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The map label for this gene is topA [H]

Identifier: 21244531

GI number: 21244531

Start: 4477778

End: 4480270

Strand: Direct

Name: topA [H]

Synonym: XAC3807

Alternate gene names: 21244531

Gene position: 4477778-4480270 (Clockwise)

Preceding gene: 77748743

Following gene: 21244532

Centisome position: 86.52

GC content: 64.74

Gene sequence:

>2493_bases
ATGCCCAAGCACCTGCTCATCGTCGAATCGCCCGCCAAGGCCAAGACGATCAATAAATACCTCGGCAAGGACTTCACCGT
CCTGGCCTCGTATGGGCACGTGCGCGACCTGGTCCCCAAGGAAGGCGCGGTCGATCCGGACAACGGCTTTGCGATGCGTT
ACGACCTCATCGAGAAGAACGAGAAGCACGTCGAAGCCATCGCGCGTGCCGCCAAGGGTGCCGACGACATCTTTCTGGCG
ACCGACCCGGACCGCGAGGGCGAGGCGATCAGCTGGCACATCGCCGAGATCCTGAAGGAGCGCGGCCTGCTCAAGGACAA
GCCGATGCAGCGGGTGGTGTTCACCGAAATCACCCCGCGTGCGATCAAGGAGGCGATGGCCAAGCCACGCATGATCGCCG
GCGACCTGGTGGATGCGCAGCAGGCGCGCCGCGCGCTCGATTACCTGGTCGGCTTCAATCTGTCGCCGGTGCTGTGGCGC
AAGGTGCAGCGCGGCCTGTCCGCTGGCCGCGTGCAATCGCCGGCGCTGCGTATGATCGTCGAGCGCGAAGAAGAGATCGA
AGCCTTCATCCCGCGCGAGTACTGGTCCATCGATGCGCATTGCCGGCATCCCTCGCAGGCGTTCAACGCGCGACTGATCA
AGCTGGATGGGCAGAAGTTCGAGCAGTTCACCGTGACCGACGGCGACACCGCCGAGGCGGCGCGCCTGCGCATCCAGCAG
GCCGCGCAAGGCGTGCTGCATGTCACCGACGTGGCCAGCAAGGAGCGCAAGCGCCGCCCTGCCCCGCCGTTCACGACCTC
CACGCTGCAGCAGGAAGCCTCGCGCAAGCTCGGCTTCACCACCCGCAAGACCATGCAGGTGGCGCAGAAGTTGTACGAAG
GCGTGGCACTCGGCGACGAAGGCTCGGTCGGTCTGATCAGCTATATGCGTACCGACTCGGTGAACCTGTCGCAGGACGCC
CTGGCGGAAATCCGCGATGTGATCGCGCGCGACTTCGGCACCGCTTCGCTCCCCGACCAGCCCAACGCCTACACCACCAA
ATCCAAGAACGCGCAGGAAGCCCACGAAGCGGTGCGCCCGACCTCCGCGCTGCGCACCCCGGCGCAGGTGGCGCGTTTCC
TGTCCGAGGACGAGCGCCGCCTGTACGAGCTGATCTGGCGCCGCGCAGTGGCCTGCCAGATGATTCCGGCCACGCTCAAT
ACCGTCAGCGTGGATCTGTCGGCCGGCAGCGAGCACGTGTTCCGCGCCAGCGGCACCACCGTGGTGGTCCCGGGCTTCCT
GGCCGTGTACGAAGAAGGCAAGGACACCAAGAGCAGCGAGGACGAGGACGAAGGCCGCAAGCTGCCGCTGATGAAGGCCG
GCGACAACGTCCCGCTGGACCGCATCGTCACCGACCAGCACTTCACCCAGCCGCCGCCGCGTTTCACCGAAGCGGCGCTG
GTCAAGGCGCTGGAGGAATACGGCATCGGCCGTCCCTCCACCTACGCCTCGATCATCCAGACCCTGCAGTTCCGCAAATA
CGTCGAGATGGAAGGCCGCAGCTTCCGTCCCACCGACGTGGGCCGTGCAGTGTCCAAGTTCCTGTCCGGGCATTTCACCC
GCTACGTCGATTACGACTTCACCGCCAATCTGGAAGACGATCTGGACGCGGTGTCGCGCGGCGAAGCCGAGTGGATCCCG
TTGATGGAGAAGTTCTGGGGCCCGTTCAAGGAATTGGTCGAAGACAAGAAAGACTCGCTGGACAAGACCGATGCCGGCAG
CGTGCGCGTGCTCGGCACCGACCCGGTCAGCGGCAAGGAAGTCAGCGCGCGCATCGGTCGCTTCGGCCCGATGGTGCAGA
TCGGCACGGTCGAAGACGAGGACAAGCCCACCTTCGCGTCGCTGCGTCCGGGCCAGAGCATCTATTCGATCTCCCTCGAA
GATGCGCTGGAACTGTTCAAGATGCCGCGCGCGCTGGGCCAGGACAAGGACCAGGATGTCAGCGTGGGCATCGGCCGTTT
CGGCCCGTTCGCGCGGCGCGGCAGCACCTATGCCTCGCTGAAGAAGGAAGACGACCCCTACACCATCGACCTGGCGCGCG
CCATCTTCCTGATCGAAGAGAAGGAAGAGATTGCACGCAACCGGGTGATCAAGGAATTCGACGGCAGCGACATCCAGGTG
CTCAACGGCCGCTTCGGCCCCTACATCAGCGACGGCAAGCTCAATGGCAAGATCCCCAAGGACCGTGAGCCGGCGTCGTT
GACGTTCGAAGAAGTGCAGCAGCTGCTGGCCGACACCGGCAAGCCGGTGCGCAAGGGCTTCGGCGCCAAGAAGGCCACCC
TGAAGAAGAATACGGTCAAGGATTCGGCGCCGAAGAAGCCTGCCGTCAAGAAGACCGCGACCAAGACCGCGGCCAGCAAG
ACCGCTGTCAAGAAGGCGCCAGCCAAGAAGACGGCCACCAAGAAGGCCGCCAAGCGCGTGGTCAAGAAAACCGTGAGCAA
GGCCGCGGGCTGA

