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The map label for this gene is murB

Identifier: 21672335

GI number: 21672335

Start: 49078

End: 50112

Strand: Reverse

Name: murB

Synonym: BUsg042

Alternate gene names: 21672335

Gene position: 50112-49078 (Counterclockwise)

Preceding gene: 21672337

Following gene: 21672334

Centisome position: 7.81

GC content: 22.51

Gene sequence:

>1035_bases
ATGTATAAAAAAAAAAAATCTTACCAATCTTTAAAAAATTTAAATACATTTTCAATAAATGTTACAGCAAAAAAAATTAT
TTTTGTTAAGACAATTCAATCATTAATGAAAATATGGAAAACATGTAATTTATCTAATATTCCTTATATAATTTTAGGCG
AAGGAAGCAATGTACTTTTTTTAGAAAATTATGCAGGAGTAGTCATTATTAATCGCATAAAGGGAATAAGAATTGAAGAA
AAAAAAAAAAATTGGTTATTACATGTTTTTTCAGGAGAAAAATGGCATGATTTAGTTAAATATACATTGAGAATGGGTTT
CTTCGGATTAGAAAATTTAGCTTTAATTCCTGGTTCAGTAGGATCAGCAGCTATTCAAAATATTGGTGCTTATGGTTTAG
AGTTAAAAAATATATGCCAGTATGTAGATGTTATTTCTCTAGAAAATGGCAAAACAATTAGACTTAAAAAAAAAACATGT
AATTTTTCTTATCGAAGCAGTATTTTTAAATACAAATATAATAACGGATATGCAGTTATTGCAGTTGGTATAAAAATAAA
AAAAAACTGGAAACCTGTTATTTTTTCATCACTTTTAAAAAGTAAAAAAATATTAGAAATAAATGCTTATAAAATATTTA
ATATAGTCTGTCAGATAAGAAAAAAAAAATTACCTAATCTTAAAAAATTAGGAAATGCAGGTAGTTTTTTTAAAAATCCT
ATTATTACATCTAAAAAAACAAAAAAAATTTTATCTTCATACATGAAGATGCCATATTACATCCAAAAAAATGGATTTAT
CAAAATTCCTGCTGCTTGGTTAATTGAAAAGTATAATTTTAAAAATATTCAAATTGGTGATGCAGCAATTTATAAAAAAC
AAAAACTTATATTAATTAATCTTAAAAAAGCAAATTCCAAAGACATTTTAAAATTAGCTCAAATAATACAAAAATGCATT
CTAAAAAAATTTGGAATATACTTAGAACCTGAGGTAGATTTTATTAATTCTTTAGGAAAGGTAAAATTATTGTAA

Upstream 100 bases:

>100_bases
TAATATTTTTGATATATTATACATTAAATTTATATTAACTTTTTAAAGTTTAAAGAATTAAATTTTTATTTAAAAAATAA
AAAAACTACGTTAAAACTTT

Downstream 100 bases:

>100_bases
AATACCTCTTATATACTTAACACTCATAATAATTTAACTAAAAAATTTTTTCTTAAAAAACTTGCAATAAAAATGAAAAA
TTATATAATATAAAAAGAAA

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MYKKKKSYQSLKNLNTFSINVTAKKIIFVKTIQSLMKIWKTCNLSNIPYIILGEGSNVLFLENYAGVVIINRIKGIRIEE
KKKNWLLHVFSGEKWHDLVKYTLRMGFFGLENLALIPGSVGSAAIQNIGAYGLELKNICQYVDVISLENGKTIRLKKKTC
NFSYRSSIFKYKYNNGYAVIAVGIKIKKNWKPVIFSSLLKSKKILEINAYKIFNIVCQIRKKKLPNLKKLGNAGSFFKNP
IITSKKTKKILSSYMKMPYYIQKNGFIKIPAAWLIEKYNFKNIQIGDAAIYKKQKLILINLKKANSKDILKLAQIIQKCI
LKKFGIYLEPEVDFINSLGKVKLL

Sequences:

>Translated_344_residues
MYKKKKSYQSLKNLNTFSINVTAKKIIFVKTIQSLMKIWKTCNLSNIPYIILGEGSNVLFLENYAGVVIINRIKGIRIEE
KKKNWLLHVFSGEKWHDLVKYTLRMGFFGLENLALIPGSVGSAAIQNIGAYGLELKNICQYVDVISLENGKTIRLKKKTC
NFSYRSSIFKYKYNNGYAVIAVGIKIKKNWKPVIFSSLLKSKKILEINAYKIFNIVCQIRKKKLPNLKKLGNAGSFFKNP
IITSKKTKKILSSYMKMPYYIQKNGFIKIPAAWLIEKYNFKNIQIGDAAIYKKQKLILINLKKANSKDILKLAQIIQKCI
LKKFGIYLEPEVDFINSLGKVKLL
>Mature_344_residues
MYKKKKSYQSLKNLNTFSINVTAKKIIFVKTIQSLMKIWKTCNLSNIPYIILGEGSNVLFLENYAGVVIINRIKGIRIEE
KKKNWLLHVFSGEKWHDLVKYTLRMGFFGLENLALIPGSVGSAAIQNIGAYGLELKNICQYVDVISLENGKTIRLKKKTC
NFSYRSSIFKYKYNNGYAVIAVGIKIKKNWKPVIFSSLLKSKKILEINAYKIFNIVCQIRKKKLPNLKKLGNAGSFFKNP
IITSKKTKKILSSYMKMPYYIQKNGFIKIPAAWLIEKYNFKNIQIGDAAIYKKQKLILINLKKANSKDILKLAQIIQKCI
LKKFGIYLEPEVDFINSLGKVKLL

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=336, Percent_Identity=47.9166666666667, Blast_Score=357, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_BUCAP (Q8KA63)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660402.1
- ProteinModelPortal:   Q8KA63
- SMR:   Q8KA63
- EnsemblBacteria:   EBBUCT00000000119
- GeneID:   1005858
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg042
- GeneTree:   EBGT00050000007998
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- ProtClustDB:   PRK00046
- BioCyc:   BAPH198804:BUSG042-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 39519; Mature: 39519

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 165-165 ACT_SITE 235-235 ACT_SITE 331-331

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKKKKSYQSLKNLNTFSINVTAKKIIFVKTIQSLMKIWKTCNLSNIPYIILGEGSNVLF
CCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEE
LENYAGVVIINRIKGIRIEEKKKNWLLHVFSGEKWHDLVKYTLRMGFFGLENLALIPGSV
EECCCCEEEEEECCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEECCCCC
GSAAIQNIGAYGLELKNICQYVDVISLENGKTIRLKKKTCNFSYRSSIFKYKYNNGYAVI
CHHHHHHCCCCCCCHHHHHHHHHEEEECCCCEEEEEECCCCCHHHCCEEEEEECCCEEEE
AVGIKIKKNWKPVIFSSLLKSKKILEINAYKIFNIVCQIRKKKLPNLKKLGNAGSFFKNP
EEEEEEECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHCCC
IITSKKTKKILSSYMKMPYYIQKNGFIKIPAAWLIEKYNFKNIQIGDAAIYKKQKLILIN
CCCCHHHHHHHHHHHCCCEEEECCCEEEECHHHEEEECCCCEEEECCHHHCCCCEEEEEE
LKKANSKDILKLAQIIQKCILKKFGIYLEPEVDFINSLGKVKLL
ECCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCCCEEEC
>Mature Secondary Structure
MYKKKKSYQSLKNLNTFSINVTAKKIIFVKTIQSLMKIWKTCNLSNIPYIILGEGSNVLF
CCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEE
LENYAGVVIINRIKGIRIEEKKKNWLLHVFSGEKWHDLVKYTLRMGFFGLENLALIPGSV
EECCCCEEEEEECCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEEECCCCC
GSAAIQNIGAYGLELKNICQYVDVISLENGKTIRLKKKTCNFSYRSSIFKYKYNNGYAVI
CHHHHHHCCCCCCCHHHHHHHHHEEEECCCCEEEEEECCCCCHHHCCEEEEEECCCEEEE
AVGIKIKKNWKPVIFSSLLKSKKILEINAYKIFNIVCQIRKKKLPNLKKLGNAGSFFKNP
EEEEEEECCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHCCC
IITSKKTKKILSSYMKMPYYIQKNGFIKIPAAWLIEKYNFKNIQIGDAAIYKKQKLILIN
CCCCHHHHHHHHHHHCCCEEEECCCEEEECHHHEEEECCCCEEEECCHHHCCCCEEEEEE
LKKANSKDILKLAQIIQKCILKKFGIYLEPEVDFINSLGKVKLL
ECCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438