWGEKAIEHYERLGIDPTTKTLLFSDNLNFR
KMISLYKKFNNRVNIIFGIGTKLTCDIPNVKPLNIVIKLVKCNGKPVAKLSDSPGKTFCLDRNFIKSLCKAFDLSLTV

Specific function: NAD biosynthesis; nicotinamide to NaMN; second step. [C]

COG id: COG1488

COG function: function code H; Nicotinic acid phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAPRTase family

Homologues:

Organism=Escherichia coli, GI1787162, Length=394, Percent_Identity=58.8832487309645, Blast_Score=510, Evalue=1e-146,
Organism=Saccharomyces cerevisiae, GI6324783, Length=401, Percent_Identity=31.1720698254364, Blast_Score=185, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNCB_BUCAP (Q8K9I6)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660691.1
- ProteinModelPortal:   Q8K9I6
- SMR:   Q8K9I6
- EnsemblBacteria:   EBBUCT00000000086
- GeneID:   1005807
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg349
- GeneTree:   EBGT00050000007732
- HOGENOM:   HBG325503
- OMA:   GNTFYGK
- ProtClustDB:   PRK05321
- BioCyc:   BAPH198804:BUSG349-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00570
- InterPro:   IPR006406
- InterPro:   IPR015977
- InterPro:   IPR007229
- InterPro:   IPR002638
- PANTHER:   PTHR11098:SF1
- PIRSF:   PIRSF000484
- TIGRFAMs:   TIGR01514

Pfam domain/function: PF04095 NAPRTase; SSF51690 Q_phspho_trans

EC number: =2.4.2.11

Molecular weight: Translated: 46778; Mature: 46778

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYNYPIVKTLLDTDAYKFYMQQAVFYHYKNVDVVAEFICRGPNILGCYSHILLDQINM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MSSLSLSHEEYLYMTTFPFFKKEYLHWLKKFRYNITQVKVDNYHGQLHIRISGLWKEVIL
HHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEEHHHHHHHH
WEVPILSLISEIFHRHCYPEITSNIAVQHLNKKLKEFFKKNRDVDLSRLKIVDFGTRRRF
HHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCE
SYDVQYSIIKRLKDTFPFLIGSSNYHISRILKLLPVGTQAHEWFQAHQQISSNLRNSQTL
EECHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHH
ALKTWLSQYNQHLSIALTDCITMDSFLRDFNLFFSKSYQGIRHDSGDPVKWGEKAIEHYE
HHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
RLGIDPTTKTLLFSDNLNFRKMISLYKKFNNRVNIIFGIGTKLTCDIPNVKPLNIVIKLV
HCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCHHHHEEEEE
KCNGKPVAKLSDSPGKTFCLDRNFIKSLCKAFDLSLTV
ECCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKQYNYPIVKTLLDTDAYKFYMQQAVFYHYKNVDVVAEFICRGPNILGCYSHILLDQINM
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MSSLSLSHEEYLYMTTFPFFKKEYLHWLKKFRYNITQVKVDNYHGQLHIRISGLWKEVIL
HHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEEEHHHHHHHH
WEVPILSLISEIFHRHCYPEITSNIAVQHLNKKLKEFFKKNRDVDLSRLKIVDFGTRRRF
HHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCE
SYDVQYSIIKRLKDTFPFLIGSSNYHISRILKLLPVGTQAHEWFQAHQQISSNLRNSQTL
EECHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHH
ALKTWLSQYNQHLSIALTDCITMDSFLRDFNLFFSKSYQGIRHDSGDPVKWGEKAIEHYE
HHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
RLGIDPTTKTLLFSDNLNFRKMISLYKKFNNRVNIIFGIGTKLTCDIPNVKPLNIVIKLV
HCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCHHHHEEEEE
KCNGKPVAKLSDSPGKTFCLDRNFIKSLCKAFDLSLTV
ECCCCCCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438