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The map label for this gene is pilD [H]

Identifier: 21243969

GI number: 21243969

Start: 3821670

End: 3822533

Strand: Direct

Name: pilD [H]

Synonym: XAC3243

Alternate gene names: 21243969

Gene position: 3821670-3822533 (Clockwise)

Preceding gene: 77748708

Following gene: 21243970

Centisome position: 73.84

GC content: 64.24

Gene sequence:

>864_bases
ATGGCATTTCTCGACCAGCATCCCGGTCTCGGCTTTCCTGCCGCGGCCGGACTGGGACTGCTGATCGGCAGCTTCCTGAA
CGTGGTGATCCTGCGCTTGCCCAAGCGTATGGAGTGGCAATGGCGGCGGGATGCGCGCGAGATTTTGGAACTGCCCGATA
TCTACGAGCCGCCGCCGCCCGGGATCGTGGTGGAGCCGTCGCACGACCCGGTGACAGGCGACAAGCTCAAGTGGTGGGAA
AACATTCCGGTCCTGAGCTGGGCGATGCTGCGCGGCAAGTCACGCTACAGCGGCAAGCCGATCTCCATCCAGTATCCACT
GGTCGAGTTGCTCACCTCCATCCTGTGCGTCGCCAGCGTGTGGCGCTTCGGCTTTGGCTGGCAGGGCTTCGGCGCGATCG
TGCTGAGCTGCTTTCTTGTGGCGATGTCGGGCATCGACCTGCGCCACAAGCTGCTGCCCGACCAGTTGACCCTGCCGTTG
ATGTGGTTGGGGCTCGTCGGATCGATGGACAACCTCTACATGCCGGCCAAGCCTGCTCTGCTGGGCGCGGCGGTCGGCTA
TGTGTCGCTATGGACGGTGTGGTGGTTGTTCAAGCAGCTCACCGGCAAGGAAGGCATGGGCCACGGCGACTTCAAGTTGC
TGGCCGCGCTTGGCGCGTGGTGCGGGTTGAAAGGCATCCTGCCGATCATCCTGATCTCCTCGCTCGTCGGCGCCATCCTC
GGCTCGATCTGGCTGGTGGCCAAGGGCCGCGACCGCGCCACCCCCATCCCGTTCGGCCCCTACCTGGCCATTGCTGGCTG
GGTGGTGTTCTTTTGGGGCAACGACCTGGTGGACGGCTACCTGCACTTCGCAGGCCTGCGTTGA

Upstream 100 bases:

>100_bases
ACCCCTGATCATGGTGTTCATTGGTACCATCGTCGGCGGCATGGTCATCGGCATGTACCTGCCTATCTTCAAGCTCGCCT
CGGTGGTTGGATAAGACGTA

Downstream 100 bases:

>100_bases
CCCTGGCACGACGATGAGCGACTTCATCGTCGGCCTTACGGGCGGCATCGCCTCCGGCAAGAGCGCGCTGGCCGCCGAGT
TCGAGAAGCTGGGTGTACCG

Product: type IV pre-pilin leader peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAFLDQHPGLGFPAAAGLGLLIGSFLNVVILRLPKRMEWQWRRDAREILELPDIYEPPPPGIVVEPSHDPVTGDKLKWWE
NIPVLSWAMLRGKSRYSGKPISIQYPLVELLTSILCVASVWRFGFGWQGFGAIVLSCFLVAMSGIDLRHKLLPDQLTLPL
MWLGLVGSMDNLYMPAKPALLGAAVGYVSLWTVWWLFKQLTGKEGMGHGDFKLLAALGAWCGLKGILPIILISSLVGAIL
GSIWLVAKGRDRATPIPFGPYLAIAGWVVFFWGNDLVDGYLHFAGLR

Sequences:

>Translated_287_residues
MAFLDQHPGLGFPAAAGLGLLIGSFLNVVILRLPKRMEWQWRRDAREILELPDIYEPPPPGIVVEPSHDPVTGDKLKWWE
NIPVLSWAMLRGKSRYSGKPISIQYPLVELLTSILCVASVWRFGFGWQGFGAIVLSCFLVAMSGIDLRHKLLPDQLTLPL
MWLGLVGSMDNLYMPAKPALLGAAVGYVSLWTVWWLFKQLTGKEGMGHGDFKLLAALGAWCGLKGILPIILISSLVGAIL
GSIWLVAKGRDRATPIPFGPYLAIAGWVVFFWGNDLVDGYLHFAGLR
>Mature_286_residues
AFLDQHPGLGFPAAAGLGLLIGSFLNVVILRLPKRMEWQWRRDAREILELPDIYEPPPPGIVVEPSHDPVTGDKLKWWEN
IPVLSWAMLRGKSRYSGKPISIQYPLVELLTSILCVASVWRFGFGWQGFGAIVLSCFLVAMSGIDLRHKLLPDQLTLPLM
WLGLVGSMDNLYMPAKPALLGAAVGYVSLWTVWWLFKQLTGKEGMGHGDFKLLAALGAWCGLKGILPIILISSLVGAILG
SIWLVAKGRDRATPIPFGPYLAIAGWVVFFWGNDLVDGYLHFAGLR

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=256, Percent_Identity=34.765625, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789732, Length=128, Percent_Identity=47.65625, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31700; Mature: 31569

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLDQHPGLGFPAAAGLGLLIGSFLNVVILRLPKRMEWQWRRDAREILELPDIYEPPPP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GIVVEPSHDPVTGDKLKWWENIPVLSWAMLRGKSRYSGKPISIQYPLVELLTSILCVASV
CEEECCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
WRFGFGWQGFGAIVLSCFLVAMSGIDLRHKLLPDQLTLPLMWLGLVGSMDNLYMPAKPAL
HHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
LGAAVGYVSLWTVWWLFKQLTGKEGMGHGDFKLLAALGAWCGLKGILPIILISSLVGAIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSIWLVAKGRDRATPIPFGPYLAIAGWVVFFWGNDLVDGYLHFAGLR
HHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AFLDQHPGLGFPAAAGLGLLIGSFLNVVILRLPKRMEWQWRRDAREILELPDIYEPPPP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GIVVEPSHDPVTGDKLKWWENIPVLSWAMLRGKSRYSGKPISIQYPLVELLTSILCVASV
CEEECCCCCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHH
WRFGFGWQGFGAIVLSCFLVAMSGIDLRHKLLPDQLTLPLMWLGLVGSMDNLYMPAKPAL
HHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHH
LGAAVGYVSLWTVWWLFKQLTGKEGMGHGDFKLLAALGAWCGLKGILPIILISSLVGAIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSIWLVAKGRDRATPIPFGPYLAIAGWVVFFWGNDLVDGYLHFAGLR
HHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8817497; 12024217 [H]