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The map label for this gene is pilS [H]

Identifier: 21243963

GI number: 21243963

Start: 3812778

End: 3814391

Strand: Direct

Name: pilS [H]

Synonym: XAC3237

Alternate gene names: 21243963

Gene position: 3812778-3814391 (Clockwise)

Preceding gene: 21243959

Following gene: 21243964

Centisome position: 73.67

GC content: 67.41

Gene sequence:

>1614_bases
TTGGCATCCATCGCATCGCTCATCGCCCGGATCCAGTCCGCGCCGCAGCGCGAGCTGTACTTTTTCTCGCTGTACCGGGT
ACTGGTGGCCGGCCTGATCGCCGCGCTGGTGTTCAGCCCCTTGTCCGACGCCATCCCCGAGCCGCGCTACCCGCGCCTGG
CCGCCGGCGTGGCGATCGGCTACCTGTGCATGGCGATCCCGCTGCTGTTCTGGGGCCGCGGCGAACGTCGCCTTACCCCC
ACCGTGCTGTGCGCGGTAGTGGCCGACATCCTTGCCGCCACCCTGGCCACGCACGCCCTGCCCGGCGCCAGCGCCGGCAT
TGCGATGATGCTGCTGTTCAACGTGGCGGCCGCCTCCATCCTGCTGCGACTGCGCTACGGCATGAGCATCGCGGTACTCG
CCAGCGTCGCCATCCTGCTGGAATACCTGTGGACCCTGCTCGAAGGCGGCGGCGCCACCCGGCCGGTGGCGGAGCTTGCG
ATGTTTGCCACCAGCTACGTCGCGGTGGCCTTCATCTGCGAACAAATCGCCGCGCGCGCCCGTCGCAACCAGATGCTGGC
CGAAGAACGCGGCGCCCAGGTCGCCAATCTGTACGAGATCAACGAGCTGATCATCCGGCGCATGCGCACCGGCGTGCTGG
TGGTGGATACCCACAACCACATCTCGTTGGCCAACGAAGCGGCGCTGAGCTTGCTGGGCGATGGCGACCAACGCACCCCG
TCCACCGATCTGTCGCTGGTGGCGCTCACCCCCGAACTGGCGCGCCGGCTGCAGCGCTGGCGTAGCGGCTGGCGCGAGGA
AGAGGCGCCGCTGCAACTCGGCGCCGAACGCCCGGAAGTGCAACCGCGCTTCGTGCGCCTGCTTGCCGACAGCGACCTGG
CACTGGTGTTCCTGGACGACGCCACGGTGGTGTCGCGACGTGCCGAATCGCTGACCCTGTCGGCCATGGGGCGCTTTTCG
GCCAGCTTGGCGCACGAGATCCGCAACCCGCTGGCCGCAATCAGCTACGCCTCCCAATTGCTGGAAGAATCGCCGGACAT
CGGCGATGCCGACCGCCGCCTGCTGCAGATCATCCACCAGCAATGCCAGCGCACCAACGGCATCGTCGAGAGCGTCCTCG
CGCTGGCGCGCCGGGAACGCGCCACCCCGGACAATCTGGACCTGGCCGCATTCGTGCGCCGCTTCGTGGTCGAATATCGC
CAGACCTTGTCGATGGAAACCGACATCCTGGAGGCCAGCATCCAGGGGAGCTCCGTGCATGCGTTGGTCGACCCCAAGCA
CCTGCATCAGATCCTCACCGCGCTGGTGCACAACGCACTCAAGTACGGCCGGGTGATGGACGAACCGGCACGCGTCAAGC
TGCACGTCGAACGCCTCGAGCGCAAGGCAGTGATCGATGTGATCGACCGCGGCCCAGGCATCCCCGACGCCGTGGCGGCA
CAACTGTTCCGCCCGTTCTACACCACCTCCGAGCATGGCACCGGCCTGGGCCTGTACATCGCCCAGGAACTCTGCCGCGC
CAACCAGGCGCAACTGGACTACGTCTCGGTCCCGGGCGGCGGCGCGTGCTTCCGGATTTCGTTGCAAGGCCCCAACGGAT
TGCTCGGCAAGTGA

