Click here to switch to the map view.

The map label for this gene is pilR [H]

Identifier: 21243964

GI number: 21243964

Start: 3814656

End: 3816110

Strand: Direct

Name: pilR [H]

Synonym: XAC3238

Alternate gene names: 21243964

Gene position: 3814656-3816110 (Clockwise)

Preceding gene: 21243963

Following gene: 77748708

Centisome position: 73.71

GC content: 65.43

Gene sequence:

>1455_bases
TTGGCCCGCCACCTGCTCGACAATTGTCGACCATCATCACGCTGGGCTATCGTGCGTCCCATGAACGAACCCAAAAGCGC
CCTGGTTGTCGATGACGAGCGTGATATCCGCGAGTTGCTTGTTCTCACCCTGGGCCGCATGGGCCTGCGCATCAGCACTG
CTGCCAACCTGGCCGAAGCGCGCGAGTTGCTGGCCAACAATCCCTACGATCTGTGCCTGACCGACATGCGTTTGCCGGAC
GGCAACGGCATCGAACTGGTCACCGAAATCGCAAAACACTATCCGCAGACGCCGGTGGCGATGATCACCGCGTTCGGCAG
CATGGACCTGGCCGTGGAAGCACTGAAAGCCGGCGCCTTCGATTTCGTCAGCAAGCCGGTCGACATCGGCGTACTGCGCG
GCCTGGTCAAGCACGCGCTGGAATTGAACAACCGCGACCGCCCTGCTCCGCCACCGCCGCCACCGGAACAGGCCAGCCGC
CTGCTCGGCGACTCCAGTGCCATGGAAATCCTGCGCGCCACCATCAGCAAGGTCGCGCGCAGCCAGGCGCCGGTCTACAT
CGTCGGCGAATCGGGCGTGGGCAAGGAGCTGGTCGCACGCACCATCCACGAACAAGGCGCACGTGCCGCCGGCCCGTTCG
TGCCGGTCAACTGCGGCGCCATTCCCGCCGAGCTGATGGAAAGCGAATTCTTCGGCCACAAGAAAGGCAGCTTCACCGGC
GCGCATGCCGACAAGCCCGGGCTGTTCCAGGCCGCGCATGGAGGCACGCTGTTTCTGGACGAAGTGGCCGAATTGCCGTT
GCAGATGCAGGTCAAGCTGCTGCGCGCGATCCAGGAAAAGTCGGTGCGACCGGTCGGCGCATCCAGCGAATCGCTGGTGG
ATGTGCGCATCCTCTCGGCCACCCACAAGGACCTGGGCGACCTGGTCTCCGACGGCCGCTTTCGGCATGACCTGTACTAC
CGCATCAACGTGATCGAGTTGCGTGTGCCGCCGCTGCGCGAACGCGGTGGCGACCTGCCGCAGCTGGCCGCTGCCATCAT
CGCGCGCCTGGCGCACAGCCACGGCCGCCCCATTCCGCTGCTGACCCAATCCGCACTCGACGCCTTGAATCACTACGGCT
TCCCCGGCAACGTGCGCGAACTGGGAAACATCCTCGAACGCGCCCTGGCCCTGGCCGAAGACGACCAGATCAGCGCTACC
GACCTGCGCCTGCCCGCGCACGGGGGCCACCGGTTGGCCGCCCCCCCAGGAGGCGCCGCGGCCGAACCGCGCGAAGCCGT
CGTCGACATCGACCCCGCCTCGGCCGCGCTGCCTTCCTACATCGAGCAACTGGAACGCGCCGCGATCCAGAAGGCGCTGG
AAGAAAACCGCTGGAACAAGACCAAGACTGCTGCGCAGTTGGGCATCACGTTTCGGGCGCTGCGTTACAAGCTGAAGAAG
CTGGGGATGGAGTAG

Upstream 100 bases:

>100_bases
GTTTGGCAACGCAGCGCATCGACAGCCATCGCCGACTGGCATCGGCAGCTTGCAAACGCGCTGAAAGCCGCAGTGACGCG
CCCGCGCAGCAGGTGAATCG

Downstream 100 bases:

>100_bases
AAGAGCAGGGAGTCGGGAGTGGGGAATCGGGAATCGTAAAAGCGCAGTTGCCGGCCTCGATGGCCTGTGTGGGAGACACT
GCGATGGACTGGCTCATGCG

Product: two-component system regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MARHLLDNCRPSSRWAIVRPMNEPKSALVVDDERDIRELLVLTLGRMGLRISTAANLAEARELLANNPYDLCLTDMRLPD
GNGIELVTEIAKHYPQTPVAMITAFGSMDLAVEALKAGAFDFVSKPVDIGVLRGLVKHALELNNRDRPAPPPPPPEQASR
LLGDSSAMEILRATISKVARSQAPVYIVGESGVGKELVARTIHEQGARAAGPFVPVNCGAIPAELMESEFFGHKKGSFTG
AHADKPGLFQAAHGGTLFLDEVAELPLQMQVKLLRAIQEKSVRPVGASSESLVDVRILSATHKDLGDLVSDGRFRHDLYY
RINVIELRVPPLRERGGDLPQLAAAIIARLAHSHGRPIPLLTQSALDALNHYGFPGNVRELGNILERALALAEDDQISAT
DLRLPAHGGHRLAAPPGGAAAEPREAVVDIDPASAALPSYIEQLERAAIQKALEENRWNKTKTAAQLGITFRALRYKLKK
LGME

Sequences:

>Translated_484_residues
MARHLLDNCRPSSRWAIVRPMNEPKSALVVDDERDIRELLVLTLGRMGLRISTAANLAEARELLANNPYDLCLTDMRLPD
GNGIELVTEIAKHYPQTPVAMITAFGSMDLAVEALKAGAFDFVSKPVDIGVLRGLVKHALELNNRDRPAPPPPPPEQASR
LLGDSSAMEILRATISKVARSQAPVYIVGESGVGKELVARTIHEQGARAAGPFVPVNCGAIPAELMESEFFGHKKGSFTG
AHADKPGLFQAAHGGTLFLDEVAELPLQMQVKLLRAIQEKSVRPVGASSESLVDVRILSATHKDLGDLVSDGRFRHDLYY
RINVIELRVPPLRERGGDLPQLAAAIIARLAHSHGRPIPLLTQSALDALNHYGFPGNVRELGNILERALALAEDDQISAT
DLRLPAHGGHRLAAPPGGAAAEPREAVVDIDPASAALPSYIEQLERAAIQKALEENRWNKTKTAAQLGITFRALRYKLKK
LGME
>Mature_483_residues
ARHLLDNCRPSSRWAIVRPMNEPKSALVVDDERDIRELLVLTLGRMGLRISTAANLAEARELLANNPYDLCLTDMRLPDG
NGIELVTEIAKHYPQTPVAMITAFGSMDLAVEALKAGAFDFVSKPVDIGVLRGLVKHALELNNRDRPAPPPPPPEQASRL
LGDSSAMEILRATISKVARSQAPVYIVGESGVGKELVARTIHEQGARAAGPFVPVNCGAIPAELMESEFFGHKKGSFTGA
HADKPGLFQAAHGGTLFLDEVAELPLQMQVKLLRAIQEKSVRPVGASSESLVDVRILSATHKDLGDLVSDGRFRHDLYYR
INVIELRVPPLRERGGDLPQLAAAIIARLAHSHGRPIPLLTQSALDALNHYGFPGNVRELGNILERALALAEDDQISATD
LRLPAHGGHRLAAPPGGAAAEPREAVVDIDPASAALPSYIEQLERAAIQKALEENRWNKTKTAAQLGITFRALRYKLKKL
GME

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=465, Percent_Identity=39.1397849462366, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI1790437, Length=453, Percent_Identity=41.280353200883, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=37.2844827586207, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1788905, Length=462, Percent_Identity=37.2294372294372, Blast_Score=278, Evalue=5e-76,
Organism=Escherichia coli, GI1789233, Length=333, Percent_Identity=39.9399399399399, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI87082117, Length=238, Percent_Identity=46.218487394958, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1789087, Length=247, Percent_Identity=44.5344129554656, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87082152, Length=328, Percent_Identity=40.2439024390244, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786524, Length=294, Percent_Identity=41.156462585034, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI87081872, Length=326, Percent_Identity=35.5828220858896, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=35.0769230769231, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789828, Length=233, Percent_Identity=37.7682403433476, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87081858, Length=309, Percent_Identity=30.7443365695793, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1786911, Length=147, Percent_Identity=32.6530612244898, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1787487, Length=152, Percent_Identity=33.5526315789474, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52467; Mature: 52336

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARHLLDNCRPSSRWAIVRPMNEPKSALVVDDERDIRELLVLTLGRMGLRISTAANLAEA
CCCHHHHHCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHCCEEEHHHHHHHHH
RELLANNPYDLCLTDMRLPDGNGIELVTEIAKHYPQTPVAMITAFGSMDLAVEALKAGAF
HHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCCH
DFVSKPVDIGVLRGLVKHALELNNRDRPAPPPPPPEQASRLLGDSSAMEILRATISKVAR
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
SQAPVYIVGESGVGKELVARTIHEQGARAAGPFVPVNCGAIPAELMESEFFGHKKGSFTG
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCC
AHADKPGLFQAAHGGTLFLDEVAELPLQMQVKLLRAIQEKSVRPVGASSESLVDVRILSA
CCCCCCCCEEECCCCEEEHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHH
THKDLGDLVSDGRFRHDLYYRINVIELRVPPLRERGGDLPQLAAAIIARLAHSHGRPIPL
HHHHHHHHHHCCCEEEEEEEEEEEEEEECCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCH
LTQSALDALNHYGFPGNVRELGNILERALALAEDDQISATDLRLPAHGGHRLAAPPGGAA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEECCCCCCCC
AEPREAVVDIDPASAALPSYIEQLERAAIQKALEENRWNKTKTAAQLGITFRALRYKLKK
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGME
HCCC
>Mature Secondary Structure 
ARHLLDNCRPSSRWAIVRPMNEPKSALVVDDERDIRELLVLTLGRMGLRISTAANLAEA
CCHHHHHCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHCCEEEHHHHHHHHH
RELLANNPYDLCLTDMRLPDGNGIELVTEIAKHYPQTPVAMITAFGSMDLAVEALKAGAF
HHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHCCCH
DFVSKPVDIGVLRGLVKHALELNNRDRPAPPPPPPEQASRLLGDSSAMEILRATISKVAR
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
SQAPVYIVGESGVGKELVARTIHEQGARAAGPFVPVNCGAIPAELMESEFFGHKKGSFTG
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCC
AHADKPGLFQAAHGGTLFLDEVAELPLQMQVKLLRAIQEKSVRPVGASSESLVDVRILSA
CCCCCCCCEEECCCCEEEHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHH
THKDLGDLVSDGRFRHDLYYRINVIELRVPPLRERGGDLPQLAAAIIARLAHSHGRPIPL
HHHHHHHHHHCCCEEEEEEEEEEEEEEECCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCH
LTQSALDALNHYGFPGNVRELGNILERALALAEDDQISATDLRLPAHGGHRLAAPPGGAA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCEECCCCCCCC
AEPREAVVDIDPASAALPSYIEQLERAAIQKALEENRWNKTKTAAQLGITFRALRYKLKK
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LGME
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]