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The map label for this gene is nodL [H]

Identifier: 21244867

GI number: 21244867

Start: 4877137

End: 4877715

Strand: Reverse

Name: nodL [H]

Synonym: XAC4150

Alternate gene names: 21244867

Gene position: 4877715-4877137 (Counterclockwise)

Preceding gene: 21244868

Following gene: 21244866

Centisome position: 94.25

GC content: 66.84

Gene sequence:

>579_bases
ATGCAGACCGAAAAACAGAAAATGCTGGCAGGCGAACTCTACAACGCCGCCGATGCGCAGCTGCAGGCCGATCAGGCGGC
AGCTGCGGTGTGGATGGAGCGCTACAACGCCACTGGCGCGCAGTCATCGGCGCAGCGGCATGCATTGCTGGTCGAGCGGC
TCGCCGAGGTGGGTGCGAATGCGGTGATCCGGCCGCCGTTTCATTGCGATTACGGCTACAACATCCATCTGGGCGCGGGC
GTGTTCCTCAACTTCAATTGCGTGATCCTGGATATCTGCCAGGTGTACATCGGCGATGGCACGCAGGTGGGGCCAGCGGT
GCAGTTCTACGCTGCCGACCATCCGCGCGATGCCGCCGGACGTGCCAGCGGGCTGGAGTTCGGGCGGCCAATCCGCGTCG
GGCGCAATGTGTGGATTGGGGGCGGGGCGATCATCCTGCCGGGCGTCTGCATCGGCGACGATGCGGTAATCGGTGCCGGT
GCGGTCGTCACGCGCGATGTGCCGGCCGGTGCGATCGCCGTGGGCAATCCGGCGCGCGTGCGCGCACCCCGGCTTGCCGA
TGCGCCGCCCACCGACTGA

Upstream 100 bases:

>100_bases
CTTTGAGCCGGCAATCGGGGCCATTGGTGCATCGCTCGATGCATACCGCGCTACATCCATGCCACCCTGTCAGCAGTTCA
TTGCATAGGTGCCCGGCGGC

Downstream 100 bases:

>100_bases
ACGCAACGAAGAAGAGCCGCTACAAAAAGCGATCGCGCAGCTGCCAGGCGGACGCGGCCGGTGCGATGCAGCGCATACGC
GAACAGAGCAGTATCGAACG

Product: nodulation protein

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MQTEKQKMLAGELYNAADAQLQADQAAAAVWMERYNATGAQSSAQRHALLVERLAEVGANAVIRPPFHCDYGYNIHLGAG
VFLNFNCVILDICQVYIGDGTQVGPAVQFYAADHPRDAAGRASGLEFGRPIRVGRNVWIGGGAIILPGVCIGDDAVIGAG
AVVTRDVPAGAIAVGNPARVRAPRLADAPPTD

Sequences:

>Translated_192_residues
MQTEKQKMLAGELYNAADAQLQADQAAAAVWMERYNATGAQSSAQRHALLVERLAEVGANAVIRPPFHCDYGYNIHLGAG
VFLNFNCVILDICQVYIGDGTQVGPAVQFYAADHPRDAAGRASGLEFGRPIRVGRNVWIGGGAIILPGVCIGDDAVIGAG
AVVTRDVPAGAIAVGNPARVRAPRLADAPPTD
>Mature_192_residues
MQTEKQKMLAGELYNAADAQLQADQAAAAVWMERYNATGAQSSAQRHALLVERLAEVGANAVIRPPFHCDYGYNIHLGAG
VFLNFNCVILDICQVYIGDGTQVGPAVQFYAADHPRDAAGRASGLEFGRPIRVGRNVWIGGGAIILPGVCIGDDAVIGAG
AVVTRDVPAGAIAVGNPARVRAPRLADAPPTD

Specific function: Acetyltransferase implicated in the O-acetylation of Nod factors [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=180, Percent_Identity=49.4444444444444, Blast_Score=189, Evalue=1e-49,
Organism=Escherichia coli, GI1786537, Length=175, Percent_Identity=37.7142857142857, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788367, Length=173, Percent_Identity=27.7456647398844, Blast_Score=60, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=176, Percent_Identity=41.4772727272727, Blast_Score=151, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 20104; Mature: 20104

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTEKQKMLAGELYNAADAQLQADQAAAAVWMERYNATGAQSSAQRHALLVERLAEVGAN
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
AVIRPPFHCDYGYNIHLGAGVFLNFNCVILDICQVYIGDGTQVGPAVQFYAADHPRDAAG
CEECCCCCCCCCCEEEECCEEEEECCCCEEEEHHHHCCCCCCCCCEEEEEECCCCCCCCC
RASGLEFGRPIRVGRNVWIGGGAIILPGVCIGDDAVIGAGAVVTRDVPAGAIAVGNPARV
CCCCCCCCCCEECCCEEEEECCEEEECCEEECCCCEECCCEEEEECCCCCEEEECCCCCC
RAPRLADAPPTD
CCCCCCCCCCCC
>Mature Secondary Structure
MQTEKQKMLAGELYNAADAQLQADQAAAAVWMERYNATGAQSSAQRHALLVERLAEVGAN
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
AVIRPPFHCDYGYNIHLGAGVFLNFNCVILDICQVYIGDGTQVGPAVQFYAADHPRDAAG
CEECCCCCCCCCCEEEECCEEEEECCCCEEEEHHHHCCCCCCCCCEEEEEECCCCCCCCC
RASGLEFGRPIRVGRNVWIGGGAIILPGVCIGDDAVIGAGAVVTRDVPAGAIAVGNPARV
CCCCCCCCCCEECCCEEEEECCEEEECCEEECCCCEECCCEEEEECCCCCEEEECCCCCC
RAPRLADAPPTD
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 90 {maltose}} 62 {glucose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1558960; 8801423; 11481432 [H]