Upstream 100 bases:

>100_bases
GCCCGATCGCAGCGCTTGCGCGCAGGCCGACTCTGTGCGCAGGCGCTGCGCAGACCGCGCACCTGCCTATACTCGCGGCT
CGCTCCAGCGGGGAATCGGC

Downstream 100 bases:

>100_bases
GTGCTCGGCTGTCGGCTGAGCGGGATGCCTGATGTGGCGATACCCAGCAGAGCAGCGACTTGTTCGATCGCAGAACATCG
CATTGGCGAAGACGCAAACG

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MASIASLIARIQSAPQRELYFFSLYRVLVAGLIAALVFSPLSDAIPEPRYPRLAAGVAIGYLCMAIPLLFWGRGERRLTP
TVLCAVVADILAATLATHALPGASAGIAMMLLFNVAAASILLRLRYGMSIAVLASVAILLEYLWTLLEGGGATRPVAELA
MFATSYVAVAFICEQIAARARRNQMLAEERGAQVANLYEINELIIRRMRTGVLVVDTHNHISLANEAALSLLGDGDQRTP
STDLSLVALTPELARRLQRWRSGWREEEAPLQLGAERPEVQPRFVRLLADSDLALVFLDDATVVSRRAESLTLSAMGRFS
ASLAHEIRNPLAAISYASQLLEESPDIGDADRRLLQIIHQQCQRTNGIVESVLALARRERATPDNLDLAAFVRRFVVEYR
QTLSMETDILEASIQGSSVHALVDPKHLHQILTALVHNALKYGRVMDEPARVKLHVERLERKAVIDVIDRGPGIPDAVAA
QLFRPFYTTSEHGTGLGLYIAQELCRANQAQLDYVSVPGGGACFRISLQGPNGLLGK

Sequences:

>Translated_537_residues
MASIASLIARIQSAPQRELYFFSLYRVLVAGLIAALVFSPLSDAIPEPRYPRLAAGVAIGYLCMAIPLLFWGRGERRLTP
TVLCAVVADILAATLATHALPGASAGIAMMLLFNVAAASILLRLRYGMSIAVLASVAILLEYLWTLLEGGGATRPVAELA
MFATSYVAVAFICEQIAARARRNQMLAEERGAQVANLYEINELIIRRMRTGVLVVDTHNHISLANEAALSLLGDGDQRTP
STDLSLVALTPELARRLQRWRSGWREEEAPLQLGAERPEVQPRFVRLLADSDLALVFLDDATVVSRRAESLTLSAMGRFS
ASLAHEIRNPLAAISYASQLLEESPDIGDADRRLLQIIHQQCQRTNGIVESVLALARRERATPDNLDLAAFVRRFVVEYR
QTLSMETDILEASIQGSSVHALVDPKHLHQILTALVHNALKYGRVMDEPARVKLHVERLERKAVIDVIDRGPGIPDAVAA
QLFRPFYTTSEHGTGLGLYIAQELCRANQAQLDYVSVPGGGACFRISLQGPNGLLGK
>Mature_536_residues
ASIASLIARIQSAPQRELYFFSLYRVLVAGLIAALVFSPLSDAIPEPRYPRLAAGVAIGYLCMAIPLLFWGRGERRLTPT
VLCAVVADILAATLATHALPGASAGIAMMLLFNVAAASILLRLRYGMSIAVLASVAILLEYLWTLLEGGGATRPVAELAM
FATSYVAVAFICEQIAARARRNQMLAEERGAQVANLYEINELIIRRMRTGVLVVDTHNHISLANEAALSLLGDGDQRTPS
TDLSLVALTPELARRLQRWRSGWREEEAPLQLGAERPEVQPRFVRLLADSDLALVFLDDATVVSRRAESLTLSAMGRFSA
SLAHEIRNPLAAISYASQLLEESPDIGDADRRLLQIIHQQCQRTNGIVESVLALARRERATPDNLDLAAFVRRFVVEYRQ
TLSMETDILEASIQGSSVHALVDPKHLHQILTALVHNALKYGRVMDEPARVKLHVERLERKAVIDVIDRGPGIPDAVAAQ
LFRPFYTTSEHGTGLGLYIAQELCRANQAQLDYVSVPGGGACFRISLQGPNGLLGK

