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The map label for this gene is gyrA

Identifier: 21672462

GI number: 21672462

Start: 196355

End: 198832

Strand: Reverse

Name: gyrA

Synonym: BUsg174

Alternate gene names: 21672462

Gene position: 198832-196355 (Counterclockwise)

Preceding gene: 21672464

Following gene: 21672461

Centisome position: 31.0

GC content: 29.34

Gene sequence:

>2478_bases
ATGAAACACCTTGCTCGAGAAATTGTACAAGTCGATATTGAAAAAGAATTGAAAAGTTCTTACCTAGACTATGCTATGTC
TGTAATAGTAGGACGAGCCTTGCCAGATGTTCGAGATGGTTTAAAACCAGTCCATAGAAGAATACTTTTTGCAATGCATG
TATTAAGTAATTATTGGAATAAAGCATATAAAAAATCTGCTAGAGTGGTAGGAGATGTAATAGGTAAATATCATCCACAT
GGAGATTCTGCTGTATATGATTCAATCGTGCGAATGGCGCAAAATTTTTCATTACGTTATATGTTAATAGATGGTCAAGG
CAATTTTGGTTCAATTGACGGGGATGCAGCAGCAGCAATGCGATATACAGAAGTACGTATGTCTAAAATTTCTCAAGAGT
TGTTAGCTGATCTAGAAAAAAATACTGTAGAATTCATTCCGAATTATGATGGAACAGAACATATTCCAGAAATCCTGCCA
TCTAAAATACCAAATCTTTTAATTAATGGATCATCAGGAATAGCGGTGGGTATGGCAACGAATATTCCTCCTCACAACTT
GAATGAGGTAATTGACGGATGTTTAGCTTATATAAAAGATGATAACATTACACTACGAGAATTAATTCAACATATTCCAG
GACCAGATTTTCCAACTGCAGGAATTATCAATGGTAAAAAGGGAATTGAAGAAGCATATCGAACAGGAAAGGGAAAGATT
TATATTCGTGCTCGAAACAAAATTGAAAAAAATAAAAAAAACAAAAAAGAATCAATTATTTTTTATGAAATTCCTTATCA
AGTTAACAAATCACGTTTAATTGAAAAAATAGCAGAACTAGTAAAGGAAAAAAGAATTGATGGAATAACTGCTTTACGTG
ACGAATCAGACAAAGATGGAATGAGAATTGTAGTTGAAATAAAAAAAGAAGCTATGTCAGAAATAATTTTAAATCAACTA
TATTCTCTTACTCAACTACAAATTTCTTTTGGTATAAATATGGTTGCATTATGCCAAGGACAACCAAAAACATTATCTTT
AAAAGAGATATTAAAACATTTTTTATCACATAGAGAAGAAATAGTTACACGCCGCAGTATTTTTGAATTAGATAAAACTT
GTAAAAGATCTCATATTCTTGAAGGATTAAGTATAGCTTTAATAAACATTAACTGTATTATTGATCTAATAAAAAAAGCT
AAAAGTTCATTAGAAGCAAAAGAGTTAATAATAAAAAAAACGTGGATTTATAAAAATTCAGAAAAAATAAATCAAAAAGA
TCATGATTATCATTTTACTGAAGAACAAGTGCAAGCTATTTTAGATTTACGCTTACATAAATTAACAAGTTTAGAAAAAA
ATAAACTTGTTTTAGAATACGATTGTTTAACTAAAAAAATCAAAGAATTAAAAAAAATTTTAAAAAGTCGCGATCGTATG
CGAGAAGTTATTAGAGATGAATTAGTATCAATAAAAAATAATTTCGGAGATCAGAGACGAACAGAAATTACAGAAAATAA
TCCTGATATTAGCATTGAAGATTTAATTAATCAAGAAGATGTAGTAGTAACATTATCTCATTCTGGATACGTTAAATATC
AACCTCTTTCTGATTATAATGCTCAAAAAAGAGGAGGAAAGGGAAAATCAGCAGCTAAAATTAAAGAAGAAGATTTTATA
GAAAGTTTAGTCGTGACGAATACACATGATACTATACTATGTTTTTCCAGTCGAGGTATCCTATATTGGATGAAAGTTTA
TCAACTACCAGAATCTAGTAGACATGCAAAAGGTAGACCCATAGTCAATTTGTTACCATTAAGTAACAAGGAAAGAATTA
CAGCAATACTTCCTATTCATGAATTTAAAGATAATCTAAATATTTTTATGGCTACTGCACAAGGAATAGTTAAAAAAAGT
TCTTTAAAAGAATTTAAAAAACCTAGAATTGCAGGCATTATAGCAATTAATTTACGAGCTAATGATGAACTTATTGGAGT
TTCATTAACAAATGGTAAGAACAATATCATGTTATTTACACAAAATGGAAAAGTGGTTCAATTTTTAGAAAATAGTGTTA
GAACAATGGGAAGAACAGCTTCTGGAGTAAGAGGAATTAAAATTTCTAAAAATGACAAAGTAGTATCCCTTATTGTTCCG
TATAAAAAAGAAAGTATTTTAATAGCAACAAATAACGGCTATGGAAAACGCACAAAAATAACTGATTTTCCAATTAAATC
ACGTGCGACACAAGGTGTAATTTCAATAAAAATTACTAAAAAGAACGGAAAAATAATTGGAGCTATACAAGTGGTAGAAA
AAGATCAAATTATGATGATTACTAACGCAGGGACACTAGTTAGAATTAGAGCGTCAGAAATAGGTGTTTTAAAAAGAAAT
ACACAAGGTGTCATCTTAATAAGAACATCAAAAAATGAAAAAGTTGTTGCTTTACAAAGAATTGTAGAACCTATATAA