Upstream 100 bases:

>100_bases
CCGGGCGTTAACGCGTGGGAAACGGTCGATCCGCTTCCCTCGACCGGCCAATTAGGCCACGCTACCCGCCCCCCGGGCTC
CGCCCCAACAAGTTGCCACC

Downstream 100 bases:

>100_bases
GAGGCGCAGCATGACCGACACACTCGACCTGGATCGCGCCGTCGCCGCACTGACGCAGGGCGGCGTGATCGCCTACCCGA
CCGAAGCGGTGTGGGGACTG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 830; Mature: 829

Protein sequence:

>830_residues
MPKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPDNGFAMRYDLIEKNEKHVEAIARAAKGADDIFLA
TDPDREGEAISWHIAEILKERGLLKDKPMQRVVFTEITPRAIKEAMAKPRMIAGDLVDAQQARRALDYLVGFNLSPVLWR
KVQRGLSAGRVQSPALRMIVEREEEIEAFIPREYWSIDAHCRHPSQAFNARLIKLDGQKFEQFTVTDGDTAEAARLRIQQ
AAQGVLHVTDVASKERKRRPAPPFTTSTLQQEASRKLGFTTRKTMQVAQKLYEGVALGDEGSVGLISYMRTDSVNLSQDA
LAEIRDVIARDFGTASLPDQPNAYTTKSKNAQEAHEAVRPTSALRTPAQVARFLSEDERRLYELIWRRAVACQMIPATLN
TVSVDLSAGSEHVFRASGTTVVVPGFLAVYEEGKDTKSSEDEDEGRKLPLMKAGDNVPLDRIVTDQHFTQPPPRFTEAAL
VKALEEYGIGRPSTYASIIQTLQFRKYVEMEGRSFRPTDVGRAVSKFLSGHFTRYVDYDFTANLEDDLDAVSRGEAEWIP
LMEKFWGPFKELVEDKKDSLDKTDAGSVRVLGTDPVSGKEVSARIGRFGPMVQIGTVEDEDKPTFASLRPGQSIYSISLE
DALELFKMPRALGQDKDQDVSVGIGRFGPFARRGSTYASLKKEDDPYTIDLARAIFLIEEKEEIARNRVIKEFDGSDIQV
LNGRFGPYISDGKLNGKIPKDREPASLTFEEVQQLLADTGKPVRKGFGAKKATLKKNTVKDSAPKKPAVKKTATKTAASK
TAVKKAPAKKTATKKAAKRVVKKTVSKAAG

Sequences:

>Translated_830_residues
MPKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPDNGFAMRYDLIEKNEKHVEAIARAAKGADDIFLA
TDPDREGEAISWHIAEILKERGLLKDKPMQRVVFTEITPRAIKEAMAKPRMIAGDLVDAQQARRALDYLVGFNLSPVLWR
KVQRGLSAGRVQSPALRMIVEREEEIEAFIPREYWSIDAHCRHPSQAFNARLIKLDGQKFEQFTVTDGDTAEAARLRIQQ
AAQGVLHVTDVASKERKRRPAPPFTTSTLQQEASRKLGFTTRKTMQVAQKLYEGVALGDEGSVGLISYMRTDSVNLSQDA
LAEIRDVIARDFGTASLPDQPNAYTTKSKNAQEAHEAVRPTSALRTPAQVARFLSEDERRLYELIWRRAVACQMIPATLN
TVSVDLSAGSEHVFRASGTTVVVPGFLAVYEEGKDTKSSEDEDEGRKLPLMKAGDNVPLDRIVTDQHFTQPPPRFTEAAL
VKALEEYGIGRPSTYASIIQTLQFRKYVEMEGRSFRPTDVGRAVSKFLSGHFTRYVDYDFTANLEDDLDAVSRGEAEWIP
LMEKFWGPFKELVEDKKDSLDKTDAGSVRVLGTDPVSGKEVSARIGRFGPMVQIGTVEDEDKPTFASLRPGQSIYSISLE
DALELFKMPRALGQDKDQDVSVGIGRFGPFARRGSTYASLKKEDDPYTIDLARAIFLIEEKEEIARNRVIKEFDGSDIQV
LNGRFGPYISDGKLNGKIPKDREPASLTFEEVQQLLADTGKPVRKGFGAKKATLKKNTVKDSAPKKPAVKKTATKTAASK
TAVKKAPAKKTATKKAAKRVVKKTVSKAAG
>Mature_829_residues
PKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPDNGFAMRYDLIEKNEKHVEAIARAAKGADDIFLAT
DPDREGEAISWHIAEILKERGLLKDKPMQRVVFTEITPRAIKEAMAKPRMIAGDLVDAQQARRALDYLVGFNLSPVLWRK
VQRGLSAGRVQSPALRMIVEREEEIEAFIPREYWSIDAHCRHPSQAFNARLIKLDGQKFEQFTVTDGDTAEAARLRIQQA
AQGVLHVTDVASKERKRRPAPPFTTSTLQQEASRKLGFTTRKTMQVAQKLYEGVALGDEGSVGLISYMRTDSVNLSQDAL
AEIRDVIARDFGTASLPDQPNAYTTKSKNAQEAHEAVRPTSALRTPAQVARFLSEDERRLYELIWRRAVACQMIPATLNT
VSVDLSAGSEHVFRASGTTVVVPGFLAVYEEGKDTKSSEDEDEGRKLPLMKAGDNVPLDRIVTDQHFTQPPPRFTEAALV
KALEEYGIGRPSTYASIIQTLQFRKYVEMEGRSFRPTDVGRAVSKFLSGHFTRYVDYDFTANLEDDLDAVSRGEAEWIPL
MEKFWGPFKELVEDKKDSLDKTDAGSVRVLGTDPVSGKEVSARIGRFGPMVQIGTVEDEDKPTFASLRPGQSIYSISLED
ALELFKMPRALGQDKDQDVSVGIGRFGPFARRGSTYASLKKEDDPYTIDLARAIFLIEEKEEIARNRVIKEFDGSDIQVL
NGRFGPYISDGKLNGKIPKDREPASLTFEEVQQLLADTGKPVRKGFGAKKATLKKNTVKDSAPKKPAVKKTATKTAASKT
AVKKAPAKKTATKKAAKRVVKKTVSKAAG

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=510, Percent_Identity=26.078431372549, Blast_Score=127, Evalue=6e-29,
Organism=Homo sapiens, GI4507635, Length=689, Percent_Identity=24.3831640058055, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1787529, Length=610, Percent_Identity=42.6229508196721, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1788061, Length=562, Percent_Identity=27.5800711743772, Blast_Score=147, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32563869, Length=567, Percent_Identity=28.3950617283951, Blast_Score=145, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17555378, Length=517, Percent_Identity=26.3056092843327, Blast_Score=119, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=519, Percent_Identity=25.626204238921, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24585251, Length=532, Percent_Identity=23.8721804511278, Blast_Score=126, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24640096, Length=520, Percent_Identity=25.7692307692308, Blast_Score=110, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 92017; Mature: 91886