Specific function: Member of the two-component regulatory system pilS/pilR involved in the regulation of the expression of type 4 fimbriae. PilS may function as a membrane-associated protein kinase that phosphorylates pilR in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=246, Percent_Identity=29.2682926829268, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1790436, Length=228, Percent_Identity=26.7543859649123, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI87082128, Length=222, Percent_Identity=26.5765765765766, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790300, Length=361, Percent_Identity=27.4238227146814, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790861, Length=219, Percent_Identity=27.3972602739726, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1786600, Length=219, Percent_Identity=28.7671232876712, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI87081816, Length=237, Percent_Identity=29.535864978903, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788713, Length=236, Percent_Identity=25.8474576271186, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58659; Mature: 58528

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASIASLIARIQSAPQRELYFFSLYRVLVAGLIAALVFSPLSDAIPEPRYPRLAAGVAIG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
YLCMAIPLLFWGRGERRLTPTVLCAVVADILAATLATHALPGASAGIAMMLLFNVAAASI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LLRLRYGMSIAVLASVAILLEYLWTLLEGGGATRPVAELAMFATSYVAVAFICEQIAARA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRNQMLAEERGAQVANLYEINELIIRRMRTGVLVVDTHNHISLANEAALSLLGDGDQRTP
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCCC
STDLSLVALTPELARRLQRWRSGWREEEAPLQLGAERPEVQPRFVRLLADSDLALVFLDD
CCCCEEEEECHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHCCCCEEEEEECC
ATVVSRRAESLTLSAMGRFSASLAHEIRNPLAAISYASQLLEESPDIGDADRRLLQIIHQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
QCQRTNGIVESVLALARRERATPDNLDLAAFVRRFVVEYRQTLSMETDILEASIQGSSVH
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ALVDPKHLHQILTALVHNALKYGRVMDEPARVKLHVERLERKAVIDVIDRGPGIPDAVAA
EEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QLFRPFYTTSEHGTGLGLYIAQELCRANQAQLDYVSVPGGGACFRISLQGPNGLLGK
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
ASIASLIARIQSAPQRELYFFSLYRVLVAGLIAALVFSPLSDAIPEPRYPRLAAGVAIG
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
YLCMAIPLLFWGRGERRLTPTVLCAVVADILAATLATHALPGASAGIAMMLLFNVAAASI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LLRLRYGMSIAVLASVAILLEYLWTLLEGGGATRPVAELAMFATSYVAVAFICEQIAARA
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRNQMLAEERGAQVANLYEINELIIRRMRTGVLVVDTHNHISLANEAALSLLGDGDQRTP
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCCC
STDLSLVALTPELARRLQRWRSGWREEEAPLQLGAERPEVQPRFVRLLADSDLALVFLDD
CCCCEEEEECHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHCCCCEEEEEECC
ATVVSRRAESLTLSAMGRFSASLAHEIRNPLAAISYASQLLEESPDIGDADRRLLQIIHQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
QCQRTNGIVESVLALARRERATPDNLDLAAFVRRFVVEYRQTLSMETDILEASIQGSSVH
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
ALVDPKHLHQILTALVHNALKYGRVMDEPARVKLHVERLERKAVIDVIDRGPGIPDAVAA
EEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QLFRPFYTTSEHGTGLGLYIAQELCRANQAQLDYVSVPGGGACFRISLQGPNGLLGK
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8097014; 10984043 [H]