Upstream 100 bases:

>100_bases
TAGAATCAGTTCTTTCAATGCATCTGACACTTTAACTATGTTATAATTTTATACTCAATATAATTCTATTAATCAACGCA
AAAAAGAGAAAATAGTTAAT

Downstream 100 bases:

>100_bases
AAATATTTTAATAAAAAACCATTTTAACTAAAAGAGTTTTTATACTACATTTCAAAAACAATATTTTTATATATATTTTT
TTAAATAGGTTAATTTAAAT

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 825; Mature: 825

Protein sequence:

>825_residues
MKHLAREIVQVDIEKELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRILFAMHVLSNYWNKAYKKSARVVGDVIGKYHPH
GDSAVYDSIVRMAQNFSLRYMLIDGQGNFGSIDGDAAAAMRYTEVRMSKISQELLADLEKNTVEFIPNYDGTEHIPEILP
SKIPNLLINGSSGIAVGMATNIPPHNLNEVIDGCLAYIKDDNITLRELIQHIPGPDFPTAGIINGKKGIEEAYRTGKGKI
YIRARNKIEKNKKNKKESIIFYEIPYQVNKSRLIEKIAELVKEKRIDGITALRDESDKDGMRIVVEIKKEAMSEIILNQL
YSLTQLQISFGINMVALCQGQPKTLSLKEILKHFLSHREEIVTRRSIFELDKTCKRSHILEGLSIALININCIIDLIKKA
KSSLEAKELIIKKTWIYKNSEKINQKDHDYHFTEEQVQAILDLRLHKLTSLEKNKLVLEYDCLTKKIKELKKILKSRDRM
REVIRDELVSIKNNFGDQRRTEITENNPDISIEDLINQEDVVVTLSHSGYVKYQPLSDYNAQKRGGKGKSAAKIKEEDFI
ESLVVTNTHDTILCFSSRGILYWMKVYQLPESSRHAKGRPIVNLLPLSNKERITAILPIHEFKDNLNIFMATAQGIVKKS
SLKEFKKPRIAGIIAINLRANDELIGVSLTNGKNNIMLFTQNGKVVQFLENSVRTMGRTASGVRGIKISKNDKVVSLIVP
YKKESILIATNNGYGKRTKITDFPIKSRATQGVISIKITKKNGKIIGAIQVVEKDQIMMITNAGTLVRIRASEIGVLKRN
TQGVILIRTSKNEKVVALQRIVEPI

Sequences:

>Translated_825_residues
MKHLAREIVQVDIEKELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRILFAMHVLSNYWNKAYKKSARVVGDVIGKYHPH
GDSAVYDSIVRMAQNFSLRYMLIDGQGNFGSIDGDAAAAMRYTEVRMSKISQELLADLEKNTVEFIPNYDGTEHIPEILP
SKIPNLLINGSSGIAVGMATNIPPHNLNEVIDGCLAYIKDDNITLRELIQHIPGPDFPTAGIINGKKGIEEAYRTGKGKI
YIRARNKIEKNKKNKKESIIFYEIPYQVNKSRLIEKIAELVKEKRIDGITALRDESDKDGMRIVVEIKKEAMSEIILNQL
YSLTQLQISFGINMVALCQGQPKTLSLKEILKHFLSHREEIVTRRSIFELDKTCKRSHILEGLSIALININCIIDLIKKA
KSSLEAKELIIKKTWIYKNSEKINQKDHDYHFTEEQVQAILDLRLHKLTSLEKNKLVLEYDCLTKKIKELKKILKSRDRM
REVIRDELVSIKNNFGDQRRTEITENNPDISIEDLINQEDVVVTLSHSGYVKYQPLSDYNAQKRGGKGKSAAKIKEEDFI
ESLVVTNTHDTILCFSSRGILYWMKVYQLPESSRHAKGRPIVNLLPLSNKERITAILPIHEFKDNLNIFMATAQGIVKKS
SLKEFKKPRIAGIIAINLRANDELIGVSLTNGKNNIMLFTQNGKVVQFLENSVRTMGRTASGVRGIKISKNDKVVSLIVP
YKKESILIATNNGYGKRTKITDFPIKSRATQGVISIKITKKNGKIIGAIQVVEKDQIMMITNAGTLVRIRASEIGVLKRN
TQGVILIRTSKNEKVVALQRIVEPI
>Mature_825_residues
MKHLAREIVQVDIEKELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRILFAMHVLSNYWNKAYKKSARVVGDVIGKYHPH
GDSAVYDSIVRMAQNFSLRYMLIDGQGNFGSIDGDAAAAMRYTEVRMSKISQELLADLEKNTVEFIPNYDGTEHIPEILP
SKIPNLLINGSSGIAVGMATNIPPHNLNEVIDGCLAYIKDDNITLRELIQHIPGPDFPTAGIINGKKGIEEAYRTGKGKI
YIRARNKIEKNKKNKKESIIFYEIPYQVNKSRLIEKIAELVKEKRIDGITALRDESDKDGMRIVVEIKKEAMSEIILNQL
YSLTQLQISFGINMVALCQGQPKTLSLKEILKHFLSHREEIVTRRSIFELDKTCKRSHILEGLSIALININCIIDLIKKA
KSSLEAKELIIKKTWIYKNSEKINQKDHDYHFTEEQVQAILDLRLHKLTSLEKNKLVLEYDCLTKKIKELKKILKSRDRM
REVIRDELVSIKNNFGDQRRTEITENNPDISIEDLINQEDVVVTLSHSGYVKYQPLSDYNAQKRGGKGKSAAKIKEEDFI
ESLVVTNTHDTILCFSSRGILYWMKVYQLPESSRHAKGRPIVNLLPLSNKERITAILPIHEFKDNLNIFMATAQGIVKKS
SLKEFKKPRIAGIIAINLRANDELIGVSLTNGKNNIMLFTQNGKVVQFLENSVRTMGRTASGVRGIKISKNDKVVSLIVP
YKKESILIATNNGYGKRTKITDFPIKSRATQGVISIKITKKNGKIIGAIQVVEKDQIMMITNAGTLVRIRASEIGVLKRN
TQGVILIRTSKNEKVVALQRIVEPI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family

Homologues:

Organism=Homo sapiens, GI19913408, Length=345, Percent_Identity=26.0869565217391, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI19913406, Length=320, Percent_Identity=26.875, Blast_Score=81, Evalue=5e-15,
Organism=Escherichia coli, GI1788562, Length=843, Percent_Identity=66.429418742586, Blast_Score=1176, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=680, Percent_Identity=35.4411764705882, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=167, Percent_Identity=32.9341317365269, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI212645657, Length=166, Percent_Identity=32.5301204819277, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI212645845, Length=181, Percent_Identity=31.4917127071823, Blast_Score=70, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=176, Percent_Identity=28.9772727272727, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRA_BUCAP (Q8K9W2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660529.1
- ProteinModelPortal:   Q8K9W2
- EnsemblBacteria:   EBBUCT00000000275
- GeneID:   1005371
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg174
- GeneTree:   EBGT00050000007782
- HOGENOM:   HBG635183
- OMA:   ELGNDWN
- ProtClustDB:   PRK05560
- BioCyc:   BAPH198804:BUSG174-MONOMER
- GO:   GO:0005694
- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013760
- Gene3D:   G3DSA:3.30.1360.40
- Gene3D:   G3DSA:3.90.199.10
- SMART:   SM00434
- TIGRFAMs:   TIGR01063