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPDNGFAMRYDLIEKN
CCCCEEEEECCCHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCCCEEEEHHHHCC
EKHVEAIARAAKGADDIFLATDPDREGEAISWHIAEILKERGLLKDKPMQRVVFTEITPR
HHHHHHHHHHHCCCCCEEEECCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHCCHH
AIKEAMAKPRMIAGDLVDAQQARRALDYLVGFNLSPVLWRKVQRGLSAGRVQSPALRMIV
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
EREEEIEAFIPREYWSIDAHCRHPSQAFNARLIKLDGQKFEQFTVTDGDTAEAARLRIQQ
HHHHHHHHHCCHHHCCCCCCCCCCHHHHCCEEEEECCCCCCEEEECCCCHHHHHHHHHHH
AAQGVLHVTDVASKERKRRPAPPFTTSTLQQEASRKLGFTTRKTMQVAQKLYEGVALGDE
HHCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCEECCCC
GSVGLISYMRTDSVNLSQDALAEIRDVIARDFGTASLPDQPNAYTTKSKNAQEAHEAVRP
CCEEEEEEHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
TSALRTPAQVARFLSEDERRLYELIWRRAVACQMIPATLNTVSVDLSAGSEHVFRASGTT
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCE
VVVPGFLAVYEEGKDTKSSEDEDEGRKLPLMKAGDNVPLDRIVTDQHFTQPPPRFTEAAL
EEECCHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHCCCCCCCCCCCCHHHHHH
VKALEEYGIGRPSTYASIIQTLQFRKYVEMEGRSFRPTDVGRAVSKFLSGHFTRYVDYDF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCC
TANLEDDLDAVSRGEAEWIPLMEKFWGPFKELVEDKKDSLDKTDAGSVRVLGTDPVSGKE
CCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHH
VSARIGRFGPMVQIGTVEDEDKPTFASLRPGQSIYSISLEDALELFKMPRALGQDKDQDV
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCC
SVGIGRFGPFARRGSTYASLKKEDDPYTIDLARAIFLIEEKEEIARNRVIKEFDGSDIQV
EEECCCCCCHHHCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LNGRFGPYISDGKLNGKIPKDREPASLTFEEVQQLLADTGKPVRKGFGAKKATLKKNTVK
ECCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHCCCC
DSAPKKPAVKKTATKTAASKTAVKKAPAKKTATKKAAKRVVKKTVSKAAG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPDNGFAMRYDLIEKN
CCCEEEEECCCHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCCCEEEEHHHHCC
EKHVEAIARAAKGADDIFLATDPDREGEAISWHIAEILKERGLLKDKPMQRVVFTEITPR
HHHHHHHHHHHCCCCCEEEECCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHCCHH
AIKEAMAKPRMIAGDLVDAQQARRALDYLVGFNLSPVLWRKVQRGLSAGRVQSPALRMIV
HHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
EREEEIEAFIPREYWSIDAHCRHPSQAFNARLIKLDGQKFEQFTVTDGDTAEAARLRIQQ
HHHHHHHHHCCHHHCCCCCCCCCCHHHHCCEEEEECCCCCCEEEECCCCHHHHHHHHHHH
AAQGVLHVTDVASKERKRRPAPPFTTSTLQQEASRKLGFTTRKTMQVAQKLYEGVALGDE
HHCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCEECCCC
GSVGLISYMRTDSVNLSQDALAEIRDVIARDFGTASLPDQPNAYTTKSKNAQEAHEAVRP
CCEEEEEEHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
TSALRTPAQVARFLSEDERRLYELIWRRAVACQMIPATLNTVSVDLSAGSEHVFRASGTT
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCE
VVVPGFLAVYEEGKDTKSSEDEDEGRKLPLMKAGDNVPLDRIVTDQHFTQPPPRFTEAAL
EEECCHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHCCCCCCCCCCCCHHHHHH
VKALEEYGIGRPSTYASIIQTLQFRKYVEMEGRSFRPTDVGRAVSKFLSGHFTRYVDYDF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCC
TANLEDDLDAVSRGEAEWIPLMEKFWGPFKELVEDKKDSLDKTDAGSVRVLGTDPVSGKE
CCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHH
VSARIGRFGPMVQIGTVEDEDKPTFASLRPGQSIYSISLEDALELFKMPRALGQDKDQDV
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCC
SVGIGRFGPFARRGSTYASLKKEDDPYTIDLARAIFLIEEKEEIARNRVIKEFDGSDIQV
EEECCCCCCHHHCCCCHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LNGRFGPYISDGKLNGKIPKDREPASLTFEEVQQLLADTGKPVRKGFGAKKATLKKNTVK
ECCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCHHHHHHCCCC
DSAPKKPAVKKTATKTAASKTAVKKAPAKKTATKKAAKRVVKKTVSKAAG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]