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 93366; Mature: 93366

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: ACT_SITE 122-122

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLAREIVQVDIEKELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRILFAMHVLSNYWN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KAYKKSARVVGDVIGKYHPHGDSAVYDSIVRMAQNFSLRYMLIDGQGNFGSIDGDAAAAM
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHH
RYTEVRMSKISQELLADLEKNTVEFIPNYDGTEHIPEILPSKIPNLLINGSSGIAVGMAT
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEC
NIPPHNLNEVIDGCLAYIKDDNITLRELIQHIPGPDFPTAGIINGKKGIEEAYRTGKGKI
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEE
YIRARNKIEKNKKNKKESIIFYEIPYQVNKSRLIEKIAELVKEKRIDGITALRDESDKDG
EEEECCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
MRIVVEIKKEAMSEIILNQLYSLTQLQISFGINMVALCQGQPKTLSLKEILKHFLSHREE
CEEEEEEHHHHHHHHHHHHHHHHHHEEEHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
IVTRRSIFELDKTCKRSHILEGLSIALININCIIDLIKKAKSSLEAKELIIKKTWIYKNS
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHEEECCC
EKINQKDHDYHFTEEQVQAILDLRLHKLTSLEKNKLVLEYDCLTKKIKELKKILKSRDRM
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
REVIRDELVSIKNNFGDQRRTEITENNPDISIEDLINQEDVVVTLSHSGYVKYQPLSDYN
HHHHHHHHHHHHHCCCCCHHCCCCCCCCCEEHHHHCCCCCEEEEEECCCEEEECCCCCCC
AQKRGGKGKSAAKIKEEDFIESLVVTNTHDTILCFSSRGILYWMKVYQLPESSRHAKGRP
CHHCCCCCCCCHHCCHHHHHHHHHEECCCCEEEEEECCCEEEEEHHHHCCCCCCCCCCCC
IVNLLPLSNKERITAILPIHEFKDNLNIFMATAQGIVKKSSLKEFKKPRIAGIIAINLRA
EEEEECCCCCCCEEEEEEHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCEEEEEEEEEEC
NDELIGVSLTNGKNNIMLFTQNGKVVQFLENSVRTMGRTASGVRGIKISKNDKVVSLIVP
CCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEC
YKKESILIATNNGYGKRTKITDFPIKSRATQGVISIKITKKNGKIIGAIQVVEKDQIMMI
CCCCEEEEEECCCCCCCCEEECCCCCCCCCCCEEEEEEEECCCEEEEEEEEECCCCEEEE
TNAGTLVRIRASEIGVLKRNTQGVILIRTSKNEKVVALQRIVEPI
ECCCCEEEEECCCCCEEEECCCEEEEEEECCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKHLAREIVQVDIEKELKSSYLDYAMSVIVGRALPDVRDGLKPVHRRILFAMHVLSNYWN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KAYKKSARVVGDVIGKYHPHGDSAVYDSIVRMAQNFSLRYMLIDGQGNFGSIDGDAAAAM
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHH
RYTEVRMSKISQELLADLEKNTVEFIPNYDGTEHIPEILPSKIPNLLINGSSGIAVGMAT
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEC
NIPPHNLNEVIDGCLAYIKDDNITLRELIQHIPGPDFPTAGIINGKKGIEEAYRTGKGKI
CCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEE
YIRARNKIEKNKKNKKESIIFYEIPYQVNKSRLIEKIAELVKEKRIDGITALRDESDKDG
EEEECCHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
MRIVVEIKKEAMSEIILNQLYSLTQLQISFGINMVALCQGQPKTLSLKEILKHFLSHREE
CEEEEEEHHHHHHHHHHHHHHHHHHEEEHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
IVTRRSIFELDKTCKRSHILEGLSIALININCIIDLIKKAKSSLEAKELIIKKTWIYKNS
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHEEECCC
EKINQKDHDYHFTEEQVQAILDLRLHKLTSLEKNKLVLEYDCLTKKIKELKKILKSRDRM
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
REVIRDELVSIKNNFGDQRRTEITENNPDISIEDLINQEDVVVTLSHSGYVKYQPLSDYN
HHHHHHHHHHHHHCCCCCHHCCCCCCCCCEEHHHHCCCCCEEEEEECCCEEEECCCCCCC
AQKRGGKGKSAAKIKEEDFIESLVVTNTHDTILCFSSRGILYWMKVYQLPESSRHAKGRP
CHHCCCCCCCCHHCCHHHHHHHHHEECCCCEEEEEECCCEEEEEHHHHCCCCCCCCCCCC
IVNLLPLSNKERITAILPIHEFKDNLNIFMATAQGIVKKSSLKEFKKPRIAGIIAINLRA
EEEEECCCCCCCEEEEEEHHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCEEEEEEEEEEC
NDELIGVSLTNGKNNIMLFTQNGKVVQFLENSVRTMGRTASGVRGIKISKNDKVVSLIVP
CCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEC
YKKESILIATNNGYGKRTKITDFPIKSRATQGVISIKITKKNGKIIGAIQVVEKDQIMMI
CCCCEEEEEECCCCCCCCEEECCCCCCCCCCCEEEEEEEECCCEEEEEEEEECCCCEEEE
TNAGTLVRIRASEIGVLKRNTQGVILIRTSKNEKVVALQRIVEPI
ECCCCEEEEECCCCCEEEECCCEEEEEEECCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438